BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0007.p (431 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1PFB4.1 C1PFB4_BACCO Mn2+/Fe2+ transporter, NRAMP family 561 e-158 C2ZN57.1 C2ZN57_BACCE Manganese transport protein mntH 551 e-155 C2Z6H0.1 C2Z6H0_BACCE Manganese transport protein mntH 551 e-155 A9VQI5.1 A9VQI5_BACWK Mn2+/Fe2+ transporter, NRAMP family 551 e-155 C3A4I8.1 C3A4I8_BACMY Manganese transport protein mntH 551 e-155 >C1PFB4.1 C1PFB4_BACCO Mn2+/Fe2+ transporter, NRAMP family Length = 430 Score = 561 bits (1447), Expect = e-158, Method: Compositional matrix adjust. Identities = 283/431 (65%), Positives = 334/431 (77%), Gaps = 1/431 (0%) Query: 1 MMEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNI 60 M E+ +++ P N +AQAVL GDVKGLKR+LPFLGPAFIA VAY+DPGNFATNI Sbjct: 1 MSEKTMSQKMPG-RTSHNTTSAQAVLRGDVKGLKRILPFLGPAFIAGVAYIDPGNFATNI 59 Query: 61 TAGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSE 120 TAGSKYGY+LLWVI SNLMAVLIQSLSAKLGIATGKNLPEVA + FPK VSIFLWIQSE Sbjct: 60 TAGSKYGYMLLWVIAFSNLMAVLIQSLSAKLGIATGKNLPEVAHDHFPKPVSIFLWIQSE 119 Query: 121 LVIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVL 180 LVIIATDLAEFIGAALGLYL+FG+P+LPAALITAVGSFAILELQRRGYR+ EA IA +V+ Sbjct: 120 LVIIATDLAEFIGAALGLYLIFGLPLLPAALITAVGSFAILELQRRGYRSFEAVIAALVM 179 Query: 181 IVVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQS 240 IVVLAF Q +AKP+ V GI TP+F G+ SI+LAAGILGATVMPHAIYLHSSLTQ+ Sbjct: 180 IVVLAFALQTFLAKPEWVKVFGGIVTPKFDGIQSIVLAAGILGATVMPHAIYLHSSLTQN 239 Query: 241 RVVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQF 300 R+VG ++ EK++IFK E IDI+IAM+I +F+KNGLVVEDLD+AF +F Sbjct: 240 RIVGKNELEKRKIFKFELIDIIIAMVIAGAINMSMLVIASSVFYKNGLVVEDLDVAFREF 299 Query: 301 RHLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXX 360 GP A+SF T++GDVVMQGFINKRINLYLRR Sbjct: 300 YTHIGPAAAISFGLGLLIAGLSSSSVGTLSGDVVMQGFINKRINLYLRRAITMIPPLLII 359 Query: 361 XFGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVS 420 G N TSALV+SQV+LSFGI FAL+PL++FTS+R+IM LVNHRIT+++ WI++ VV Sbjct: 360 MMGTNPTSALVISQVILSFGIIFALVPLILFTSNRQIMGELVNHRITSIISWIVAGFVVC 419 Query: 421 LNLFLIVEMFV 431 LNLFL+++ F+ Sbjct: 420 LNLFLLIDTFI 430 >C2ZN57.1 C2ZN57_BACCE Manganese transport protein mntH Length = 431 Score = 551 bits (1420), Expect = e-155, Method: Compositional matrix adjust. Identities = 281/430 (65%), Positives = 326/430 (75%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++ + + + +A L+G KG KRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDITKDEQVPSQSTTVQSAHLALSGQTKGFKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG+NLPEVARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGRNLPEVARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITAVGSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+FTP+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGIQVFYAKPELSPLLSGLFTPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG +D++KK+IF+ EFIDI+IAM+I LFFKNGL VEDLD+AF QF Sbjct: 241 VVGTNDEQKKKIFRFEFIDIIIAMVIAGAINASMLIVAAALFFKNGLHVEDLDVAFNQFS 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 TLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS +KIM LVNHRITT + WII+ALV+ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSSKKIMGALVNHRITTFIAWIIAALVIIL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 >C2Z6H0.1 C2Z6H0_BACCE Manganese transport protein mntH Length = 431 Score = 551 bits (1420), Expect = e-155, Method: Compositional matrix adjust. Identities = 281/430 (65%), Positives = 326/430 (75%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++ + + + +A L+G KG KRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDITKDEQVPSQSTTVQSAHLALSGQTKGFKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG+NLPEVARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGRNLPEVARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITAVGSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+FTP+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGIQVFYAKPELSPLLSGLFTPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG +D++KK+IF+ EFIDI+IAM+I LFFKNGL VEDLD+AF QF Sbjct: 241 VVGTNDEQKKKIFRFEFIDIIIAMVIAGAINASMLIVAAALFFKNGLHVEDLDVAFNQFS 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 TLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS +KIM LVNHRITT + WII+ALV+ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSSKKIMGALVNHRITTFIAWIIAALVIIL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 >A9VQI5.1 A9VQI5_BACWK Mn2+/Fe2+ transporter, NRAMP family Length = 431 Score = 551 bits (1419), Expect = e-155, Method: Compositional matrix adjust. Identities = 279/430 (64%), Positives = 328/430 (76%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++ + + + +A L+G KG KRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDITKDEQVPSQSTTVQSAHLALSGQTKGFKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG+NLPEVARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGRNLPEVARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITAVGSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+FTP+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGIQVFYAKPELSPLLSGLFTPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG +D++KK+IF+ EFIDI+IAM+I LFFKNGL VEDLD+AF QF Sbjct: 241 VVGTNDEQKKKIFRFEFIDIIIAMVIAGAINASMLIVAAALFFKNGLHVEDLDVAFNQFS 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 +L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 NLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS++KIM LVNHRITT + W+I+AL++ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSNKKIMGALVNHRITTFIAWLIAALIIVL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 >C3A4I8.1 C3A4I8_BACMY Manganese transport protein mntH Length = 431 Score = 551 bits (1419), Expect = e-155, Method: Compositional matrix adjust. Identities = 279/430 (64%), Positives = 328/430 (76%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++ + + + +A L+G KG KRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDITKDEQVPSQSTTVQSAHLALSGQTKGFKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG+NLPEVARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGRNLPEVARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITAVGSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+FTP+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGIQVFYAKPELSPLLSGLFTPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG +D++KK+IF+ EFIDI+IAM+I LFFKNGL VEDLD+AF QF Sbjct: 241 VVGTNDEQKKKIFRFEFIDIIIAMVIAGAINASMLIVAAALFFKNGLHVEDLDVAFNQFS 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 +L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 NLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS++KIM LVNHRITT + W+I+AL++ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSNKKIMGALVNHRITTFIAWLIAALIIVL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,036,169,392 Number of extensions: 72950671 Number of successful extensions: 281580 Number of sequences better than 1.0e-05: 1435 Number of HSP's gapped: 279127 Number of HSP's successfully gapped: 1452 Length of query: 431 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 292 Effective length of database: 1,751,175,118 Effective search space: 511343134456 Effective search space used: 511343134456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)