BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0014.p (387 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DI96.1 B1DI96_9BACL Cell wall hydrolase/autolysin 358 6e-97 C0ZGK0.1 C0ZGK0_BREBN Putative N-acetylmuramoyl-L-alanine amidas... 339 2e-91 Q8ELX9.1 Q8ELX9_OCEIH N-acetylmuramoyl-L-alanine amidase 201 1e-49 Q71ZE9.1 Q71ZE9_LISMF N-acetylmuramoyl-L-alanine amidase, family... 195 7e-48 C1KVG8.1 C1KVG8_LISMC Putative N-acetylmuramoyl-L-alanine amidase 195 7e-48 >B1DI96.1 B1DI96_9BACL Cell wall hydrolase/autolysin Length = 369 Score = 358 bits (918), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 182/386 (47%), Positives = 247/386 (63%), Gaps = 19/386 (4%) Query: 1 MKKTLSTAMLAVTLLLSNPGHPESAHAAGAYTAKVYASSLNVRSEPAASASVTGTLKSGA 60 MKK + +L L+ + P A A+G+YTAKVY SLNVRSEP+ +S+ G LK+G Sbjct: 1 MKKIVYLTILICGLISISGYMPNRAEASGSYTAKVYTDSLNVRSEPSRDSSIVGGLKNGE 60 Query: 61 LVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120 +VTV+ E++GWL++++ VSGWVAG+YLK+ Sbjct: 61 IVTVSAEEYGWLRIKSERVSGWVAGHYLKKVDGNVVTASATDQDGSVRNSSAAARAT--- 117 Query: 121 TVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGDWARIRTTGGEVGWVAGQYIAR 180 V LR+R G G +++LG L +G++VT++ + W R++T ++GWV+ +YIA+ Sbjct: 118 ---VLVDRLRLRAGAGLNHEILGYLTKGEAVTIIDNREGWVRVQTRDKQLGWVSDRYIAK 174 Query: 181 GAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPGHGGSDPGMLGTSYDTMEKDLT 240 G ++GK+IVIDPGHGG DPGM+GT+Y+T+EK+L Sbjct: 175 GETQTVSVASGKSKS-------------LKGKVIVIDPGHGGDDPGMIGTTYETIEKNLN 221 Query: 241 LQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQQLRADAFVSIHYNSSPKKVSG 300 L T+FY+ D LR++GARV +TRT+ +KP LS RV+I + ADAFVSIHYNSS K SG Sbjct: 222 LSTSFYLEDELRSRGARVLMTRTKNEEKPGLSDRVKISESAGADAFVSIHYNSSEKNTSG 281 Query: 301 TLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFGDYHILRENSLPATLVELGFLSNRS 360 LTF+YS D LAR+IENRL GIGLKSNG+S+G+ H+LREN + L+ELGFLSN Sbjct: 282 ILTFYYSNTKDRPLARAIENRLADGIGLKSNGISYGNLHVLRENDTVSALIELGFLSNPK 341 Query: 361 DESIVRTASYQKKAAKAIADGLADYF 386 DESIVR +SYQ+ AAKAIA+GL DYF Sbjct: 342 DESIVRRSSYQRIAAKAIANGLEDYF 367 >C0ZGK0.1 C0ZGK0_BREBN Putative N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 370 Score = 339 bits (870), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 184/388 (47%), Positives = 234/388 (60%), Gaps = 22/388 (5%) Query: 1 MKKTLSTAMLAVTLLLS--NPGHPESAHAAGAYTAKVYASSLNVRSEPAASASVTGTLKS 58 MK T A L L LS P H E A AA AKV A+SLNVRSEPA +ASV T+ Sbjct: 1 MKTTCKQASLLCGLWLSLSWPFHTEPAQAASVIQAKVVATSLNVRSEPAPNASVVATVPQ 60 Query: 59 GALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXXXXXXXXXXXXXXXXXXXXX 118 GA+VT+TDE +GW K+R GWVAGYYL++ Sbjct: 61 GAVVTITDEAYGWAKIRYNQKVGWVAGYYLQKGAVTSAGSASSPANTAVAKSQQGT---- 116 Query: 119 XXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGDWARIRTTGGEVGWVAGQYI 178 V A SLR+R GP T ++++ SL G V +L +QGDW + RT+ G+ GWV+ YI Sbjct: 117 -----VLADSLRMRKGPSTSHEIVLSLPRGTRVDILKKQGDWIQARTSNGQTGWVSATYI 171 Query: 179 ARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPGHGGSDPGMLGTSYDTMEKD 238 ++GK+IVIDPGHGGSD G GT +++MEK Sbjct: 172 GDA-----------KVNANAPVTKSTKSPGLKGKVIVIDPGHGGSDVGTQGTKWNSMEKT 220 Query: 239 LTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQQLRADAFVSIHYNSSPKKV 298 L +TA + LR +GA+V +TRT +KPSL++RV + ADAF+SIHYNSS K Sbjct: 221 LNYKTATLLASKLRQRGAQVFMTRTSDTEKPSLAQRVAFSESKGADAFISIHYNSSVKPN 280 Query: 299 SGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFGDYHILRENSLPATLVELGFLSN 358 SGTLTF+YS+ D LAR+IE+RL GIGL+SNG+SFG+YH+LREN+ P+ L+ELGFLSN Sbjct: 281 SGTLTFYYSQGKDEPLARAIESRLAGGIGLRSNGISFGNYHVLRENNDPSVLIELGFLSN 340 Query: 359 RSDESIVRTASYQKKAAKAIADGLADYF 386 DE IVRT+SYQ KAA+AI + LADYF Sbjct: 341 PKDEGIVRTSSYQDKAAQAITEALADYF 368 >Q8ELX9.1 Q8ELX9_OCEIH N-acetylmuramoyl-L-alanine amidase Length = 379 Score = 201 bits (511), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 132/394 (33%), Positives = 188/394 (47%), Gaps = 24/394 (6%) Query: 1 MKKTLSTAMLAVTLLL---SNPGHPESAHAAGAYTAKVYASSLNVRSEPAASASVTGTLK 57 MK+ S A L +L+ S P HA G T +V +S+LN+RS P+ +S+ G Sbjct: 1 MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFV 60 Query: 58 SGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXXXXXXXXXXXXXXXXXXXX 117 G +T EQ+ W++ G WVA ++L Sbjct: 61 KGNQLTTFKEQYDWVQTYYGGKEVWVAKHHL-------------VPVSTSNVSSTTSEMK 107 Query: 118 XXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGDWARIRTTGGEVGWVAGQY 177 +V V A S+ IR G GT YK S +GD V+ QGDW +++ GE GW+A Sbjct: 108 ENISVTVAAESVMIRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGETGWIASWL 167 Query: 178 IARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKL----IVIDPGHGGSDPGMLGTSYD 233 G L IVIDPGHGG DPG +G D Sbjct: 168 TTEVGGVVSESTASDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLG-D 226 Query: 234 TMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQQLRADAFVSIHYNS 293 EKD+ T+ +V + L+ GA V TR+ G+ SL R +I + DAF+SIHY+S Sbjct: 227 VWEKDIVYSTSNHVVNQLKEAGANVITTRS-GDYFVSLEERARISNDYQTDAFISIHYDS 285 Query: 294 SP-KKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFGDYHILRENSLPATLVE 352 P VSGT T +Y+ D LA I++ + + L + G +Y +LR+ + P+ L+E Sbjct: 286 FPLLSVSGTTT-YYAHKEDQPLAADIQSSIASSVELTNRGSMQANYKVLRDTTAPSVLLE 344 Query: 353 LGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 LGF+SN+ D +IV+T YQ++ A AI +GL YF Sbjct: 345 LGFISNQYDVNIVQTDDYQRRVATAITNGLIQYF 378 >Q71ZE9.1 Q71ZE9_LISMF N-acetylmuramoyl-L-alanine amidase, family 3 EC=3.5.1.28 Length = 427 Score = 195 bits (495), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 123/350 (35%), Positives = 178/350 (50%), Gaps = 36/350 (10%) Query: 40 LNVRSEPAASASVTGTLKSGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXX 99 LNVR +P+ S+ G L +G VTVT +Q+GW +++ S WV+ YL Sbjct: 109 LNVREKPSTSSKALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYL----------- 157 Query: 100 XXXXXXXXXXXXXXXXXXXXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGD 159 