BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0003.p (518 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D8A7.1 B1D8A7_9BACL Extracellular solute-binding protein family 1 720 0.0 Q5WIX3.1 Q5WIX3_BACSK Sugar ABC transporter substrate-binding pr... 531 e-149 Q8EPL0.1 Q8EPL0_OCEIH Hypothetical conserved protein 529 e-148 B3KFU2.1 B3KFU2_9BACI Extracellular solute-binding protein family 1 524 e-146 B3KFU1.1 B3KFU1_9BACI Extracellular solute-binding protein family 1 474 e-132 >B1D8A7.1 B1D8A7_9BACL Extracellular solute-binding protein family 1 Length = 521 Score = 720 bits (1859), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/457 (74%), Positives = 394/457 (86%), Gaps = 1/457 (0%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 MLPLN +TPPDTIK E+EKLTNTKLTYQF+PADTYE+KLN++ ATG LPQVTYLKNQ T Sbjct: 66 MLPLNVAQTPPDTIKNEIEKLTNTKLTYQFFPADTYEDKLNTTLATGGLPQVTYLKNQAT 125 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 F+QMK +++DGQFWEIGP L+EFPNL+KLKP ILNNTKVDGKLY+LYIGRPLARQGIIYR Sbjct: 126 FVQMKGAIRDGQFWEIGPYLNEFPNLSKLKPLILNNTKVDGKLYSLYIGRPLARQGIIYR 185 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 KDWAD LGL+ P VDE+ AMAKAFTE+DPDG+ K +T G+ DRN+L+YGAFKT++SWF Sbjct: 186 KDWADNLGLKTPETVDEVIAMAKAFTEKDPDGDKKANTIGLADRNDLIYGAFKTLASWFA 245 Query: 242 TPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAGM 301 TPN+WG+KDGQL P+F FPQY MD K+ RDAG MN+DFAATSKTD +++F SGKAG+ Sbjct: 246 TPNNWGDKDGQLQPDFTFPQYTVAMDAMKQIRDAGAMNKDFAATSKTDQISLFTSGKAGL 305 Query: 302 YIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKDE 361 YI GSM D+D+LNKDLVKN PNAV+DT +++ GP+G+ A W IPGYNNVVLFPKSA+KDE Sbjct: 306 YI-GSMADVDSLNKDLVKNVPNAVVDTLALIKGPNGEQATWAIPGYNNVVLFPKSAIKDE 364 Query: 362 AELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVIG 421 AELKK+LAFFDK MTPEVAN+M+WGIEGTHYTV DGKAK D +LIEREVKG+KDS+IG Sbjct: 365 AELKKVLAFFDKQMTPEVANMMFWGIEGTHYTVEDGKAKVTDASDLIEREVKGYKDSLIG 424 Query: 422 EAETNGMYQSLNVLPGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQIISDATY 481 EAETNGMY+ LP RIHAEE EN K+ + DPTAALDS TYT KGVELQ II+DATY Sbjct: 425 EAETNGMYEGFYSLPARIHAEEFTKENEKIAILDPTAALDSETYTDKGVELQTIITDATY 484 Query: 482 KYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYKK 518 KYIYG+IDKAGFEK V+ WK+RGG KIIEE+NA KK Sbjct: 485 KYIYGEIDKAGFEKAVEDWKKRGGDKIIEEFNAANKK 521 >Q5WIX3.1 Q5WIX3_BACSK Sugar ABC transporter substrate-binding protein Length = 504 Score = 531 bits (1368), Expect = e-149, Method: Compositional matrix adjust. Identities = 253/457 (55%), Positives = 327/457 (71%), Gaps = 1/457 (0%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 M L+T E P ++ +E+ TNT+L Q+ P YEE+LN++FATG+LPQ Y+KNQTT Sbjct: 48 MANLHTAEVPEKEVQHLLEEATNTELDIQWVPDSNYEERLNTAFATGTLPQAVYMKNQTT 107 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 + Q K++++DGQFWEIGP L EF NL KLKPE++ NT VDGK+Y LY GRPLAR GIIYR Sbjct: 108 YDQFKDAIRDGQFWEIGPYLEEFENLKKLKPEVVENTMVDGKVYALYQGRPLARSGIIYR 167 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 KDWAD LGL+ P ++E MA+AFTE DPDGNG+ DT G+ DRN+L+YGAFKTV+SW G Sbjct: 168 KDWADNLGLEAPTTIEEFAEMARAFTEDDPDGNGRDDTIGLTDRNDLIYGAFKTVASWHG 227 Query: 242 TPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAGM 301 TPN WGEKDG+L PEF F +Y DT+DFFK+ + GYMN+DF TSK D + +G AG+ Sbjct: 228 TPNGWGEKDGELLPEFMFDEYIDTLDFFKELHENGYMNKDFPVTSKPDQQALLKNGTAGI 287 Query: 302 YIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKDE 361 Y+ GSM D+ TL + + P+ D H+ V GPDG++ W IPGY N+V+FPKSAV+ E Sbjct: 288 YV-GSMGDVLTLYSEASQINPDLEFDVHNYVEGPDGEYNIWAIPGYGNLVIFPKSAVQTE 346 Query: 362 AELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVIG 421 ELK IL+F+D++M PE ANL++WGIE HYTV DGKA +D + EREVK ++ IG Sbjct: 347 EELKSILSFYDQLMEPENANLIFWGIEDEHYTVEDGKAVAIEDTQRTEREVKPYQSIEIG 406 Query: 422 EAETNGMYQSLNVLPGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQIISDATY 481 EAETNG Y + P ++ A+EL ++N V DPT L S T + G LQQII+DATY Sbjct: 407 EAETNGRYLGQSHYPVKLKADELEIDNESYLVHDPTTTLHSPTAQTHGERLQQIINDATY 466 Query: 482 KYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYKK 518 +YI GQ D AGFE+ V+ W+ +GG +I EYN Y + Sbjct: 467 QYILGQTDLAGFEQAVETWRSQGGDDMISEYNESYNQ 503 >Q8EPL0.1 Q8EPL0_OCEIH Hypothetical conserved protein Length = 504 Score = 529 bits (1363), Expect = e-148, Method: Compositional matrix adjust. Identities = 247/457 (54%), Positives = 327/457 (71%), Gaps = 1/457 (0%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 M L+T E P D I +E+ TNT++ +Q+ P + YE++LN++FAT +LP+ ++KNQTT Sbjct: 47 MANLHTPEVPGDVILDILEEKTNTEIEFQWVPDNNYEDRLNTAFATNTLPEAVFMKNQTT 106 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 F+Q K++++D QFWE+GP L EF NL LK E+L NTKVDGK+Y+LY GRPL+RQGIIYR Sbjct: 107 FMQFKDAIRDDQFWEVGPYLEEFENLGNLKEEVLENTKVDGKIYSLYQGRPLSRQGIIYR 166 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 KDWAD LGL P DE MA+AFTE DPDGNGK DT G+ DR++LVYGAFKTVSSWFG Sbjct: 167 KDWADNLGLSAPTTTDEFMEMARAFTEDDPDGNGKDDTFGLTDRSDLVYGAFKTVSSWFG 226 Query: 242 TPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAGM 301 TPN+WGE+DG+L PEF FP+Y MDFFK+ + GY+N+DF TSK D +G +G+ Sbjct: 227 TPNNWGEQDGELVPEFMFPEYKQAMDFFKEMHENGYINKDFPVTSKVDQQEFIKNGSSGI 286 Query: 302 YIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKDE 361 Y+ GSM D+ TL+ D P+ V D H+ V GP+G++ W IPGY N+V+FPKS V+ E Sbjct: 287 YV-GSMGDVVTLHSDAAAINPDVVYDVHNQVEGPNGEYNTWAIPGYGNLVMFPKSTVESE 345 Query: 362 AELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVIG 421 ELK+IL FFD +M PE++N +YWG+EG HYTV +G A P +D+ L +REVK ++ IG Sbjct: 346 EELKRILGFFDALMDPELSNSIYWGVEGEHYTVKEGGAVPTEDQALFDREVKPYQAIEIG 405 Query: 422 EAETNGMYQSLNVLPGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQIISDATY 481 EAETNG Y+ + +EEL +N + DPT LDS T G L QI++DATY Sbjct: 406 EAETNGRYEGFFEYEPKAKSEELFKDNENYLIHDPTITLDSETNIQDGSRLLQIMTDATY 465 Query: 482 KYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYKK 518 +YI GQID+ GF+ ++ WK +GG+ IIEE+NA Y + Sbjct: 466 QYILGQIDEEGFDSAIENWKSQGGEAIIEEFNASYAE 502 >B3KFU2.1 B3KFU2_9BACI Extracellular solute-binding protein family 1 Length = 511 Score = 524 bits (1349), Expect = e-146, Method: Compositional matrix adjust. Identities = 241/457 (52%), Positives = 340/457 (74%), Gaps = 2/457 (0%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 M L+T E P D I+ +E+ T TKL Q+ P TY+EK+N+SFATG+LPQVTYLKN + Sbjct: 53 MANLHTPEVPSDMIEKLLEEKTGTKLDIQWVPDGTYDEKVNASFATGTLPQVTYLKNAAS 112 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 + M++++++GQFWEIGPLL ++PNL+KLKP++L NT VDGK+Y LY PL+RQGIIYR Sbjct: 113 LVNMRDAIRNGQFWEIGPLLDQYPNLSKLKPDVLKNTAVDGKIYALYREVPLSRQGIIYR 172 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 KDWADKLGL P N+DE + M K F E+DPDGNG+ DT + DRN+L+YGAFKT+SSW G Sbjct: 173 KDWADKLGLSAPTNIDEFYNMLKQFKEKDPDGNGQDDTIPLTDRNDLIYGAFKTISSWLG 