BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0008.p (423 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4R6.1 B3K4R6_9BACI Major facilitator superfamily MFS_1 422 e-116 A7HKV4.1 A7HKV4_FERNB Major facilitator superfamily MFS_1 108 7e-22 B8I3Z9.1 B8I3Z9_CLOCE Major facilitator superfamily MFS_1 86 6e-15 B8GAM4.1 B8GAM4_CHLAD Major facilitator superfamily MFS_1 84 2e-14 C1U1P0.1 C1U1P0_9SPHI Major Facilitator Superfamily transporter 84 2e-14 >B3K4R6.1 B3K4R6_9BACI Major facilitator superfamily MFS_1 Length = 428 Score = 422 bits (1085), Expect = e-116, Method: Compositional matrix adjust. Identities = 205/389 (52%), Positives = 268/389 (68%) Query: 14 KGLRKASVHRKNLQIATFEGIPSTIXXXXXXXXXXXXXXXXXXASSSQIGFVIALTTLVN 73 K ++ S RK+L AT+EG+P+ I A SS++GFV+A+TTL N Sbjct: 18 KPRKQVSHQRKHLMTATWEGVPAIILQTLLGGPFLTGYLLYLGAESSEVGFVLAVTTLFN 77 Query: 74 VAQIGVAFLIQKLPSRKWAMVTFIGLHRVLWSSTGLVPFIFPKEHWVTAFIVLYTIAFIA 133 V QI VA+LIQ+L RK AM+ F +HR+LW +TGL+PFIFP+ WV F++LY AFIA Sbjct: 78 VLQILVAYLIQRLQYRKKAMIVFTFMHRMLWGATGLIPFIFPERWWVPVFMILYMGAFIA 137 Query: 134 NTAGGVLWSSVISDLVPARVRGRYFGIRNTFLNALGSLVMYGGGLVLDRYPGGHGFLILY 193 NT G+LW+S+ISD+VPA+VRGRYFGIRNT LNALG+L ++ GG++LDRYPGG GFLIL+ Sbjct: 138 NTVSGMLWTSLISDMVPAKVRGRYFGIRNTILNALGALTLFVGGIILDRYPGGGGFLILF 197 Query: 194 XXXXXXXXXXXXXXXXYPDVPFEKSEEKDFLPMLKKPLHDTLFMKSTLFLSAWLLLQNLT 253 YPD PFE S E+ F M++KPL DT F+KSTLFL+ WL LQ L Sbjct: 198 MISWIAIAANLSMFFLYPDPPFEHSSERQFRAMIRKPLSDTTFIKSTLFLAGWLFLQTLI 257 Query: 254 VPLYSYVMLQLLHINYETLSLLNVSQTIFMMASFYVWGNLNAKYSNKRLLLWTLPIIAXX 313 VPLYSY ML +LHI+Y +S++ V+QT+ MM FYVWGNLNA++SN+ LL WTLP+IA Sbjct: 258 VPLYSYAMLDVLHISYSIVSIMTVAQTVIMMIGFYVWGNLNARFSNRTLLYWTLPLIAGS 317 Query: 314 XXXXXXXXXXXXXXXXFAAHIVFGVGVGGFNQLAFNFIIGDTPKKERPMYMAMYAALTGL 373 +H++ G GVGGFNQLAFNF+IGDTPK ERPM++A+Y+++TG Sbjct: 318 CLTWGLLSVLPVIPVLLLSHMLLGAGVGGFNQLAFNFMIGDTPKSERPMFIAVYSSITGF 377 Query: 374 AAFFGPLIGGKIYEWIHEWPNWTQVYGMQ 402 A+F GP++GG I++ I P+W YG Q Sbjct: 378 ASFLGPMLGGLIFKGIAHAPDWVSRYGFQ 406 >A7HKV4.1 A7HKV4_FERNB Major facilitator superfamily MFS_1 Length = 454 Score = 108 bits (271), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 89/344 (25%), Positives = 156/344 (45%), Gaps = 10/344 (2%) Query: 62 IGFVIALTTLVNVAQIGVAFLIQKLPSRKWAMVTFIGLHRVLWSSTGLVPFIFPKEHWVT 121 +G V + + QI +I+KLPS+K ++ F R LW L+P +F + Sbjct: 52 LGLVSSFPMAFQIFQIFAPAVIEKLPSKK-KLLAFFNSGRFLW--IILIPLLFSEHRKPE 108 Query: 122 AFIVLYTIAFIANTAGGVLWSSVISDLVPARVRGRYFGIRNTFLNALGSLVMYGGGLVLD 181 FI+++ ++ + G +W S+ISD++P+ RG+Y G+RN F++ LV Y L+ D Sbjct: 109 LFILVFALSQVFAAFAGNVWISIISDVIPSERRGKYLGLRNFFVSLATLLVFYLYSLIAD 168 Query: 