BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0019.p (277 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DDZ6.1 B1DDZ6_9BACL Binding-protein-dependent transport system... 260 8e-68 B3KE20.1 B3KE20_9BACI Binding-protein-dependent transport system... 217 8e-55 C0Z5E1.1 C0Z5E1_BREBN Glycine betaine ABC transporter permease p... 207 8e-52 A7Z146.1 A7Z146_BACA2 OpuAB 198 5e-49 P46921.1 OPUAB_BACSU Glycine betaine transport system permease p... 197 9e-49 >B1DDZ6.1 B1DDZ6_9BACL Binding-protein-dependent transport systems inner membrane component Length = 278 Score = 260 bits (665), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 138/275 (50%), Positives = 166/275 (60%) Query: 2 NIPKLPLGKAVEWLENWLTTYLGPFFDFIHAVIGGMVNGIEXXXXXXXXXXXXXXXXXXX 61 NIPKLPLG +E LE+WL + P FDFI +IG +V+ + Sbjct: 3 NIPKLPLGNWIESLESWLENHFTPLFDFIRLIIGSIVDFLHNVLLFSPSILMILIITALA 62 Query: 62 YWIGKWRMXXXXXXXXXXXXXXXXXXPSMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQ 121 +++GKW++ +M+ IGVP G+LCA+S+ +Q Sbjct: 63 WFLGKWKVAAFALIGLLLIDNLGYWEHAMETLSIVLTASLLSIIIGVPTGILCARSSFLQ 122 Query: 122 NTVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPPTIRLTNLGIRQVSPEL 181 +TP+LD MQTMPAFVYLLPAVSFFSLGVVPGVIASIIFA+PPTIRLTNLGIRQV EL Sbjct: 123 GIITPLLDLMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAVPPTIRLTNLGIRQVPVEL 182 Query: 182 VEAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRA 241 VEAADAFGST QKLFKLQLP+ALPT+MAGINQT GAQG+GA VYRA Sbjct: 183 VEAADAFGSTPNQKLFKLQLPMALPTMMAGINQTIMLSLSMVVISSMIGAQGLGADVYRA 242 Query: 242 VSQAKTXXXXXXXXXXXXXXXLLDRLTQKLVKPKK 276 V+Q KT LLDR++ +VK K Sbjct: 243 VTQTKTGIGFEAGISIVVLAILLDRMSNHIVKKNK 277 >B3KE20.1 B3KE20_9BACI Binding-protein-dependent transport systems inner membrane component Length = 277 Score = 217 bits (553), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 123/274 (44%), Positives = 148/274 (54%) Query: 3 IPKLPLGKAVEWLENWLTTYLGPFFDFIHAVIGGMVNGIEXXXXXXXXXXXXXXXXXXXY 62 +PK+PL V+ L +W+ FFD I +I +V Y Sbjct: 1 MPKIPLASWVDSLVDWMGDVFSGFFDAISFIIENVVQLFTDLFMLPHPYLFIVILAVIAY 60 Query: 63 WIGKWRMXXXXXXXXXXXXXXXXXXPSMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQN 122 +G+ + +M +GVPLG+ A S Sbjct: 61 LLGRLPLTLFTAIGFLLIDNLGYWSQTMSTLSLVITAALISVILGVPLGIWAAYSKSASR 120 Query: 123 TVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPPTIRLTNLGIRQVSPELV 182 + PVLDFMQTMPAFVYLLPAV+FFSLGVVPGVIAS+IFAIPPTIRLT LGI QVS EL Sbjct: 121 VIIPVLDFMQTMPAFVYLLPAVTFFSLGVVPGVIASVIFAIPPTIRLTRLGIMQVSGELT 180 Query: 183 EAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRAV 242 EAADAFGSTSGQKLFK+QLP+A+PT+MAGINQT GAQG+GA VYRAV Sbjct: 181 EAADAFGSTSGQKLFKVQLPLAMPTLMAGINQTIMLSLSMVVIASMIGAQGIGAEVYRAV 240 Query: 243 SQAKTXXXXXXXXXXXXXXXLLDRLTQKLVKPKK 276 +Q + +LDR TQ + K KK Sbjct: 241 TQLQIGKGFEAGLAVVILAIVLDRFTQNIFKSKK 274 >C0Z5E1.1 C0Z5E1_BREBN Glycine betaine ABC transporter permease protein Length = 281 Score = 207 bits (527), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 123/274 (44%), Positives = 150/274 (54%) Query: 4 PKLPLGKAVEWLENWLTTYLGPFFDFIHAVIGGMVNGIEXXXXXXXXXXXXXXXXXXXYW 63 PKLPL VE + + L LG FDFI A IGG V+ Y Sbjct: 8 PKLPLDSWVETIVDGLEKNLGFLFDFISAGIGGTVDLFSWILTGIPFWALIILFTLLAYR 67 Query: 64 IGKWRMXXXXXXXXXXXXXXXXXXPSMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQNT 123 GK + SM+ IG+P+G+ CA+++ VQ Sbjct: 68 