BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0007.p (233 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIJ4.1 B3KIJ4_9BACI Glycosyl hydrolase family 88 290 6e-77 O34559.1 URHG2_BACSU Unsaturated rhamnogalacturonyl hydrolase yt... 265 2e-69 B4ANQ9.1 B4ANQ9_BACPU Glycosyl hydrolase, family 88 EC=3.2.1.- 258 4e-67 A8FGF0.1 A8FGF0_BACP2 Unsaturated glucuronyl hydrolase 257 5e-67 Q65FY8.1 Q65FY8_BACLD YteR 254 6e-66 >B3KIJ4.1 B3KIJ4_9BACI Glycosyl hydrolase family 88 Length = 370 Score = 290 bits (742), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 134/224 (59%), Positives = 168/224 (75%) Query: 1 MWLDGLYMAGVFSLKYANTYGETSLRETVLHQERLMRRHMKDEATGLLYHAWDESKRMPW 60 MWLDGLYM GVF++ YA Y E L + VL QE+LMR H KDEATGL YHAWDES+ MPW Sbjct: 142 MWLDGLYMGGVFTMNYARQYNEPHLFDEVLFQEKLMRSHTKDEATGLYYHAWDESRTMPW 201 Query: 61 ANPRTGCSPEFWSRSLGWYGLAVSQFLDLLPAGDPGRAELAAELSGFVEALIRYQDRESG 120 A+P +G SPEFW R++GWY L++ +FLDLLP R ELAA L EAL+RYQD +G Sbjct: 202 ADPVSGQSPEFWGRAIGWYALSLVEFLDLLPEHHSAREELAAALRELSEALVRYQDPATG 261 Query: 121 LWYQVVDKGDQPDNWLETSGSCLFVYTIAKAVKHGIAPPGALAAARKGYEGLIRVLQQDE 180 LW+QVVDKG + DNWLETS S LF YT+AK V+ G P + AR+GY+GL++ + +++ Sbjct: 262 LWHQVVDKGGREDNWLETSCSSLFTYTLAKGVRLGYLDPSFMTYARQGYQGLVQSVYRND 321 Query: 181 QGRLILPDICIGTSAGDYENYVTRPKVSNDLHGVGALVMACVEM 224 +G L++P ICIGT GDY++YV R + NDLHGVGALVMACVE+ Sbjct: 322 KGHLVMPQICIGTGVGDYKHYVERQQCENDLHGVGALVMACVEL 365 >O34559.1 URHG2_BACSU Unsaturated rhamnogalacturonyl hydrolase yteR EC=3.2.1.- Length = 373 Score = 265 bits (677), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 120/232 (51%), Positives = 161/232 (69%), Gaps = 1/232 (0%) Query: 1 MWLDGLYMAGVFSLKYANTYGETSLRETVLHQERLMRRHMKDEATGLLYHAWDESKRMPW 60 MWLDGLYM G F+LKYAN ET L + V+ QE LMR+H KD TGL YHAWDE+K+MPW Sbjct: 140 MWLDGLYMGGPFALKYANLKQETELFDQVVLQESLMRKHTKDAKTGLFYHAWDEAKKMPW 199 Query: 61 ANPRTGCSPEFWSRSLGWYGLAVSQFLDLLPAGDPGRAELAAELSGFVEALIRYQDRESG 120 AN TGCSPEFW+RS+GWY ++++ ++ LP P R L ++++ RYQD+E+G Sbjct: 200 ANEETGCSPEFWARSIGWYVMSLADMIEELPKKHPNRHVWKNTLQDMIKSICRYQDKETG 259 Query: 121 LWYQVVDKGDQPDNWLETSGSCLFVYTIAKAVKHGIAPPGALAAARKGYEGLIR-VLQQD 179 LWYQ+VDKGD+ DNWLE+SGSCL++Y IAK + G K Y+GLI+ + Sbjct: 260 LWYQIVDKGDRSDNWLESSGSCLYMYAIAKGINKGYLDRAYETTLLKAYQGLIQHKTETS 319 Query: 180 EQGRLILPDICIGTSAGDYENYVTRPKVSNDLHGVGALVMACVEMQSLYDEA 231 E G ++ DIC+GTSAG Y+ YV+R + +NDLHG GA ++A E++ L+ A Sbjct: 320 EDGAFLVKDICVGTSAGFYDYYVSRERSTNDLHGAGAFILAMTELEPLFRSA 371 >B4ANQ9.1 B4ANQ9_BACPU Glycosyl hydrolase, family 88 EC=3.2.1.- Length = 370 Score = 258 bits (658), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 121/228 (53%), Positives = 155/228 (67%), Gaps = 1/228 (0%) Query: 1 MWLDGLYMAGVFSLKYANTYGETSLRETVLHQERLMRRHMKDEATGLLYHAWDESKRMPW 60 MWLDGLYM G F+LKYA E L + V+ QE LMR+H KDE TGL YHAWDE K MPW Sbjct: 137 MWLDGLYMGGPFALKYAKLKDEEELVDMVIQQEHLMRKHTKDEKTGLYYHAWDERKVMPW 196 Query: 61 ANPRTGCSPEFWSRSLGWYGLAVSQFLDLLPAGDPGRAELAAELSGFVEALIRYQDRESG 120 A+ +TGCSPEFW+RS GWY