232 Query: 242 TPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAGM 301 TPN+WGEKDG+LAPEF FP+Y +TM FFKK G +NQDF TSKTD N+F++GK+G+ Sbjct: 233 TPNNWGEKDGKLAPEFMFPEYMETMKFFKKLHQEGLINQDFPVTSKTDQQNLFITGKSGV 292 Query: 302 YIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKDE 361 YI G+M D+ +L+ +V+ P+A LD + + G F W +PGY +V+LFPK+A+K E Sbjct: 293 YI-GAMGDVSSLHPKVVEVNPDAELDVQNKIEGGPNGFGIWSVPGYGSVILFPKTAIKSE 351 Query: 362 AELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVIG 421 ELK +LAF D++M+PE+ NL+YWG+EG H+ + +GK P++D +L +REVK ++ +G Sbjct: 352 EELKGVLAFMDQLMSPELGNLIYWGVEGQHHQLEEGKVVPSEDTKLTDREVKPYQAMQVG 411 Query: 422 EAET-NGMYQSLNVLPGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQIISDAT 480 T G Y+ ++LP + AEE+++EN + + DP+A+LDS T+ KGV+LQ++I D T Sbjct: 412 GPLTIEGFYEPKHMLPVKAKAEEMIVENNEYLIEDPSASLDSKTFNEKGVQLQEMIKDGT 471 Query: 481 YKYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYK 517 Y+++ G +D+AGF+ V W + GGQ+II+E+N Y+ Sbjct: 472 YRFMLGDLDEAGFQAVVDNWLKSGGQQIIDEFNVSYE 508 >B3KFU1.1 B3KFU1_9BACI Extracellular solute-binding protein family 1 Length = 513 Score = 474 bits (1221), Expect = e-132, Method: Compositional matrix adjust. Identities = 234/457 (51%), Positives = 309/457 (67%), Gaps = 3/457 (0%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 M L+TTE P D I+ E+E+ T +L Q+ P +Y+EK+ ++ ATG+LPQV YLKN + Sbjct: 47 MANLHTTEIPSDRIEKEIEEKTGVQLDIQWVPDGSYDEKVFAALATGNLPQVLYLKNAAS 106 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 ++ ++ G FWEIGP L +PNL +LKPE+L NT +DGKLY+LY R LAR GIIYR Sbjct: 107 LSYFRDEIRSGLFWEIGPYLDGYPNLKRLKPEVLRNTAIDGKLYSLYQERALARSGIIYR 166 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 KDWAD+LGL P +D+L+ M K FT DPD NG DT G+ DRN+L+YGAFKT+SS++G Sbjct: 167 KDWADRLGLPAPETLDDLYEMLKGFTYNDPDNNGIDDTIGLTDRNDLIYGAFKTISSYYG 226 Query: 242 TPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAGM 301 TPN WG + L PEF FPQY +TM FFKK G +N+DF TSKTD +F++GKAG+ Sbjct: 227 TPNRWGWYNDSLQPEFVFPQYMETMKFFKKLHSEGLVNEDFPITSKTDQQELFITGKAGV 286 Query: 302 YIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKDE 361 YI G+M D+ +L L+ N P AVLD + V GP+G F W GY V+LFPKSA+ E Sbjct: 287 YI-GAMGDVLSLQSRLLANDPEAVLDVQNRVRGPEG-FGIWASQGYGTVILFPKSAIATE 344 Query: 362 AELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVIG 421 ELK +L+F+D +M+PE+ANLMYWGIEGTHYTV DGKA D EL E++VK ++ + G Sbjct: 345 EELKNVLSFYDALMSPELANLMYWGIEGTHYTVQDGKAVAVDAFELREKDVKPYQALMAG 404 Query: 422 EAET-NGMYQSLNVLPGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQIISDAT 480 T GM + +N + AE L+ +N + DPTA L+S Y GV L + + DAT Sbjct: 405 SFSTIPGMLEPVNTNKVKDKAERLIYDNESFLIYDPTAPLESPLYNEIGVRLNERMRDAT 464 Query: 481 YKYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYK 517 Y+++ G ID+ GFE E+ W E GG +IIEEY Y+ Sbjct: 465 YQFMLGMIDEKGFEAEIDRWLEEGGSQIIEEYETSYR 501 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,993,659,438 Number of extensions: 133703242 Number of successful extensions: 232097 Number of sequences better than 1.0e-05: 252 Number of HSP's gapped: 231860 Number of HSP's successfully gapped: 252 Length of query: 518 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 378 Effective length of database: 1,741,754,103 Effective search space: 658383050934 Effective search space used: 658383050934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 134 (56.2 bits)