BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0001.p (381 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8LCS9.1 A8LCS9_FRASN Glutamine--scyllo-inositol transaminase EC... 322 5e-86 Q0RS85.1 Q0RS85_FRAAA 3-amino-5-hydroxybenzoic acid synthase 320 2e-85 Q2JFX6.1 Q2JFX6_FRASC DegT/DnrJ/EryC1/StrS aminotransferase 319 2e-85 Q44131.1 Q44131_ACTPA 3-amino-5-hydroxybenzoic acid synthase 315 6e-84 C0TCP3.1 C0TCP3_9PSEU Predicted PLP-dependent enzyme possibly in... 313 2e-83 >A8LCS9.1 A8LCS9_FRASN Glutamine--scyllo-inositol transaminase EC=2.6.1.50 Length = 388 Score = 322 bits (824), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 168/359 (46%), Positives = 220/359 (61%), Gaps = 4/359 (1%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWRM G +V FER FAE H ++YAL VTNGT+A++L L+ V G EVIVP FTFI Sbjct: 32 GQWWRMGGGEVDAFEREFAEYHGSRYALAVTNGTHALELALQVLGVGPGTEVIVPGFTFI 91 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ LGA+ VPVD+D T+C+DP V A+T RT I+PVHMAGHL DMD L I+ Sbjct: 92 SSSQAAQRLGAVAVPVDVDLDTYCIDPAAVETAITPRTKAIMPVHMAGHLSDMDALAKIS 151 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 + + I++DA HAHG +W+G++ G G FSFQ KLMTAGEGGA+ ELY+QA Sbjct: 152 ANTGVPIIQDAAHAHGTQWQGRKVGEHGSVAAFSFQNGKLMTAGEGGAVTFPDAELYEQA 211 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 FL H+ GRP D++Y H G+N+R +EF A+VL Q RL Q RE+ +L+ + Sbjct: 212 FLRHSCGRPRTDRRYFHQTSGSNFRMNEFSASVLRAQLARLGGQIDTREQRWPVLSGLLA 271 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHDT 316 I G+VPQ R C ++HYM MF PG FG R V+ L E+G+PA+ A+ I+ + Sbjct: 272 EIPGVVPQSRDPRCDRNSHYMAMFRV-PG-FGEERRNALVDALIERGLPAFAAFRSIYRS 329 Query: 317 PIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEEATRGIAQTIREIQA 375 F E A D CP + ++ + IW+HHR LLG EE IA I E A Sbjct: 330 DGFWETGAP--DETVDQIAARCPNVEALSADGIWLHHRTLLGTEEQMHDIAAIIAETLA 386 >Q0RS85.1 Q0RS85_FRAAA 3-amino-5-hydroxybenzoic acid synthase Length = 391 Score = 320 bits (819), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 161/359 (44%), Positives = 223/359 (62%), Gaps = 4/359 (1%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWRM G++V FER FA+ H A YAL VTNGT+A++L L+ V G EVIVPAFTFI Sbjct: 35 GQWWRMGGSEVDSFEREFADYHGADYALAVTNGTHALELALQVLGVGPGTEVIVPAFTFI 94 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ LGA+ VPVD+D T+C+DP AVT RT I+PVHMAG + DMD L ++ Sbjct: 95 SSSQAAQRLGAVAVPVDVDLDTYCIDPAAAAAAVTPRTKAIMPVHMAGQVSDMDALAKLS 154 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 ++ + +++DA HAHG +W+G++ G +G FSFQ KLMTAGEGGA+ EL++ A Sbjct: 155 ADTGVPLIQDAAHAHGAQWQGRKVGELGSVAAFSFQNGKLMTAGEGGAVTFPDSELHEIA 214 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 FL H+ GRP D++Y H G+N+R +EF A+VL Q GRLA Q RE+ +L E + Sbjct: 215 FLRHSCGRPRTDRRYFHQTSGSNFRLNEFSASVLRAQLGRLAGQIDTREQRWPVLAEALS 274 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHDT 316 I G+VPQ R E C + HYM MF PG F R V+ L E+G+PA+ A+ I+ + Sbjct: 275 AIPGVVPQSRDERCDRNPHYMAMFRL-PG-FSEQRRNAVVDALVERGLPAFAAFRAIYRS 332 Query: 317 PIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEEATRGIAQTIREIQA 375 F E A + + CP ++ ++ + +W+HHR LLG EE +A + + A Sbjct: 333 NGFWETGAPAESVDDIARR--CPNTEALSADGVWLHHRTLLGSEEQMHEVAAVVADTLA 389 >Q2JFX6.1 Q2JFX6_FRASC DegT/DnrJ/EryC1/StrS aminotransferase Length = 388 Score = 319 bits (818), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 165/362 (45%), Positives = 223/362 (61%), Gaps = 10/362 (2%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWRM G++V FER FA H A YAL VTNGT+A++L L+ V G EVIVPAFTFI Sbjct: 32 GQWWRMGGSEVDAFEREFAAYHGAGYALAVTNGTHALELALQVLGVGPGSEVIVPAFTFI 91 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ LGA+ VPVDIDPGT+C+ AVT RT I+PVHMAG +CDMD L ++ Sbjct: 92 SSSQAAQRLGAVAVPVDIDPGTYCIGVTAAAAAVTPRTRAIMPVHMAGQVCDMDALAKLS 151 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 ++ + +++DA HAHG +W+GK+ G +G FSFQ KLMTAGEGGA+ ELY+ Sbjct: 152 