LA++ ++ LP GR L+ +E++ RYQD+++G Sbjct: 197 ADQKTGCSPEFWARSFGWYVLALADMIEDLPEQHEGRKMWKETLTDMLESVARYQDQDTG 256 Query: 121 LWYQVVDKGDQPDNWLETSGSCLFVYTIAKAVKHGIAPPGALAAARKGYEGLIR-VLQQD 179 LW+Q++DKG DNWLE+SGSCLF+Y +AKA+ G P K Y+GLI+ Sbjct: 257 LWHQIIDKGTHSDNWLESSGSCLFMYAMAKAMNEGYVSPRYTDHVVKAYQGLIQHKTAVT 316 Query: 180 EQGRLILPDICIGTSAGDYENYVTRPKVSNDLHGVGALVMACVEMQSL 227 E G L + DICIGTSAG Y+ YV R + +NDLHG GA +MA E++ L Sbjct: 317 ENGDLTINDICIGTSAGFYDYYVGRERSTNDLHGAGAFIMALTELEKL 364 >A8FGF0.1 A8FGF0_BACP2 Unsaturated glucuronyl hydrolase Length = 370 Score = 257 bits (657), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 121/228 (53%), Positives = 155/228 (67%), Gaps = 1/228 (0%) Query: 1 MWLDGLYMAGVFSLKYANTYGETSLRETVLHQERLMRRHMKDEATGLLYHAWDESKRMPW 60 MWLDGLYM G F+LKYA GE L + V+HQE LMR+H KDE TGL YHAWDE K MPW Sbjct: 137 MWLDGLYMGGPFALKYAKLKGEEELVDMVIHQEYLMRKHTKDEKTGLYYHAWDERKVMPW 196 Query: 61 ANPRTGCSPEFWSRSLGWYGLAVSQFLDLLPAGDPGRAELAAELSGFVEALIRYQDRESG 120 A+ +TGCSPEFW+RS GWY LA++ ++ LP GR L +E++ RYQD+++G Sbjct: 197 ADQKTGCSPEFWARSFGWYVLALADMIEDLPEQHEGRKMWKETLIDMLESVARYQDQDTG 256 Query: 121 LWYQVVDKGDQPDNWLETSGSCLFVYTIAKAVKHGIAPPGALAAARKGYEGLIR-VLQQD 179 LW+Q++DKG DNWLE+SGSCLF+Y +AKA+ G K Y+GLI+ + Sbjct: 257 LWHQIIDKGTHSDNWLESSGSCLFMYAMAKAMNEGYVSLRYTDHVVKAYQGLIKHKTAET 316 Query: 180 EQGRLILPDICIGTSAGDYENYVTRPKVSNDLHGVGALVMACVEMQSL 227 E G + DICIGTSAG Y+ YV R + +NDLHG GA +MA E++ L Sbjct: 317 ETGDFAINDICIGTSAGFYDYYVGRERSTNDLHGAGAFIMALTELEKL 364 >Q65FY8.1 Q65FY8_BACLD YteR Length = 373 Score = 254 bits (648), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 119/227 (52%), Positives = 158/227 (69%), Gaps = 1/227 (0%) Query: 1 MWLDGLYMAGVFSLKYANTYGETSLRETVLHQERLMRRHMKDEATGLLYHAWDESKRMPW 60 MWLDGLYM G F+LKYA + E L + V+ +E+LMR+H KD TGL YHAWDE ++MPW Sbjct: 137 MWLDGLYMGGPFALKYARAFNEPELYDKVILEEKLMRKHTKDCRTGLYYHAWDEKRQMPW 196 Query: 61 ANPRTGCSPEFWSRSLGWYGLAVSQFLDLLPAGDPGRAELAAELSGFVEALIRYQDRESG 120 A+P +GCSPEFW RS+GWY LA+S ++LLP G R E L V+++ +QD +G Sbjct: 197 ADPVSGCSPEFWGRSIGWYALALSDMIELLPQGHSTRQEWTRTLQEMVKSIADFQDESTG 256 Query: 121 LWYQVVDKGDQPDNWLETSGSCLFVYTIAKAVKHGIAPPGALAAARKGYEGLI-RVLQQD 179 LW+QVVDKG PDNWLE+S SCL++Y +AK + G + A K ++GL+ ++ Sbjct: 257 LWHQVVDKGGLPDNWLESSCSCLYMYAMAKGARMGYIDSSYIDRATKAFKGLVEEKIEMS 316 Query: 180 EQGRLILPDICIGTSAGDYENYVTRPKVSNDLHGVGALVMACVEMQS 226 + L+L DIC+GTSAG Y+ YV R K +NDLHGVGALVMA VE++ Sbjct: 317 AKEGLLLKDICVGTSAGTYDYYVGREKSTNDLHGVGALVMAFVELEK 363 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,471,212,915 Number of extensions: 60679220 Number of successful extensions: 135561 Number of sequences better than 1.0e-05: 280 Number of HSP's gapped: 135223 Number of HSP's successfully gapped: 280 Length of query: 233 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 102 Effective length of database: 1,826,543,238 Effective search space: 186307410276 Effective search space used: 186307410276 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)