BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0011.p (333 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2B898.1 Q2B898_9BACI UDP-2-acetamido-2,6-dideoxy-hexulose 4-red... 202 5e-50 Q81A43.1 Q81A43_BACCR UDP-2-acetamido-2,6-dideoxy-hexulose 4-red... 185 6e-45 B5UKH6.1 B5UKH6_BACCE UDP-2-acetamido-2,6-dideoxy-hexulose 4-red... 184 1e-44 B7HDM6.1 B7HDM6_BACC4 UDP-2-acetamido-2,6-dideoxy-hexulose 4-red... 183 2e-44 A9VRZ2.1 A9VRZ2_BACWK NAD-dependent epimerase/dehydratase 181 8e-44 >Q2B898.1 Q2B898_9BACI UDP-2-acetamido-2,6-dideoxy-hexulose 4-reductase Length = 312 Score = 202 bits (513), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 108/237 (45%), Positives = 149/237 (62%), Gaps = 11/237 (4%) Query: 4 RRILITGASGFTGRHAVAYFHAAGXXXXXXXXXXXXXSGIFPAGVKQQVCDLSDRRAVQS 63 +RILITGASGFTGRHA +YF G + ++C+L D+ ++ Sbjct: 5 KRILITGASGFTGRHACSYFRKKGFFTAASVHRGNLQE---SEADQIEICNLLDKNSLDQ 61 Query: 64 MIEEVEPEEVLHLAGKNSVPESWQDPLLYMETNVMSTLYLLEGLRT-RPARRILVAGSRL 122 ++++ +P VLHLA +N V +SW+DP E NV+STLYLLE LR +P R+LV GS L Sbjct: 62 LVKKTDPGYVLHLAAQNHVGKSWEDPSSSFEANVLSTLYLLEALRRLKPEARVLVTGSAL 121 Query: 123 KFKPGLAEGPPHPYSLSKTLEELVSLAWCTLFKQPVLLAEPCNLIGPGPSTGFCSLLAQH 182 + +E PHPYSL+K+++ ++ AW +LFK V++A+PCNLIGPG S G CSL A Sbjct: 122 Q---SCSEDHPHPYSLTKSMQTSLARAWESLFKMDVMIAKPCNLIGPGNSNGVCSLFASR 178 Query: 183 IVRSEAAAGTSETLPPFRLSSRFALRDFLDVRDAVRAYDCILRSGEPGKVYRIDSGT 239 I R E +G E + ++S + RDFLDVRDAVRAY IL GE G++Y + SG+ Sbjct: 179 IARME--SGLEEKV--LYVNSLQSSRDFLDVRDAVRAYGFILDKGERGEIYDVSSGS 231 >Q81A43.1 Q81A43_BACCR UDP-2-acetamido-2,6-dideoxy-hexulose 4-reductase Length = 303 Score = 185 bits (469), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 117/327 (35%), Positives = 166/327 (50%), Gaps = 32/327 (9%) Query: 2 KTRRILITGASGFTGRHAVAYFHAAGXXXXXXXXXXXXXSGIFPAGVKQQVCDLSDRRAV 61 K +LITGA+GFTGRHA YF G I G+ C+L+++ V Sbjct: 3 KNASLLITGANGFTGRHACHYFLEQGFHVIPMFQNRAHREKI-RNGI---TCNLTNKSEV 58 Query: 62 QSMIEEVEPEEVLHLAGKNSVPESWQDPLLYMETNVMSTLYLLEGLRTRPAR-RILVAGS 120 ++++++P+ VLHLAG+NSV ESW L Y+E NV+ TLYLLE ++ + R LV GS Sbjct: 59 MKVMKQIKPDYVLHLAGRNSVIESWTAALEYIEINVIGTLYLLEAIKQEASHCRTLVIGS 118 Query: 121 RLKFKPGLAEGPPHPYSLSKTLEELVSLAWCTLFKQPVLLAEPCNLIGPGPSTGFCSLLA 180 L+ +PYSLSKT++ +++ AW L +++A+P NLIGPG S G CS+LA Sbjct: 119 ALQADSMNEMKISNPYSLSKTMQVIIAEAWGGLMDSNIIIAKPSNLIGPGVSNGICSILA 