BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0016.p (387 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8S5.1 B3K8S5_9BACI Cysteine desulfurase family protein 496 e-138 C0ZA58.1 C0ZA58_BREBN Aminotransferase class V family protein 385 e-105 B0K8H2.1 B0K8H2_THEP3 Cysteine desulfurase family protein 377 e-102 Q8R6L3.1 Q8R6L3_THETN Selenocysteine lyase 373 e-101 B7R9K0.1 B7R9K0_9THEO Cysteine desulfurase family protein 373 e-101 >B3K8S5.1 B3K8S5_9BACI Cysteine desulfurase family protein Length = 382 Score = 496 bits (1276), Expect = e-138, Method: Compositional matrix adjust. Identities = 232/381 (60%), Positives = 301/381 (79%) Query: 1 MEELVYLDHAATSWPKPPEVAAAMMDALQNSGANAGRGNHSLAIGAGRVMVRARNKLAEL 60 M+ +VYLDHAATSWPKPPEV AM A++ + AN GRG+H +A+ A RV+ R LA+L Sbjct: 1 MDPIVYLDHAATSWPKPPEVFEAMKRAMEEAAANPGRGSHRMAVKASRVLYGTRKTLADL 60 Query: 61 FSVSNAQDIAFTQNTTMGLNMAIQGTLQPGDHVITTMTEHNSVRRPLEFLRQTLGISIDY 120 F V N DIA T NTT LN+AI+G L+ GDHVI TM EHNSVRRPLE+L++T GI +DY Sbjct: 61 FGVKNPNDIALTSNTTEALNLAIKGCLREGDHVIATMIEHNSVRRPLEYLKRTRGIQVDY 120 Query: 121 VQVDPEGQVDLLELQRTFRANTKMMICNHSSNLLGSILPIGEIGDIAQSKGALFLVDAAQ 180 V VD EGQ+DL ++ FR+NT++++C+HSSNLLGSI+P+ EIG++A+ KGA+ LVDAAQ Sbjct: 121 VPVDEEGQLDLQRIEGAFRSNTRLVVCSHSSNLLGSIIPLVEIGELAKKKGAILLVDAAQ 180 Query: 181 SAGSLTIDVKKMNIDLLAFPGHKGLLGPQGTGGLYIAPELDLIPLMQGGTGSQSENSGQP 240 SAG L I V+KM++ +LAFPGHKGLLGPQGTGGLYI+P++DL PL+ GGTGSQSE QP Sbjct: 181 SAGMLDIHVEKMHVGMLAFPGHKGLLGPQGTGGLYISPDIDLEPLLHGGTGSQSEAIEQP 240 Query: 241 TVRPDRYEAGTQNGVGIAGLLAGVTKIMSLGPENIHRQEWELTQKLMEGLSVIPGIRLLG 300 TVRPDRYEAGT N +G AGL AGV K++ P++I+R EWELTQ +M+GL + G+R+LG Sbjct: 241 TVRPDRYEAGTPNTIGYAGLQAGVQKVLEWTPQHIYRHEWELTQYMMDGLQEVRGLRILG 300 Query: 301 PKMGSERSGIVSFVVEGQDSADIAHRLDRRYNIAVRAGMHCTPLAHKAAYTLESGAVRAS 360 P +G R+GIV+FV E D+++IA RLDR Y IAVRAG HCTPLAH A+ T +GAVRAS Sbjct: 301 PALGKARTGIVAFVSEKFDASEIAFRLDREYGIAVRAGYHCTPLAHMASGTEHTGAVRAS 360 Query: 361 VGVTSTEEEVDRMIAAMLEMY 381 VG++++ +E++ M A+ E++ Sbjct: 361 VGISTSRDEIEFMRKAIKEIH 381 >C0ZA58.1 C0ZA58_BREBN Aminotransferase class V family protein Length = 384 Score = 385 bits (989), Expect = e-105, Method: Compositional matrix