BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0003.p (363 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8R8P5.1 Q8R8P5_THETN Predicted dehydrogenases and related proteins 198 6e-49 B7R6U2.1 B7R6U2_9THEO Oxidoreductase family, NAD-binding Rossman... 195 5e-48 B0K7A7.1 B0K7A7_THEP3 Oxidoreductase domain protein 187 2e-45 B0K697.1 B0K697_THEPX Oxidoreductase domain protein 184 9e-45 C0ZD68.1 C0ZD68_BREBN Putative oxidoreductase 177 1e-42 >Q8R8P5.1 Q8R8P5_THETN Predicted dehydrogenases and related proteins Length = 348 Score = 198 bits (504), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 115/356 (32%), Positives = 181/356 (50%), Gaps = 16/356 (4%) Query: 8 KVISIGMIGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAGAKACTSLSE-- 65 K + +GAG VA++ HL G+ L+ V I A+ DPD A LAE+ E Sbjct: 2 KKLRYACVGAGAVADYKHLLGYSRLKDVEIVAISDPDVGAAKRLAEKYSIPHVYENYEEM 61 Query: 66 IDEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLL 125 ++ LD + +CTPN+ H ++ +++GIH+ CEKPMT A+EA + + Sbjct: 62 FEKEKLDLISICTPNYLHAPISIKAMEKGIHIHCEKPMTLNAKEAHKVIEAKNKYKTKFM 121 Query: 126 MGFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDL 185 +G NR+ ++ ++ I G +G++ + WRR++GIP G WFT +K+SGGG LIDL Sbjct: 122 VGLNNRFTNESYFVKKYIEEGYLGEIYHVKTGWRRRRGIPGKGGWFTTKKLSGGGPLIDL 181 Query: 186 GSHMIDQMLFLTDAPEPVACLAGLHSRPAQEGDYSNWLGSNESVTL-EVEDTAIGMIQFA 244 G H ID +++ D PE + A +S+ + ++ W + + VED A+G I+ Sbjct: 182 GVHFIDLVMYFMDYPEVKSVSAATYSKFSNSTSWNGWSEAKSGNGIYNVEDMAVGFIRLK 241 Query: 245 NGMLGRIHVSWQDDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQEPE 304 NG SW ++EGD+ + G +G + F N + + T P Sbjct: 242 NGTTIDFEFSWASNIEGDYNYYEILGDKGGI------WFKNGQIKVFSEVVNTLVDIIPN 295 Query: 305 WFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKGL 360 +P K L +F HF CI+E K+P +AE+G K + +I Y SA KG+ Sbjct: 296 -TNYPKKP--LDEFE----HFVNCIKEGKEPLASAEEGAKLMEIIDAAYLSAEKGV 344 >B7R6U2.1 B7R6U2_9THEO Oxidoreductase family, NAD-binding Rossmann fold protein Length = 348 Score = 195 bits (496), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 114/356 (32%), Positives = 181/356 (50%), Gaps = 16/356 (4%) Query: 8 KVISIGMIGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAGAKACTSLSE-- 65 K + +GAG VA++ HL G+ L+ V I A+ DPD A LAE+ E Sbjct: 2 KKLRYACVGAGAVADYKHLLGYSRLKDVEIVAISDPDVGAAKRLAEKYSISHVYENYEEM 61 Query: 66 IDEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLL 125 ++ LD + +CTPN+ H ++ +++GI+V CEKPMT A+EA + + Sbjct: 62 FEKEKLDLISICTPNYLHAPISIKAVEKGIYVHCEKPMTLNAKEAHKVIEAKNKYKTKFM 121 Query: 126 MGFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDL 185 +G NR+ ++ ++ I G +G++ + WRR++GIP G WFT +++SGGG LIDL Sbjct: 122 