178 Query: 181 QHIVRSEAAAGTSETLPPFRLSSRFALRDFLDVRDAVRAYDCILRSGEPGKVYRIDSGTL 240 + I+ E +G S+ + ++S RDFLDVRDAV+AY +LR G GK Y I SG Sbjct: 179 KKIIDIE--SGRSKAI--IEVNSLKDSRDFLDVRDAVKAYHVLLRDGINGKQYNIGSGVK 234 Query: 241 RXXXXXXXXXXXXXXXXXXMDWGPVDTXXXXXXXXXXXXXXXXXXXXXXNQEELSASPED 300 R +D + E A+ E Sbjct: 235 RSL---------------------LDVLEQYKELTQLNFFIEETEKSGSDSNESLATEE- 272 Query: 301 NAACLGWSPLIELSRSLADIMDYYRAS 327 LGW P IE +SL D+++Y + S Sbjct: 273 -IKNLGWIPEIEFHQSLKDVLEYAKCS 298 >B5UKH6.1 B5UKH6_BACCE UDP-2-acetamido-2,6-dideoxy-hexulose 4-reductase Length = 303 Score = 184 bits (466), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 116/327 (35%), Positives = 166/327 (50%), Gaps = 32/327 (9%) Query: 2 KTRRILITGASGFTGRHAVAYFHAAGXXXXXXXXXXXXXSGIFPAGVKQQVCDLSDRRAV 61 K +LITGA+GFTGRHA YF G I G+ C+L+++ V Sbjct: 3 KNAILLITGANGFTGRHACHYFLEQGFHVIPMFQNRAHREKI-KNGI---TCNLTNKSEV 58 Query: 62 QSMIEEVEPEEVLHLAGKNSVPESWQDPLLYMETNVMSTLYLLEGLRTRPAR-RILVAGS 120 ++++++P+ VLHLAG+NSV ESW L Y+E NV+ TLYLLE ++ + R LV GS Sbjct: 59 MKVMKQIKPDYVLHLAGRNSVIESWTAALEYIEINVIGTLYLLEAIKQEASHCRTLVIGS 118 Query: 121 RLKFKPGLAEGPPHPYSLSKTLEELVSLAWCTLFKQPVLLAEPCNLIGPGPSTGFCSLLA 180 L+ +PYSLSKT++ +++ AW L +++A+P NLIGPG S G CS+LA Sbjct: 119 ALQANSMNEIKISNPYSLSKTMQVIIAEAWGGLMDSNIIIAKPSNLIGPGVSNGICSILA 178 Query: 181 QHIVRSEAAAGTSETLPPFRLSSRFALRDFLDVRDAVRAYDCILRSGEPGKVYRIDSGTL 240 + ++ E +G S+ + ++S RDFLDVRDAV+AY +LR G GK Y I SG Sbjct: 179 KKMIDIE--SGRSKAI--IEVNSLKDSRDFLDVRDAVKAYHVLLRDGINGKQYNIGSGVK 234 Query: 241 RXXXXXXXXXXXXXXXXXXMDWGPVDTXXXXXXXXXXXXXXXXXXXXXXNQEELSASPED 300 R +D + E A+ E Sbjct: 235 RSL---------------------LDVLEQYKELTQLNFFIEETEKSGSDSNESLATEEI 273 Query: 301 NAACLGWSPLIELSRSLADIMDYYRAS 327 LGW P IE +SL D+++Y + S Sbjct: 274 KK--LGWIPEIEFHQSLKDVLEYAKCS 298 >B7HDM6.1 B7HDM6_BACC4 UDP-2-acetamido-2,6-dideoxy-hexulose 4-reductase Length = 303 Score = 183 bits (464), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 115/327 (35%), Positives = 166/327 (50%), Gaps = 32/327 (9%) Query: 2 KTRRILITGASGFTGRHAVAYFHAAGXXXXXXXXXXXXXSGIFPAGVKQQVCDLSDRRAV 61 K +LITGA+GFTGRHA YF G I G+ C+L+++ V Sbjct: 3 KNASLLITGANGFTGRHACHYFLEQGFHVIPMFQNRAHREKI-RNGI---TCNLTNKSEV 58 Query: 62 QSMIEEVEPEEVLHLAGKNSVPESWQDPLLYMETNVMSTLYLLEGLRTRPAR-RILVAGS 120 ++++++P+ VLHLAG+NSV