adjust. Identities = 181/377 (48%), Positives = 263/377 (69%) Query: 4 LVYLDHAATSWPKPPEVAAAMMDALQNSGANAGRGNHSLAIGAGRVMVRARNKLAELFSV 63 ++YLD+AA++WPKPP V M + +++ AN GRG H+LA+ A + + R R +++ LF V Sbjct: 3 IIYLDNAASTWPKPPAVKEMMAEVIEDFAANPGRGGHALAMKASKAVFRTRVQVSRLFGV 62 Query: 64 SNAQDIAFTQNTTMGLNMAIQGTLQPGDHVITTMTEHNSVRRPLEFLRQTLGISIDYVQV 123 N ++ F N T LN AI+G LQ GDHVI++ EHNSVRRP+EF+R+ + +V+ Sbjct: 63 QNPNNLFFYLNATQALNQAIKGFLQAGDHVISSSVEHNSVRRPIEFMRKNNQVEATFVEP 122 Query: 124 DPEGQVDLLELQRTFRANTKMMICNHSSNLLGSILPIGEIGDIAQSKGALFLVDAAQSAG 183 + Q + + + +T++++ +H+SNL G ILP+ E+G +A+ G FLVDA+QSAG Sbjct: 123 REDHQFYVEDFAQAITPSTRLIVVSHASNLTGVILPVAELGKLAKEHGITFLVDASQSAG 182 Query: 184 SLTIDVKKMNIDLLAFPGHKGLLGPQGTGGLYIAPELDLIPLMQGGTGSQSENSGQPTVR 243 L IDV+ MNI +LAFPGHKGL GPQGTGGLY++ ++DL PL+ GGTGSQSE QPT R Sbjct: 183 VLPIDVEAMNIHMLAFPGHKGLYGPQGTGGLYVSSDIDLEPLIHGGTGSQSEAIDQPTTR 242 Query: 244 PDRYEAGTQNGVGIAGLLAGVTKIMSLGPENIHRQEWELTQKLMEGLSVIPGIRLLGPKM 303 PDRYE+GT N VG+AGL AGV +M G +NI + EWEL ++ + GL I G+ + GP + Sbjct: 243 PDRYESGTLNTVGLAGLQAGVDFVMEKGVDNIRQHEWELVKQTILGLQQIEGVHVYGPGI 302 Query: 304 GSERSGIVSFVVEGQDSADIAHRLDRRYNIAVRAGMHCTPLAHKAAYTLESGAVRASVGV 363 ER G+V+F + D+A+++ LD++Y IA R+G HCTPL H+ A T + GAVRAS G+ Sbjct: 303 EIERVGVVAFNIGEVDAAEVSFILDQQYGIATRSGFHCTPLGHQTAGTEQRGAVRASFGI 362 Query: 364 TSTEEEVDRMIAAMLEM 380 ++E++V+ + A+ E+ Sbjct: 363 FNSEKDVEALHNAVREI 379 >B0K8H2.1 B0K8H2_THEP3 Cysteine desulfurase family protein Length = 380 Score = 377 bits (967), Expect = e-102, Method: Compositional matrix adjust. Identities = 180/374 (48%), Positives = 261/374 (69%), Gaps = 2/374 (0%) Query: 4 LVYLDHAATSWPKPPEVAAAMMDALQNSGANAGRGNHSLAIGAGRVMVRARNKLAELFSV 63 ++Y D+AATSWPKP EV + L+N G N GRG H +A+ +GRV+ AR ++ +F++ Sbjct: 1 MIYFDNAATSWPKPEEVYREVEKVLRNCG-NPGRGGHKMALESGRVIFEARQEICSIFNI 59 Query: 64 SNAQDIAFTQNTTMGLNMAIQGTLQPGDHVITTMTEHNSVRRPLEFLRQTLGISIDYVQV 123 + + FT NTT LN+A++G L+ GDHVIT+ EHNS+ RPL L++ GI + V+ Sbjct: 60 KDPMRVVFTSNTTEALNIALKGILKEGDHVITSSMEHNSMIRPLMALKEK-GIEVTIVKA 118 Query: 124 DPEGQVDLLELQRTFRANTKMMICNHSSNLLGSILPIGEIGDIAQSKGALFLVDAAQSAG 183 + EG++D +++ TKM+ H+SN+ G+I+PI EIG+IA+ +FLVDAAQ+ G Sbjct: 119 