BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0012.p (338 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8S1.1 B3K8S1_9BACI MscS Mechanosensitive ion channel 280 1e-73 B1DC21.1 B1DC21_9BACL MscS Mechanosensitive ion channel 196 3e-48 Q8EKV1.1 Q8EKV1_OCEIH Hypothetical conserved protein 175 7e-42 C0ZA53.1 C0ZA53_BREBN Conserved hypothetical membrane protein 174 8e-42 Q2B0Z4.1 Q2B0Z4_9BACI Putative uncharacterized protein 172 5e-41 >B3K8S1.1 B3K8S1_9BACI MscS Mechanosensitive ion channel Length = 300 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 137/264 (51%), Positives = 186/264 (70%) Query: 12 KEAVRFKDRVWNWVTDAEMWADVLFAGXXXXXXXXXXXXXXKVVYKVIDRSMEKESRGRL 71 +EA KD++W W+T+ EMW ++L +G +VVYK+ID+ + ++ + R+ Sbjct: 23 EEAEGLKDKIWGWLTNEEMWMNILSSGIRVIIIFILTRLVIRVVYKMIDQFLLRQEKSRI 82 Query: 72 LGDSRRFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXXXXXXXXXXXXXQSLV 131 +SRRF TVG LLKNV + +CNFVMI+++LSEF+F QSLV Sbjct: 83 QVNSRRFVTVGELLKNVTSVVCNFVMILIILSEFNFKLGPLLAGAGVLGLAIGFGAQSLV 142 Query: 132 KDVITGFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTGEVHIIPNGTIINVTN 191 KDVITGFFIIFEDQFAVGDVI++G Y+GTVEMIGLRT+R+ GE +IIPNG I +VTN Sbjct: 143 KDVITGFFIIFEDQFAVGDVIKSGEYRGTVEMIGLRTSRVRGLNGETYIIPNGMITSVTN 202 Query: 192 YSLANALAVVDVPVKIERGLEATLALIGEALKGIEERNSNVIAYPNILGIQSMSTSEYVI 251 YSL+N+LA+VD+PVK ++ ++ T+ LI AL GI +R V P++LGIQS++TSEYV+ Sbjct: 203 YSLSNSLAIVDLPVKNDQQVKDTITLIQSALTGIMDRRPEVQRTPDVLGIQSLTTSEYVV 262 Query: 252 RVAANCLPNARDAAERQIQSDIKR 275 R+ A C PNAR+A ERQI +DIK+ Sbjct: 263 RIVAECNPNAREAVERQIFTDIKQ 286 >B1DC21.1 B1DC21_9BACL MscS Mechanosensitive ion channel Length = 264 Score = 196 bits (497), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 102/219 (46%), Positives = 140/219 (63%), Gaps = 2/219 (0%) Query: 57 KVIDRSMEKESRGRLLGDSRRFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXX 116 K IDR + S R++ +RR +TVG L+KN++++ F++ MLVL EF Sbjct: 19 KTIDRVVLNRSESRMVLQTRRMTTVGKLVKNIISYAGYFIIGMLVLDEFGVQLAPLLAGA 78 Query: 117 XXXXXXXXXXXQSLVKDVITGFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTG 176 QSLVKD+ITGFFIIFEDQFAVGDVIQTG +KGTVE+IGLR TR+LS TG Sbjct: 79 GVIGLAIGFGAQSLVKDIITGFFIIFEDQFAVGDVIQTGEFKGTVEVIGLRATRILSWTG 138 Query: 177 EVHIIPNGTIINVTNYSLANALAVVDVPVKIERGLEATLALIGEALKGIEERNSNVIAYP 236 EVHIIPNG I VTN+S+ N++A +DV + E E + +I L+ E N N++ P Sbjct: 139 EVHIIPNGMINEVTNFSMHNSIAFIDVSIGYEEDAEKAIVIIRRELERFE--NDNLVKPP 196 Query: 237 NILGIQSMSTSEYVIRVAANCLPNARDAAERQIQSDIKR 275 +LGIQ+M ++E +R+ A C+PN + R + + IK+ Sbjct: 197 EVLGIQTMLSTELKVRITAECIPNTQAIVARALNAYIKK 235 >Q8EKV1.1 Q8EKV1_OCEIH Hypothetical conserved protein Length = 323 Score = 175 bits (443), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 88/256 (34%), Positives = 147/256 (57%) Query: 19 DRVWNWVTDAEMWADVLFAGXXXXXXXXXXXXXXKVVYKVIDRSMEKESRGRLLGDSRRF 78 +++WN++++ ++W ++ KV K +D+ + G + RR Sbjct: 9 EKLWNYLSNPDLWLEIFKGALQIVVIILLALVIVKVTRKFVDKFFVQRQTGPIRITERRE 68 Query: 79 STVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXXXXXXXXXXXXXQSLVKDVITGF 138 +T+ L+KNVV ++ F+ I+++L F+F+ Q+LV+D+I+GF Sbjct: 69 NTLKKLIKNVVAYVVYFLAIVMILGVFNFEIGPLLAGAGVAGLAIGFGAQNLVRDIISGF 128 Query: 139 FIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTGEVHIIPNGTIINVTNYSLANAL 198 FIIFEDQF+VGD + +GTVE IGLRTT++LS TGE++++PNG + VTNYS+ N L Sbjct: 129 FIIFEDQFSVGDYVAVSGIEGTVEEIGLRTTKILSWTGELNVVPNGNVTQVTNYSIHNGL 188 Query: 199 AVVDVPVKIERGLEATLALIGEALKGIEERNSNVIAYPNILGIQSMSTSEYVIRVAANCL 258 A+VDV + E + +I E + + ++ P I+G+QS+ S YVIR+ A+ Sbjct: 189 AIVDVNIPYESDVYEAENVIEEVAAELPNKYEFLLEVPQIIGVQSLDVSHYVIRIIADTS 248 Query: 259 PNARDAAERQIQSDIK 274 P + AAER I+ +++ Sbjct: 249 PGFQWAAERNIRKELQ 264 >C0ZA53.1 C0ZA53_BREBN Conserved hypothetical membrane protein Length = 291 Score = 174 bits (442), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 95/259 (36%), Positives = 139/259 (53%) Query: 16 RFKDRVWNWVTDAEMWADVLFAGXXXXXXXXXXXXXXKVVYKVIDRSMEKESRGRLLGDS 75 R +V ++TDA+MW ++ A VV +++ + ++ D Sbjct: 9 RIYAQVHAYMTDADMWVNIGMAVLKIVAIILLSRIVVSVVQSGVNKVFQHRHGSKIQMDQ 68 Query: 76 RRFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXXXXXXXXXXXXXQSLVKDVI 135 RR T+ L+ ++V + F+ I+++L D QSLV+D+I Sbjct: 69 RRVDTMRVLVNHIVQYGIYFLAILMILQLLGIDLRPVLVSAGVLGLAVGFGAQSLVRDII 128 Query: 136 TGFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTGEVHIIPNGTIINVTNYSLA 195 TGFFIIFEDQFAVGDV+ GTV+ IGLR T++ S TGEVHI PNGTI VTN+SL Sbjct: 129 TGFFIIFEDQFAVGDVVTINNLTGTVQEIGLRITKVRSWTGEVHIFPNGTINQVTNFSLQ 188 Query: 196 NALAVVDVPVKIERGLEATLALIGEALKGIEERNSNVIAYPNILGIQSMSTSEYVIRVAA 255 N LAVVDV V E L +++ E L + ++++A P ILG+ ++ SE +IRV A Sbjct: 189 NTLAVVDVSVAYEEDLNQVESVLKEVLASAQTELTDIVAEPQILGVHALGPSEVIIRVTA 248 Query: 256 NCLPNARDAAERQIQSDIK 274 C PN R +++ I+ Sbjct: 249 ECKPNTHHGVNRNLRAKIR 267 >Q2B0Z4.1 Q2B0Z4_9BACI Putative uncharacterized protein Length = 296 Score = 172 bits (435), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 91/252 (36%), Positives = 145/252 (57%) Query: 23 NWVTDAEMWADVLFAGXXXXXXXXXXXXXXKVVYKVIDRSMEKESRGRLLGDSRRFSTVG 82 + ++D MW+ + ++ I + S L RR +T+ Sbjct: 13 DQLSDENMWSAIGLGFLKIIAILIVSSLIIRIGRAAIRKVFAVRSHSPLRISHRREATLV 72 Query: 83 GLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXXXXXXXXXXXXXQSLVKDVITGFFIIF 142 LL+N++T++ +F+MIM++LS D Q+LVKD+ITGFFIIF Sbjct: 73 KLLENILTYVVSFIMIMMILSTLTIDVGALLAGAGIVGLAVGFGAQNLVKDIITGFFIIF 132 Query: 143 EDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTGEVHIIPNGTIINVTNYSLANALAVVD 202 EDQF+VGD I+ ++GTVE IGLRTT++ S TGE+HI+PNG+I+ VTN+SL N++A VD Sbjct: 133 EDQFSVGDYIRVDQFEGTVEEIGLRTTKIKSWTGELHILPNGSIVQVTNFSLHNSVAFVD 192 Query: 203 VPVKIERGLEATLALIGEALKGIEERNSNVIAYPNILGIQSMSTSEYVIRVAANCLPNAR 262 V + E ++ ++ + L+ + E+ +++ P ILGIQ+++ S+ VIRV A +P Sbjct: 193 VSIAYEGDIQKAEQVLKDLLETLPEKYEDMVKTPEILGIQNLAASDVVIRVTAETMPMKH 252 Query: 263 DAAERQIQSDIK 274 RQ++ +IK Sbjct: 253 FFIARQLRKEIK 264 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,407,574,251 Number of extensions: 43235834 Number of successful extensions: 114899 Number of sequences better than 1.0e-05: 850 Number of HSP's gapped: 114741 Number of HSP's successfully gapped: 850 Length of query: 338 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 202 Effective length of database: 1,779,438,163 Effective search space: 359446508926 Effective search space used: 359446508926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)