ESW L Y+E NV+ TLYLLE ++ + R LV GS Sbjct: 59 MKVMKQIKPDYVLHLAGRNSVIESWTAALEYIEINVIGTLYLLEAIKQEASHCRTLVIGS 118 Query: 121 RLKFKPGLAEGPPHPYSLSKTLEELVSLAWCTLFKQPVLLAEPCNLIGPGPSTGFCSLLA 180 L+ +PYSLSKT++ +++ AW L +++A+P NLIGPG S G CS+LA Sbjct: 119 ALQTDSMNEIKISNPYSLSKTMQVIIAEAWGGLMDSNIIIAKPSNLIGPGESNGICSILA 178 Query: 181 QHIVRSEAAAGTSETLPPFRLSSRFALRDFLDVRDAVRAYDCILRSGEPGKVYRIDSGTL 240 + ++ E +G S+ + ++S RDFLDVRDA++AY +LR G GK Y I SG Sbjct: 179 KKMIDIE--SGRSKAI--IEVNSLKDSRDFLDVRDAIKAYHVLLRDGINGKQYNIGSGVK 234 Query: 241 RXXXXXXXXXXXXXXXXXXMDWGPVDTXXXXXXXXXXXXXXXXXXXXXXNQEELSASPED 300 R +D + E A+ E Sbjct: 235 RSL---------------------LDVLEQYKELTQLNFFIEETEKSGSDSNESLATEEI 273 Query: 301 NAACLGWSPLIELSRSLADIMDYYRAS 327 LGW P IE +SL D+++Y + S Sbjct: 274 KK--LGWIPEIEFHQSLKDVLEYAKCS 298 >A9VRZ2.1 A9VRZ2_BACWK NAD-dependent epimerase/dehydratase Length = 302 Score = 181 bits (459), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 101/237 (42%), Positives = 142/237 (59%), Gaps = 9/237 (3%) Query: 6 ILITGASGFTGRHAVAYFHAAGXXXXXXXXXXXXXSGIFPAGVKQQVCDLSDRRAVQSMI 65 +LITGA+GFTGRHA YF G I G+ CDL+++ V +I Sbjct: 7 LLITGANGFTGRHACQYFLEQGFHVIPMFQNRSHREKI-ENGI---TCDLTNKSEVMKVI 62 Query: 66 EEVEPEEVLHLAGKNSVPESWQDPLLYMETNVMSTLYLLEGLRTRPAR-RILVAGSRLKF 124 ++++P+ VLHLAG+NSV ESW L Y+E NV+ TLYLLE ++ + + LV GS L+ Sbjct: 63 KQIKPDYVLHLAGRNSVNESWTASLEYIEINVIGTLYLLEAIKQEASHCKTLVIGSALQA 122 Query: 125 KPGLAEGPPHPYSLSKTLEELVSLAWCTLFKQPVLLAEPCNLIGPGPSTGFCSLLAQHIV 184 +PYSLSKT++ +++ AW L +++A+P NLIGPG S G CS+LA+ ++ Sbjct: 123 DSMKNIKVSNPYSLSKTMQVIIAEAWGGLMDSNIIIAKPSNLIGPGVSNGVCSILAKKMI 182 Query: 185 RSEAAAGTSETLPPFRLSSRFALRDFLDVRDAVRAYDCILRSGEPGKVYRIDSGTLR 241 E +G S+ + ++S RDFLDVRDAV+AY +LR G GK Y I SG R Sbjct: 183 DIE--SGRSKAI--IEVNSLKDSRDFLDVRDAVKAYHVLLRDGINGKQYNIGSGVKR 235 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,685,367,969 Number of extensions: 60920384 Number of successful extensions: 140967 Number of sequences better than 1.0e-05: 1197 Number of HSP's gapped: 140888 Number of HSP's successfully gapped: 1197 Length of query: 333 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 197 Effective length of database: 1,779,438,163 Effective search space: 350549318111 Effective search space used: 350549318111 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)