NEEGKIDPEDIKEAITKKTKMIAITHASNVTGTIMPIEEIGNIAREMNLIFLVDAAQTVG 178 Query: 184 SLTIDVKKMNIDLLAFPGHKGLLGPQGTGGLYIAPELDLIPLMQGGTGSQSENSGQPTVR 243 L IDV+K NIDLLAFPGHKGL GPQGTGGLY+ ++++PL +GGTGS+SE+ QP + Sbjct: 179 VLPIDVEKQNIDLLAFPGHKGLYGPQGTGGLYVRKSIEILPLEEGGTGSKSESMYQPDLM 238 Query: 244 PDRYEAGTQNGVGIAGLLAGVTKIMSLGPENIHRQEWELTQKLMEGLSVIPGIRLLGPKM 303 PD+ E+GT N GIAGL GV + S+G +NI++QE LT+ L+EGL I G+++ GP+ Sbjct: 239 PDKLESGTPNTPGIAGLKEGVKFVKSVGIKNIYQQEKRLTKILIEGLKEIKGVKIYGPQK 298 Query: 304 GSERSGIVSFVVEGQDSADIAHRLDRRYNIAVRAGMHCTPLAHKAAYTLESGAVRASVGV 363 ER G+VS ++ D +I++ LDR ++IA RAG+HC PLAH TL++G +R +G Sbjct: 299 VEERVGVVSITLKDSDVGEISYILDRDFDIATRAGLHCAPLAHSTIGTLKTGTLRFGIGY 358 Query: 364 TSTEEEVDRMIAAM 377 +TEEEV++ I A+ Sbjct: 359 FNTEEEVEKAIKAI 372 >Q8R6L3.1 Q8R6L3_THETN Selenocysteine lyase Length = 380 Score = 373 bits (957), Expect = e-101, Method: Compositional matrix adjust. Identities = 182/374 (48%), Positives = 259/374 (69%), Gaps = 2/374 (0%) Query: 4 LVYLDHAATSWPKPPEVAAAMMDALQNSGANAGRGNHSLAIGAGRVMVRARNKLAELFSV 63 ++Y D+AAT+WPKP EV + L+N G N GRG H +A+ A RV+ AR ++A LF++ Sbjct: 1 MIYFDNAATTWPKPEEVYLEVDRMLRNCG-NPGRGGHRMALEASRVIFEARQEIASLFNI 59 Query: 64 SNAQDIAFTQNTTMGLNMAIQGTLQPGDHVITTMTEHNSVRRPLEFLRQTLGISIDYVQV 123 + + FT NTT LN+A++G L+ GDHVIT+ EHNSV RPL L++ G+ + V+ Sbjct: 60 KDPMRVVFTLNTTEALNIALKGVLKEGDHVITSSMEHNSVARPLSRLKEQ-GVEVTIVKG 118 Query: 124 DPEGQVDLLELQRTFRANTKMMICNHSSNLLGSILPIGEIGDIAQSKGALFLVDAAQSAG 183 EG++D +++R + NTKM+ H+SN+ G+I+PI EIG+IA+ +FLVDAAQ+AG Sbjct: 119 SEEGKIDPEDIKRAVKKNTKMIAITHASNVTGTIMPIEEIGNIAREMNLIFLVDAAQTAG 178 Query: 184 SLTIDVKKMNIDLLAFPGHKGLLGPQGTGGLYIAPELDLIPLMQGGTGSQSENSGQPTVR 243 L IDV+K NI LLAFPGHKGL GPQGTGGLY+ ++L+PL +GGTGS+SE + QP + Sbjct: 179 VLPIDVEKYNISLLAFPGHKGLYGPQGTGGLYVKEGIELVPLKEGGTGSRSELTLQPDLM 238 Query: 244 PDRYEAGTQNGVGIAGLLAGVTKIMSLGPENIHRQEWELTQKLMEGLSVIPGIRLLGPKM 303 PD+ E+GT N GIAGL GV + +G +I +E +LT+ L+EGL I +R+ GP+ Sbjct: 239 PDKLESGTLNTPGIAGLKEGVKFVKKVGVNSIREREVKLTELLIEGLREIKKVRVYGPQK 298 Query: 304 GSERSGIVSFVVEGQDSADIAHRLDRRYNIAVRAGMHCTPLAHKAAYTLESGAVRASVGV 363 +R G+VS VEG D +++H LDR ++IA RAG+HC PLAH TL+SG +R +G Sbjct: 299 