BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0012.p (279 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2B899.1 Q2B899_9BACI Putative LPS biosynthesis related reductase 295 4e-78 C3WC55.1 C3WC55_FUSMR dTDP-4-dehydrorhamnose reductase 232 2e-59 A8RK68.1 A8RK68_9CLOT Putative uncharacterized protein 223 2e-56 A6L9I5.1 A6L9I5_PARD8 Putative reductase 219 3e-55 B1HRS5.1 B1HRS5_LYSSC Putative reductase 214 6e-54 >Q2B899.1 Q2B899_9BACI Putative LPS biosynthesis related reductase Length = 275 Score = 295 bits (754), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 142/275 (51%), Positives = 194/275 (70%), Gaps = 2/275 (0%) Query: 1 MKLLILGGNGMAGHMLADYFRRQGKHHVFHTTRDKSDLGGLYIDADDIAGVEKLVEIVSP 60 MK+LILGG GMAGHM+ Y R + VF TTRD++D G+Y+DA D +EK+++ P Sbjct: 1 MKILILGGRGMAGHMMVAYLRENTDYQVFFTTRDRADREGIYLDAADSPMLEKVIDQHRP 60 Query: 61 HCIINALGVLN-QFAERDRIGAYHVNGFLPHRLRRAADGVHARLIHISTDCVFEGTRGGY 119 IIN +G+LN Q AE ++ A+ +NG LPH++ R A+ RLIHISTDCVF G G Y Sbjct: 61 DIIINCIGLLNGQAAENPKL-AFQLNGLLPHQIARFAERYGGRLIHISTDCVFSGGIGSY 119 Query: 120 TEDDTPDGTSVYAITKSLGEVREPGHLTIRTSIIGPEIRPSGIGLMEWFLAQRGPVSGYR 179 E+DTPDGTS+YA +K LGE+ + HLT+RTSIIGPE++ GIGL WF+ Q+G ++GY Sbjct: 120 KEEDTPDGTSIYAQSKQLGEIVDSRHLTVRTSIIGPELKDDGIGLFLWFMKQQGRINGYT 179 Query: 180 RVMWNGVTTLELAKAIDGLLEPEISGLIHLAHPQPVSKYELLRHMQAAFNKEDVEIIPES 239 +WNGVTTLELAKA+ G++E E+SGL HL + +SK++LL +A FNK+ V+I+P+S Sbjct: 180 EALWNGVTTLELAKAVRGMIETELSGLCHLGGERKISKHDLLVLFKAIFNKDGVDIVPDS 239 Query: 240 AHIQDRTLVSTRADVQVQLPPYPVMLAELAAWMKQ 274 + DRT+V+TR D +P Y M+ EL WM + Sbjct: 240 GFVLDRTIVNTRTDFDYHVPGYDEMILELKDWMDR 274 >C3WC55.1 C3WC55_FUSMR dTDP-4-dehydrorhamnose reductase Length = 274 Score = 232 bits (592), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 112/273 (41%), Positives = 167/273 (61%), Gaps = 5/273 (1%) Query: 2 KLLILGGNGMAGHMLADYFRRQGKHHVFHTTRDKSDLGG---LYIDADDIAGVEKLVEIV 58 K+LI+G GMAG + Y +G + V+ T R ++DA D + K+++ V Sbjct: 4 KILIIGSKGMAGQTIRKYLLSKG-YEVYCTFRKGEAEKKEKEFHLDAFDKEELNKILKEV 62 Query: 59 SPHCIINALGVLNQFAERDRIGAYHVNGFLPHRLRRAADGVHARLIHISTDCVFEGTRGG 118 P+ +IN +G+LNQFAE + A +VNG+ PH L R + + +LIHI+TDCVF G +G Sbjct: 63 KPNFVINCVGILNQFAEENPDIAIYVNGYFPHYLDRLSKEYNYKLIHITTDCVFSGKKGN 122 Query: 119 