BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0027.p (252 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K349.1 B3K349_9BACI Imidazole glycerol phosphate synthase subu... 442 e-122 B1DKX4.1 B1DKX4_9BACL Imidazole glycerol phosphate synthase subu... 430 e-119 Q2B720.1 Q2B720_9BACI Imidazole glycerol phosphate synthase subu... 369 e-100 A6CNE0.1 A6CNE0_9BACI Imidazole glycerol phosphate synthase subu... 363 1e-98 C5D7N8.1 C5D7N8_9BACI Imidazoleglycerol phosphate synthase, cycl... 363 1e-98 >B3K349.1 B3K349_9BACI Imidazole glycerol phosphate synthase subunit hisF EC=4.1.3.- Length = 252 Score = 442 bits (1137), Expect = e-122, Method: Compositional matrix adjust. Identities = 216/252 (85%), Positives = 233/252 (92%) Query: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA 60 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA Sbjct: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA 60 Query: 61 TMVEVVRQTAGEIAIPFTVGGGISTAEDMKRILRAGADKIGINTAAVNNPQLILEGARRF 120 TM EVVR+TAGEIAIPFTVGGGIS+ EDMKRILRAGADKIGINTAAV P+LI GAR+F Sbjct: 61 TMEEVVRRTAGEIAIPFTVGGGISSVEDMKRILRAGADKIGINTAAVKQPELISAGARKF 120 Query: 121 GAQCIVVAMDAKYNEAWGEWEVYTHGGRKPTGIRALAWAKEAEKLGAGEILLTSMDADGT 180 G+QCIVVA+DAKYN+AWGEWEVYTHGGR P+GI+AL W K+AE LGAGEILLTSMDADGT Sbjct: 121 GSQCIVVAIDAKYNDAWGEWEVYTHGGRMPSGIKALDWVKQAESLGAGEILLTSMDADGT 180 Query: 181 KDGFDLKLTAAVSDLLTIPVIASGGAGKMEHFYDVFTEGKADAGLAATIFHYKEIAIPDL 240 KDGFDL LT+AV+ + IPVIASGGAGK +HFYDVFT+GKADAGLAATIFHYKEI+IP L Sbjct: 181 KDGFDLPLTSAVASTVQIPVIASGGAGKEDHFYDVFTQGKADAGLAATIFHYKEISIPHL 240 Query: 241 KADLKQKGVEIR 252 K L QKGV+IR Sbjct: 241 KLQLSQKGVDIR 252 >B1DKX4.1 B1DKX4_9BACL Imidazole glycerol phosphate synthase subunit hisF EC=4.1.3.- Length = 252 Score = 430 bits (1106), Expect = e-119, Method: Compositional matrix adjust. Identities = 208/252 (82%), Positives = 232/252 (92%) Query: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA 60 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELA+ YD+EGADELVFLDISASVEGRA Sbjct: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELASTYDQEGADELVFLDISASVEGRA 60 Query: 61 TMVEVVRQTAGEIAIPFTVGGGISTAEDMKRILRAGADKIGINTAAVNNPQLILEGARRF 120 TM+EVV++TAGEI IPFTVGGGIS +DMKRILRAGADKI INTAAV NP L+ +GAR+F Sbjct: 61 TMIEVVKRTAGEITIPFTVGGGISHVDDMKRILRAGADKIAINTAAVLNPSLVSDGARKF 120 Query: 121 GAQCIVVAMDAKYNEAWGEWEVYTHGGRKPTGIRALAWAKEAEKLGAGEILLTSMDADGT 180 G+QCIVVA+DAKYNEAWGEWEV+THGGRK TGI+ L WA+E E+LGAGEILLTSMDADGT Sbjct: 121 GSQCIVVAIDAKYNEAWGEWEVFTHGGRKETGIKVLEWAREIERLGAGEILLTSMDADGT 180 Query: 181 KDGFDLKLTAAVSDLLTIPVIASGGAGKMEHFYDVFTEGKADAGLAATIFHYKEIAIPDL 240 KDGFDLKLT AVSDL+ IPVIASGGAG++EHF DVF +G ADAGLAATIFHYKE+ I ++ Sbjct: 181 KDGFDLKLTRAVSDLVGIPVIASGGAGRIEHFSDVFEQGHADAGLAATIFHYKEMTIREV 240 Query: 241 KADLKQKGVEIR 252 K DL+QKGVE+R Sbjct: 241 KDDLRQKGVEVR 252 >Q2B720.1 Q2B720_9BACI Imidazole glycerol phosphate synthase subunit hisF EC=4.1.3.