BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0021.p (250 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4BZU2.1 C4BZU2_9FUSO DNA-3-methyladenine glycosylase 175 3e-42 C3DFV3.1 C3DFV3_BACTS 3-methyladenine DNA glycosylase 174 1e-41 B7IJ32.1 3MGH_BACC2 Putative 3-methyladenine DNA glycosylase EC=... 173 1e-41 C3GEU2.1 C3GEU2_BACTU 3-methyladenine DNA glycosylase 173 2e-41 C2PAY6.1 C2PAY6_BACCE 3-methyladenine DNA glycosylase 172 2e-41 >C4BZU2.1 C4BZU2_9FUSO DNA-3-methyladenine glycosylase Length = 197 Score = 175 bits (443), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 87/188 (46%), Positives = 121/188 (64%), Gaps = 4/188 (2%) Query: 51 ALQAGPLLLGQHLVRRTEDGEIRCRIVETESYGGAEDKGSHAYGGRRTARTEVMFSAGGT 110 AL+ GP +LG +L+R+ D ++ I E E+Y G EDKG+H Y +RTARTE MFS GG Sbjct: 12 ALEVGPEILGHYLIRKINDRTVKTMITEVEAYVGPEDKGAHTYKNKRTARTEPMFSEGGH 71 Query: 111 AYVYLIYGMYHCVNVVTAAEHDPHAVLIRAVEPLTSTDAARMAAYRGITVKKPSSLSGGP 170 AYVYLIYGMY+C+N+V + P A+L+RAVEPL D + VK +LS GP Sbjct: 72 AYVYLIYGMYNCINIVCQRKGKPEALLLRAVEPLNEFD---LLFDNRSPVKNIHNLSNGP 128 Query: 171 GKLCRALRIDKSLNGCRLDDPLGPLWLEQGDSPEHLDIAAAPRINIPYAEEYAPLPWRFY 230 GKLC AL ID++ +G L L+LE+ ++ + +++ + RI I YAEEY WRFY Sbjct: 129 GKLCSALGIDRTFSGYDLISG-KELYLEKNENRKDIEVVCSKRIGIDYAEEYKDKLWRFY 187 Query: 231 LRGNPYVS 238 ++ N ++S Sbjct: 188 IKNNKFIS 195 >C3DFV3.1 C3DFV3_BACTS 3-methyladenine DNA glycosylase Length = 205 Score = 174 bits (440), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 98/201 (48%), Positives = 120/201 (59%), Gaps = 8/201 (3%) Query: 44 PDLYKLTALQAGPLLLGQHLVRRTEDGEIRCRIVETESYGGAEDKGSHAYGGRRTARTEV 103 P Y+ L+ LLGQ LV + + IVE E+Y G +DK +H+YGGRRT RTEV Sbjct: 5 PSFYEGDTLEVAKKLLGQKLVHIVDGIKRSGIIVEVEAYKGPDDKAAHSYGGRRTDRTEV 64 Query: 104 MFSAGGTAYVYLIYGMYHCVNVVTAAEHDPHAVLIRAVEPLTSTDAARMAAYRG--ITVK 161 MF A G AYVYLIYGMYHC NV+TA P VLIRA+EP+ + ++A Y IT Sbjct: 65 MFGAPGHAYVYLIYGMYHCFNVITAPAGTPQGVLIRALEPVDGIEEIKLARYNKTDITKA 124 Query: 162 KPSSLSGGPGKLCRALRIDKSLNGCRLDDPLGPLWLEQGDSPEHLD----IAAAPRINIP 217 + +L+ GPGKLCRAL I G L L +E EH+ IAA PRINI Sbjct: 125 QYKNLTSGPGKLCRALGITLKERGLSLQS--DTLHIELVPKDEHISSQYKIAAGPRINID 182 Query: 218 YAEEYAPLPWRFYLRGNPYVS 238 YAEE PWRFY +G+P+VS Sbjct: 183 YAEEAVHYPWRFYYKGHPFVS 203 >B7IJ32.1 3MGH_BACC2 Putative 3-methyladenine DNA glycosylase EC=3.2.2.- Length = 205 Score = 173 bits (439), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 98/201 (48%), Positives = 120/201 (59%), Gaps = 8/201 (3%) Query: 44 PDLYKLTALQAGPLLLGQHLVRRTEDGEIRCRIVETESYGGAEDKGSHAYGGRRTARTEV 103 P Y+ L+ LLGQ LV + + IVE E+Y G +DK +H+YGGRRT RTEV Sbjct: 5 PSFYEGDTLEVAKKLLGQKLVHIVDGIKRSGIIVEVEAYKGPDDKAAHSYGGRRTDRTEV 64 Query: 104 MFSAGGTAYVYLIYGMYHCVNVVTAAEHDPHAVLIRAVEPLTSTDAARMAAYRG--ITVK 