BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0011.p (212 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGM3.1 B3KGM3_9BACI Lipolytic protein G-D-S-L family 217 6e-55 Q65NA4.1 LIPC_BACLD Spore germination lipase lipC EC=3.-.-.- 131 3e-29 P42969.1 LIPC_BACSU Spore germination lipase lipC EC=3.-.-.- 122 3e-26 B4AIR0.1 B4AIR0_BACPU Spore germination lipase LipC EC=3.-.-.- 116 1e-24 A8FA14.1 A8FA14_BACP2 Lipolytic enzyme LipC 112 2e-23 >B3KGM3.1 B3KGM3_9BACI Lipolytic protein G-D-S-L family Length = 211 Score = 217 bits (552), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 107/208 (51%), Positives = 144/208 (69%), Gaps = 2/208 (0%) Query: 1 MSYQYTAIGDSLTTGFGALPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLG 60 + Y YTA+GDSLT GFGA+PG+GFVP+YRRMAE +LR+ V NLG+NGLT+ L R+ Sbjct: 2 LVYHYTAVGDSLTFGFGAMPGSGFVPLYRRMAEEKLRQFVAHENLGVNGLTSDELYDRVV 61 Query: 61 SDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGSL 120 P +R ++ A+IIT+SIGGNDLI+A K+ RP L AL C+ + I+ + Sbjct: 62 QFPTYRYHLQQAQIITMSIGGNDLIRAVKSTGGRPN--REVLDTALYRCQHHVANIVKHI 119 Query: 121 RQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGVCGFASIYHEFAGNE 180 R++K + +PY IR +GLYNPYP TEAT++V +FN G S G A IY +F+G E Sbjct: 120 RKIKSPASKPYFIRAIGLYNPYPAWTEATEYVIRFNRHLMGLSDGYFRVADIYAQFSGRE 179 Query: 181 RSLLSLDHVHPNGRGYRVIAGKLDSLGY 208 + L+S+D +HPNGRGYRVIA +L+ LGY Sbjct: 180 KELMSVDGLHPNGRGYRVIAEQLNRLGY 207 >Q65NA4.1 LIPC_BACLD Spore germination lipase lipC EC=3.-.-.- Length = 212 Score = 131 bits (330), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 72/213 (33%), Positives = 114/213 (53%), Gaps = 5/213 (2%) Query: 1 MSYQYTAIGDSLTTGFGA-LPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 M+ QYTA+GDSLT G GA L GFV Y+R E L E V + +GL T+ + L Sbjct: 1 MTLQYTALGDSLTVGVGAGLFEPGFVQRYKRKMEEDLNEEVSLIVFAKSGLETSEILAML 60 Query: 60 GSDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGS 119 ++P + ++ A++IT++ GNDL+++ + + A C++N+ G++ Sbjct: 61 -NEPFIMEQVKKADVITITGCGNDLLQSLEI--YEKEKDEHVFLEASSHCQKNYSGMLEK 117 Query: 120 LRQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSG-VCGFASIYHEFAG 178 +R++K Y++R++ LYNP+P + A W+ FN S Y F G Sbjct: 118 IREIKGEKDTRYLVRLLNLYNPFPSIELADKWISGFNRHLKQLESAPQIKVIDTYAVFKG 177 Query: 179 NERSLLSLDHVHPNGRGYRVIAGKLDSLGYWRL 211 E+ LS+D VHP+ RGY ++ KL + GY RL Sbjct: 178 REKEYLSIDRVHPSSRGYEAMSEKLRAAGYGRL 210 >P42969.1 LIPC_BACSU Spore germination lipase lipC EC=3.-.-.