BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0015.p (340 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB81.1 B3KB81_9BACI Periplasmic binding protein/LacI transcrip... 508 e-142 B7GM29.1 B7GM29_ANOFW Transcriptional regulator, LacI family (HT... 414 e-114 Q2BEB0.1 Q2BEB0_9BACI Transcriptional regulator, LacI family pro... 399 e-109 A8VQG1.1 A8VQG1_9BACI ABC-2 type transporter 396 e-108 Q5WCJ5.1 Q5WCJ5_BACSK LacI family transcriptional regulator 379 e-103 >B3KB81.1 B3KB81_9BACI Periplasmic binding protein/LacI transcriptional regulator Length = 346 Score = 508 bits (1307), Expect = e-142, Method: Compositional matrix adjust. Identities = 239/337 (70%), Positives = 294/337 (87%), Gaps = 1/337 (0%) Query: 1 MQLNIKKIAEMAGVSVSTVSKIINNYSDVSEKTKKRVMEIIEQTGYSPSNSAKTLATKKS 60 M LNIK IA+MAGVSVSTVSKIINNYSDVS++TK RV+EI+ +TGY+PSNSAKTLATKKS Sbjct: 1 MTLNIKTIAKMAGVSVSTVSKIINNYSDVSDETKARVLEIMRETGYTPSNSAKTLATKKS 60 Query: 61 SLIGVIFAGELNVEFTHPFFVEVLNAFKKQMGVLGYDLIFFSNEKFI-SSGDYYSRCLHF 119 +LIGVIFAG+LNV+FTHPFFVEVLN+FKKQMGVLGYDL+FFSNEKF DY RC H+ Sbjct: 61 NLIGVIFAGKLNVDFTHPFFVEVLNSFKKQMGVLGYDLLFFSNEKFQGEDADYLGRCRHY 120 Query: 120 NVDGCVIISGQKMEPAIRDLDMSSIPCIGVDLELRGKKSGYVMSDNVQIASKVVEHFYLL 179 VDGCVIISG+++EP+I++LD S IPCIGVD+EL G+ SG++MSDN +++SKVVEH+YL Sbjct: 121 QVDGCVIISGEEIEPSIKELDESDIPCIGVDIELFGEMSGHIMSDNYKMSSKVVEHYYLQ 180 Query: 180 GHRELGFIGSTADSDISNQREAGFVKAVESFGLTLNRNWFVHGDDFFESSGYTAMRRLLE 239 G R+LGFIG + +S+ISN REAG+ A+ESFGL WF++GDDF+E+SGY AM RL++ Sbjct: 181 GFRDLGFIGGSKESEISNMREAGYRNAMESFGLPARAEWFLNGDDFYEASGYKAMTRLIK 240 Query: 240 SGSMPQAVFAASDLLALGAIRALKEQGLRIPEDVAIIGCDDIEACKYTSPTLTTIRQNKE 299 +GS+P+A+FAASD +A+GAI+AL+E GL +P+D++IIGCDDIEACKYT+P LTTIRQNKE Sbjct: 241 TGSLPRAIFAASDQIAIGAIQALQEHGLNVPDDMSIIGCDDIEACKYTTPKLTTIRQNKE 300 Query: 300 RLGVLAAHMLYDLINNQSDGGSFVVEPALIVRESCGS 336 ++G LAA MLYDLI+ QS SFVVEP LIVRESCGS Sbjct: 301 KIGRLAALMLYDLIHKQSPVSSFVVEPELIVRESCGS 337 >B7GM29.1 B7GM29_ANOFW Transcriptional regulator, LacI family (HTH and periplasmic-binding domains) Length = 342 Score = 414 bits (1064), Expect = e-114, Method: Compositional matrix adjust. Identities = 190/337 (56%), Positives = 258/337 (76%), Gaps = 3/337 (0%) Query: 1 MQLNIKKIAEMAGVSVSTVSKIINNYSDVSEKTKKRVMEIIEQTGYSPSNSAKTLATKKS 60 M++ IK+IA +AGVS TVSK++NNY DV E+TKKRV+EI+E+TGY + K A KS Sbjct: 1 MKITIKEIARLAGVSPGTVSKVLNNYHDVGEETKKRVLEIMEKTGYVAARIQKDTA-HKS 59 Query: 61 SLIGVIFAGELNVEFTHPFFVEVLNAFKKQMGVLGYDLIFFSNEKFIS-SGDYYSRCLHF 119 ++GVI+AGE+ +F HPFF+EV+N+FKK MG GYDL+FFSNE+F DYY R + + Sbjct: 60 RVVGVIYAGEIYADFNHPFFIEVMNSFKKTMGTFGYDLLFFSNERFEEGEEDYYERAMRY 119 Query: 120 NVDGCVIISGQKMEPAIRDLDMSSIPCIGVDLELRGKKSGYVMSDNVQIASKVVEHFYLL 179 VDG +IISG+K++P+I