IEDRVGVVSITVEGMDVGEVSHILDREFDIATRAGLHCAPLAHSTIGTLKSGTLRFGIGY 358 Query: 364 TSTEEEVDRMIAAM 377 +TEEE+ + + A+ Sbjct: 359 FNTEEEIIKAVEAI 372 >B7R9K0.1 B7R9K0_9THEO Cysteine desulfurase family protein Length = 380 Score = 373 bits (957), Expect = e-101, Method: Compositional matrix adjust. Identities = 182/374 (48%), Positives = 259/374 (69%), Gaps = 2/374 (0%) Query: 4 LVYLDHAATSWPKPPEVAAAMMDALQNSGANAGRGNHSLAIGAGRVMVRARNKLAELFSV 63 ++Y D+AAT+WPKP EV + L+N G N GRG H +A+ A RV+ AR ++A LF++ Sbjct: 1 MIYFDNAATTWPKPEEVYLEVDRMLRNCG-NPGRGGHRMALEASRVIFEARQEIASLFNI 59 Query: 64 SNAQDIAFTQNTTMGLNMAIQGTLQPGDHVITTMTEHNSVRRPLEFLRQTLGISIDYVQV 123 + + FT NTT LN+A++G L+ GDHVIT+ EHNSV RPL L++ G+ + V+ Sbjct: 60 KDPMRVVFTLNTTEALNIALKGVLKEGDHVITSSMEHNSVARPLSRLKEQ-GVEVTIVKG 118 Query: 124 DPEGQVDLLELQRTFRANTKMMICNHSSNLLGSILPIGEIGDIAQSKGALFLVDAAQSAG 183 EG++D +++R + NTKM+ H+SN+ G+I+PI EIG+IA+ +FLVDAAQ+AG Sbjct: 119 SEEGKIDPEDIKRAVKKNTKMIAITHASNVTGTIMPIEEIGNIAREMNLIFLVDAAQTAG 178 Query: 184 SLTIDVKKMNIDLLAFPGHKGLLGPQGTGGLYIAPELDLIPLMQGGTGSQSENSGQPTVR 243 L IDV+K NI LLAFPGHKGL GPQGTGGLY+ ++L+PL +GGTGS+SE + QP + Sbjct: 179 VLPIDVEKYNISLLAFPGHKGLYGPQGTGGLYVKEGIELVPLKEGGTGSRSELTLQPDLM 238 Query: 244 PDRYEAGTQNGVGIAGLLAGVTKIMSLGPENIHRQEWELTQKLMEGLSVIPGIRLLGPKM 303 PD+ E+GT N GIAGL GV + +G +I +E +LT+ L+EGL I +R+ GP+ Sbjct: 239 PDKLESGTLNTPGIAGLKEGVKFVKKVGVNSIREREVKLTELLIEGLREIKKVRVYGPQK 298 Query: 304 GSERSGIVSFVVEGQDSADIAHRLDRRYNIAVRAGMHCTPLAHKAAYTLESGAVRASVGV 363 +R G+VS VEG D +++H LDR ++IA RAG+HC PLAH TL+SG +R +G Sbjct: 299 IEDRVGVVSITVEGMDVGEVSHILDREFDIATRAGLHCAPLAHSTIGTLKSGTLRFGIGY 358 Query: 364 TSTEEEVDRMIAAM 377 +TEEE+ + + A+ Sbjct: 359 FNTEEEIIKAVEAI 372 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,231,987,627 Number of extensions: 95697211 Number of successful extensions: 281355 Number of sequences better than 1.0e-05: 4898 Number of HSP's gapped: 269250 Number of HSP's successfully gapped: 4905 Length of query: 387 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 249 Effective length of database: 1,760,596,133 Effective search space: 438388437117 Effective search space used: 438388437117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)