BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0025.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K351.1 B3K351_9BACI Histidinol phosphate phosphatase HisJ fami... 400 e-110 B1DKX6.1 B1DKX6_9BACL Histidinol phosphate phosphatase HisJ fami... 362 3e-98 A4J2A1.1 A4J2A1_DESRM Histidinol-phosphate phosphatase EC=3.1.3.15 231 4e-59 Q67L87.1 Q67L87_SYMTH Histidinol phosphatase 218 3e-55 A6Q755.1 A6Q755_SULNB Histidinol-phosphatase, PHP family EC=3.1.... 218 5e-55 >B3K351.1 B3K351_9BACI Histidinol phosphate phosphatase HisJ family EC=3.1.3.15 Length = 279 Score = 400 bits (1027), Expect = e-110, Method: Compositional matrix adjust. Identities = 188/271 (69%), Positives = 228/271 (84%), Gaps = 1/271 (0%) Query: 1 MRIDYHTHHERCGHAVGTLEEYVQRGIELGLEQLGLSDHLPLIHVDPESYYPEMAMPLSE 60 M IDYHTHH RCGHA G LEEYV+R IELG++QLGLSDH+PLIHV+PE+YYPEMAMP+ E Sbjct: 1 MHIDYHTHHARCGHAEGQLEEYVRRAIELGMDQLGLSDHMPLIHVNPEAYYPEMAMPMDE 60 Query: 61 LPRYVEECLNLKERYRGRIELRVGLEADYIEGYEEQIRELLSPYPWDYLIGSVHFLGEWD 120 LPRYVEECL LKERYRG+I++R+GLE DYIEGYEEQI ++ YPWDY+IGSVHFLGEWD Sbjct: 61 LPRYVEECLQLKERYRGQIDIRIGLEGDYIEGYEEQIERIVKDYPWDYVIGSVHFLGEWD 120 Query: 121 ITDFRQTGGWEGRDVMEVYRRYYDAVRKSALSGLYDIIGHMDVIKRFGYGPQTQEGKAEV 180 ITD+RQT GWEG+D +EVYR+YYDAV+K+A +G YDI+GH+DVIKRFGYGP ++ K++V Sbjct: 121 ITDYRQTQGWEGKDPLEVYRQYYDAVQKAAATGFYDIMGHLDVIKRFGYGPGPEQ-KSDV 179 Query: 181 RALELEALKAIAGSGIAMELNASGLTKPCAEMFPAEHVLQQALELGIPLTLGSDAHDPLK 240 ALE L A+A SG AMELNASGL+K C EMFP+ +L++A++LGIPLTLGSDAH P+K Sbjct: 180 IALEQATLAAVARSGKAMELNASGLSKACEEMFPSRRMLEEAIKLGIPLTLGSDAHQPMK 239 Query: 241 LGDGLQEARSLLWRTGFRRLAVFEGRVRTLV 271 LG+ L +AR+LL G R LA F R R +V Sbjct: 240 LGEHLDQARALLSELGVRELATFRNRKREMV 270 >B1DKX6.1 B1DKX6_9BACL Histidinol phosphate phosphatase HisJ family EC=3.1.3.15 Length = 268 Score = 362 bits (928), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 171/273 (62%), Positives = 216/273 (79%), Gaps = 5/273 (1%) Query: 1 MRIDYHTHHERCGHAVGTLEEYVQRGIELGLEQLGLSDHLPLIHVDPESYYPEMAMPLSE 60 M IDYHTHH RCGHAVG+LEEYVQ+GIE+GL Q+GLSDH+PL+HVDP +YYPEMAMP+ E Sbjct: 1 MFIDYHTHHARCGHAVGSLEEYVQKGIEIGLAQIGLSDHMPLLHVDPATYYPEMAMPMEE 60 Query: 61 LPRYVEECLNLKERYRGRIELRVGLEADYIEGYEEQIRELLSPYPWDYLIGSVHFLGEWD 120 LPRYVEECL LKE+Y+ +I++RVGLE DYIEG EE+I +++ YP+DY+IGSVHFLGEWD Sbjct: 61 LPRYVEECLALKEKYKDQIDIRVGLEGDYIEGQEEKIETIINAYPFDYVIGSVHFLGEWD 120 Query: 121 ITDFRQTGGWEGRDVMEVYRRYYDAVRKSALSGLYDIIGHMDVIKRFGYGPQTQEGKAEV 180 ITDFRQT GWEG++ M+VY +YY+AV K+A +G YD IGH+DVIKRFG+ P+ +V Sbjct: 121 ITDFRQTHGWEGKNRMKVYEQYYEAVAKAAATGFYDYIGHIDVIKRFGFKPE-----GDV 175 Query: 181 RALELEALKAIAGSGIAMELNASGLTKPCAEMFPAEHVLQQALELGIPLTLGSDAHDPLK 240 LE AL+A+ G+A+ELNASGL P EMFP+ +L+ A +L IP+T+GSDAH P + Sbjct: 176 THLENAALEAVKRHGMAIELNASGLRMPVEEMFPSRRMLEYAKQLSIPITIGSDAHQPER 235 Query: 241 LGDGLQEARSLLWRTGFRRLAVFEGRVRTLVPF 273 LG L +AR++L GF +LA FE R LV F Sbjct: 236 LGQYLDQARAVLKDVGFSQLATFERRKLVLVDF 268 >A4J2A1.1 A4J2A1_DESRM Histidinol-phosphate phosphatase EC=3.1.3.15 Length = 268 Score = 231 bits (590), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 109/271 (40%), Positives = 176/271 (64%), Gaps = 6/271 (2%) Query: 1 MRIDYHTHHERCGHAVGTLEEYVQRGIELGLEQLGLSDHLPLIHVDPESYYPEMAMPLSE 60 M +DYH H RCGHAVGT+ EYV + L ++G S+HLPL + E P+ AM E Sbjct: 1 MIVDYHIHTARCGHAVGTVIEYVAEAKKNKLAEIGFSEHLPLYWIPQEKREPDFAMSDEE 60 Query: 61 LPRYVEECLNLKERYRGRIELRVGLEADYIEGYEEQIRELLSPYPWDYLIGSVHFLGEWD 120 LP Y+ E + K+ + + +++G+EADYI GYE++++++L+ P DY++GSVHFLG+W Sbjct: 61 LPIYISEVIQAKKDHPD-LSIKLGIEADYIPGYEQELKKILASMPLDYVLGSVHFLGDWA 119 Query: 121 ITDFRQTGGWEGRDVMEVYRRYYDAVRKSALSGLYDIIGHMDVIKRFGYGPQTQEGKAEV 180 D + ++ D+ E+Y+RY+ V+++A SGL+DII H D+IK+FG+ P T + Sbjct: 120 FDDPDKIDQYQRHDINELYQRYFLQVQQAAASGLFDIISHPDLIKKFGFKPTTSNTE--- 176 Query: 181 RALELEALKAIAGSGIAMELNASGLTKPCAEMFPAEHVLQQALELGIPLTLGSDAHDPLK 240 L + ++ I + + +++NA+G PCAE++P+ L+ G+P+TLGSDAH P + Sbjct: 177 --LYRKTIQVIKANDLCVDVNAAGWRYPCAELYPSPEFLKLCYNYGVPVTLGSDAHKPEQ 234 Query: 241 LGDGLQEARSLLWRTGFRRLAVFEGRVRTLV 271 +G+GL+ A ++L G+R++A F R R +V Sbjct: 235 VGEGLERAVAILKEIGYRQVATFRARKRIMV 265 >Q67L87.1 Q67L87_SYMTH Histidinol phosphatase Length = 271 Score = 218 bits (556), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 119/270 (44%), Positives = 166/270 (61%), Gaps = 6/270 (2%) Query: 4 DYHTHHERCGHAVGTLEEYVQRGIELGLEQLGLSDHLPLIHVDPESYYPEMAMPLSELPR 63 DYH H RCGHAVG +E YV+ I GL ++G SDH+P+ + E P AM + EL Sbjct: 7 DYHMHTVRCGHAVGAMEAYVETAIARGLGEIGFSDHVPMYWLPEERRDPTGAMAMEELEA 66 Query: 64 