LDMS IPCIGVD+ L G +S Y+M+DN +I++KVVEHFYL Sbjct: 120 KVDGVIIISGEKVQPSIYRLDMSDIPCIGVDIPLEGNRSAYIMTDNYKISAKVVEHFYLC 179 Query: 180 GHRELGFIGSTADSDISNQREAGFVKAVESFGLTLNRNWFVHGDDFFESSGYTAMRRLLE 239 G+R +G+IG + ++ RE GF ++ FGLT++ WFV GD FF SGY AM+++L Sbjct: 180 GYRHIGYIGGLPGAQVTKIREQGFRNTMKEFGLTIHEEWFVQGD-FFMESGYEAMKKILL 238 Query: 240 SGSMPQAVFAASDLLALGAIRALKEQGLRIPEDVAIIGCDDIEACKYTSPTLTTIRQNKE 299 S P+AVFAASDL+A GA++A+KE GL+IPED+AI+GCDDI+AC YT P LT++RQ+KE Sbjct: 239 SSHHPEAVFAASDLMAFGAMKAVKEHGLKIPEDIAIVGCDDIDACNYTDPPLTSVRQDKE 298 Query: 300 RLGVLAAHMLYDLINNQSDGGSFVVEPALIVRESCGS 336 ++G LAA+ML D+I+ Q + +VEP LI+RESCGS Sbjct: 299 KIGRLAAYMLRDIIHRQFQPKAVMVEPELIIRESCGS 335 >Q2BEB0.1 Q2BEB0_9BACI Transcriptional regulator, LacI family protein Length = 345 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 182/339 (53%), Positives = 260/339 (76%), Gaps = 2/339 (0%) Query: 1 MQLNIKKIAEMAGVSVSTVSKIINNYSDVSEKTKKRVMEIIEQTGYSPSNSAKTLATKKS 60 M L IK IAE+AGVS +TVSK+INNY +SE+TKK+VM++I + Y P+ SA++LATKKS Sbjct: 8 MSLTIKNIAEIAGVSAATVSKVINNYGGISERTKKKVMDVILEMDYEPNFSARSLATKKS 67 Query: 61 SLIGVIFAGELNVEFTHPFFVEVLNAFKKQMGVLGYDLIFFSNEKFIS-SGDYYSRCLHF 119 +LIG+I+AG++NV+ THP+F EV+++FKK +G+LGYD++ FSNE F +G Y +RC HF Sbjct: 68 NLIGLIYAGKINVDMTHPYFNEVISSFKKNIGMLGYDILMFSNENFRQDNGSYMARCRHF 127 Query: 120 NVDGCVIISGQKMEPAIRDLDMSSIPCIGVDLELRGKKSGYVMSDNVQIASKVVEHFYLL 179 NVDGC++I+G+++E A+ +L + PCIG+D+EL G KS +VM+DNV ++ KV+EH YL Sbjct: 128 NVDGCLVIAGEEVEEAVHELAKADFPCIGIDIELSGPKSAFVMTDNVNLSRKVIEHLYLN 187 Query: 180 GHRELGFIGSTADSDISNQREAGFVKAVESFGLTLNRNWFVHGDDFFESSGYTAMRRLLE 239 R++GFIG S ISN R+ GF++A+ FGL + +W +G D+ E SGY M+R+LE Sbjct: 188 SVRDVGFIGGMKGSVISNLRKKGFLEAMNQFGLEIRDDWIQYG-DYHEDSGYDCMKRILE 246 Query: 240 SGSMPQAVFAASDLLALGAIRALKEQGLRIPEDVAIIGCDDIEACKYTSPTLTTIRQNKE 299 P+AVFAASDL+ALGA+RA+KE GL +P D+ +IGCDDI AC+Y+ P L+T++Q+KE Sbjct: 247 GKRKPEAVFAASDLMALGALRAIKEAGLSVPHDIRVIGCDDIAACRYSDPKLSTVKQDKE 306 Query: 300 RLGVLAAHMLYDLINNQSDGGSFVVEPALIVRESCGSKL 338 + G L+A+ML DLIN +S +++ LI+RESCG+ L Sbjct: 307 KFGKLSAYMLNDLINGKSQPRPALIDSELIIRESCGTHL 345 >A8VQG1.1 A8VQG1_9BACI ABC-2 type transporter Length = 342 Score = 396 bits (1017), Expect = e-108, Method: Compositional matrix adjust. Identities = 190/341 (55%), Positives = 257/341 (75%), Gaps = 3/341 (0%) Query: 1 MQLNIKKIAEMAGVSVSTVSKIINNYSDVSEKTKKRVMEIIEQTGYSPSNSAKTLATKKS 60 M++ IK+IA++AGVS +TVSKIINNY DV +KTK+RV+ I+++ GY PS +A++L K + Sbjct: 1 MKMTIKEIAKLAGVSQATVSKIINNYDDVGQKTKERVLAIMDEYGYRPSYAAQSLVKKVT 60 Query: 61 SLIGVIFAGELNVEFTHPFFVEVLNAFKKQMGVLGYDLIFFSNEKFISSGD-YYSRCLHF 119 ++GVI+AG +N F HPFFVEV++ FKK +G GYDL+FFSNEKF + + Y RC H+ Sbjct: 61 KVVGVIYAGHINANFNHPFFVEVVDMFKKTLGGEGYDLLFFSNEKFHKADENYLQRCQHY 