BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0021.p (228 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8VVT9.1 A8VVT9_9BACI L-seryl-tRNA(Sec) selenium transferase EC=... 95 5e-18 Q6NMJ6.1 Q6NMJ6_ARATH At1g03260 57 1e-06 C1XIU3.1 C1XIU3_MEIRU Uncharacterized conserved protein 56 2e-06 C3J2P8.1 C3J2P8_9BACI SNARE associated Golgi protein 55 4e-06 B7YBE6.1 B7YBE6_9BACI SNARE associated Golgi protein 55 4e-06 >A8VVT9.1 A8VVT9_9BACI L-seryl-tRNA(Sec) selenium transferase EC=2.9.1.1 Length = 226 Score = 94.7 bits (234), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 60/191 (31%), Positives = 98/191 (51%), Gaps = 5/191 (2%) Query: 39 QSLGLSGPYILLVTIPLTVLQSLFGFFPLAVLIVLHVSVFNVIGGMLVSWLACNVGAMLS 98 + G G ILL TIPL ++Q++ FP+ +LIV H VF V GG+L S+ +GA++ Sbjct: 38 EMFGQWGYGILLFTIPLMMVQNVVTLFPILILIVFHFVVFGVWGGILASFAGTFLGAVVC 97 Query: 99 YXXXXXXXXXXXXXXXXSKLKRYDKWQRYLDRYGVWTLVLLRTVPIMPSNVINFMSAVSP 158 + R K +D YGV+ LVLLR++P+MPSN+I+ +A SP Sbjct: 98 FFLARSAAGRFFDRYWEKNEDRLKKVIHAIDHYGVYMLVLLRSIPVMPSNLISIAAAASP 157 Query: 159 MKVPAFIWGTVLGNLSYIWLFGTLGSTLIVSREEWN-GFLLWYXXXXXXXXXXXXRRHWG 217 + +++W TV GN++ I + L L V G+++++ R Sbjct: 158 ISTVSYMWSTVFGNIAMIGVLSFLSMPLWVDESGAGPGYIVFFSLFFMLLIGYFLR---- 213 Query: 218 HLQEDKRRRME 228 HL E+++ R++ Sbjct: 214 HLYEERKVRVK 224 >Q6NMJ6.1 Q6NMJ6_ARATH At1g03260 Length = 274 Score = 57.4 bits (137), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 46/170 (27%), Positives = 83/170 (48%), Gaps = 21/170 (12%) Query: 25 PMTETINAWIDWLLQSLGLSGPYIL-LVTIPLTVLQSLFGFFPLAVLIVLHVSVFNVIGG 83 P+ + + ++ W+ + LG GP L L IPLT+ + + SV + GG Sbjct: 31 PVEQKLKDFLLWIKEDLGPFGPLALALAYIPLTI-------------VAVPASVLTLGGG 77 Query: 84 ML----VSWLACNVGAMLSYXXXXXXXXXXXXXXXXSKLKRYDKWQRY---LDRYGVWTL 136 L V ++A ++GA L SK+K Y K+Q + + G + Sbjct: 78 YLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPKFQAVSVAIQKSGFKIV 137 Query: 137 VLLRTVPIMPSNVINFMSAVSPMKVPAFIWGTVLGNLSYIWLFGTLGSTL 186 +LLR VPI+P N++N++ +V+P+++ ++ T LG + + +G+TL Sbjct: 138 LLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALVYVGTTL 187 >C1XIU3.1 C1XIU3_MEIRU Uncharacterized conserved protein Length = 235 Score = 56.2 bits (134), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 39/157 (24%), Positives = 75/157 (47%), Gaps = 9/157 (5%) Query: 28 ETINAWIDWLLQSLGLSGPYILLVTIPLTVLQSLFGFFPLAVLIVLHVSVFNVIGGMLVS 87 + I AW+ LG GP L I L + Q+L P+++++++ V F + G ++ Sbjct: 53 