YVEECLNLKERYRGRIELRVGLEADYIEGYEEQIRELLSPYPWDYLIGSVHFLGEWDITD 123 YV + L L+ERY I +R+GLEAD+I G+EE++ LL PYPWDY+IGSVHFL +W+ Sbjct: 67 YVSDVLRLRERY-PEIPIRLGLEADFIPGHEEELVRLLEPYPWDYVIGSVHFLDDWNFDH 125 Query: 124 FRQTGGWEGRDVMEVYRRYYDAVRKSALSGLYDIIGHMDVIKRFGYGPQTQEGKAEVRAL 183 + D+ +Y R++ R +A SGL+DI+ H+D+IK+FG+ P+ + +A Sbjct: 126 PDLVHRYAEWDIDVLYDRFFALERAAAESGLFDILAHIDLIKKFGHRPRGDQSEAYA--- 182 Query: 184 ELEALKAIAGSGIAMELNASGLTKPCAEMFPAEHVLQQALELGIPLTLGSDAHDPLKLGD 243 AIA SG+A+EL+ +GL KP E +PA +L+ GIP+ + SDAH P ++G Sbjct: 183 --ALADAIARSGVAIELSTAGLRKPVGEYYPAPALLKACCARGIPVVISSDAHAPEEVGW 240 Query: 244 GLQEARSLLWRTGFRRLAVFEGRVRTLVPF 273 G EAR+L GF A F GR R +VP Sbjct: 241 GFAEARALALEVGFTHTARFAGRRRQMVPL 270 >A6Q755.1 A6Q755_SULNB Histidinol-phosphatase, PHP family EC=3.1.3.15 Length = 263 Score = 218 bits (555), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 109/272 (40%), Positives = 170/272 (62%), Gaps = 14/272 (5%) Query: 2 RIDYHTHHERCGHAVGTLEEYVQRGIELGLEQLGLSDHLPLIHVDPESYYPEMAMPLSEL 61 RID H H RC HA GT++EY+Q+ I+LG++ G S+H P+ + + +PL ++ Sbjct: 6 RIDLHNHTARCNHAEGTIDEYIQKAIDLGIDIYGFSEHAPM------DFDEKYRLPLCDM 59 Query: 62 PRYVEECLNLKERYRGRIELRVGLEADYIEGYEEQIRELLSPYPWDYLIGSVHFLGEWDI 121 YV + L+ KERY+ +I++ +G E D++ G+ E E + DYLIGSVHF+ +W Sbjct: 60 DAYVNDVLSAKERYKDQIKILLGYEVDWLPGHME---ENVLNADVDYLIGSVHFIDKWSF 116 Query: 122 TDFRQTGGWEGRDVMEVYRRYYDAVRKSALSGLYDIIGHMDVIKRFGYGPQTQEGKAEVR 181 + GGW+ RD+ E+++ Y++A + A SG +DI+GH+D+IK F Y P+T + R Sbjct: 117 DNPEFIGGWKNRDIDEIWQAYFEATKAMADSGKFDIVGHLDLIKVFKYLPKT-----DTR 171 Query: 182 ALELEALKAIAGSGIAMELNASGLTKPCAEMFPAEHVLQQALELGIPLTLGSDAHDPLKL 241 L EAL++I S + +ELN +GL KP E++P++ +LQ+A L IP+T SDAH ++ Sbjct: 172 ILAKEALQSIKKSKMVIELNTAGLRKPIGEIYPSKALLQEAYALDIPITFSSDAHSVEQV 231 Query: 242 GDGLQEARSLLWRTGFRRLAVFEGRVRTLVPF 273 G G ++A +L G+ + FEGR R LV F Sbjct: 232 GFGYEKAVALAKEVGYTKAVTFEGRDRELVIF 263 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.141 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,741,344,722 Number of extensions: 75825244 Number of successful extensions: 172236 Number of sequences better than 1.0e-05: 510 Number of HSP's gapped: 171157 Number of HSP's successfully gapped: 510 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)