120 Query: 120 NVDGCVIISGQKMEPAIRDLDMSSIPCIGVDLELRGKKSGYVMSDNVQIASKVVEHFYLL 179 NVDGC+II G+++EP+I +LD S+IPCIGVD+EL G SGY+M+DN+Q++S VEHFYL Sbjct: 121 NVDGCIIIGGEQVEPSIYELDQSTIPCIGVDIELTGPHSGYIMTDNIQVSSMAVEHFYLT 180 Query: 180 GHRELGFIGSTADSDISNQREAGFVKAVESFGLTLNRNWFVHGDDFFESSGYTAMRRLLE 239 G RE+ FIG +S +S QREAGF + ++++GL WFV+G DFFE SGY M+ LL Sbjct: 181 GRREVAFIGGEKESFVSRQREAGFRERIKAYGLQAPEEWFVYG-DFFEESGYACMKELLR 239 Query: 240 SGSMPQAVFAASDLLALGAIRALKEQGLRIPEDVAIIGCDDIEACKYTSPTLTTIRQNKE 299 S PQA+FA SDL+A GA++A+K+QGL I D AIIGCDDI A K+ P LTTI+Q+K+ Sbjct: 240 SERTPQAIFAISDLMAFGAMKAIKDQGLDI-HDFAIIGCDDITAAKHVEPPLTTIKQDKQ 298 Query: 300 RLGVLAAHMLYDLINNQSDGGSFVVEPALIVRESCGSKLTG 340 ++G +AA +L DLI + S V+P L+VRESCGSK G Sbjct: 299 KIGRMAAFILKDLITGHIEQTSLKVDPELVVRESCGSKKPG 339 >Q5WCJ5.1 Q5WCJ5_BACSK LacI family transcriptional regulator Length = 343 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 177/337 (52%), Positives = 247/337 (73%), Gaps = 2/337 (0%) Query: 3 LNIKKIAEMAGVSVSTVSKIINNYSDVSEKTKKRVMEIIEQTGYSPSNSAKTLATKKSSL 62 + IK IA+ AGVS +T+SKI+N+Y DVS+KTK+RV I+E+ GY PS+SA+TLA K S L Sbjct: 7 ITIKDIAKKAGVSQATISKILNDYPDVSQKTKERVFRIMEEAGYRPSHSARTLAQKTSKL 66 Query: 63 IGVIFAGELNVEFTHPFFVEVLNAFKKQMGVLGYDLIFFSNEKFISSGDYYSRCLHFNVD 122 IGV++AG +N +F HPFF+EVLNAFK+ +G+ GYDL+FFS+E F ++Y+RCL++ VD Sbjct: 67 IGVVYAGRINADFDHPFFIEVLNAFKRTIGMHGYDLLFFSDETFQKEENFYARCLYYKVD 126 Query: 123 GCVIISGQKMEPAIRDLDMSSIPCIGVDLELRGKKSGYVMSDNVQIASKVVEHFYLLGHR 182 GC+II+G+ ++ +I DL S IPCIGVD++L G KSGYVM+DN +I+ VV++FYL GHR Sbjct: 127 GCIIIAGEDVQQSIEDLTKSEIPCIGVDIQLDGPKSGYVMTDNKKISQSVVDYFYLQGHR 186 Query: 183 ELGFIGSTADSDISNQREAGFVKAVESFGLTLNRNWFVHGDDFFESSGYTAMRRLLESGS 242 ++ +IG +S +SN R GF +++ +GLTL W + G DFFE SGY A L + + Sbjct: 187 DIAYIGGANESAVSNIRLKGFRESIHEYGLTLRPEWVIEG-DFFEQSGYEAGLSLFKGNA 245 Query: 243 M-PQAVFAASDLLALGAIRALKEQGLRIPEDVAIIGCDDIEACKYTSPTLTTIRQNKERL 301 P AVF++SDL+A G +RA++E G+ IP DV+++GCDD A +Y P LTT+RQNKE++ Sbjct: 246 QKPTAVFSSSDLMAFGFMRAMQELGVSIPGDVSLVGCDDQLAARYVQPPLTTVRQNKEKI 305 Query: 302 GVLAAHMLYDLINNQSDGGSFVVEPALIVRESCGSKL 338 G LAA ML D I ++ +VEP LI RE+ S L Sbjct: 306 GRLAALMLLDFIAGRASTARILVEPELIGRETTASLL 342 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,885,502,482 Number of extensions: 76006602 Number of successful extensions: 223702 Number of sequences better than 1.0e-05: 9194 Number of HSP's gapped: 208915 Number of HSP's successfully gapped: 9380 Length of query: 340 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 204 Effective length of database: 1,779,438,163 Effective search space: 363005385252 Effective search space used: 363005385252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)