BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0022.p (385 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKR6.1 B3KKR6_9BACI Sporulation protein YunB 307 1e-81 B1DFC9.1 B1DFC9_9BACL Sporulation protein YunB 287 1e-75 C0ZKX3.1 C0ZKX3_BREBN Putative uncharacterized protein yunB 157 2e-36 C0ZJS3.1 C0ZJS3_BREBN Putative uncharacterized protein yunB 135 7e-30 C0GJL1.1 C0GJL1_9FIRM Sporulation protein YunB 121 1e-25 >B3KKR6.1 B3KKR6_9BACI Sporulation protein YunB Length = 320 Score = 307 bits (786), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 138/218 (63%), Positives = 184/218 (84%), Gaps = 2/218 (0%) Query: 104 RSQKPRRRG--RFWLIFSLVLILAVLQSLRYVEKHLKPPILHLAQIRVKQIATESINKAI 161 R Q +RR ++WLI +L+L+ +++ Y+E++L+PPI+HLAQIRVKQIATE+INKAI Sbjct: 41 RPQATKRRSTKKWWLIGALLLVFGIVEFFMYIEQNLRPPIMHLAQIRVKQIATEAINKAI 100 Query: 162 TTQVADTGNAEALIDWKTDKEGKISGFMLNYKEHMRITSQAAEVIQSTLQELHNQTERIP 221 T+QV + + L++WKTD GK++GFMLNY EH++ITS +V+QSTL E+H + E+IP Sbjct: 101 TSQVTQGKDFDNLVNWKTDSSGKVTGFMLNYAEHLKITSHTTQVVQSTLDEIHKRKEQIP 160 Query: 222 LGQALGSSLIASFGPDVPIKIEPQGAVKVELNTRQKDAGINMILVEVYIHIVTEVAVIIP 281 LGQ L S +IASFGP +P+KIEPQGAVKV+L+TRQ+ AGINMILVEV++HIVTEVAV++P Sbjct: 161 LGQVLKSPIIASFGPQIPLKIEPQGAVKVQLDTRQQGAGINMILVEVFVHIVTEVAVVVP 220 Query: 282 FDMEPQVVDTEIPVSYLMVVGDVPMYYYDNQGQPVGDN 319 FDMEPQ+V+T+IP+SYL+VVGDVPMYYYD QG+ VG+N Sbjct: 221 FDMEPQIVETDIPISYLLVVGDVPMYYYDGQGKAVGEN 258 >B1DFC9.1 B1DFC9_9BACL Sporulation protein YunB Length = 291 Score = 287 bits (734), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 126/217 (58%), Positives = 177/217 (81%), Gaps = 1/217 (0%) Query: 104 RSQKPRRRGRFWLIFS-LVLILAVLQSLRYVEKHLKPPILHLAQIRVKQIATESINKAIT 162 R + +RR + W+I + L+L++ LQSL Y+EKHL+PP++ +AQ+R+KQ+AT++INKA T Sbjct: 14 RLKSGKRRTKLWIIVTVLILLIGTLQSLVYIEKHLRPPLMKVAQLRIKQVATQAINKATT 73 Query: 163 TQVADTGNAEALIDWKTDKEGKISGFMLNYKEHMRITSQAAEVIQSTLQELHNQTERIPL 222 +V + E LIDWK + +GK+SGFMLNY EH +I S+ + +Q+TL EL E IP+ Sbjct: 74 DEVVSNADFEKLIDWKMNSDGKVSGFMLNYAEHTKIRSETIKTVQATLDELKEIPEHIPV 133 Query: 223 GQALGSSLIASFGPDVPIKIEPQGAVKVELNTRQKDAGINMILVEVYIHIVTEVAVIIPF 282 G ALGS+LI+++GP +P+K EP GAVKV+LNTRQ +AGINMIL+EVYIHIV+EVA+I+PF Sbjct: 134 GNALGSALISTYGPRIPVKFEPVGAVKVDLNTRQTNAGINMILIEVYIHIVSEVAIIVPF 193 Query: 283 DMEPQVVDTEIPVSYLMVVGDVPMYYYDNQGQPVGDN 319 + +PQ+V+TEIP+SYL+VVGDVPMYYYDN+G+ VG++ Sbjct: 194 ETDPQIVETEIPISYLLVVGDVPMYYYDNEGKAVGES 230 >C0ZKX3.1 C0ZKX3_BREBN Putative uncharacterized protein yunB Length = 241 Score = 157 bits (397), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 76/216 (35%), Positives = 135/216 (62%), Gaps = 4/216 (1%) Query: 104 RSQKPRRRGRFWLIFSLVLILAVLQSLRYVEKHLKPPILHLAQIRVKQIATESINKAITT 163 R + P R R +LI ++ + +Q+L +++ L+P ++ LA + +Q+A E+I A+T Sbjct: 12 RWRNPLSRTRAFLIALVIFFVLTIQTLVFLQNRLEPTLIILATKKAEQLAKEAITDAVTK 