- Length = 213 Score = 122 bits (305), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 75/215 (34%), Positives = 113/215 (52%), Gaps = 9/215 (4%) Query: 1 MSYQYTAIGDSLTTGFGA-LPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 M +YTA+GDSLTTG G+ L GFV + M E L+ +GL T E+ L Sbjct: 1 MVLRYTALGDSLTTGRGSGLFSPGFVQRFGDMMEADLKTRTAINIFARSGLNT---EEIL 57 Query: 60 G--SDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIM 117 G S P ++ IR+A++IT++ GNDLI + ++++ +R C NF ++ Sbjct: 58 GLLSYPYVQKCIRDADMITITGCGNDLIDSV--LAYQTSKDETIFSRVSAHCHENFEKMI 115 Query: 118 GSLRQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGV-CGFASIYHEF 176 + ++K + PY IR+ LYNP+P++ A W+ FN+ +S A Y F Sbjct: 116 AKVAEIKGENPSPYAIRVFNLYNPFPEIDIAGKWITSFNSHLETLASAPHVKIADAYSIF 175 Query: 177 AGNERSLLSLDHVHPNGRGYRVIAGKLDSLGYWRL 211 G E+ LS D VHPN +GY+ +A + LGY L Sbjct: 176 KGKEQEYLSFDGVHPNSKGYQAMAEAVHKLGYKEL 210 >B4AIR0.1 B4AIR0_BACPU Spore germination lipase LipC EC=3.-.-.- Length = 210 Score = 116 bits (291), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 72/208 (34%), Positives = 103/208 (49%), Gaps = 11/208 (5%) Query: 1 MSYQYTAIGDSLTTGFGA-LPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 M Y AIGDSLT G GA L G GF Y+ M E + PV +GLTT + Q Sbjct: 1 MGRSYVAIGDSLTVGVGARLFGGGFAERYQWMLEKKTHTPVELSVFAKSGLTTDQILQLF 60 Query: 60 GSDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGS 119 + R I G NDL++A + + G+ L +A C+ NF ++ Sbjct: 61 KRSDVRRAIAAADIITITGCG-NDLVQAIQ--QYEKGEDEKKLLQATLHCQANFSKMIQE 117 Query: 120 LRQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFN----AFAAGYSSGVCGFASIYHE 175 + Q+K G +QPY + ++ LYNP+PQ+ A W++Q+N + AA V +Y Sbjct: 118 IAQIKRGQKQPYCVYLMNLYNPFPQIPIAGRWLQQYNHQLRSLAANPHVEV---VDVYRA 174 Query: 176 FAGNERSLLSLDHVHPNGRGYRVIAGKL 203 F G+E LS+D HPN +GY +A L Sbjct: 175 FEGHENEYLSIDQFHPNHKGYEAMAKAL 202 >A8FA14.1 A8FA14_BACP2 Lipolytic enzyme LipC Length = 210 Score = 112 bits (281), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 71/208 (34%), Positives = 102/208 (49%), Gaps = 11/208 (5%) Query: 1 MSYQYTAIGDSLTTGFGA-LPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 M Y AIGDSLT G GA L G GF Y+ M E + PV +GLTT + Q Sbjct: 1 MGRSYVAIGDSLTVGVGARLFGGGFAERYQWMLEKKTHAPVELSVFAKSGLTTDQILQLF 60 Query: 60 GSDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGS 119 + R I G NDL++A + + G+ L +A C+ NF ++ Sbjct: 61 KRSNVRRAIAAANIITITGCG-NDLVQAIQ--QYEKGEDETKLLQATLHCQANFSKMIQE 117 Query: 120 LRQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFN----AFAAGYSSGVCGFASIYHE 175 + Q+K G +Q Y + ++ LYNP+PQ+ A W++Q+N + AA V +Y Sbjct: 118 IAQIKRGQKQSYCVYLMNLYNPFPQIPIAGRWLQQYNHQLRSLAANPHVEV---VDVYRA 174 Query: 176 FAGNERSLLSLDHVHPNGRGYRVIAGKL 203 F G+E LS+D HPN +GY +A L Sbjct: 175 FEGHEHEYLSIDQFHPNHKGYEAMAKIL 202 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,330,569,810 Number of extensions: 54538666 Number of successful extensions: 118614 Number of sequences better than 1.0e-05: 289 Number of HSP's gapped: 118167 Number of HSP's successfully gapped: 289 Length of query: 212 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 82 Effective length of database: 1,835,964,253 Effective search space: 150549068746 Effective search space used: 150549068746 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)