BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0013.p (335 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2B035.1 Q2B035_9BACI Putative uncharacterized protein 231 8e-59 A3DDV3.1 A3DDV3_CLOTH Metal dependent phosphohydrolase 220 2e-55 B4BFA5.1 B4BFA5_CLOTM Metal dependent phosphohydrolase 220 2e-55 B2A7I2.1 B2A7I2_NATTJ Metal dependent phosphohydrolase 216 2e-54 C0ZH48.1 C0ZH48_BREBN Putative uncharacterized protein 215 6e-54 >Q2B035.1 Q2B035_9BACI Putative uncharacterized protein Length = 325 Score = 231 bits (589), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 110/268 (41%), Positives = 173/268 (64%) Query: 65 VVNKSKDLFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALFDAIQGKDDYTYQHNIG 124 V + +D+F R+ + V + I+K+++P I+ ++ + +F + KD YT H + Sbjct: 57 AVGQVEDIFAGARLGKGVGITAIKKDILPVIRTLADIPGVSHVFHQLDRKDRYTSSHAVC 116 Query: 125 VGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLTPDEYELVKKHTI 184 V +++ +IGRWL + + + L L + LHDIGK+KIP EIL+KPGKLT EYE +K H++ Sbjct: 117 VSMIAGMIGRWLGIGGALMEDLLLGSILHDIGKIKIPDEILSKPGKLTLSEYEEMKLHSV 176 Query: 185 LGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIVAVADIFHAMSSR 244 GYEM+++ + L LQHHER DGRGYPLGLK +I+ AKI A+AD+FHAMSS Sbjct: 177 YGYEMVRDLDAVSPKAALIVLQHHEREDGRGYPLGLKGREIEHLAKITAIADVFHAMSSA 236 Query: 245 RPYHEPVPFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILTDGRVGEIVYLNP 304 R YHE +PF+ ++ +M+ G+FG+ +P + +F++N++ G V LTD G I+ ++P Sbjct: 237 RIYHEALPFYQVMRKMKDGAFGSFEPGFMLIFIQNLMNSLAGSRVRLTDQSEGRILMIHP 296 Query: 305 HDIETPLVKVDDEYIDLSKLNTIKIKEI 332 +D + LV+ +D IDL ++I+ I Sbjct: 297 YDPLSALVQTEDRLIDLRYSRGLQIERI 324 >A3DDV3.1 A3DDV3_CLOTH Metal dependent phosphohydrolase Length = 345 Score = 220 bits (560), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 122/335 (36%), Positives = 185/335 (55%), Gaps = 12/335 (3%) Query: 6 GKKIKNDLINSYGVTVIPANSILNAEHLELIRKQKINILDIIFAGEEGQAPSCKE---LA 62 G +I ++N YG VI +IL+ ++ + + +I + + E ++ Sbjct: 14 GMRIAETIMNDYGSVVISEGTILDDHMIKKLVNLNYTKVRVIEKDKNFVSSEISESFNVS 73 Query: 63 YDV-VNKSKDLFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALFDAIQGKDDYTYQH 121 Y+ VN K + + + + + + K V+ + + DI + I+ D YTY H Sbjct: 74 YNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQIKSADQYTYTH 133 Query: 122 NIGVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLTPDEYELVKK 181 + V +L L+G+WL S+ L L A LHDIGK K+P EILNKP KLTP+EYE +KK Sbjct: 134 CVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKLTPEEYEEIKK 193 Query: 182 HTILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIVAVADIFHAM 241 HT+ GY +L++ + + + L HHER DG GYP G K +QI+ A+++AVADI+ AM Sbjct: 194 HTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARVIAVADIYDAM 253 Query: 242 SSRRPYHEPVP----FHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILTDGRVG 297 +S R Y E FHII ++ + G LD +V+ FL NI +G L G + Sbjct: 254 TSNRVYREKESPFEVFHIIEEE----AVGKLDVRVVNAFLSNIAAYYIGDFAKLNTGEIC 309 Query: 298 EIVYLNPHDIETPLVKVDDEYIDLSKLNTIKIKEI 332 EIVY+NP + P+++VDD+YIDL+ +KI EI Sbjct: 310 EIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEI 344 >B4BFA5.1 B4BFA5_CLOTM Metal dependent phosphohydrolase Length = 345 Score = 220 bits (560), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 122/335 (36%), Positives = 185/335 (55%), Gaps = 12/335 (3%) Query: 6 GKKIKNDLINSYGVTVIPANSILNAEHLELIRKQKINILDIIFAGEEGQAPSCKE---LA 62 G +I ++N YG VI +IL+ ++ + + +I + + E ++ Sbjct: 14 GMRIAETIMNDYGSVVISEGTILDDHMIKKLVNLNYTKVRVIEKDKNFVSSEISESFNVS 73 Query: 63 YDV-VNKSKDLFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALFDAIQGKDDYTYQH 121 Y+ VN K + + + + + + K V+ + + DI + I+ D YTY H Sbjct: 74 YNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQIKSADQYTYTH 133 Query: 122 NIGVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLTPDEYELVKK 181 + V +L L+G+WL S+ L L A LHDIGK K+P EILNKP KLTP+EYE +KK Sbjct: 134 CVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKLTPEEYEEIKK 193 Query: 182 HTILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIVAVADIFHAM 241 HT+ GY +L++ + + + L HHER DG GYP G K +QI+ A+++AVADI+ AM Sbjct: 194 HTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARVIAVADIYDAM 253 Query: 242 SSRRPYHEPVP----FHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILTDGRVG 297 +S R Y E FHII ++ + G LD +V+ FL NI +G L G + Sbjct: 254 TSNRVYREKESPFEVFHIIEEE----AVGKLDVRVVNAFLSNIAAYYIGDFAKLNTGEIC 309 Query: 298 EIVYLNPHDIETPLVKVDDEYIDLSKLNTIKIKEI 332 EIVY+NP + P+++VDD+YIDL+ +KI EI Sbjct: 310 EIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEI 344 >B2A7I2.1 B2A7I2_NATTJ Metal dependent phosphohydrolase Length = 346 Score = 216 bits (551), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 124/336 (36%), Positives = 188/336 (55%), Gaps = 15/336 (4%) Query: 1 MERYL------GKKIKNDLINSYGVTVIPANSILNAEHLELIRKQKINILDI------IF 48 M+RY G K+ D+ + G T++P IL+ + ++ + I + I I Sbjct: 1 MKRYSVFQLKEGMKLSKDVNSKSGATLVPKGVILSKKTIKFLINWGIKSVLIEEEEEQIT 60 Query: 49 AGEEGQAPSCKELAYDVVNKSKDLFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALF 108 G K+ ++ K E + S+ + L ++ KE+ + S + L Sbjct: 61 RGRGHSQAKFKKEYFETTEKVISFMEGLKGSKAIELDEV-KEIAQELVTYSDDINSVKLL 119 Query: 109 DAIQGKDDYTYQHNIGVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKP 168 ++ KD YTYQH+I VG+ ++L+GRWL +D+ LSLA LHDIGK +IP E+L KP Sbjct: 120 TEVKDKDYYTYQHSINVGIYASLLGRWLGYCTNDIKNLSLAGLLHDIGKTQIPDEVLLKP 179 Query: 169 GKLTPDEYELVKKHTILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAY 228 LT E+E++K H I GYE+LK + I + LQHHER +G GYP G+ E+I +Y Sbjct: 180 ESLTDKEFEIIKNHPIYGYELLKPCEDLSPEINIAVLQHHERENGSGYPFGITGEKIHSY 239 Query: 229 AKIVAVADIFHAMSSRRPYH-EPVPFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGR 287 +KI++V DIF A++S R Y PF I ++R SF L+P I F + I + VG Sbjct: 240 SKIISVVDIFDAITSNRVYKGRNSPFEAI-REVRDKSFQDLNPEIAYTFFKRISEFFVGS 298 Query: 288 EVILTDGRVGEIVYLNPHDIETPLVKVDDEYIDLSK 323 VI DG +G+IV+LN +D PLV+++D+++DLSK Sbjct: 299 TVIFNDGSIGKIVFLNKNDPTRPLVQIEDKFVDLSK 334 >C0ZH48.1 C0ZH48_BREBN Putative uncharacterized protein Length = 360 Score = 215 bits (547), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 108/277 (38%), Positives = 169/277 (61%), Gaps = 1/277 (0%) Query: 54 QAPSCKELAYD-VVNKSKDLFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALFDAIQ 112 QA + ++D V+ K+L + +P+ ++R+ V P I E + + Sbjct: 78 QAKPVFQESFDKAVSTLKNLMTRVQGGNNIPVMEVREVVTPIITEFQQQPQVLLSLRRFA 137 Query: 113 GKDDYTYQHNIGVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLT 172 D Y Y+H + VG++S +I +W+ + E + ++LA TL DIGK KI IL KPGKLT Sbjct: 138 KMDSYAYEHAVAVGIISYMIAKWVKVPEKEWMQVALAGTLLDIGKTKIDRRILQKPGKLT 197 Query: 173 PDEYELVKKHTILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIV 232 PDE+E +KKHT+ GY+++K + G + + L ALQHHER DG GYPLGL ++ Y+KIV Sbjct: 198 PDEFEEMKKHTVYGYQIIKSSHGLSEGVALAALQHHEREDGSGYPLGLPGSKLHLYSKIV 257 Query: 233 AVADIFHAMSSRRPYHEPVPFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILT 292 AVAD++HAMSS R + + + + +V+Q+ + SFG LDP IV F+E I + VG V L+ Sbjct: 258 AVADVYHAMSSDRVHQKALSPYQVVEQLVQDSFGKLDPTIVRSFVEGITQFAVGTLVELS 317 Query: 293 DGRVGEIVYLNPHDIETPLVKVDDEYIDLSKLNTIKI 329 DG +G+IV+ + + P+V+ + ++L + + I Sbjct: 318 DGTIGKIVFTDRNHPTRPMVETGGKIVNLVEARHLSI 354 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,979,077,693 Number of extensions: 82931695 Number of successful extensions: 219979 Number of sequences better than 1.0e-05: 2518 Number of HSP's gapped: 217930 Number of HSP's successfully gapped: 2522 Length of query: 335 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 199 Effective length of database: 1,779,438,163 Effective search space: 354108194437 Effective search space used: 354108194437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)