BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0022.p (458 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8T2.1 B3K8T2_9BACI tRNA modification GTPase TrmE 738 0.0 B1DC10.1 B1DC10_9BACL tRNA modification GTPase TrmE 645 0.0 Q9RCA7.1 MNME_BACHD tRNA modification GTPase mnmE EC=3.6.-.- 561 e-158 C0ZA65.1 C0ZA65_BREBN Probable tRNA modification GTPase 558 e-157 B4BPK2.1 B4BPK2_9BACI tRNA modification GTPase TrmE 554 e-156 >B3K8T2.1 B3K8T2_9BACI tRNA modification GTPase TrmE Length = 458 Score = 738 bits (1904), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/458 (79%), Positives = 410/458 (89%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M SDTIAA+STA+GEGGIAIIRVSGP ++ EV +F+SK LT+ ESHTVHYGHI+ P Sbjct: 1 MFSDTIAAISTAVGEGGIAIIRVSGPDSVREVERIFKSKNKLTQEESHTVHYGHIVDPKT 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 GE +EEVLVTVM+ PRSFTTEDVVEISAHGGVISV+RVMDLLLQ +IRLAEPGEFTKRAF Sbjct: 61 GENLEEVLVTVMRAPRSFTTEDVVEISAHGGVISVKRVMDLLLQLNIRLAEPGEFTKRAF 120 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAEAV+DLIRSKSDRAFSVALKQV G+LS KI++LRHTL+E LAHIEVNIDY Sbjct: 121 LNGRIDLSQAEAVMDLIRSKSDRAFSVALKQVEGTLSRKINELRHTLVETLAHIEVNIDY 180 Query: 181 PEHDVESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNAL 240 PEHDVESLT+EFIK+KS VM I++LLKTA EGKILREGITTAIVGRPNVGKSSLLN L Sbjct: 181 PEHDVESLTSEFIKEKSKLVMGEIDKLLKTAQEGKILREGITTAIVGRPNVGKSSLLNTL 240 Query: 241 ARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSDA 300 A+DN+AIVTDIPGTTRDVIEE+VTINNIPLKLLDTAGIRETMDVVE+IGVERSK A ++A Sbjct: 241 AQDNRAIVTDIPGTTRDVIEEFVTINNIPLKLLDTAGIRETMDVVEQIGVERSKHAVNEA 300 Query: 301 DLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPMS 360 DLILLVLNA++ LH+DEL LMEQI+GRQ +VIMNKMDLPS L+ D L Y+ + IVPMS Sbjct: 301 DLILLVLNASEPLHQDELELMEQIRGRQCIVIMNKMDLPSNLDLDILHRYYPDELIVPMS 360 Query: 361 VLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIPI 420 V +EG+D+LEEAIS LFF G++E GDLTYVSNVRHIALLKK+ +SLQDAY+AA+ LIPI Sbjct: 361 VKAQEGIDRLEEAISNLFFSGQIEGGDLTYVSNVRHIALLKKSKQSLQDAYDAAEQLIPI 420 Query: 421 DMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 DMIQIDVR+AWEQL EI+G+ DSL+DQIFSQFCLGK Sbjct: 421 DMIQIDVRMAWEQLSEILGEAVGDSLIDQIFSQFCLGK 458 >B1DC10.1 B1DC10_9BACL tRNA modification GTPase TrmE Length = 459 Score = 645 bits (1665), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/459 (70%), Positives = 387/459 (84%), Gaps = 1/459 (0%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTIAA+STA+GEGGIAIIRVSGP AI A +FRS+T L + +HTV YGHII P + Sbjct: 1 MVHDTIAAISTAVGEGGIAIIRVSGPDAIEGTARIFRSRTDLRDAATHTVQYGHIIDPKN 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMD-LLLQRDIRLAEPGEFTKRA 119 GE++EEV+VTVM+GPRSFT EDVVEI+AHGGV++V++V+D +LLQ +RLAEPGEFTKRA Sbjct: 61 GEQVEEVIVTVMRGPRSFTAEDVVEINAHGGVVAVKKVLDQVLLQDGMRLAEPGEFTKRA 120 Query: 120 FLGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNID 179 FL GRIDL QAEAVIDLIRSKSDRAFSVA KQ G+LS +I LR T+IE+LAHIEVNID Sbjct: 121 FLNGRIDLMQAEAVIDLIRSKSDRAFSVARKQAEGTLSKRIKALRQTVIELLAHIEVNID 180 Query: 180 YPEHDVESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNA 239 YPEHDVE LT+ +I+++S +E I +LLKTANEGKILREGI TAIVGRPNVGKSSLLN Sbjct: 181 YPEHDVEDLTSAYIREQSGSALEEIGKLLKTANEGKILREGIMTAIVGRPNVGKSSLLNM 240 Query: 240 LARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSD 299 