QAIQAWV----AGLGWLGP---LSIIGLMIAQTLVSVVPMSLVMLISVLAFGPVFGGILG 105 Query: 88 WLACNVGAMLSYXXXXXXXXXXXXXXXXSKLKRYDKWQRYLDRYGVWTLVLLRTVPIMPS 147 W+ + AM+ Y +++R K + ++RYG W ++ LR ++P+ Sbjct: 106 WIGAVIAAMVGYSLARLLGPVVVDRFVSEEIRR--KVEAQVERYGPWAIIALRLSFVVPT 163 Query: 148 NVINFMSAVSPMKVPAFIWGTVLGNLSYIWLFGTLGS 184 + +NF++ + M F+ T LG L + LG+ Sbjct: 164 DSVNFVAGLVRMHPLKFLLATALGALPVAVVIAWLGA 200 >C3J2P8.1 C3J2P8_9BACI SNARE associated Golgi protein Length = 207 Score = 55.1 bits (131), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 39/163 (23%), Positives = 77/163 (47%), Gaps = 7/163 (4%) Query: 28 ETINAW--IDWLLQSLGLSGPYILLVTIPLTVLQSLFGFFPLAVLIVLHVSVFNVIGGML 85 ETI W +D +L L + +L I T+L+S F P+ V ++ + + F + G L Sbjct: 4 ETIKQWFTLDSILSLLEQYRSFGVLPGIAATLLESFFPILPMFVFVMANAAAFGLWKGFL 63 Query: 86 VSWLACNVGAMLSY--XXXXXXXXXXXXXXXXSKLKRYDKWQRYLDRYGVWTLVLLRTVP 143 +SWL ++G+++ + K++++ W ++R+G L LL P Sbjct: 64 ISWLGASLGSLIVFWLTRKVGQKRFFHFVRRHPKVRQFMHW---IERHGFGPLFLLYCFP 120 Query: 144 IMPSNVINFMSAVSPMKVPAFIWGTVLGNLSYIWLFGTLGSTL 186 PS ++N ++ +S + F+ +LG + ++ +G L Sbjct: 121 FTPSALVNIVAGLSRISRQQFVLAVLLGKMIMVFTISFIGYDL 163 >B7YBE6.1 B7YBE6_9BACI SNARE associated Golgi protein Length = 207 Score = 55.1 bits (131), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 39/163 (23%), Positives = 77/163 (47%), Gaps = 7/163 (4%) Query: 28 ETINAW--IDWLLQSLGLSGPYILLVTIPLTVLQSLFGFFPLAVLIVLHVSVFNVIGGML 85 ETI W +D +L L + +L I T+L+S F P+ V ++ + + F + G L Sbjct: 4 ETIKQWFTLDSILSLLEQYRSFGVLPGIAATLLESFFPILPMFVFVMANAAAFGLWKGFL 63 Query: 86 VSWLACNVGAMLSY--XXXXXXXXXXXXXXXXSKLKRYDKWQRYLDRYGVWTLVLLRTVP 143 +SWL ++G+++ + K++++ W ++R+G L LL P Sbjct: 64 ISWLGASLGSLIVFWLTRKVGQKRFFHFVRRHPKVRQFMHW---IERHGFGPLFLLYCFP 120 Query: 144 IMPSNVINFMSAVSPMKVPAFIWGTVLGNLSYIWLFGTLGSTL 186 PS ++N ++ +S + F+ +LG + ++ +G L Sbjct: 121 FTPSALVNIVAGLSRISRQQFVLAVLLGKMIMVFTISFIGYDL 163 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.476 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,272,990,228 Number of extensions: 46060889 Number of successful extensions: 158216 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 158618 Number of HSP's successfully gapped: 65 Length of query: 228 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 97 Effective length of database: 1,826,543,238 Effective search space: 177174694086 Effective search space used: 177174694086 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)