ADAGVPLIQDAAHAHGAQWQGKKLGELGSVAAFSFQNGKLMTAGEGGAVTFPDAELYELG 211 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 FL H+ GRP D++Y H G+N+R +EF AAVL Q GRLA Q RE +L + Sbjct: 212 FLRHSCGRPRTDRRYLHQTSGSNFRLNEFSAAVLRAQLGRLAGQIDTREERWPVLAGALA 271 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRER---FVEMLNEQGIPAYIAYAQI 313 I G+VPQGR + C + HYM MF + G S ER FV+ L ++G+PA+ A+ I Sbjct: 272 DIPGVVPQGRDDRCDRNPHYMAMF-----RLPGFSEERRNAFVDALVDRGLPAFAAFRAI 326 Query: 314 HDTPIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEEATRGIAQTIREI 373 + + F E A + + CP ++ ++ + +W+HHR LLG EE +A + E Sbjct: 327 YRSNGFWETGAPDETVDEIAKR--CPNTEAISADGVWLHHRTLLGTEEQMHQVAAIVAET 384 Query: 374 QA 375 A Sbjct: 385 LA 386 >Q44131.1 Q44131_ACTPA 3-amino-5-hydroxybenzoic acid synthase Length = 388 Score = 315 bits (806), Expect = 6e-84, Method: Compositional matrix adjust. Identities = 160/353 (45%), Positives = 220/353 (62%), Gaps = 14/353 (3%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWR+ G++V EFER FAE H A +AL VTNGT+A++L L+ V G EVIVPAFTFI Sbjct: 32 GQWWRVGGSEVDEFEREFAEYHGAGHALAVTNGTHALELALQVLDVGPGTEVIVPAFTFI 91 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ + LGA+ VPVD+DP T+CLD +AVT RT I+PVHMAG DMDRL ++ Sbjct: 92 SSSQAVQRLGAVAVPVDVDPDTYCLDVAAAEDAVTSRTSAIMPVHMAGQFADMDRLDKLS 151 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 + + +++DA HAHG W+GKR G +G FSFQ KLMTAGEGGA++ Q +++A Sbjct: 152 ASTGVPVVQDAAHAHGAHWRGKRVGELGSIATFSFQNGKLMTAGEGGAVLFADQAQWEKA 211 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 F+ H+ GRP GD+ Y HL G+N+R +EF AAVL Q GRL Q R+ +L+ + Sbjct: 212 FVLHSCGRPKGDRGYFHLTSGSNFRMNEFSAAVLRAQLGRLDSQIATRQARWPVLSALLA 271 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRER---FVEMLNEQGIPAYIAYAQI 313 I+G+VPQ + YM MF + G++ ER V+ L +GIPA++A+ + Sbjct: 272 GIDGVVPQTVDPRSDRNPSYMAMF-----RMPGVTEERRNAVVDELVRRGIPAFMAFRAV 326 Query: 314 HDTPIFKEFAASLDDSYSFPNQL--DCPASKKVAEEVIWIHHRALLGDEEATR 364 + T F E A P +L CP S+++ + +W+HHR LLG EE R Sbjct: 327 YRTQAFWETGA----PDLTPEELAARCPVSEEITRDCVWLHHRVLLGAEEQVR 375 >C0TCP3.1 C0TCP3_9PSEU Predicted PLP-dependent enzyme possibly involved in cell wall biogenesis Length = 388 Score = 313 bits (801), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 157/348 (45%), Positives = 217/348 (62%), Gaps = 10/348 (2%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWR+ G++V EFER FAE H A +AL VTNGT+A++L L+ V G EVIVPAFTFI Sbjct: 32 GQWWRVGGSEVDEFEREFAEYHGAGHALAVTNGTHALELALQVLDVGPGAEVIVPAFTFI 91 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ + LGA+ VPVD+DP T+CLD AVT RT I+PVHMAG DMDRL ++ Sbjct: 92 SSSQAVQRLGAVAVPVDVDPDTYCLDVAAAEAAVTSRTSAIMPVHMAGQFADMDRLDKLS 151 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 + + +++DA HAHG W+GKR G +G FSFQ KLMTAGEGGA++ + +++A Sbjct: 152 TSTGVPVLQDAAHAHGAHWRGKRVGELGSIATFSFQNGKLMTAGEGGAVLFEDEAQWEKA 211 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 F+ H+ GRP GD+ Y HL G+N+R +EF AAVL Q GRL Q R+ +L+ + Sbjct: 212 FVLHSCGRPKGDRGYFHLTSGSNFRMNEFSAAVLRAQLGRLDSQIATRQARWPVLSALLA 271 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRER---FVEMLNEQGIPAYIAYAQI 313 I+G+VPQ + YM MF + G++ ER V+ L +GIPA++A+ + Sbjct: 272 GIDGVVPQTVDPRSDRNPSYMAMF-----RMPGVTEERRNAVVDELVRRGIPAFMAFRAV 326 Query: 314 HDTPIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEE 361 + T F E A D + CP S+++ + +W+HHR LLG EE Sbjct: 327 YRTQAFWETGAP--DLTTEELAARCPVSEEITRDCVWLHHRVLLGTEE 372 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,238,001,064 Number of extensions: 96048239 Number of successful extensions: 230721 Number of sequences better than 1.0e-05: 3115 Number of HSP's gapped: 226088 Number of HSP's successfully gapped: 3120 Length of query: 381 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 244 Effective length of database: 1,770,017,148 Effective search space: 431884184112 Effective search space used: 431884184112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)