VGLNNRFTNESYFVKEYIEEGYLGEIYHVKTGWRRRRGIPGKGGWFTTKELSGGGPLIDL 181 Query: 186 GSHMIDQMLFLTDAPEPVACLAGLHSRPAQEGDYSNWLGSNESVTL-EVEDTAIGMIQFA 244 G H ID +++ D PE + A +S+ + ++ W + + VED A+G I+ Sbjct: 182 GVHFIDLVMYFMDYPEVKSVSAATYSKFSNSTSWNGWSEAKSGNGIYNVEDMAVGFIRLK 241 Query: 245 NGMLGRIHVSWQDDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQEPE 304 NG SW ++EGD+ + G +G + F N + + T P Sbjct: 242 NGTTIDFEFSWASNIEGDYNYYEILGDKGGI------WFKNGQIKVFSEVVNTLVDIIPN 295 Query: 305 WFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKGL 360 +P K L +F HF CI+E K+P +AE+G K + +I Y SA KG+ Sbjct: 296 -TNYPKKP--LDEFE----HFVNCIKEGKEPLASAEEGAKLMEIIDAAYLSAEKGV 344 >B0K7A7.1 B0K7A7_THEP3 Oxidoreductase domain protein Length = 349 Score = 187 bits (474), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 115/349 (32%), Positives = 184/349 (52%), Gaps = 18/349 (5%) Query: 15 IGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAG-AKACTSLSEIDEIPL-D 72 +GAG VA++ HL G+ +EGV I A+ DPD A LAE+ T E+ E + Sbjct: 9 VGAGSVADYKHLNGYSKVEGVEIVAISDPDIEAAKRLAEKYNIPHVYTDYEEMFEREEPN 68 Query: 73 AVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLLMGFVNRY 132 + VCTPN+ H ++ +++GIHV CEKP+T +A+EA + ++G NR+ Sbjct: 69 LISVCTPNYLHAPISIKAMEKGIHVHCEKPITLSAKEAYEVIESKNKYKRKFMIGLNNRF 128 Query: 133 RDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDLGSHMIDQ 192 ++ ++ I G IG++ + WRR++GIP G WFT +K+SGGG LIDLG H +D Sbjct: 129 TNESFFVKRYIEEGYIGEIYHVKTGWRRRRGIPGKGGWFTNKKLSGGGPLIDLGVHFLDL 188 Query: 193 MLFLTDAPEPVACLAGLHSR--PAQEGDYSNWLGSNESVTLEVEDTAIGMIQFANGMLGR 250 +L+ PE + A +S+ + + N+ S + + +VED A+G ++ NG Sbjct: 189 VLYFMGYPEVQSVSAATYSKFSNSNSLNSWNYAKSGDGI-YDVEDMAVGFVRLKNGATID 247 Query: 251 IHVSWQDDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQEPEWFTFPP 310 SW ++EGD+ + G +G + F N L + T P+ T P Sbjct: 248 FEFSWASNIEGDYNHYEILGDKGGI------WFKNGQLKVFSEIVETLIEIVPD--TNYP 299 Query: 311 KTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKG 359 K+ + + HF +CI+ K+P +AE+G K +++I Y+SA KG Sbjct: 300 KSPL-----NEFEHFVDCIKNDKEPLASAEEGAKLMKIIDGAYESAEKG 343 >B0K697.1 B0K697_THEPX Oxidoreductase domain protein Length = 349 Score = 184 bits (468), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 116/350 (33%), Positives = 182/350 (52%), Gaps = 20/350 (5%) Query: 15 IGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAG-AKACTSLSEI--DEIPL 71 +GAG VA++ HL G+ +E V I A+ DPD A LAE+ T E+ E P Sbjct: 9 VGAGSVADYKHLNGYSKVEDVEIVAISDPDIEAAKRLAEKYNIPHVYTDYEEMFKKEKP- 67 Query: 72 DAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLLMGFVNR 131 D + VCTPN+ H ++ +++GIHV CEKP+T A+EA + ++G NR Sbjct: 68 DLISVCTPNYLHAPISIKAMEKGIHVHCEKPITLNAKEAYEVIESKNKYKRKFMVGLNNR 127 Query: 132 YRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDLGSHMID 191 + ++ ++ I G IG++ + WRR++GIP G WFT +K+SGGG LIDLG H +D Sbjct: 128 FTNESFFVKRYIEEGYIGEIYHVKTGWRRRRGIPGKGGWFTNKKLSGGGPLIDLGVHFLD 187 Query: 192 QMLFLTDAPEPVACLAGLHSR--PAQEGDYSNWLGSNESVTLEVEDTAIGMIQFANGMLG 249 +L+ PE + A +S+ + + N+ S + + +VED A+G ++ NG Sbjct: 188 LVLYFMGYPEVQSVSAATYSKFSNSNSLNSWNYAKSGDGI-YDVEDMAVGFVRLKNGATI 246 Query: 250 RIHVSWQDDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQEPEWFTFP 309 SW ++EGD+ + G +G + F N L + T P+ T Sbjct: 247 DFEFSWASNIEGDYNYYEILGDKGGI------WFKNGQLKVFSEIVDTLIDIVPD--TNY 298 Query: 310 PKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKG 359 PK+ + + HF +CI+ K+P +AE+G K + +I Y+SA KG Sbjct: 299 PKSPL-----NEFEHFVDCIKNDKEPLASAEEGAKLMGIIDGAYESAEKG 343 >C0ZD68.1 C0ZD68_BREBN Putative oxidoreductase Length = 352 Score = 177 bits (449), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 116/346 (33%), Positives = 176/346 (50%), Gaps = 28/346 (8%) Query: 24 GHLPGFLSLEGVSIGAVYDPDASRAAHLAE-----QAGAKACTSLSEIDEIPLDAVLVCT 78 HL + A+ D A A++ A+ Q T L E D +DA++VC Sbjct: 20 AHLEAIKEESRAEVVAIADISAEMASYRAKRHDIPQVFTDYETMLQESD---VDAIIVCV 76 Query: 79 PNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLLMGFVNRYRDDIRE 138 PN+ H E KH L G H+LCEKPM A+EA + A+ + +L++ NR+R D + Sbjct: 77 PNYLHAEATKHALAAGKHLLCEKPMAMNAQEAEEMIEAAKKADKILMVAQNNRFRSDSLQ 136 Query: 139 LRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDLGSHMIDQMLFLTD 198 +++ + G++G++ + W R+ GIP GSWFTQ++ +GGG LID+G HM+D L+L D Sbjct: 137 VKTWLKEGKLGNIYHAKTGWVRRNGIPGWGSWFTQKESAGGGPLIDIGVHMLDLTLWLLD 196 Query: 199 APEPVACLAGLHSR--PAQEGDYSNWLGSNESVTLEVEDTAIGMIQFANGMLGRIHVSWQ 256 P+PV+ L +++ P + G S W +++ +VED A+ MI F NG+ + SW Sbjct: 197 HPKPVSVLGQTYAQFGPHKRG-LSEWGRIDDNGKFDVEDMAVAMITFENGLSLTLDASWA 255 Query: 257 DDVEGDFVEIHLKGSRGSLSLRTL---FGFSNQGLYKQPSLQFTPAGQEPEWFTFPPKTD 313 +E + V + L G G SLR L F ++ S + TP P K Sbjct: 256 SHIEEENVFLQLYGQEGGASLRLLENQFTLYHEWNGVTASTELTPK---------PQKER 306 Query: 314 VLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKG 359 VL+ FR +F + I + T E RLI+ +Y SA KG Sbjct: 307 VLL-FR----NFIDAIEGKAEVLCTPEQALYINRLIEAIYTSAQKG 347 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,263,876,872 Number of extensions: 95893560 Number of successful extensions: 217694 Number of sequences better than 1.0e-05: 4816 Number of HSP's gapped: 213599 Number of HSP's successfully gapped: 4816 Length of query: 363 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 226 Effective length of database: 1,770,017,148 Effective search space: 400023875448 Effective search space used: 400023875448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)