BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0021.p (564 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65M65.1 THIC_BACLD Thiamine biosynthesis protein thiC 941 0.0 P45740.3 THIC_BACSU Thiamine biosynthesis protein thiC 926 0.0 B1HY74.1 B1HY74_LYSSC Thiamine biosynthesis protein 926 0.0 A7Z2P5.1 THIC_BACA2 Thiamine biosynthesis protein thiC 922 0.0 Q9KBJ4.1 THIC_BACHD Thiamine biosynthesis protein thiC 904 0.0 >Q65M65.1 THIC_BACLD Thiamine biosynthesis protein thiC Length = 591 Score = 941 bits (2433), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/562 (78%), Positives = 494/562 (87%), Gaps = 1/562 (0%) Query: 3 DSVKKEEVN-LSGFPASRKVYVEGSRPDIQVPMREICLSRTEGVAGDSDNAPLRVYDTSG 61 +SV+++ ++ +S F SRKVYVEGSRPDIQVPMREI LS T G GD +N P+RVYDTSG Sbjct: 4 ESVQQKNISIMSSFSGSRKVYVEGSRPDIQVPMREISLSPTTGSFGDEENQPVRVYDTSG 63 Query: 62 IYTDDGQETDIRKGLPPHRLCWIAERGDSEEYAGRAVQPGDNGRTVDGLPEEGYSGLKRR 121 YTD + DI KGL P R WI ERGD+EEY GR ++P DNG Y+GLKR+ Sbjct: 64 PYTDANADVDILKGLKPLRATWIRERGDTEEYEGREIRPEDNGYKGKEKASHSYTGLKRK 123 Query: 122 PLRAREGRNVTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPESE 181 PLRA++G+NVTQLHYAR+GIITPEME+IAIRE+ PEFVRDEVA+GRAIIPSN+NHPESE Sbjct: 124 PLRAKKGQNVTQLHYARKGIITPEMEFIAIREHVSPEFVRDEVASGRAIIPSNINHPESE 183 Query: 182 PMIIGRNFLVKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWII 241 PMIIGRNF VKINANIGNSAV+SSI+EEVEKM WA RWGADT+MDLSTG IH TREWII Sbjct: 184 PMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREWII 243 Query: 242 RNSPVPIGTVPIYQALEKVKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRYIPLT 301 RN PVP+GTVPIYQALEKV G+AEDLTW++YRDTLIEQAEQGVDYFTIHAGVL RY+PLT Sbjct: 244 RNCPVPVGTVPIYQALEKVNGVAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLT 303 Query: 302 AGRMTGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIADA 361 A R+TGIVSRGG+IMA WCLAH +E+FLYTHFEEICEIMKTYD+AFSLGDGLRPGSIADA Sbjct: 304 AKRVTGIVSRGGAIMAQWCLAHHEESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADA 363 Query: 362 NDEAQFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLGP 421 NDEAQF WKHDVQVM+EGPGHVPMH+IKEN+DKQ+E+C+EAPFYTLGP Sbjct: 364 NDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMHKIKENVDKQMEICKEAPFYTLGP 423 Query: 422 LTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHAA 481 LTTDIAPGYDHITSAIGAAMIGW+GTAMLCYVTPKEHLGLPNK+DVREGVITYKIAAHAA Sbjct: 424 LTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAA 483 Query: 482 DLAKGHPGAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCSMC 541 DLAKGHPGAQ RD+ALSKARFEFRWRDQF+LSLDPERALEYHDETLPAEGAKTAHFCSMC Sbjct: 484 DLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMC 543 Query: 542 GPKFCSMRISHDIRNSSRLSRM 563 GPKFCSMRIS DIR+ ++ + + Sbjct: 544 GPKFCSMRISQDIRDYAKENNL 565 >P45740.3 THIC_BACSU Thiamine biosynthesis protein thiC Length = 590 Score = 926 bits (2393), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/559 (78%), Positives = 488/559 (87%), Gaps = 2/559 (0%) Query: 2 SDSVKKEEVN-LSGFPASRKVYVEGSRPDIQVPMREICLSRTEGVAGDSDNAPLRVYDTS 60 ++SV++ ++ +S F S+KVYVEGS DIQVPMREI LS T G G+ +NAP+RVYDTS Sbjct: 3 