YTEDDTPDGTSVYAITKSLGEVREPGHLTIRTSIIGPEIRPSGIGLMEWFLAQRGPVSGY 178 Y E+D D S Y +K++GEV LT RTSI+GP+ +GIGL +WF Q G + GY Sbjct: 123 YVENDFRDADSYYGRSKAIGEVNNDRTLTFRTSIVGPDENENGIGLFKWFTKQEGKIKGY 182 Query: 179 RRVMWNGVTTLELAKAIDGLLEPEISGLIHLAHPQPVSKYELLRHMQAAFNKEDVEIIPE 238 V W+GVTTLELAKAI+ E ++GL HL + + +SKYELL+ + A + K+D+EI Sbjct: 183 SNVFWSGVTTLELAKAIEASFETNLTGLYHLVNNKKISKYELLK-LFAKYAKKDIEIEEY 241 Query: 239 SAHIQDRTLVSTRADVQVQLPPYPVMLAELAAW 271 + D++L++TR D ++ Y M+ E+ + Sbjct: 242 KDYYSDKSLINTRKDFNYKVSSYEKMIEEMVKF 274 >A8RK68.1 A8RK68_9CLOT Putative uncharacterized protein Length = 299 Score = 223 bits (567), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 110/278 (39%), Positives = 167/278 (60%), Gaps = 10/278 (3%) Query: 1 MKLLILGGNGMAGHMLADYFRRQGKHHVFHTTRDKSDLGGLYIDA--DDIAGVEKLVEIV 58 M+ LILG NGMAG+ +A Y + G H V R+KS LY D+ D +L E++ Sbjct: 16 MRFLILGSNGMAGNTIAVYLKETG-HEVLGYAREKS----LYFDSVIGDAYNTVQLKEVI 70 Query: 59 SP---HCIINALGVLNQFAERDRIGAYHVNGFLPHRLRRAADGVHARLIHISTDCVFEGT 115 IIN +G+LNQFAE ++ GA +N +LPH L +G +++H+STDCVF G Sbjct: 71 DSGNFDSIINCVGLLNQFAENNKPGAVFLNSYLPHYLAGITEGTSIQVVHMSTDCVFSGE 130 Query: 116 RGGYTEDDTPDGTSVYAITKSLGEVREPGHLTIRTSIIGPEIRPSGIGLMEWFLAQRGPV 175 +G Y E D PDG + Y TK+LGE+ + ++T+R SI+GP+I+ SGIGL+ WF+ Q G + Sbjct: 131 KGNYLESDFPDGKTFYDRTKALGELNDDKNITLRNSIVGPDIKASGIGLLSWFMKQSGTI 190 Query: 176 SGYRRVMWNGVTTLELAKAIDGLLEPEISGLIHLAHPQPVSKYELLRHMQAAFNKEDVEI 235 +G+ + MW G TT++LAK ++ + + + GL + +SKYELL+ V I Sbjct: 191 NGFTKAMWTGQTTVQLAKTMEYVAKERMGGLYNAVPHNSISKYELLKLFNKYLRDNSVTI 250 Query: 236 IPESAHIQDRTLVSTRADVQVQLPPYPVMLAELAAWMK 273 P + D++LV ++ ++P Y M+AE+A WM+ Sbjct: 251 NPVEGIVADKSLVPSKLGDDFEIPNYEQMVAEMALWMR 288 >A6L9I5.1 A6L9I5_PARD8 Putative reductase Length = 282 Score = 219 bits (557), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 109/276 (39%), Positives = 165/276 (59%), Gaps = 8/276 (2%) Query: 1 MKLLILGGNGMAGHMLADYFRRQGKHHVFHTTRDK----SDLGGLYIDADDIAGVEKLVE 56 MK+L+LG +GMAGH +A YF+ QG + V+ +R + + +GG DA DI ++ +V Sbjct: 1 MKILVLGASGMAGHTIALYFKEQG-YEVYAFSRKRFPFCTWIGG---DAFDIECLKNIVT 56 Query: 57 IVSPHCIINALGVLNQFAERDRIGAYHVNGFLPHRLRRAADGVHARLIHISTDCVFEGTR 116 + IIN +G+LNQFA+ A ++N +LPH++ R I +STDCVF G + Sbjct: 57 