AGKVAIALFTFIGLLLIQNLGYWEHSMETLALVLTAGLISVIIGIPVGIWCAKNDSVQKV 127 Query: 124 VTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPPTIRLTNLGIRQVSPELVE 183 VTP+LDFMQTMPAFVYL+PA+ FF LG VPGVIAS+IFA+PPTIRLTNLGIRQV +L+E Sbjct: 128 VTPLLDFMQTMPAFVYLIPAIFFFGLGKVPGVIASVIFAMPPTIRLTNLGIRQVPADLIE 187 Query: 184 AADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRAVS 243 A++AFGST QKL K+QLPIA T+MAGINQ+ GA+G+GA VYRAV+ Sbjct: 188 ASEAFGSTYWQKLTKVQLPIAKTTMMAGINQSIMLALSMVVIASMIGAKGLGADVYRAVT 247 Query: 244 QAKTXXXXXXXXXXXXXXXLLDRLTQKLVKPKKG 277 Q K LLDRLTQ + K K Sbjct: 248 QIKIGEGFEAGLAIVILAILLDRLTQSVGKRKNA 281 >A7Z146.1 A7Z146_BACA2 OpuAB Length = 282 Score = 198 bits (503), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 109/275 (39%), Positives = 145/275 (52%) Query: 2 NIPKLPLGKAVEWLENWLTTYLGPFFDFIHAVIGGMVNGIEXXXXXXXXXXXXXXXXXXX 61 +P++PL V+ +WLT G FFD I + VN I Sbjct: 3 RLPRIPLADIVDRFVDWLTMTFGGFFDGISNGLAAFVNAIVSGLGFIPSILLTVIFAALA 62 Query: 62 YWIGKWRMXXXXXXXXXXXXXXXXXXPSMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQ 121 +WI + P +Q IGVP+G+ +Q V+ Sbjct: 63 WWISTRGIALFTLIGFLVIDYLGYWSPMLQTLALVVTSVLISIIIGVPIGIWASQKETVR 122 Query: 122 NTVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPPTIRLTNLGIRQVSPEL 181 VTP+LD MQTMPAFVYLLPA+ FF++GVVPGV+AS+IFA+PPTIR+T LGI+QV +L Sbjct: 123 RIVTPILDLMQTMPAFVYLLPAIFFFNIGVVPGVVASVIFAMPPTIRMTVLGIKQVPADL 182 Query: 182 VEAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRA 241 +EA +AFGST+ Q+LFK+QLP+A TI+AGINQ+ GA G+G+ VY A Sbjct: 183 IEATEAFGSTTSQRLFKVQLPLATKTILAGINQSIMLALSMVVIAAMVGAPGLGSEVYSA 242 Query: 242 VSQAKTXXXXXXXXXXXXXXXLLDRLTQKLVKPKK 276 V+Q KT LDR+TQ + KK Sbjct: 243 VTQLKTGVGVEAGIAIVIVAITLDRITQNIKVKKK 277 >P46921.1 OPUAB_BACSU Glycine betaine transport system permease protein opuAB Length = 282 Score = 197 bits (501), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 107/275 (38%), Positives = 146/275 (53%) Query: 2 NIPKLPLGKAVEWLENWLTTYLGPFFDFIHAVIGGMVNGIEXXXXXXXXXXXXXXXXXXX 61 +P++PL ++ +W+T G FFD I + VNGI Sbjct: 3 RLPRIPLADIIDRFVDWITMTFGGFFDGIANGLAAFVNGIVTGLGFIPSILLTIIFAALA 62 Query: 62 YWIGKWRMXXXXXXXXXXXXXXXXXXPSMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQ 121 +WI + P +Q +GVP+G+ +Q V+ Sbjct: 63 WWISTRGIALFTLIGFLLIDYLGYWDPMLQTLALVLTSVIISIVVGVPIGIWASQKETVR 122 Query: 122 NTVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPPTIRLTNLGIRQVSPEL 181 VTP+LD MQTMPAFVYLLPA+ FF++GVVPGV+AS+IFA+PPTIR+T LGI+QV +L Sbjct: 123 RIVTPILDLMQTMPAFVYLLPAIFFFNIGVVPGVVASVIFAMPPTIRMTVLGIKQVPADL 182 Query: 182 VEAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRA 241 +EA +AFGST+ Q+LFK+QLP+A TI+AGINQ+ GA G+G+ VY A Sbjct: 183 IEATEAFGSTTAQRLFKVQLPLATKTILAGINQSIMLALSMVVIAAMVGAPGLGSEVYSA 242 Query: 242 VSQAKTXXXXXXXXXXXXXXXLLDRLTQKLVKPKK 276 V+Q KT LDR+TQ + KK Sbjct: 243 VTQLKTGVGVEAGIAIVIVAITLDRITQNIKVKKK 277 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,139,913,728 Number of extensions: 35433688 Number of successful extensions: 100924 Number of sequences better than 1.0e-05: 2375 Number of HSP's gapped: 101362 Number of HSP's successfully gapped: 2423 Length of query: 277 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 143 Effective length of database: 1,798,280,193 Effective search space: 257154067599 Effective search space used: 257154067599 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)