BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0020.p (240 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8T0.1 B3K8T0_9BACI Methyltransferase GidB 333 1e-89 B1DC12.1 B1DC12_9BACL Methyltransferase GidB 303 8e-81 P25813.1 RSMG_BACSU Ribosomal RNA small subunit methyltransferas... 266 1e-69 Q65CN3.1 RSMG_BACLD Ribosomal RNA small subunit methyltransferas... 264 4e-69 C0ZA63.1 RSMG_BREBN Ribosomal RNA small subunit methyltransferas... 262 2e-68 >B3K8T0.1 B3K8T0_9BACI Methyltransferase GidB Length = 240 Score = 333 bits (853), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 154/240 (64%), Positives = 184/240 (76%) Query: 1 MDNTAVQFTALLQAQGITLSPKQLEQFELYFSELVSWNEKMNLTGITEREQVYMKHFYDS 60 MD+ F L GIT+ KQL QFE+Y+ LV WNEKMNLTGITEREQVY KHFYDS Sbjct: 1 MDDIQKAFVDRLGEHGITIDEKQLHQFEMYYEVLVEWNEKMNLTGITEREQVYTKHFYDS 60 Query: 61 LSLAFFMNMGEVNTLADIGSGAGFPGIPLKICFPHLKLTIVDSLSKRISFLQHVCDTLQL 120 ++LAF+++M ++ LADIGSGAGFPGIPLKICFPHLKLTIVDSL+KRI+FL+HV DTL L Sbjct: 61 ITLAFYVDMNKIGNLADIGSGAGFPGIPLKICFPHLKLTIVDSLNKRITFLKHVADTLGL 120 Query: 121 KNVQLIHGRAEDVARQFIHRDAYDXXXXXXXXXXXXXNEFCLPFTRKDGLFAAMKGNDPS 180 ++LIHGRAE+VAR+ +RD +D NEFCLPFT+ G FAAMKG+DP+ Sbjct: 121 SGIELIHGRAEEVARKHGYRDGFDLVTARAVARMAVLNEFCLPFTKVGGQFAAMKGSDPT 180 Query: 181 EELTEAKRSFKELRAELQKVESFTLPVEESARHIVFIRKTGATPAKYPRKAGTPAKAPLI 240 +E+ EA RS KELR L KV SF+LPVEES RHIV I K GATP+KYPRKAGT +K P++ Sbjct: 181 DEVKEAARSLKELRGALNKVHSFSLPVEESGRHIVVIDKKGATPSKYPRKAGTASKTPIV 240 >B1DC12.1 B1DC12_9BACL Methyltransferase GidB Length = 240 Score = 303 bits (776), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 145/240 (60%), Positives = 175/240 (72%) Query: 1 MDNTAVQFTALLQAQGITLSPKQLEQFELYFSELVSWNEKMNLTGITEREQVYMKHFYDS 60 M T V F LL GI LSPKQLEQF+ Y LV WNEKMNLTGITER+ V+ KHFYDS Sbjct: 1 MTETHVWFEKLLGENGILLSPKQLEQFDSYHRLLVEWNEKMNLTGITERDAVFEKHFYDS 60 Query: 61 LSLAFFMNMGEVNTLADIGSGAGFPGIPLKICFPHLKLTIVDSLSKRISFLQHVCDTLQL 120 +SL+FF+NM EV ++ADIGSGAGFP IPLKIC+PHLK+ IVDSL+KRI FL H+ L L Sbjct: 61 ISLSFFVNMKEVGSIADIGSGAGFPSIPLKICYPHLKVVIVDSLNKRIQFLNHLVQELGL 120 Query: 121 KNVQLIHGRAEDVARQFIHRDAYDXXXXXXXXXXXXXNEFCLPFTRKDGLFAAMKGNDPS 180 +NV +HGRAED+AR +RD++D NEFCLPFTRK+G FAAMKG+ Sbjct: 121 ENVNCVHGRAEDIARMPAYRDSFDLVTARAVARLNVLNEFCLPFTRKNGTFAAMKGSQSV 180 Query: 181 EELTEAKRSFKELRAELQKVESFTLPVEESARHIVFIRKTGATPAKYPRKAGTPAKAPLI 240 EE+ EA S +EL+A+++ +F LP E+S R+IV I KT ATP KYPRKAGTP K PL+ Sbjct: 181 EEVKEAAVSLRELKAKVRAEHAFKLPFEQSERYIVLINKTDATPKKYPRKAGTPLKLPLV 240 >P25813.1 RSMG_BACSU Ribosomal RNA small subunit methyltransferase G EC=2.1.1.- Length = 239 Score = 266 bits (679), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 119/233 (51%), Positives = 165/233 (70%) Query: 7 QFTALLQAQGITLSPKQLEQFELYFSELVSWNEKMNLTGITEREQVYMKHFYDSLSLAFF 66 +FT+ L +GI+LSP+QLEQFELY+ LV WNEK+NLT ITE+++VY+KHFYDS++ AF+ Sbjct: 5 EFTSGLAEKGISLSPRQLEQFELYYDMLVEWNEKINLTSITEKKEVYLKHFYDSITAAFY 64 Query: 67 MNMGEVNTLADIGSGAGFPGIPLKICFPHLKLTIVDSLSKRISFLQHVCDTLQLKNVQLI 126 ++ +VNT+ D+G+GAGFP +P+KICFPHL +TIVDSL+KRI+FL+ + + LQL+N Sbjct: 65 VDFNQVNTICDVGAGAGFPSLPIKICFPHLHVTIVDSLNKRITFLEKLSEALQLENTTFC 124 Query: 127 HGRAEDVARQFIHRDAYDXXXXXXXXXXXXXNEFCLPFTRKDGLFAAMKGNDPSEELTEA 186 H RAE ++ R++YD +E CLP +K+GLF A+K EEL Sbjct: 125 HDRAETFGQRKDVRESYDIVTARAVARLSVLSELCLPLVKKNGLFVALKAASAEEELNAG 184 Query: 187 KRSFKELRAELQKVESFTLPVEESARHIVFIRKTGATPAKYPRKAGTPAKAPL 239 K++ L EL+ + SF LP+EES R+I+ IRK TP KYPRK GTP K+P+ Sbjct: 185 KKAITTLGGELENIHSFKLPIEESDRNIMVIRKIKNTPKKYPRKPGTPNKSPI 237 >Q65CN3.1 RSMG_BACLD Ribosomal RNA small subunit methyltransferase G EC=2.1.1.- Length = 239 Score = 264 bits (675), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 119/233 (51%), Positives = 162/233 (69%) Query: 7 QFTALLQAQGITLSPKQLEQFELYFSELVSWNEKMNLTGITEREQVYMKHFYDSLSLAFF 66 QFTA L+ +GI+LS QLEQFE Y+ LV WNEKMNLT ITE+++VY+KHFYDS++ +F+ Sbjct: 5 QFTAGLEKKGISLSSFQLEQFETYYEWLVEWNEKMNLTSITEKKEVYLKHFYDSIAASFY 64 Query: 67 MNMGEVNTLADIGSGAGFPGIPLKICFPHLKLTIVDSLSKRISFLQHVCDTLQLKNVQLI 126 ++ ++NTL D+G+GAGFP +P+KICFPHL +TIVDSL+KRI FL + D L+L+N Sbjct: 65 VDFKKMNTLCDVGAGAGFPSLPIKICFPHLHVTIVDSLNKRIHFLNQLSDALKLENTAFY 124 Query: 127 HGRAEDVARQFIHRDAYDXXXXXXXXXXXXXNEFCLPFTRKDGLFAAMKGNDPSEELTEA 186 H RAE R HR++YD +E CLP +KDGLF A+K EE+ Sbjct: 125 HDRAETFGRSKDHRESYDVVTARAVARLSVLSELCLPLVKKDGLFVALKAASADEEIETG 184 Query: 187 KRSFKELRAELQKVESFTLPVEESARHIVFIRKTGATPAKYPRKAGTPAKAPL 239 K++ K L +++ V SF LP+EES R+I+ I+K TP K+PRK GTP K+P+ Sbjct: 185 KKAIKTLGGKIETVHSFQLPIEESERNIIVIKKQSQTPKKFPRKPGTPNKSPI 237 >C0ZA63.1 RSMG_BREBN Ribosomal RNA small subunit methyltransferase G EC=2.1.1.- Length = 238 Score = 262 bits (669), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 126/237 (53%), Positives = 163/237 (68%) Query: 4 TAVQFTALLQAQGITLSPKQLEQFELYFSELVSWNEKMNLTGITEREQVYMKHFYDSLSL 63 T QF +L AQGI+L+ +Q EQF+ +F LV WNEKMNLTGITE QVY KHFYDS++ Sbjct: 2 TKEQFAEVLAAQGISLTDRQKEQFDHFFRLLVEWNEKMNLTGITEEGQVYNKHFYDSITP 61 Query: 64 AFFMNMGEVNTLADIGSGAGFPGIPLKICFPHLKLTIVDSLSKRISFLQHVCDTLQLKNV 123 AF+ +V ++ DIG GAGFP IPLKICFPHLK+TI+DSL+KR+SFLQHV L L+NV Sbjct: 62 AFYFPFDQVQSVVDIGGGAGFPSIPLKICFPHLKMTIIDSLNKRMSFLQHVAKELGLENV 121 Query: 124 QLIHGRAEDVARQFIHRDAYDXXXXXXXXXXXXXNEFCLPFTRKDGLFAAMKGNDPSEEL 183 +HGRAED ++ ++R+ +D +EFCLPF + G F A+KG + + EL Sbjct: 122 NPVHGRAEDRGQEAMYREKFDLVVARAVARLNLLSEFCLPFAKVGGHFVALKGAEITPEL 181 Query: 184 TEAKRSFKELRAELQKVESFTLPVEESARHIVFIRKTGATPAKYPRKAGTPAKAPLI 240 EAK++ K L + +KVE+F L E R+IV + K ATP YPRKAG PAK PL+ Sbjct: 182 AEAKKAIKTLGGKTRKVETFQLLEEAGERNIVIMEKIEATPKSYPRKAGVPAKKPLV 238 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,261,679,151 Number of extensions: 45495979 Number of successful extensions: 121707 Number of sequences better than 1.0e-05: 1325 Number of HSP's gapped: 120309 Number of HSP's successfully gapped: 1325 Length of query: 240 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 108 Effective length of database: 1,817,122,223 Effective search space: 196249200084 Effective search space used: 196249200084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)