TGNYDAIINCIGLLNQFADSAPEKAVYINSYLPHQVVAWLKDTPTRFIQMSTDCVFAGNK 116 Query: 117 GGYTEDDTPDGTSVYAITKSLGEVREPGHLTIRTSIIGPEIRPSGIGLMEWFLAQRGPVS 176 G Y E PDG S Y TK+LGE+ + +LT R SIIGP+I GIGL WF+ Q P++ Sbjct: 117 GPYDECSVPDGCSYYDRTKALGEIVDDKNLTFRNSIIGPDINEHGIGLFHWFMKQHTPIN 176 Query: 177 GYRRVMWNGVTTLELAKAIDGLLEPEISGLIHLAHPQPVSKYELLRHMQAAFNKEDVEII 236 G+ + +W GVTTL LAKA++ L+ ++GL +L + ++KY LL F +V+I Sbjct: 177 GFTKAIWTGVTTLTLAKAMERALQTNLTGLYNLVNNGSINKYNLLCLFNKYFRNNEVQIA 236 Query: 237 PESAHIQDRTLVSTRADVQVQLPPYPVMLAELAAWM 272 + D+TL++ R D ++P Y M+ E+ W+ Sbjct: 237 KSEKLVLDKTLLNKRTDFDFEVPSYEQMIIEMKEWV 272 >B1HRS5.1 B1HRS5_LYSSC Putative reductase Length = 284 Score = 214 bits (545), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 109/277 (39%), Positives = 157/277 (56%), Gaps = 6/277 (2%) Query: 1 MKLLILGGNGMAGHMLADYFRRQGKHHVFHTTRDKSDLGGLYIDADDIAGVEKLVEIVSP 60 MK L+LG +GMAGH ++ Y R QG H V TR G DI + L ++ Sbjct: 1 MKFLVLGASGMAGHTISIYLREQG-HDVTTYTRTAFPFGQNI--TGDITDLNFLCSLLQD 57 Query: 61 H---CIINALGVLNQFAERDRIGAYHVNGFLPHRLRRAADGVHARLIHISTDCVFEGTRG 117 H ++N +G+LN+ E + + +N +LPH + + +LIH+STDCVF G Sbjct: 58 HHYDIVVNCIGILNKACEENPEKSIFLNSYLPHAIVSLLENRQTKLIHMSTDCVFSGENA 117 Query: 118 GYTEDDTPDGTSVYAITKSLGEVREPGHLTIRTSIIGPEIRPSGIGLMEWFLAQRGPVSG 177 Y E DGT+ Y TK LGE+ HLT R SIIGP+++ GIGL WF+ Q+GP++G Sbjct: 118 PYYEKSVQDGTTFYDRTKGLGEINHKKHLTFRNSIIGPDLKKDGIGLFNWFMHQKGPING 177 Query: 178 YRRVMWNGVTTLELAKAIDGLLEPEISGLIHLAHPQPVSKYELLRHMQAAFNKEDVEIIP 237 Y +W GVTTL LAKAI+ + ISGL HL + P+SKY+LL+ + F ++I P Sbjct: 178 YTGAIWTGVTTLTLAKAIEQAAQEGISGLYHLVNTLPISKYDLLQLLNKHFMDHTIKITP 237 Query: 238 ESAHIQDRTLVSTRADVQVQLPPYPVMLAELAAWMKQ 274 ++TL++TR D +P Y M+ ++ W+ Q Sbjct: 238 HPLVNVNKTLINTRNDFSFTVPSYEEMIIDMKEWILQ 274 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,780,155,687 Number of extensions: 77298255 Number of successful extensions: 180744 Number of sequences better than 1.0e-05: 532 Number of HSP's gapped: 180838 Number of HSP's successfully gapped: 532 Length of query: 279 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 145 Effective length of database: 1,798,280,193 Effective search space: 260750627985 Effective search space used: 260750627985 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)