BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0003.p (485 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8Y6.1 B3K8Y6_9BACI Polysaccharide biosynthesis protein 412 e-113 C0ZHE2.1 C0ZHE2_BREBN Conserved hypothetical membrane protein 231 2e-58 C0Z9G8.1 C0Z9G8_BREBN Putative polysaccharide biosynthesis protein 188 1e-45 C2U7X2.1 C2U7X2_BACCE Polysaccharide synthase 186 5e-45 Q81J92.1 Q81J92_BACCR Polysaccharides export protein 184 2e-44 >B3K8Y6.1 B3K8Y6_9BACI Polysaccharide biosynthesis protein Length = 559 Score = 412 bits (1060), Expect = e-113, Method: Compositional matrix adjust. Identities = 224/409 (54%), Positives = 284/409 (69%), Gaps = 2/409 (0%) Query: 22 MKLKSQGSKLLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTV 81 MK G++LLQGAFILSAAAILSKLIGTLQKIPLQN+GGDAVFGIYNTVYP Y M++T+ Sbjct: 1 MKPDQTGARLLQGAFILSAAAILSKLIGTLQKIPLQNMGGDAVFGIYNTVYPFYMMIITI 60 Query: 82 AMLGLPSAVSKFVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGN 141 A +G P+AVSK+VAE A ++G R+L+LS+ G+++G L+YA AP I W+G+ Sbjct: 61 AAIGFPAAVSKYVAEYEAEGRTRDGHRLLRLSSAALVLFGLILGFLMYACAPWIGKWIGS 120 Query: 142 SHVTPALRSSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTS 201 S V PALR+ A LA VP M+ LRGYFQGLHNMVPTA+SQ+ EQ VRV VMI LLLYL Sbjct: 121 SQVIPALRAGALALAFVPWMSVLRGYFQGLHNMVPTAISQITEQMVRVGVMIVLLLYLIR 180 Query: 202 IHADXXXXXXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEP-ELAAASETAGGTSWTKEQR 260 + AD +M +Y R+H R + AA +G + K + Sbjct: 181 VGADADVIAAGALLGSAAGGAAGLLVMGIYWRKHIRASRSTSWGAARHESGESGMNKTEM 240 Query: 261 QLALENRRERIRIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGG-SEAAAMAQFG 319 + + + +G+LLAYG+PV L LAVPLIGLVD FTVPRLL+SGG E+ AMA+FG Sbjct: 241 RADVTKEGAGVLLGRLLAYGIPVCLSLLAVPLIGLVDTFTVPRLLASGGLGESLAMAEFG 300 Query: 320 IYNRGLPLVQIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXX 379 +YNRGLPLVQ+VTMLA SLSV+FIPALAEAK++G+ + ++ ++LRWFW Sbjct: 301 VYNRGLPLVQLVTMLAASLSVLFIPALAEAKFKGESDRVASQSRMALRWFWLIGMTASVG 360 Query: 380 XXXXXEPVNIALYGDASGSSTMVWLAFTAAGGTVSIISAALLQGLGAVR 428 EP+NI LY DA+G+ TM +AFTAAGGT+S I+AALLQG+G V+ Sbjct: 361 LAVLAEPINIMLYEDAAGTGTMQVIAFTAAGGTLSTITAALLQGIGMVK 409 >C0ZHE2.1 C0ZHE2_BREBN Conserved hypothetical membrane protein Length = 554 Score = 231 bits (588), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 140/399 (35%), Positives = 215/399 (53%), Gaps = 14/399 (3%) Query: 30 KLLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPSA 89 + ++GA IL A ++SKL+G + +IP QN+ GD +Y VYPLYT L+ +A G P A Sbjct: 9 QFVKGAAILGIAGLVSKLLGAVYRIPYQNIAGDIGLYVYMQVYPLYTALLILATAGFPIA 68 Query: 90 VSKFVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGNSHVTPALR 149 +SK V+E A RR +++++ G+ +L+Y GAP+I+ +G+ H+ LR Sbjct: 69 ISKIVSERVAVGDAIGARRAFRVASISLVVLGIFFFLLLYGGAPIIASMMGDEHLITPLR 128 Query: 150 SSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTSIHADXXXX 209 + AW L +VP+ A LRGYFQG NM+PT VSQVAEQ +RV ++ + +++ D Sbjct: 129 AVAWSLPLVPMAAILRGYFQGHQNMMPTGVSQVAEQFIRVIFILVAAFWAMNVYQDPYLA 