71 Query: 164 QVADTG-NAEALIDWKTDKEGKISGFMLNYKEHMRITSQAAEVIQSTLQELHNQT--ERI 220 +++ G + ++ + + +G+I + N+KE+ RI + +Q+ LQE + I Sbjct: 72 RISQQGVDFNQIVKIEKNNKGQIQAYQFNFKEYARIVGETTARVQNKLQEFEQEKVDRTI 131 Query: 221 PLGQALGSSLIASFGPDVPIKIEPQGAVKVELNTRQKDAGINMILVEVYIHIVTEVAVII 280 PLG A G+S +AS GP++P+ P G+VK +L T K+AGINM+L VYI + ++ ++I Sbjct: 132 PLGLATGNSFLASMGPNLPVTFVPIGSVKTKLETELKEAGINMVLATVYIFVEVDLRIVI 191 Query: 281 PFDMEPQVVDTEIPVSYLMVVGDVPMYYYDN-QGQP 315 PF E Q V T+IP+++ +++GDVP Y Y+N +G+P Sbjct: 192 PFATEEQTVTTKIPITHSLIIGDVPTYLYNNSEGKP 227 >C0ZJS3.1 C0ZJS3_BREBN Putative uncharacterized protein yunB Length = 238 Score = 135 bits (340), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 75/187 (40%), Positives = 113/187 (60%), Gaps = 6/187 (3%) Query: 134 EKHLKPPILHLAQIRVKQIATESINKAITTQVADTG-NAEALIDWKTDKEGKISGFMLNY 192 E+ ++P ++ +AQ + +Q+A +I A+T ++ G + ++ + D+ G I N+ Sbjct: 34 ERRVEPTLMLIAQAKAEQVAKLAITDAVTKRMTQQGVDVNEVVIMEKDESGSIKAVNFNF 93 Query: 193 KEHMRITSQAAEVIQSTLQEL---HNQTERIPLGQALGSSLIASFGPDVPIKIEPQGAVK 249 KE+ RI + IQ+ L+E H QT IPLG A + + GPD+P+ P GAVK Sbjct: 94 KEYSRIVGETTARIQNRLKEFEQEHVQT-TIPLGLATKNVFLEHIGPDIPVSFVPIGAVK 152 Query: 250 VELNTRQKDAGINMILVEVYIHIVTEVAVIIPFDMEPQVVDTEIPVSYLMVVGDVPMYYY 309 +L T+ K AGINM+LV VYI++ ++ VIIPF + Q V TEIPVS ++VG VP Y Y Sbjct: 153 TKLETKLKQAGINMVLVTVYIYVEVDLRVIIPFATKQQTVTTEIPVSEALIVGKVPTYLY 212 Query: 310 DN-QGQP 315 DN G+P Sbjct: 213 DNPSGKP 219 >C0GJL1.1 C0GJL1_9FIRM Sporulation protein YunB Length = 230 Score = 121 bits (303), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 71/195 (36%), Positives = 109/195 (55%) Query: 111 RGRFWLIFSLVLILAVLQSLRYVEKHLKPPILHLAQIRVKQIATESINKAITTQVADTGN 170 R F V I +++ Y +++L+P IL +A R IATE+IN A++ +VA Sbjct: 10 REAFIFFLIAVFIFGIIRMFLYFDRNLRPTILSVASARADIIATEAINSAVSEKVARNIL 69 Query: 171 AEALIDWKTDKEGKISGFMLNYKEHMRITSQAAEVIQSTLQELHNQTERIPLGQALGSSL 230 + LI + D+EG+I N E R+ S+ +Q TL L + IPLGQALGS L Sbjct: 70 YQDLILLEKDREGRIVMAQTNNMEVNRLMSETTMRVQETLTSLKGEKIYIPLGQALGSYL 129 Query: 231 IASFGPDVPIKIEPQGAVKVELNTRQKDAGINMILVEVYIHIVTEVAVIIPFDMEPQVVD 290 +A+ GP +PI + P G V E+ + ++AGIN + ++Y+ I EV +IIPF + V Sbjct: 130 LANVGPRIPITLIPIGFVNTEIIDQFEEAGINQVRHKIYLDIHAEVEIIIPFVSQVTKVS 189 Query: 291 TEIPVSYLMVVGDVP 305 T +P+ +G+VP Sbjct: 190 TTVPIVDANYIGEVP 204 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,908,318,395 Number of extensions: 70507325 Number of successful extensions: 174619 Number of sequences better than 1.0e-05: 155 Number of HSP's gapped: 174519 Number of HSP's successfully gapped: 155 Length of query: 385 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 248 Effective length of database: 1,770,017,148 Effective search space: 438964252704 Effective search space used: 438964252704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)