LA++NKAIVTDIPGTTRDVIEEYVT+N+IPL+LLDTAGIRET DVVE+IGVERS A + Sbjct: 241 LAQENKAIVTDIPGTTRDVIEEYVTLNHIPLRLLDTAGIRETNDVVERIGVERSHNALEE 300 Query: 300 ADLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPM 359 ADLIL VLN + +HED+ AL+E+++ RQ ++I+NK DL S+LE ++ + E IV M Sbjct: 301 ADLILFVLNNNEPIHEDDRALLEKVKDRQLILIINKSDLESKLEMAEIEALIDEERIVRM 360 Query: 360 SVLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIP 419 SV EE+GLD+LE+AIS LFF G+L+SGDLTYVSNVRHI+LLK+A +SL DA +A++ IP Sbjct: 361 SVREEDGLDRLEKAISKLFFEGQLDSGDLTYVSNVRHISLLKRAQQSLIDAIDASNAGIP 420 Query: 420 IDMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 ID+IQIDVR AWE LGEI+GD DSL+DQIFSQFCLGK Sbjct: 421 IDLIQIDVRSAWESLGEILGDEVGDSLIDQIFSQFCLGK 459 >Q9RCA7.1 MNME_BACHD tRNA modification GTPase mnmE EC=3.6.-.- Length = 458 Score = 561 bits (1446), Expect = e-158, Method: Compositional matrix adjust. Identities = 268/458 (58%), Positives = 361/458 (78%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTIAA+STALGEG I I+R+SG +A+ ++R K PL EV SHT+ YGHI+ P Sbjct: 1 MEFDTIAAISTALGEGAIGIVRLSGDEAVQLADRVYRGKKPLQEVPSHTIVYGHIVDPKT 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 E ++EV+V+V++ PR+FT EDV+EI+ HGG++SV RV+ L+L RLAEPGEFTKRAF Sbjct: 61 DEIIDEVMVSVLRAPRTFTREDVIEINCHGGLVSVNRVLQLVLNHGARLAEPGEFTKRAF 120 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAE VIDLIR+K+DRA +VALKQV G LS KI LR L+E +AH+EVNIDY Sbjct: 121 LNGRIDLSQAEGVIDLIRAKTDRAMNVALKQVEGRLSTKISSLRQALLETVAHVEVNIDY 180 Query: 181 PEHDVESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNAL 240 PE+D E++T + +KD++ V + I++LL TA +GKILREG++T I+GRPNVGKSSLLN+L Sbjct: 181 PEYDAETMTLDMLKDRAQFVRKEIDKLLMTAQQGKILREGLSTVIIGRPNVGKSSLLNSL 240 Query: 241 ARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSDA 300 +NKAIVTDIPGTTRDVIEEYV + +PL+L+DTAGIRET D+VE+IGVERS+ +A Sbjct: 241 VHENKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSREVLKEA 300 Query: 301 DLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPMS 360 +LILLVLN +EL +++ AL E I G +A+VI+NK D+ +++ ++ + ++ S Sbjct: 301 ELILLVLNFGEELSKEDEALFEAISGMEAIVIVNKTDVEQKIDLTRVRELAAGRPVITTS 360 Query: 361 VLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIPI 420 +++++G+D+LE AI+ LFF +ESGD+TYVSN RHIALL++A ++++DA EA +M +PI Sbjct: 361 IVQDQGIDELEAAIASLFFESGIESGDVTYVSNSRHIALLEQAKRAVEDALEAIEMDVPI 420 Query: 421 DMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 DM+QID+ AWE LGEIIGD+ +SL+DQ+FSQFCLGK Sbjct: 421 DMVQIDITRAWELLGEIIGDSVHESLIDQLFSQFCLGK 458 >C0ZA65.1 C0ZA65_BREBN Probable tRNA modification GTPase Length = 458 Score = 558 bits (1438), Expect = e-157, Method: Compositional matrix adjust. Identities = 264/458 (57%), Positives = 352/458 (76%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTIAAV+T +GEGGIA+IRVSG +AI V +++ K L+ V+SHT+HYGH+ P Sbjct: 1 MKFDTIAAVATPMGEGGIAVIRVSGTEAIEVVDKIYKGKQRLSTVDSHTIHYGHLYEPNT 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 GER+EEVLV+VMK PR+FT EDVVE++ HGG++SV +V++L+L RLAEPGEFTKRAF Sbjct: 61 GERVEEVLVSVMKAPRTFTREDVVEVNCHGGIVSVEKVLELILDNGARLAEPGEFTKRAF 120 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GR+DLSQAEAVIDLIR+K+DRA VAL QV G LS I +LR LIE +AHIEV +DY Sbjct: 121 LNGRVDLSQAEAVIDLIRAKTDRAMKVALNQVEGKLSRLIRQLRQNLIEAMAHIEVTLDY 180 Query: 181 PEHDVESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNAL 240 PEHDVE T F++ K EV I +LL+TA +GKILREG++TAI+GRPNVGKSSLLN+L Sbjct: 181 PEHDVEEFTQNFLRGKCLEVKGEIQRLLQTAQQGKILREGLSTAIIGRPNVGKSSLLNSL 240 Query: 241 ARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSDA 300 ++ KAIVTD+ GTTRDVIEEYV + +PL+L+DTAGIR+T D+VEKIGVE+S+ A Sbjct: 241 VQEEKAIVTDVAGTTRDVIEEYVNVRGVPLRLIDTAGIRDTEDIVEKIGVEKSRQLLQKA 300 Query: 301 DLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPMS 360 DL+LLV+N + L D+ A+ E +G +VI+NK DLP +++ +++ +F + ++ S Sbjct: 301 DLVLLVINYNEPLSADDYAIFEAAKGFHVIVIVNKFDLPQKVDLEEIKRHFPQQPLIMTS 360 Query: 361 VLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIPI 420 EE G+D LE+AI +FF G+++ DLTYVSN RHI LL++A +++ +A D L+P+ Sbjct: 361 AREETGIDLLEQAIGEIFFSGRVQQDDLTYVSNARHIQLLRQAERAMDEALGGIDELMPV 420 Query: 421 DMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 DMIQID++ +WE LGE+IG++ + L+DQIFSQFCLGK Sbjct: 421 DMIQIDIKKSWELLGEVIGESVGEDLIDQIFSQFCLGK 458 >B4BPK2.1 B4BPK2_9BACI tRNA modification GTPase TrmE Length = 462 Score = 554 bits (1427), Expect = e-156, Method: Compositional matrix adjust. Identities = 274/458 (59%), Positives = 355/458 (77%), Gaps = 3/458 (0%) Query: 4 DTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKT--PLTEVESHTVHYGHIISPGDG 61 DTIAA+ST +GEG IAI+R+SG +A+ LFRS T L +V SHT+HYGHI+ P G Sbjct: 5 DTIAAISTPMGEGAIAIVRLSGDEAVEIADRLFRSPTGKQLKDVPSHTIHYGHIVDPKSG 64 Query: 62 ERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAFL 121 +EEV+V+VM+ P++FT EDVVEI+ HGG +SV RV+ L+L RLAEPGEFTKRAFL Sbjct: 65 RIVEEVMVSVMRAPKTFTREDVVEINCHGGFVSVNRVLQLVLANGARLAEPGEFTKRAFL 124 Query: 122 GGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDYP 181 GRIDLSQAEAVIDLIR+K+DRA +VAL+Q+ G LS I +LR T++E LAH+EVNIDYP Sbjct: 125 NGRIDLSQAEAVIDLIRAKTDRAMNVALQQMEGRLSKLIRELRQTILETLAHVEVNIDYP 184 Query: 182 EHD-VESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNAL 240 E+D VE +T +K+K+ V I +LL TA +GKILREG+ T I+GRPNVGKSSLLNAL Sbjct: 185 EYDDVEEMTPRLLKEKAEYVRGQIEKLLSTATQGKILREGLATVIIGRPNVGKSSLLNAL 244 Query: 241 ARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSDA 300 A +N+AIVTDIPGTTRDVIEEYV + +PL+L+DTAGIRET D+VE+IGVERS+ A Sbjct: 245 AHENRAIVTDIPGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQMLKKA 304 Query: 301 DLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPMS 360 DLILLVLN + L E++ L +G +VI+NK DLP ++ +++ + IV S Sbjct: 305 DLILLVLNYHEPLTEEDERLFAMTEGMDVIVIVNKTDLPQNIDIERVKELAAGRPIVATS 364 Query: 361 VLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIPI 420 +L E G+D+LE+AI+ LFFGG+LE+GDLTYVSN RHIALL++A K+++DA D+ +P+ Sbjct: 365 LLCERGIDELEKAIADLFFGGELEAGDLTYVSNSRHIALLEQAKKAIEDALSGIDVGMPV 424 Query: 421 DMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 D++QID+R AWE LGEI+GDT +SL+DQ+F+QFCLGK Sbjct: 425 DLVQIDLRRAWELLGEIVGDTVHESLIDQLFAQFCLGK 462 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,443,235,190 Number of extensions: 99156094 Number of successful extensions: 429249 Number of sequences better than 1.0e-05: 4948 Number of HSP's gapped: 423681 Number of HSP's successfully gapped: 6348 Length of query: 458 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 319 Effective length of database: 1,751,175,118 Effective search space: 558624862642 Effective search space used: 558624862642 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)