TV + S IR P V+ G + QGD Sbjct: 158 ------TIRESVTKVDESELQTVTIRDDSTNIRNKPSRDGAVIEKANSGQGFAIQGVQGD 211 Query: 160 WARIRTTGGEVGWVAGQYIARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPG 219 W +IRTT GE G+VA + + IVIDPG Sbjct: 212 WYKIRTTSGEEGYVANWVV-------------DVSDKGQTSSPRSKTTKLSEATIVIDPG 258 Query: 220 HGGSDPGMLGTSYDTMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQ 279 HGG+DPG G S T+EK++TL+TA ++ L A+GA+V LTR ++ SL R I Sbjct: 259 HGGNDPGAKG-SNGTIEKEMTLKTAKQLKQKLEARGAKVILTR-NSDKYVSLKGRTNIAA 316 Query: 280 QLRADAFVSIHYNS---SPKKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFG 336 + AD F+SIH++S + K VSG T++Y +N+D LA SI L + + + G G Sbjct: 317 ENNADVFISIHFDSLEDASKGVSGQTTYYY-DNSDKSLAESINTTLGKDLPTTNRGARVG 375 Query: 337 DYHILRENSLPATLVELGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 DY+++RENS PA L+ELG+LS+ DES + +ASY+ + A+++ DGL++YF Sbjct: 376 DYYVVRENSQPAVLLELGYLSSAKDESNINSASYRSQIAESVTDGLSNYF 425 >C1KVG8.1 C1KVG8_LISMC Putative N-acetylmuramoyl-L-alanine amidase Length = 427 Score = 195 bits (495), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 123/350 (35%), Positives = 178/350 (50%), Gaps = 36/350 (10%) Query: 40 LNVRSEPAASASVTGTLKSGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXX 99 LNVR +P+ S+ G L +G VTVT +Q+GW +++ S WV+ YL Sbjct: 109 LNVREKPSTSSKALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYL----------- 157 Query: 100 XXXXXXXXXXXXXXXXXXXXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGD 159 TV + S IR P V+ G + QGD Sbjct: 158 ------TIRESVTKVDESELQTVTIRDDSTNIRNKPSRDGAVIEKANSGQGFAIQGVQGD 211 Query: 160 WARIRTTGGEVGWVAGQYIARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPG 219 W +IRTT GE G+VA + + IVIDPG Sbjct: 212 WYKIRTTSGEEGYVANWVV-------------DVSDKGQTSSPRSKTTKLSEATIVIDPG 258 Query: 220 HGGSDPGMLGTSYDTMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQ 279 HGG+DPG G S T+EK++TL+TA ++ L A+GA+V LTR ++ SL R I Sbjct: 259 HGGNDPGAKG-SNGTIEKEMTLKTAKQLKQKLEARGAKVILTR-NSDKYVSLKGRTNIAA 316 Query: 280 QLRADAFVSIHYNS---SPKKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFG 336 + AD F+SIH++S + K VSG T++Y +N+D LA SI L + + + G G Sbjct: 317 ENNADVFISIHFDSLEDASKGVSGQTTYYY-DNSDKSLAESINTTLGKDLPTTNRGARVG 375 Query: 337 DYHILRENSLPATLVELGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 DY+++RENS PA L+ELG+LS+ DES + +ASY+ + A+++ DGL++YF Sbjct: 376 DYYVVRENSQPAVLLELGYLSSAKDESNINSASYRSQIAESVTDGLSNYF 425 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,947,782,112 Number of extensions: 68273503 Number of successful extensions: 218886 Number of sequences better than 1.0e-05: 1652 Number of HSP's gapped: 216556 Number of HSP's successfully gapped: 1681 Length of query: 387 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 249 Effective length of database: 1,760,596,133 Effective search space: 438388437117 Effective search space used: 438388437117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)