- Length = 252 Score = 369 bits (948), Expect = e-100, Method: Compositional matrix adjust. Identities = 178/252 (70%), Positives = 208/252 (82%) Query: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA 60 ML KRIIPCLDVK+GRVVKG+ FV LRDAGDPVELA YD++GADELVFLDISAS EGR Sbjct: 1 MLTKRIIPCLDVKEGRVVKGIQFVQLRDAGDPVELARFYDQQGADELVFLDISASHEGRK 60 Query: 61 TMVEVVRQTAGEIAIPFTVGGGISTAEDMKRILRAGADKIGINTAAVNNPQLILEGARRF 120 TM EVV+ A E+AIPFTVGGGI+ +DMKRILRAGADK+ +NTAAVNNP LI EGA F Sbjct: 61 TMTEVVKAVASELAIPFTVGGGINALDDMKRILRAGADKVSLNTAAVNNPDLIREGASFF 120 Query: 121 GAQCIVVAMDAKYNEAWGEWEVYTHGGRKPTGIRALAWAKEAEKLGAGEILLTSMDADGT 180 G+QCIVVA+DAKY+ G W VYTHGGRK T + + WA++A +LGAGEILLTSMDADG Sbjct: 121 GSQCIVVAIDAKYDPELGSWRVYTHGGRKETDLEVIEWARQAVELGAGEILLTSMDADGG 180 Query: 181 KDGFDLKLTAAVSDLLTIPVIASGGAGKMEHFYDVFTEGKADAGLAATIFHYKEIAIPDL 240 K+GFDL LT AVS+ +++PVIASGGAG HF D FTEGKADA LAA+IFHYKE ++ ++ Sbjct: 181 KNGFDLSLTKAVSEAVSVPVIASGGAGNSAHFADAFTEGKADAALAASIFHYKETSVSEV 240 Query: 241 KADLKQKGVEIR 252 K LK+KGV +R Sbjct: 241 KQFLKEKGVTVR 252 >A6CNE0.1 A6CNE0_9BACI Imidazole glycerol phosphate synthase subunit hisF EC=4.1.3.- Length = 252 Score = 363 bits (931), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 173/252 (68%), Positives = 211/252 (83%) Query: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA 60 ML KRIIPCLDVK+GRVVKG++FV LRDAGDPVELA YD++GADELVFLDISAS EGR Sbjct: 1 MLTKRIIPCLDVKEGRVVKGISFVQLRDAGDPVELAKFYDKQGADELVFLDISASHEGRK 60 Query: 61 TMVEVVRQTAGEIAIPFTVGGGISTAEDMKRILRAGADKIGINTAAVNNPQLILEGARRF 120 TM++VV++ A E+AIPFTVGGGI++ EDMK ILRAGADK+ +NTAAV P+LI EG++ F Sbjct: 61 TMIDVVKEVASELAIPFTVGGGINSVEDMKNILRAGADKVSLNTAAVVRPELISEGSQYF 120 Query: 121 GAQCIVVAMDAKYNEAWGEWEVYTHGGRKPTGIRALAWAKEAEKLGAGEILLTSMDADGT 180 G+QCIVVA+DAK+ E G W+VYTHGGRK T A++WA+EAEKLGAGEILLTSMD+DG Sbjct: 121 GSQCIVVAIDAKFEEGSGTWKVYTHGGRKVTEKDAVSWAQEAEKLGAGEILLTSMDSDGG 180 Query: 181 KDGFDLKLTAAVSDLLTIPVIASGGAGKMEHFYDVFTEGKADAGLAATIFHYKEIAIPDL 240 K GF+L LT AVS+ ++IPVIASGGAG EHF ++F GKADA LAA+IFHYKE ++ + Sbjct: 181 KTGFNLSLTKAVSEAVSIPVIASGGAGNAEHFEEIFKRGKADAALAASIFHYKETSVEQV 240 Query: 241 KADLKQKGVEIR 252 K L++K V +R Sbjct: 241 KEYLREKEVAVR 252 >C5D7N8.1 C5D7N8_9BACI Imidazoleglycerol phosphate synthase, cyclase subunit Length = 252 Score = 363 bits (931), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 174/252 (69%), Positives = 207/252 (82%) Query: 1 MLAKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVELAALYDREGADELVFLDISASVEGRA 60 M+ KRIIPCLDVKDGRVVKGV FV LRDAGDPVELA YD++GADELVFLDISAS EGR Sbjct: 1 MITKRIIPCLDVKDGRVVKGVQFVQLRDAGDPVELAKFYDKQGADELVFLDISASHEGRK 60 Query: 61 TMVEVVRQTAGEIAIPFTVGGGISTAEDMKRILRAGADKIGINTAAVNNPQLILEGARRF 120 TMVEVV + A ++AIPFTVGGGI++ +DMK ILRAGADK+ +NTAAV NP LI EGA F Sbjct: 61 TMVEVVEKVAAQLAIPFTVGGGINSLDDMKTILRAGADKVSLNTAAVRNPNLITEGADFF 120 Query: 121 GAQCIVVAMDAKYNEAWGEWEVYTHGGRKPTGIRALAWAKEAEKLGAGEILLTSMDADGT 180 G+QCIVVA+DAKY+E G W VYTHGGR T + + WAKEA + GAGEILLTSMD DG Sbjct: 121 GSQCIVVAIDAKYDETIGSWRVYTHGGRNATDLEVVEWAKEAVRRGAGEILLTSMDCDGE 180 Query: 181 KDGFDLKLTAAVSDLLTIPVIASGGAGKMEHFYDVFTEGKADAGLAATIFHYKEIAIPDL 240 K+GFD+ LT VS+ +++PVIASGGAG +HF +VF +GKADA LAA+IFHYKE ++ ++ Sbjct: 181 KNGFDIALTRTVSEAVSVPVIASGGAGNAKHFLEVFEKGKADAALAASIFHYKETSVKEV 240 Query: 241 KADLKQKGVEIR 252 KA LK++GV IR Sbjct: 241 KAYLKERGVNIR 252 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,503,439,433 Number of extensions: 63734755 Number of successful extensions: 196155 Number of sequences better than 1.0e-05: 2687 Number of HSP's gapped: 191460 Number of HSP's successfully gapped: 2693 Length of query: 252 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 120 Effective length of database: 1,817,122,223 Effective search space: 218054666760 Effective search space used: 218054666760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)