161 MF A G AYVYLIYGMYHC NV+TA P VLIRA+EP+ + ++A Y IT Sbjct: 65 MFGAPGHAYVYLIYGMYHCFNVITAPVGTPQGVLIRALEPVDGIEEIKLARYNKTDITKA 124 Query: 162 KPSSLSGGPGKLCRALRIDKSLNGCRLDDPLGPLWLEQGDSPEHLD----IAAAPRINIP 217 + +L+ GPGKLCRAL I G L L +E EH+ IAA PRINI Sbjct: 125 QYKNLTSGPGKLCRALGITLKERGLSLQS--DTLHIELVPKDEHISSQYKIAAGPRINID 182 Query: 218 YAEEYAPLPWRFYLRGNPYVS 238 YAEE PWRFY +G+P+VS Sbjct: 183 YAEEAVHYPWRFYYKGHPFVS 203 >C3GEU2.1 C3GEU2_BACTU 3-methyladenine DNA glycosylase Length = 205 Score = 173 bits (438), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 98/201 (48%), Positives = 119/201 (59%), Gaps = 8/201 (3%) Query: 44 PDLYKLTALQAGPLLLGQHLVRRTEDGEIRCRIVETESYGGAEDKGSHAYGGRRTARTEV 103 P Y+ L+ LLGQ LV + + IVE E+Y G +DK +H+YGGRRT RTEV Sbjct: 5 PSFYEGDTLEVAKKLLGQKLVHIVDGVKRIGIIVEVEAYKGPDDKAAHSYGGRRTDRTEV 64 Query: 104 MFSAGGTAYVYLIYGMYHCVNVVTAAEHDPHAVLIRAVEPLTSTDAARMAAYRG--ITVK 161 MFSA G AYVYLIYGMYHC NV+TA P VLIRA+EP+ + ++A Y IT Sbjct: 65 MFSAPGHAYVYLIYGMYHCFNVITAPVGTPQGVLIRALEPVDGIEEIKLARYNKTDITKA 124 Query: 162 KPSSLSGGPGKLCRALRIDKSLNGCRLDDPLGPLWLEQGDSPEHLD----IAAAPRINIP 217 + +L+ GPGKLCRAL I G L L +E EH+ I A PRINI Sbjct: 125 QYKNLTNGPGKLCRALGITLEERGVSLQS--DTLHIELVPKEEHISSQYKITAGPRINID 182 Query: 218 YAEEYAPLPWRFYLRGNPYVS 238 YAEE PWRFY G+P+VS Sbjct: 183 YAEEAVHYPWRFYYEGHPFVS 203 >C2PAY6.1 C2PAY6_BACCE 3-methyladenine DNA glycosylase Length = 205 Score = 172 bits (436), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 97/201 (48%), Positives = 118/201 (58%), Gaps = 8/201 (3%) Query: 44 PDLYKLTALQAGPLLLGQHLVRRTEDGEIRCRIVETESYGGAEDKGSHAYGGRRTARTEV 103 P Y+ L+ LLGQ LV + + IVE E+Y G +DK +H+YGGRRT RTEV Sbjct: 5 PSFYEGDTLEVAKKLLGQKLVHTVDGIKRSGIIVEVEAYKGPDDKAAHSYGGRRTDRTEV 64 Query: 104 MFSAGGTAYVYLIYGMYHCVNVVTAAEHDPHAVLIRAVEPLTSTDAARMAAYRG--ITVK 161 MF A G AYVYLIYGMYHC NV+TA P VLIRA+EP+ + ++A Y IT Sbjct: 65 MFGAPGHAYVYLIYGMYHCFNVITAPVGTPQGVLIRALEPVDGIEEIKLARYNKTDITKA 124 Query: 162 KPSSLSGGPGKLCRALRIDKSLNGCRLDDPLGPLWLEQGDSPEHLD----IAAAPRINIP 217 + +L+ GPGKLCRAL I G L L +E EH+ I A PRINI Sbjct: 125 QYKNLTNGPGKLCRALGITLEERGVSLQS--DTLHIELVPEEEHISSQYKITAGPRINID 182 Query: 218 YAEEYAPLPWRFYLRGNPYVS 238 YAEE PWRFY G+P+VS Sbjct: 183 YAEEAVQYPWRFYYEGHPFVS 203 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,620,075,940 Number of extensions: 68588680 Number of successful extensions: 116841 Number of sequences better than 1.0e-05: 615 Number of HSP's gapped: 115479 Number of HSP's successfully gapped: 615 Length of query: 250 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 118 Effective length of database: 1,817,122,223 Effective search space: 214420422314 Effective search space used: 214420422314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)