BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0024.p (288 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8T4.1 B3K8T4_9BACI 60 kDa inner membrane insertion protein 402 e-110 B1DC08.1 B1DC08_9BACL 60 kDa inner membrane insertion protein 329 2e-88 A6CPN5.1 A6CPN5_9BACI OxaA-like protein 216 2e-54 B1YGA9.1 B1YGA9_EXIS2 60 kDa inner membrane insertion protein 209 2e-52 Q8EKU1.1 OXAA_OCEIH Membrane protein oxaA 204 1e-50 >B3K8T4.1 B3K8T4_9BACI 60 kDa inner membrane insertion protein Length = 296 Score = 402 bits (1033), Expect = e-110, Method: Compositional matrix adjust. Identities = 186/237 (78%), Positives = 213/237 (89%) Query: 1 MVLMIAVLSGCAPASTVTHTTEDLKNGGFWQSNVVYYFAIALDTFAEWFNGQYGFAVLVM 60 ++L +AV+SGCAP + T T EDLKNGGFWQSNVVYYF ALDTFA WFNG+YG +VLVM Sbjct: 17 VILAVAVISGCAPGADQTRTIEDLKNGGFWQSNVVYYFTWALDTFATWFNGEYGLSVLVM 76 Query: 61 VIIVRTLILPLTMKQVKSSRAMQAIQPELEKIKKKYKDTPEKVQQETMRLFQENKVNPMA 120 V+IVRTLILPLT+KQV+SS+AMQAIQPE++KI++KYKD PEKVQ ETM+LFQENKVNPMA Sbjct: 77 VLIVRTLILPLTLKQVRSSKAMQAIQPEIQKIREKYKDQPEKVQMETMKLFQENKVNPMA 136 Query: 121 GCLPLIVQMPIFIALYNSIYYNPSLREHSFLWLQLGKPDHLFILPLLAAATTFLQTRMMT 180 GCLPLIVQMPIFIALY+SIYYN LR+H FLWLQLG+PD LFILP+LAAATT+ QT+MM Sbjct: 137 GCLPLIVQMPIFIALYHSIYYNNLLRDHEFLWLQLGQPDKLFILPVLAAATTYFQTKMMM 196 Query: 181 KMNPVQQQGPMQFMMMVYPVLIFIMSYNFPSALPLYWFYSNLYTIIQNYFLYRNNDK 237 KMNP Q G MQFM+ VYP+LIF+MS+ FPSALPLYWFYSNLYTI+QNYFLYRNNDK Sbjct: 197 KMNPSPQMGMMQFMLFVYPILIFVMSFQFPSALPLYWFYSNLYTIVQNYFLYRNNDK 253 >B1DC08.1 B1DC08_9BACL 60 kDa inner membrane insertion protein Length = 258 Score = 329 bits (844), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 155/240 (64%), Positives = 198/240 (82%), Gaps = 3/240 (1%) Query: 1 MVLMIAVLSGCAPASTVTHTTEDLKNGGFWQSNVVYYFAIALDTFAEWFNGQYGFAVLVM 60 MVLM+ +LSGCAP + T +T DL++G FW+ NVVYYFA+ LD FA+WFNG YG A+L++ Sbjct: 16 MVLMVVLLSGCAPHNATT-STSDLQHGNFWEKNVVYYFALTLDKFADWFNGSYGLAILLI 74 Query: 61 VIIVRTLILPLTMKQVKSSRAMQAIQPELEKIKKKYKDTPEKVQQETMRLFQENKVNPMA 120 IIVRT ILPLT+KQ +SS+AMQAIQP++ +IKKK+KD P+K Q+ETM+LFQ NKVNPMA Sbjct: 75 TIIVRTAILPLTLKQYRSSKAMQAIQPQMAEIKKKHKDNPQKQQEETMKLFQANKVNPMA 134 Query: 121 GCLPLIVQMPIFIALYNSIYYNPSLREHSFLWLQLGKPDHLFILPLLAAATTFLQTRMMT 180 GCLPL+VQMP+F+ALYNSIY+NP +R H FL LQLG+P + LP++AAATTF+Q++MM Sbjct: 135 GCLPLLVQMPVFMALYNSIYWNPEVRTHEFLGLQLGEPGPWYFLPIIAAATTFVQSKMMQ 194 Query: 181 KMNPVQQQGPMQFMMMVYPVLIFIMSYNFPSALPLYWFYSNLYTIIQNYFLY-RNNDKTK 239 K G M M+M++PVLIF+M+ NFPSALPLYW +SNLYTIIQNYFLY R+++K K Sbjct: 195 KQQTQTMPG-MGAMLMIFPVLIFVMALNFPSALPLYWIFSNLYTIIQNYFLYVRSSNKGK 253 >A6CPN5.1 A6CPN5_9BACI OxaA-like protein Length = 238 Score = 216 bits (550), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 105/216 (48%), Positives = 151/216 (69%), Gaps = 9/216 (4%) Query: 21 TEDLKNGGFWQSNVVYYFAIALDTFAEWFNGQYGFAVLVMVIIVRTLILPLTMKQVKSSR 80 TED N GFW +VY ++ + +E+F+G YG ++++ +I+R ILPL +KQ K+S+ Sbjct: 8 TED--NTGFWNEYIVYPLSLLIIKVSEFFDGSYGLGIIIVTLIIRLAILPLMIKQTKNSK 65 Query: 81 AMQAIQPELEKIKKKY--KD--TPEKVQQETMRLFQENKVNPMAGCLPLIVQMPIFIALY 136 