BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0007.p (237 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK95.1 B3KK95_9BACI Pseudouridine synthase EC=5.4.99.- 386 e-106 B1DKA5.1 B1DKA5_9BACL Pseudouridine synthase EC=5.4.99.- 381 e-104 C0Z4U9.1 C0Z4U9_BREBN Pseudouridine synthase EC=4.2.1.70 338 3e-91 A6CL09.1 A6CL09_9BACI Pseudouridine synthase EC=5.4.99.- 326 1e-87 A8MHI9.1 A8MHI9_ALKOO Pseudouridine synthase EC=5.4.99.- 317 4e-85 >B3KK95.1 B3KK95_9BACI Pseudouridine synthase EC=5.4.99.- Length = 233 Score = 386 bits (991), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/233 (81%), Positives = 209/233 (89%) Query: 5 VRINKFISETGFCSRREADKLVEGGRVTINGERAVLGSQAEPGDDVRIDGKRLETGSKVV 64 +RINKFISETG+CSRREADKLVE G+VTING AVLGSQAE GDDVRI GK L+ K V Sbjct: 1 MRINKFISETGYCSRREADKLVESGKVTINGVVAVLGSQAEEGDDVRIQGKPLQDKKKHV 60 Query: 65 YIALNKPVGITSTTEAHIQGNIVDFVGHHERIFPIGRLDKDSEGLILLTNDGDIVNKILR 124 YIALNKPVGITSTTE+HI+GNIVDFVGH+ERIFPIGRLDKDSEGLILLTNDGDIVNKILR Sbjct: 61 YIALNKPVGITSTTESHIKGNIVDFVGHNERIFPIGRLDKDSEGLILLTNDGDIVNKILR 120 Query: 125 AEGKHEKEYIVTVDRPVTPSFVTGMSSGVKILGSRTLPCQVTRISERVFRIILTEGKNRQ 184 +EGKHEKEYIVTVDRPVT SF+ GM+ GVKILG TLPC+VTR+SERVFRIILTEGKNRQ Sbjct: 121 SEGKHEKEYIVTVDRPVTESFLRGMAGGVKILGEMTLPCEVTRMSERVFRIILTEGKNRQ 180 Query: 185 IRRMCAAFGYEVRRLQRIRVMNIHLGSLQTGEWRELTAPEKMELGAMLNYQLQ 237 IRRMC+AFGYEVR+LQRIR+MNI LGS + G WR+L EK ELG +LNY+L+ Sbjct: 181 IRRMCSAFGYEVRKLQRIRIMNIRLGSQRIGSWRDLNDKEKEELGRLLNYKLE 233 >B1DKA5.1 B1DKA5_9BACL Pseudouridine synthase EC=5.4.99.- Length = 233 Score = 381 bits (978), Expect = e-104, Method: Compositional matrix adjust. Identities = 186/231 (80%), Positives = 204/231 (88%) Query: 5 VRINKFISETGFCSRREADKLVEGGRVTINGERAVLGSQAEPGDDVRIDGKRLETGSKVV 64 +RINKFISETG+CSRREADKLV+ GRVTING +A LGSQAE GDDVRIDGKR+ V Sbjct: 1 MRINKFISETGYCSRREADKLVDSGRVTINGAKAELGSQAELGDDVRIDGKRIGEQKHHV 60 Query: 65 YIALNKPVGITSTTEAHIQGNIVDFVGHHERIFPIGRLDKDSEGLILLTNDGDIVNKILR 124 YIALNKPVGITSTTE HI+GNIVDFVGH ERIFPIGRLDKDSEGLILLTNDGD+VN ILR Sbjct: 61 YIALNKPVGITSTTELHIRGNIVDFVGHKERIFPIGRLDKDSEGLILLTNDGDVVNPILR 120 Query: 125 AEGKHEKEYIVTVDRPVTPSFVTGMSSGVKILGSRTLPCQVTRISERVFRIILTEGKNRQ 184 +EGKHEKEYIVTVDRPVT FV GMSSGV+ILGS TLPCQVTR++ERVFRI+LTEGKNRQ Sbjct: 121 SEGKHEKEYIVTVDRPVTAEFVKGMSSGVRILGSMTLPCQVTRVAERVFRIVLTEGKNRQ 180 Query: 185 IRRMCAAFGYEVRRLQRIRVMNIHLGSLQTGEWRELTAPEKMELGAMLNYQ 235 IRRMC AFGY VR+LQRIR+MNIHLG L TG+WR+LT EK EL L+Y+ Sbjct: 181 IRRMCEAFGYHVRKLQRIRIMNIHLGQLPTGKWRDLTETEKAELFGSLDYR 231 >C0Z4U9.1 C0Z4U9_BREBN Pseudouridine synthase EC=4.2.1.70 Length = 228 Score = 338 bits (866), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 160/228 (70%), Positives = 191/228 (83%) Query: 5 VRINKFISETGFCSRREADKLVEGGRVTINGERAVLGSQAEPGDDVRIDGKRLETGSKVV 64 +RINKFISETG CSRREADKL+E RVTING+ A LGS GDDVRIDG+ L K V Sbjct: 1 MRINKFISETGICSRREADKLIESKRVTINGQLAELGSTVASGDDVRIDGQPLGVKKKDV 60 Query: 65 YIALNKPVGITSTTEAHIQGNIVDFVGHHERIFPIGRLDKDSEGLILLTNDGDIVNKILR 124 YIALNKPVGIT TTE H++GNI+DFV H