188 Query: 210 XXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLALENRRE 269 ++L Y R ++ + + +++ WT R+ Sbjct: 189 GTGAVFAAFPGAIAAILVLLWYWRADKKMGQLSMGQVQQSS-VQPWTN----------RQ 237 Query: 270 RIRIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLS-SGGSEAAAMAQFGIYNRGLPLV 328 +R LL Y +P+ +GAL +PL+ LVD TV +L SG E A G ++RG PL+ Sbjct: 238 ILR--HLLTYAVPICMGALVLPLVPLVDSMTVVNILQWSGTPEDLAKLLKGAFDRGQPLI 295 Query: 329 QIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXXXXXEPVN 388 Q T ATSLS+ +PA++EA + LI R +++R + EP+N Sbjct: 296 QFGTFFATSLSLALVPAISEAVAQRQHQLISHRSEVAIRLTFLLGLPASFGLALLAEPIN 355 Query: 389 IALYGDASGSSTMVWLAFTAAGGTVSIISAALLQGLGAV 427 + LYGD++G+ + +FT TVSI SA +LQGLG V Sbjct: 356 VMLYGDSNGTEALAVQSFTIIFATVSIASAGILQGLGRV 394 >C0Z9G8.1 C0Z9G8_BREBN Putative polysaccharide biosynthesis protein Length = 550 Score = 188 bits (477), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 126/401 (31%), Positives = 198/401 (49%), Gaps = 16/401 (3%) Query: 27 QGSKLLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGL 86 + S L GA IL+ A ILSK+IG +IPLQ + GD G+Y VYPLY + +A G+ Sbjct: 3 KKSHFLAGALILAIAGILSKVIGMFYRIPLQEIVGDRGLGLYQEVYPLYLTFLILATAGV 62 Query: 87 PSAVSKFVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGNSHVTP 146 P A+S+ +AEA A + ++L S V+ + G+++ L+YA +PLI+ +GN H+ Sbjct: 63 PVALSRVIAEALAEGKQGSIGQILARSMVMMGAIGLVLFGLLYASSPLIAKLMGNPHLIE 122 Query: 147 ALRSSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTSIHADX 206 +R+ + L VPL+A +RG+F G M+ +SQ+ EQT+RV ++ LYL S+ D Sbjct: 123 PIRAISMSLLFVPLIAVIRGFFYGHQKMLFVGLSQIVEQTLRVVFILVASLYLVSLGEDT 182 Query: 207 XXXXXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLALEN 266 + L + H R+ + GG W +L Sbjct: 183 DTVITGVNFGTMISTFLSLGFLALLMWLHNRK--------NSVFGGAEWR------SLTW 228 Query: 267 RRERIRIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAAAMAQ--FGIYNRG 324 +R + P+ + AL +P+ L D F + + A FGIY+RG Sbjct: 229 WYDRAFFASMWRIAWPICISALVIPIFSLTDSFLAINIFRYIWNVDGLTADTWFGIYSRG 288 Query: 325 LPLVQIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXXXXX 384 PL+Q+ ++ +S+++ +PA+AEA + D I T LSLR+ W Sbjct: 289 GPLLQMASLFGSSIALSIVPAIAEAIRQKDQERITTLTKLSLRFAWLIGLPAGLGLTAVA 348 Query: 385 EPVNIALYGDASGSSTMVWLAFTAAGGTVSIISAALLQGLG 425 E N+ALYGD G+ M L TA ++ + + +LQG+G Sbjct: 349 EGANLALYGDMEGTRAMAILGITAIPLSLLLATNGILQGIG 389 >C2U7X2.1 C2U7X2_BACCE Polysaccharide synthase Length = 533 Score = 186 bits (472), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 119/394 (30%), Positives = 202/394 (51%), Gaps = 20/394 (5%) Query: 33 QGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPSAVSK 92 +GA IL+ A+ ++K++ +IP QN+ GD F IY +YP Y + +A G P +SK Sbjct: 11 RGAIILTIASFVTKVLSAFYRIPYQNIAGDIGFYIYQQIYPFYGFCLILATYGFPVIISK 70 Query: 93 FVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGNSHVTPALRSSA 152 VAE ++E ++ +S G + ++ GA I+V +G+ H+ LR + Sbjct: 71 MVAERLERGKQKEAEEIICVSFWFLLGIGFIGFFTLFFGAETIAVAMGDIHLDKLLRVIS 130 Query: 153 WGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTSIHADXXXXXXX 212 + ++P ++ RGYFQG +NM+PTAVSQV EQT+RV++++ L L+L + D Sbjct: 131 FSFLLMPFLSVARGYFQGFNNMMPTAVSQVIEQTIRVSIIVFLSLFLIAHGFDLYTVGAG 190 Query: 213 XXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLALENRRERIR 272 +++LY+RQ R + AA ++ +R+ ++ Sbjct: 191 AMLGSIAGGLIGIIVLILYMRQDFRSIFFKSAA-----------------RIKGKRKIVK 233 Query: 273 IGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAA-AMAQFGIYNRGLPLVQIV 331 I L GL + + L + I + D + LL G +A A G+Y+RG+PL+Q+ Sbjct: 234 I--LFWQGLAICVSNLVLIFIQMADSVSFYSLLIGAGEQAENAKVLKGVYDRGIPLMQLG 291 Query: 332 TMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXXXXXEPVNIAL 391 T++ TS S+ IP + AK RGDL IQ + L+++ + +P NI L Sbjct: 292 TVVTTSFSLSLIPIITAAKERGDLSFIQEKVKLAMKITFVIGLAAAIGLTCIIQPTNIML 351 Query: 392 YGDASGSSTMVWLAFTAAGGTVSIISAALLQGLG 425 + ++ GS + L+ + ++SI +A++LQGLG Sbjct: 352 FENSDGSDVLSILSLSILFSSLSITTASILQGLG 385 >Q81J92.1 Q81J92_BACCR Polysaccharides export protein Length = 533 Score = 184 bits (466), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 118/394 (29%), Positives = 201/394 (51%), Gaps = 20/394 (5%) Query: 33 QGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPSAVSK 92 +GA IL+ A+ ++K++ +IP QN+ GD F IY +YP Y + +A G P +SK Sbjct: 11 RGAIILTIASFVTKVLSAFYRIPYQNIAGDVGFYIYQQIYPFYGFCLILATYGFPVIISK 70 Query: 93 FVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGNSHVTPALRSSA 152 VAE ++E ++ +S G + ++ GA I+V +G+ H+ LR + Sbjct: 71 MVAERLERGKQKEAEEIICVSFWFLLGIGFIGFFTLFFGAETIAVAMGDIHLDKLLRVIS 130 Query: 153 WGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTSIHADXXXXXXX 212 + ++P ++ RGYFQG +NM+PTAVSQV EQT+RV++++ L L+L + D Sbjct: 131 FSFLLMPFLSVARGYFQGFNNMMPTAVSQVIEQTIRVSIIVFLSLFLIAHGFDLYTVGAG 190 Query: 213 XXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLALENRRERIR 272 +++LY+RQ R + AA ++ +R+ ++ Sbjct: 191 AMLGSIAGGLIGIIVLILYMRQDFRSIFFKSAA-----------------RIKGKRKIVK 233 Query: 273 IGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAA-AMAQFGIYNRGLPLVQIV 331 I L GL + + L + I + D + LL G +A A G+Y+R +PL+Q+ Sbjct: 234 I--LFWQGLAICVSNLVLIFIQMADSVSFYSLLIGAGEQAENAKVLKGVYDRSIPLMQLG 291 Query: 332 TMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXXXXXEPVNIAL 391 T++ TS S+ IP + AK RGDL IQ + L+++ + +P NI L Sbjct: 292 TVVTTSFSLSLIPIITAAKERGDLSFIQEKVKLAMKITFVIGLAAAIGLTCIIQPTNIML 351 Query: 392 YGDASGSSTMVWLAFTAAGGTVSIISAALLQGLG 425 + ++ GS + L+ + ++SI +A++LQGLG Sbjct: 352 FENSDGSDVLSILSLSILFSSLSITTASILQGLG 385 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,302,284,136 Number of extensions: 79522079 Number of successful extensions: 249705 Number of sequences better than 1.0e-05: 966 Number of HSP's gapped: 248252 Number of HSP's successfully gapped: 1025 Length of query: 485 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 345 Effective length of database: 1,741,754,103 Effective search space: 600905165535 Effective search space used: 600905165535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)