182 RYPGGHGFLILYXXXXXXXXXXXXXXXXYPDVPFEKSEEKDFLPMLKKPLHDTLFMKSTL 241 F I+ DV +K+ L L+ L D FMK + Sbjct: 169 NIKKPFNFAIIILITMVTTLFSLVSLMKVRDVSSKKTGS---LNDLRIVLKDKNFMKLSK 225 Query: 242 FLSAWLLLQNLTVPLYSYVMLQLLHINYETLSLLNVSQTIFMMASFYVWGNLNAKYSNKR 301 W + L+ P + Y + L + +S +++ ++ M + +WG L+ ++ +K Sbjct: 226 AYFMWNFVILLSAPFFPYHQIHNLKLPMTYISYASITASLLSMLFYTLWGRLSDEFGHKS 285 Query: 302 LLLWTLPIIAXXXXXXXXXXXXXXXXXXFAAHIVFGVGVGGFNQLAFNFIIGDTPKKERP 361 +L+ L I++ I+ GVG N LAF + +T P Sbjct: 286 VLITGLSIVSITPAIWILMNEKHWVLALTLDAILSGVGWAAVN-LAFITLPMETASSSSP 344 Query: 362 MYMAMYAALTGLAAFFGPLIGGKIYEWIHEWPNWTQ---VYGMQ 402 MY A+++AL GL G LIGG I ++ + + + ++G+Q Sbjct: 345 MYFAVFSALGGLGGMIGSLIGGPIAKFFNSLDFYVKDYHIFGLQ 388 >B8I3Z9.1 B8I3Z9_CLOCE Major facilitator superfamily MFS_1 Length = 446 Score = 86.3 bits (212), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 82/342 (23%), Positives = 144/342 (42%), Gaps = 24/342 (7%) Query: 57 ASSSQIGFVIALTTLVNVAQIGVAFLIQKLPSRKWAMVTFIGLHRVLWSSTGLVPFIFPK 116 AS IG ++A+ L N+ Q+ ++KL SRK +VT +R L ++P + Sbjct: 54 ASDQIIGLIVAMPLLANILQMFSPIFLEKLTSRKRLIVTTSLCYRSLLGLMIVIPLLTQN 113 Query: 117 EH-----WVTAFIVLYTIAFIANTAGGVLWSSVISDLVPARVRGRYFGIRNTFLNALGSL 171 ++ Y I +N AGG S I LVP R RGRYFG+R+TF+ + ++ Sbjct: 114 TSARLLLLAGMYLTAYLIFSFSNPAGG----SWIISLVPERYRGRYFGLRDTFIISSAAV 169 Query: 172 VMYGGGLVLD--RYPGGH--GFLILYXXXXXXXXXXXXXXXXYPD---VPFEKSEEKD-- 222 + G VLD + G GF+I++ + VP +++ Sbjct: 170 LSLSMGRVLDILKVSGKEFLGFIIVFSLVLVLVVLDIYVLNKIREPKIVPIKQNVNVKSL 229 Query: 223 -FLPMLKKPLHDTLFMKSTLFLSAWLLLQNLTVPLYSYVMLQLLHINYETLSLLNVSQTI 281 LP+ K +F+ +T W L +P +S M+ L ++Y + N+ ++ Sbjct: 230 FTLPLKNKQFRPVIFLNAT-----WSFAAQLALPFFSVYMVTGLELSYTFIMAANILMSV 284 Query: 282 FMMASFYVWGNLNAKYSNKRLLLWTLPIIAXXXXXXXXXXXXXXXXXXFAAHIVFGVGVG 341 ++ +WG L K+S + + ++ ++ I+ G G Sbjct: 285 VQASTAKLWGRLADKFSWEVTTIISIGMLGLCHLTWAFVTKEVCYLIIPFIQILAGAGWS 344 Query: 342 GFNQLAFNFIIGDTPKKERPMYMAMYAALTGLAAFFGPLIGG 383 G N FN P++ R +++ AA+ G+ F L+G Sbjct: 345 GVNMSLFNIQFKHAPQEGRTIFVGFNAAIAGVTGFASALLGA 386 >B8GAM4.1 B8GAM4_CHLAD Major facilitator superfamily MFS_1 Length = 445 Score = 84.3 bits (207), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 85/342 (24%), Positives = 145/342 (42%), Gaps = 10/342 (2%) Query: 57 ASSSQIGFVIALTTLVNVAQIGVAFLIQKLPSRKWAMVTFIGLH-RVLWSSTGLVPFI-F 114 A++ QIG + AL + Q A+L + R+ +V + L R++W+ +PF + Sbjct: 71 ANTFQIGLLGALPFIGQFFQFLSAYLETRFAKRR-PIVLYTALTGRLVWAFLLWLPFTGW 129 Query: 115 PKEHWVTAFIVLYTIAFIANTAGGVLWSSVISDLVPARVRGRYFGIRNTFLNALGSLVMY 174 P + F + ++ N G W S +SDLVP+ RG YFG RNT + +Y Sbjct: 130 PAAWQLNMFFIALGCSYALNGMAGNAWMSWMSDLVPSNRRGSYFGWRNTIAGLSSMVSVY 