ERIFPIGRLDKDS+GLILLTNDGDIVNKILR Sbjct: 61 YIALNKPVGITCTTELHVKGNIIDFVNHPERIFPIGRLDKDSQGLILLTNDGDIVNKILR 120 Query: 125 AEGKHEKEYIVTVDRPVTPSFVTGMSSGVKILGSRTLPCQVTRISERVFRIILTEGKNRQ 184 AE H+KEYIVTVD+P+T +F+ GM++GV+ILG+ T PC+VT++S+RVF I+LT+G NRQ Sbjct: 121 AENNHDKEYIVTVDKPITANFLHGMANGVRILGTTTKPCKVTKVSDRVFNIVLTQGLNRQ 180 Query: 185 IRRMCAAFGYEVRRLQRIRVMNIHLGSLQTGEWRELTAPEKMELGAML 232 IRRMC AFGY+VR+L+RIR+MNI LG+L+ G+WR LTA E +L L Sbjct: 181 IRRMCQAFGYQVRKLERIRIMNIALGNLKLGQWRNLTAKELTDLNKNL 228 >A6CL09.1 A6CL09_9BACI Pseudouridine synthase EC=5.4.99.- Length = 238 Score = 326 bits (835), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 153/233 (65%), Positives = 192/233 (82%) Query: 3 IEVRINKFISETGFCSRREADKLVEGGRVTINGERAVLGSQAEPGDDVRIDGKRLETGSK 62 E+RINKFISE G SRR AD+L+E GRVTING++A +G Q EPGDD+R+ G+++ Sbjct: 2 FELRINKFISEAGKASRRGADRLIEEGRVTINGKKAKIGDQVEPGDDIRVSGQQIRVARN 61 Query: 63 VVYIALNKPVGITSTTEAHIQGNIVDFVGHHERIFPIGRLDKDSEGLILLTNDGDIVNKI 122 VY+ALNKPVGITSTTE +++GNIVD V H R+F IGRLDKDS+GLILLTNDGDIVN+I Sbjct: 62 NVYLALNKPVGITSTTEKNVKGNIVDLVNHPLRVFNIGRLDKDSDGLILLTNDGDIVNEI 121 Query: 123 LRAEGKHEKEYIVTVDRPVTPSFVTGMSSGVKILGSRTLPCQVTRISERVFRIILTEGKN 182 LRAE +HEKEYIV+VD+P+TP F+T MS GVKILG++TLPC+V ++S+ F+IILT+G N Sbjct: 122 LRAENQHEKEYIVSVDKPITPEFLTQMSEGVKILGTKTLPCKVEQLSKFDFKIILTQGLN 181 Query: 183 RQIRRMCAAFGYEVRRLQRIRVMNIHLGSLQTGEWRELTAPEKMELGAMLNYQ 235 RQIRRMC A GYEV RLQR+R+MNIHLG+L G+WR+LT EK +L L+Y+ Sbjct: 182 RQIRRMCEALGYEVFRLQRVRIMNIHLGNLPVGQWRDLTKKEKQQLFRELDYE 234 >A8MHI9.1 A8MHI9_ALKOO Pseudouridine synthase EC=5.4.99.- Length = 228 Score = 317 bits (813), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 153/220 (69%), Positives = 180/220 (81%) Query: 5 VRINKFISETGFCSRREADKLVEGGRVTINGERAVLGSQAEPGDDVRIDGKRLETGSKVV 64 +RINK+ISETG CSRREAD+ + RVTING A LGS PGD V +D K + K V Sbjct: 1 MRINKYISETGICSRREADQWIIDKRVTINGIVAELGSDVSPGDAVFVDHKPIGQKRKPV 60 Query: 65 YIALNKPVGITSTTEAHIQGNIVDFVGHHERIFPIGRLDKDSEGLILLTNDGDIVNKILR 124 YIALNKPVGIT TTE H++GNIVDFV H ERIFPIGRLDKDS+GLILLTNDGDIVNKILR Sbjct: 61 YIALNKPVGITCTTEKHVKGNIVDFVHHKERIFPIGRLDKDSQGLILLTNDGDIVNKILR 120 Query: 125 AEGKHEKEYIVTVDRPVTPSFVTGMSSGVKILGSRTLPCQVTRISERVFRIILTEGKNRQ 184 AE HEKEYIVTV++P+TP F+ GMSSGVKILG++T PC+VT+I++ VFRIILT+G NRQ Sbjct: 121 AENNHEKEYIVTVNKPITPEFIKGMSSGVKILGTKTKPCKVTKINDTVFRIILTQGLNRQ 180 Query: 185 IRRMCAAFGYEVRRLQRIRVMNIHLGSLQTGEWRELTAPE 224 IRRMC FGY+V +LQRIR+MN+ L L+ G+WR+LT E Sbjct: 181 IRRMCQVFGYKVVKLQRIRIMNVKLKGLEIGQWRDLTKEE 220 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,372,923,086 Number of extensions: 57013509 Number of successful extensions: 150742 Number of sequences better than 1.0e-05: 3518 Number of HSP's gapped: 143835 Number of HSP's successfully gapped: 3525 Length of query: 237 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 105 Effective length of database: 1,817,122,223 Effective search space: 190797833415 Effective search space used: 190797833415 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)