BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0005.p (60 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5ZYK6.1 A5ZYK6_9FIRM Putative uncharacterized protein 53 9e-06 >A5ZYK6.1 A5ZYK6_9FIRM Putative uncharacterized protein Length = 97 Score = 52.8 bits (125), Expect = 9e-06, Method: Compositional matrix adjust. Identities = 29/40 (72%), Positives = 31/40 (77%), Gaps = 2/40 (5%) Query: 23 FHLQPIYLVVFKG--SYMLGNLILRGASRLDAFSAYPVRT 60 FHL PI LVVFKG S+ +G LILRGASRLDAFS YP R Sbjct: 21 FHLCPIDLVVFKGVYSFRMGYLILRGASRLDAFSVYPFRA 60 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.338 0.152 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 301,100,694 Number of extensions: 8779181 Number of successful extensions: 33744 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 33744 Number of HSP's successfully gapped: 1 Length of query: 60 Length of database: 3,060,696,203 Length adjustment: 32 Effective length of query: 28 Effective length of database: 2,759,223,723 Effective search space: 77258264244 Effective search space used: 77258264244 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 126 (53.1 bits)