NNSVQQANISIMSSFSGSKKVYVEGSSSDIQVPMREIALSPTTGSFGEEENAPVRVYDTS 62 Query: 61 GIYTDDGQETDIRKGLPPHRLCWIAERGDSEEYAGRAVQPGDNGRTVDGLPEEGYSGLKR 120 G YTD +I++GL P R WI ERGD EEY GRA++P DNG P Y GLKR Sbjct: 63 GPYTDPEVTINIQEGLKPLRQKWITERGDVEEYEGRAIKPEDNGYK-KAKPNVSYPGLKR 121 Query: 121 RPLRAREGRNVTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPES 180 +PLRA+ G+NVTQ+HYA++GIITPEME+IAIRE+ PEFVRDEVA+GRAIIPSN+NHPES Sbjct: 122 KPLRAKAGQNVTQMHYAKKGIITPEMEFIAIREHVSPEFVRDEVASGRAIIPSNINHPES 181 Query: 181 EPMIIGRNFLVKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWI 240 EPMIIGRNF VKINANIGNSAV+SSI+EEVEKM WA RWGADT+MDLSTG IH TREWI Sbjct: 182 EPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREWI 241 Query: 241 IRNSPVPIGTVPIYQALEKVKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRYIPL 300 IRN PVP+GTVPIYQALEKV G+AEDLTWE+YRDTLIEQAEQGVDYFTIHAGVL RY+PL Sbjct: 242 IRNCPVPVGTVPIYQALEKVNGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPL 301 Query: 301 TAGRMTGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIAD 360 TA R TGIVSRGG+IMA WCLAH QE+FLYTHFEEICEIMK YD+AFSLGDGLRPGSIAD Sbjct: 302 TAKRTTGIVSRGGAIMAQWCLAHHQESFLYTHFEEICEIMKMYDIAFSLGDGLRPGSIAD 361 Query: 361 ANDEAQFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLG 420 ANDEAQF WKHDVQVM+EGPGHVPMH+IKEN+DKQ+++C+EAPFYTLG Sbjct: 362 ANDEAQFAELETLGELTQIAWKHDVQVMIEGPGHVPMHKIKENVDKQMDICKEAPFYTLG 421 Query: 421 PLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHA 480 PLTTDIAPGYDHITSAIGAAMIGW+GTAMLCYVTPKEHLGLPN++DVREGVITYKIAAHA Sbjct: 422 PLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAHA 481 Query: 481 ADLAKGHPGAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCSM 540 ADLAKGHPGAQ RD+ALSKARFEFRWRDQF+LSLDPERALEYHDETLPAEGAKTAHFCSM Sbjct: 482 ADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSM 541 Query: 541 CGPKFCSMRISHDIRNSSR 559 CGPKFCSMRIS DIR+ ++ Sbjct: 542 CGPKFCSMRISQDIRDYAK 560 >B1HY74.1 B1HY74_LYSSC Thiamine biosynthesis protein Length = 592 Score = 926 bits (2392), Expect = 0.0, Method: Compositional matrix adjust. Identities = 446/562 (79%), Positives = 480/562 (85%), Gaps = 3/562 (0%) Query: 1 MSDSVKKEEVNLSGFPASRKVYVEGSRPDIQVPMREICLSRTEGVAGDSDNAPLRVYDTS 60 MS +K + +S F S+KVYVEGSRPDI VPMREI LS T G GD +NAP+RVYDTS Sbjct: 1 MSAISEKNIMIMSNFEGSKKVYVEGSRPDILVPMREITLSPTTGSFGDEENAPIRVYDTS 60 Query: 61 GIYTDDGQETDIRKGLPPHRLCWIAERGDSEEYAGRAVQPGDNG-RTVDG--LPEEGYSG 117 G YTD DI KGLP R WI ERGD EEY GR ++P DNG R D + E + Sbjct: 61 GPYTDPAFHVDITKGLPALRSTWIQERGDVEEYEGRTIKPEDNGFRRADDPRIQENVFPE 120 Query: 118 LKRRPLRAREGRNVTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNH 177 L R PLRA+ G+NVTQLHYAR+GIITPEME++AIREN P+FVR E+AAGRAI+PSN+NH Sbjct: 121 LMRMPLRAKRGKNVTQLHYARKGIITPEMEFVAIRENMDPDFVRSEIAAGRAIMPSNINH 180 Query: 178 PESEPMIIGRNFLVKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATR 237 PE+EPMIIGRNF VKINANIGNSAVSSSI EEVEKM WATRWGAD IMDLSTG IH TR Sbjct: 181 PEAEPMIIGRNFHVKINANIGNSAVSSSIAEEVEKMTWATRWGADNIMDLSTGKHIHTTR 240 Query: 238 EWIIRNSPVPIGTVPIYQALEKVKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRY 297 EWIIRN+ VP+GTVPIYQALEKV