AMQA+QPE++K+++KY KD T +K+QQETM LFQ++ VNP+AGC PLIVQMPI I Y Sbjct: 66 AMQALQPEMQKLREKYSSKDAQTQQKLQQETMGLFQKHGVNPLAGCFPLIVQMPILIGFY 125 Query: 137 NSIYYNPSLREHSFLWLQLGKPDHLFILPLLAAATTFLQTRMMTKMNPVQQQGP-MQFMM 195 ++I ++ H+FLW LG+PD L++LPL+A TTF+Q +MM M Q P M M+ Sbjct: 126 HAIVRTEEIKNHTFLWFDLGQPDPLYLLPLIAGVTTFIQQKMM--MAGTANQNPQMAMML 183 Query: 196 MVYPVLIFIMSYNFPSALPLYWFYSNLYTIIQNYFL 231 + PV+I I + NFP+AL LYW N++ IIQ YF+ Sbjct: 184 YIMPVMIIIFAINFPAALSLYWVVGNIFMIIQTYFI 219 >B1YGA9.1 B1YGA9_EXIS2 60 kDa inner membrane insertion protein Length = 284 Score = 209 bits (533), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 101/230 (43%), Positives = 149/230 (64%), Gaps = 6/230 (2%) Query: 1 MVLMIAVLSGCAPASTVTHTTEDLKNGGFWQSNVVYYFAIALDTFAEWFNGQYGFAVLVM 60 M ++ VLSGC P + T GFW+ V+ A + + +FNG YG ++V Sbjct: 14 MSVLAVVLSGCTPGTYGTGEPITGDTEGFWKHYFVWPMAWLIREISHFFNGDYGIGIIVT 73 Query: 61 VIIVRTLILPLTMKQVKSSRAMQAIQPELEKIKKKY----KDTPEKVQQETMRLFQENKV 116 ++VR +ILPL +KQ KS MQ IQPE+ K+++KY ++T +K+QQE M+L+QE+ V Sbjct: 74 TLLVRLVILPLMIKQTKSMGGMQVIQPEMVKLREKYSSKDQETQQKLQQEMMKLYQEHNV 133 Query: 117 NPMAGCLPLIVQMPIFIALYNSIYYNPSLREHSFLWLQLGKPDHLFILPLLAAATTFLQT 176 NP+AGCLP+++QMPI IA YN+I Y P++ +H+FLW LGKPD FILP+L+A T+LQ Sbjct: 134 NPLAGCLPILIQMPILIAFYNAIIYTPAIFQHTFLWFDLGKPDPYFILPILSAIFTYLQQ 193 Query: 177 RMMTKMNPVQQQGPMQFMMMVYPVLIFIMSYNFPSALPLYWFYSNLYTII 226 ++ M M+ M+ V+P++IF+M PSAL LYW +++II Sbjct: 194 KL--SMAGQDDNPQMKIMLYVFPIMIFVMGVTLPSALSLYWVVGYIFSII 241 >Q8EKU1.1 OXAA_OCEIH Membrane protein oxaA Length = 252 Score = 204 bits (518), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 102/242 (42%), Positives = 152/242 (62%), Gaps = 15/242 (6%) Query: 1 MVLMIAVLSGCAPASTVTHTTEDLKNGGFWQSNVVYYFAIALDTFAEWFNGQYGFAVLVM 60 ++ + +LSGC + D G W + VY + + A FN YG A++++ Sbjct: 11 LIALTTLLSGCTEINEPITPNSD----GIWNTIFVYPVSWLITNVAGVFNEGYGLAIIIV 66 Query: 61 VIIVRTLILPLTMKQVKSSRAMQAIQPELEKIKKKY----KDTPEKVQQETMRLFQENKV 116 + VR L++PL +KQ+KSS+AMQ IQP+L++I+KKY +T +K+QQETM LFQ+N V Sbjct: 67 TLFVRLLLMPLNVKQIKSSKAMQEIQPKLQEIQKKYTSKDANTQQKLQQETMELFQKNGV 126 Query: 117 NPMAGCLPLIVQMPIFIALYNSIYYNPSLREHSFLWLQLGKPDHLFILPLLAAATTFLQT 176 NP+AGCLP++VQMPIF+A+Y++I P + HSFLW QLG PD +ILP+L TFLQ Sbjct: 127 NPLAGCLPILVQMPIFVAMYHAIMRTPEISTHSFLWFQLGSPD--YILPILTGLFTFLQQ 184 Query: 177 RMMTKMNPVQQQGP-----MQFMMMVYPVLIFIMSYNFPSALPLYWFYSNLYTIIQNYFL 231 ++M N P MQ M+ V P++I +M++ FP+AL LYW N++ + Q + Sbjct: 185 KLMMSTNTSMNSNPQMKLQMQIMLYVMPIMIGVMAFFFPAALALYWVTGNIFMVFQTLLI 244 Query: 232 YR 233 + Sbjct: 245 NK 246 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,457,610,488 Number of extensions: 55087975 Number of successful extensions: 209711 Number of sequences better than 1.0e-05: 1887 Number of HSP's gapped: 206502 Number of HSP's successfully gapped: 1889 Length of query: 288 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 154 Effective length of database: 1,798,280,193 Effective search space: 276935149722 Effective search space used: 276935149722 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)