189 Query: 175 GGGLVLDRYPGGH----GFLILYXXXXXXXXXXXXXXXXYPDVPFEKSEEKDFLPMLKKP 230 GL LD + H G+ +++ P+ P + L P Sbjct: 190 LAGLTLDHFRMNHAEAQGYAVIFGVAVVAAFGAALLIARQPEPPLTPRPWEGVGTFLLSP 249 Query: 231 LHDTLFMKSTLFLSAWLLLQNLTVPLYSYVMLQLLHINYETLSLLN-VSQTIFMMASFYV 289 L D +F + T+ +AW L+ + P ++ L L +++ L+L V+ T+ ++ S V Sbjct: 250 LRDDVFRRFTILATAWALVTGIAAPFFNIYGLTTLQLDFSLLALTGIVTNTVALVFSPLV 309 Query: 290 WGNLNAKYSNKRLLLWTLPIIAXXXXXXXXXXXXXXXXXXFAAHIVFGVGVGGFNQLAFN 349 G L +Y RL++ I + I GV G NQ N Sbjct: 310 -GWLMDRY-GYRLVVAVCVIGTIPLPLGWVLSTPHNIVPLWLTSIFSGVFWPGVNQGLGN 367 Query: 350 FIIGDTPKKERPMYMAMYAALTGLAAFFGPLIGGKIYEWIHE 391 ++ P +R MA+++ +TGL L GG + + + + Sbjct: 368 LLMERAPATQRGTAMAIFSLITGLGTLIASLAGGALGQTMSQ 409 >C1U1P0.1 C1U1P0_9SPHI Major Facilitator Superfamily transporter Length = 499 Score = 84.3 bits (207), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 78/343 (22%), Positives = 141/343 (41%), Gaps = 13/343 (3%) Query: 57 ASSSQIGFVIALTTLVNVAQIGVAFLIQKLPSRKWAMVTFIGLHRVLWSSTGLVPFIFPK 116 A++ QIG + +L T N+ Q+ +LI++ +R+ V R+ G +PFIFP Sbjct: 49 ANNFQIGLLASLPTFTNIFQLTSVWLIRRYNNRRAVSVICSVFARIPLILIGTLPFIFPA 108 Query: 117 EHWVTAFIVLYTIAFIANTAGGVLWSSVISDLVPARVRGRYFGIRNTFLNALGSLVMYGG 176 V + ++ + G W++ + DLVP + G YF R + L ++ Sbjct: 109 GAPVNLLLFFLFFFYLFGSIAGPSWNAWMKDLVPGDMLGVYFSKRGRYAQILNVIMSIIL 168 Query: 177 GLVLD----RYPGGH--GFLILYXXXXXXXXXXXXXXXXYPDVPFEKSEEKDFLPMLKKP 230 L +D +P + +++ P+ P ++ + M +KP Sbjct: 169 ALAVDYIRKNFPAFELTTYAVMFMLAGVVGLGGAWLLSTVPE-PTSYLGNENIIRMFQKP 227 Query: 231 LHDTLFMKSTLFLSAWLLLQNLTVPLYSYVMLQLLHINYETLSLLNVSQTIFMMASFYVW 290 L D F + +F SAWL N+ P ++ ML+ L + + L + + + + W Sbjct: 228 LRDLNFRRLLVFNSAWLFAVNIATPFFTVFMLKTLGLTLMYIIPLTILSQLSSIVTIGYW 287 Query: 291 GNLNAKYSNKRLL-----LWTLPIIAXXXXXXXXXXXXXXXXXXFAAHIVFGVGVGGFNQ 345 G +YSNK ++ L+ +IA HI G G N Sbjct: 288 GVFADRYSNKTIISICAPLYLCCVIAWCFVGIYSNLISNLVLLAL-IHIASGASNAGINL 346 Query: 346 LAFNFIIGDTPKKERPMYMAMYAALTGLAAFFGPLIGGKIYEW 388 N + P KE +Y+++ +T + + PLIGG + ++ Sbjct: 347 ALTNIGLKLAPAKEAIIYLSVKNIITSVFSSLAPLIGGYLADY 389 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.142 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,134,530,042 Number of extensions: 79052118 Number of successful extensions: 246493 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 246588 Number of HSP's successfully gapped: 67 Length of query: 423 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 285 Effective length of database: 1,760,596,133 Effective search space: 501769897905 Effective search space used: 501769897905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)