G+AEDLTWE+YRDTLIEQAEQGVDYFTIHAGVL RY Sbjct: 241 EWIIRNAAVPVGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRY 300 Query: 298 IPLTAGRMTGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGS 357 +PLTA R+TGIVSRGGSIMA WCL H QENFLYTHFEEICEIMKTYDVAFSLGDGLRPGS Sbjct: 301 VPLTANRVTGIVSRGGSIMAQWCLYHHQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGS 360 Query: 358 IADANDEAQFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFY 417 IADANDEAQF WKHDVQVMVEGPGHVPMH IKENMDKQLE+C+EAPFY Sbjct: 361 IADANDEAQFAELETLGELTQIAWKHDVQVMVEGPGHVPMHLIKENMDKQLEVCKEAPFY 420 Query: 418 TLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIA 477 TLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPN+ DVR GVITYKIA Sbjct: 421 TLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNREDVRVGVITYKIA 480 Query: 478 AHAADLAKGHPGAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHF 537 AHAADLAKGHPGAQ RD+ALSKARFEFRWRDQF+LSLDPERA+EYHDETLPAEGAKTAHF Sbjct: 481 AHAADLAKGHPGAQQRDDALSKARFEFRWRDQFNLSLDPERAVEYHDETLPAEGAKTAHF 540 Query: 538 CSMCGPKFCSMRISHDIRNSSR 559 CSMCGPKFCSMRIS DIRN ++ Sbjct: 541 CSMCGPKFCSMRISQDIRNYAK 562 >A7Z2P5.1 THIC_BACA2 Thiamine biosynthesis protein thiC Length = 590 Score = 922 bits (2383), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/559 (78%), Positives = 486/559 (86%), Gaps = 2/559 (0%) Query: 2 SDSVKKEEVN-LSGFPASRKVYVEGSRPDIQVPMREICLSRTEGVAGDSDNAPLRVYDTS 60 + SV++ ++ +S F S+KVY EGS PDI+VPMREI LS T G G+ +NAP+RVYDTS Sbjct: 3 NQSVQQANISIMSSFSGSKKVYAEGSSPDIRVPMREISLSATTGAFGEEENAPVRVYDTS 62 Query: 61 GIYTDDGQETDIRKGLPPHRLCWIAERGDSEEYAGRAVQPGDNGRTVDGLPEEGYSGLKR 120 G YTD + DI +GL R WI RGD EEY GR V+P DNG + E Y GL+R Sbjct: 63 GPYTDPDVQIDIHEGLGALRTKWITGRGDVEEYNGRIVKPEDNGYKKEDHAAE-YPGLQR 121 Query: 121 RPLRAREGRNVTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPES 180 +PLRA+ G+NVTQLHYAR+GIITPEME+IAIRE+ PEFVR+EVA+GRAIIPSN+NHPES Sbjct: 122 KPLRAKPGKNVTQLHYARKGIITPEMEFIAIREHVSPEFVREEVASGRAIIPSNVNHPES 181 Query: 181 EPMIIGRNFLVKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWI 240 EPMIIGRNF VKINANIGNSAV+SSI+EEVEKM WA RWGADT+MDLSTG IH TREWI Sbjct: 182 EPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREWI 241 Query: 241 IRNSPVPIGTVPIYQALEKVKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRYIPL 300 IRN PVP+GTVPIYQALEKV GIAEDLTW++YRDTLIEQAEQGVDYFTIHAGVL RY+PL Sbjct: 242 IRNCPVPVGTVPIYQALEKVNGIAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYVPL 301 Query: 301 TAGRMTGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIAD 360 TA R TGIVSRGG+IMA WCLAH QE+FLYTHFEEICEIMKTYD+AFSLGDGLRPGSIAD Sbjct: 302 TAKRTTGIVSRGGAIMAQWCLAHHQESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIAD 361 Query: 361 ANDEAQFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLG 420 ANDEAQF WKHDVQVM+EGPGHVPMH+IKENMDKQLE+C+EAPFYTLG Sbjct: 362 ANDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMHKIKENMDKQLEICKEAPFYTLG 421 Query: 421 PLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHA 480 PLTTDIAPGYDHITSAIGAAMIGW+GTAMLCYVTPKEHLGLPN++DVREGVITYKIAAHA Sbjct: 422 PLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAHA 481 Query: 481 ADLAKGHPGAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCSM 540 ADLAKGHPGAQ RD+ALSKARFEFRWRDQFHLSLDPERA+EYHDETLPAEGAKTAHFCSM Sbjct: 482 ADLAKGHPGAQIRDDALSKARFEFRWRDQFHLSLDPERAMEYHDETLPAEGAKTAHFCSM 541 Query: 541 CGPKFCSMRISHDIRNSSR 559 CGPKFCSMRIS DIR+ ++ Sbjct: 542 CGPKFCSMRISQDIRDYAK 560 >Q9KBJ4.1 THIC_BACHD Thiamine biosynthesis protein thiC Length = 595 Score = 904 bits (2337), Expect = 0.0, Method: Compositional matrix adjust. Identities = 430/551 (78%), Positives = 473/551 (85%), Gaps = 4/551 (0%) Query: 13 SGFPASRKVYVEGSRPDIQVPMREICLSRTEGVAGDSDNAPLRVYDTSGIYTDDGQETDI 72 S FP S+K YV GSR DI+VP REI T +G+++N P+ VYD+SG YTD+ E +I Sbjct: 15 SSFPNSQKRYVTGSRSDIRVPFREISQHPTVTDSGEAENEPILVYDSSGPYTDESYEVNI 74 Query: 73 RKGLPPHRLCWIAERGDSEEYAGRAVQPGDNGRTVDGLPEEGYSGLKR----RPLRAREG 128 KGLP R WI ERGD E+Y GR V+P DNG T D P R +PLRA++G Sbjct: 75 EKGLPAIRKSWILERGDCEKYEGRDVRPEDNGFTKDDDPRAAREVFPRTSSHKPLRAKKG 134 Query: 129 RNVTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPESEPMIIGRN 188 NVTQ+HYA++GI+TPEME++AIREN PEFVRDE+A GRAI+PSN+NHPESEPMIIGRN Sbjct: 135 ANVTQMHYAKKGIVTPEMEFVAIRENVSPEFVRDELARGRAILPSNINHPESEPMIIGRN 194 Query: 189 FLVKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWIIRNSPVPI 248 F VKINANIGNSAVSSSI EEVEKM WATRWGADTIMDLSTG IH TREWIIRNSPVP+ Sbjct: 195 FHVKINANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRNSPVPV 254 Query: 249 GTVPIYQALEKVKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRYIPLTAGRMTGI 308 GTVPIYQALEKV G+AEDLTWE+YRDTLIEQAEQGVDYFTIHAGVL RYIPLTA R+TGI Sbjct: 255 GTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRVTGI 314 Query: 309 VSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIADANDEAQFX 368 VSRGGSIMA WCL H +ENFLYTHFEEICEIMKTYD+AFSLGDGLRPGSIADANDEAQF Sbjct: 315 VSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADANDEAQFA 374 Query: 369 XXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLGPLTTDIAP 428 W+HDVQVMVEGPGHVPMH+IKENMDKQL++C+EAPFYTLGPLTTDIAP Sbjct: 375 ELETLGELTKIAWEHDVQVMVEGPGHVPMHQIKENMDKQLDICQEAPFYTLGPLTTDIAP 434 Query: 429 GYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHAADLAKGHP 488 GYDHITSAIGAAMIGW+GTAMLCYVTPKEHLGLPNK+DVREGVITYKIAAHAADLAKGHP Sbjct: 435 GYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADLAKGHP 494 Query: 489 GAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSM 548 GAQ RD+ALSKARFEFRWRDQF+LSLDPERA+E+HDETLPAEGAKTAHFCSMCGPKFCSM Sbjct: 495 GAQKRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSM 554 Query: 549 RISHDIRNSSR 559 RIS DIR ++ Sbjct: 555 RISQDIRTYAK 565 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,531,883,379 Number of extensions: 154712927 Number of successful extensions: 315668 Number of sequences better than 1.0e-05: 1143 Number of HSP's gapped: 313336 Number of HSP's successfully gapped: 1240 Length of query: 564 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 423 Effective length of database: 1,732,333,088 Effective search space: 732776896224 Effective search space used: 732776896224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)