BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0006.p (251 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKZ0.1 B1DKZ0_9BACL Triosephosphate isomerase EC=5.3.1.1 446 e-123 B3K3A0.1 B3K3A0_9BACI Triosephosphate isomerase EC=5.3.1.1 434 e-120 C0Z6L5.1 C0Z6L5_BREBN Triosephosphate isomerase EC=5.3.1.1 340 8e-92 Q9K715.1 TPIS_BACHD Triosephosphate isomerase EC=5.3.1.1 330 9e-89 A7Z8Y3.1 TPIS_BACA2 Triosephosphate isomerase EC=5.3.1.1 327 4e-88 >B1DKZ0.1 B1DKZ0_9BACL Triosephosphate isomerase EC=5.3.1.1 Length = 251 Score = 446 bits (1146), Expect = e-123, Method: Compositional matrix adjust. Identities = 214/251 (85%), Positives = 231/251 (92%) Query: 1 MSRTPIIAGNWKMFKTVSEAEGFFAEVKGRAEVEGVETVICAPFTNLPALVAAAKGTSIK 60 MSRTPIIAGNWKMFKTVSEA FF+EVKG+AEV GVE+VICAP+T LPALV AAKGTSI Sbjct: 1 MSRTPIIAGNWKMFKTVSEAVSFFSEVKGKAEVAGVESVICAPYTTLPALVEAAKGTSIA 60 Query: 61 IGAQNLHFEDNGAYTGEISGVMLKDLGVDYVIIGHSERRAYFGETDEIVNKKMHAAFRHG 120 IGAQNLHFEDNGA+TGEISGVMLKDLGV YVIIGHSERRAYF ETDEIVNKKMH+AF+HG Sbjct: 61 IGAQNLHFEDNGAFTGEISGVMLKDLGVKYVIIGHSERRAYFAETDEIVNKKMHSAFKHG 120 Query: 121 ITPIVCVGEKLEEREADQTKAVCKVQTEGAFAGLSAEQAASVVIAYEPIWAIGTGKSSTS 180 ITPIVCVGEKLEEREA+QTK VCKVQTE AF GLSA QAA VVIAYEPIWAIGTGKSSTS Sbjct: 121 ITPIVCVGEKLEEREANQTKDVCKVQTEAAFEGLSAAQAAEVVIAYEPIWAIGTGKSSTS 180 Query: 181 QDANEVIAYIRTLVKDLYDQATAEAVRIQYGGSVKPENVTEYMSQSDIDGALVGGASLQP 240 +DA +VI YIR++V DLYD ATAEAVRIQYGGSVKP NV EYM Q+DIDGALVGGASL+P Sbjct: 181 EDAQDVIGYIRSVVADLYDAATAEAVRIQYGGSVKPNNVAEYMGQADIDGALVGGASLEP 240 Query: 241 ASFVSLVEGAK 251 AS+++LVEGAK Sbjct: 241 ASYIALVEGAK 251 >B3K3A0.1 B3K3A0_9BACI Triosephosphate isomerase EC=5.3.1.1 Length = 250 Score = 434 bits (1116), Expect = e-120, Method: Compositional matrix adjust. Identities = 209/249 (83%), Positives = 226/249 (90%) Query: 3 RTPIIAGNWKMFKTVSEAEGFFAEVKGRAEVEGVETVICAPFTNLPALVAAAKGTSIKIG 62 RTPIIAGNWKMFKTV EA F +VKG+AEVEGVE+VICAPFTNLPALV AAKGT+IKIG Sbjct: 2 RTPIIAGNWKMFKTVPEATSFIEDVKGKAEVEGVESVICAPFTNLPALVEAAKGTNIKIG 61 Query: 63 AQNLHFEDNGAYTGEISGVMLKDLGVDYVIIGHSERRAYFGETDEIVNKKMHAAFRHGIT 122 AQNLHFEDNGA+TGEISG+MLKDLGVDYVIIGHSERR YF ETDEIVNKK+HAAFRHG+ Sbjct: 62 AQNLHFEDNGAFTGEISGLMLKDLGVDYVIIGHSERRQYFAETDEIVNKKVHAAFRHGLL 121 Query: 123 PIVCVGEKLEEREADQTKAVCKVQTEGAFAGLSAEQAASVVIAYEPIWAIGTGKSSTSQD 182 PIVCVGEKLEEREA QTK VCKVQTE AFAG+ A+QA VVIAYEPIWAIGTGKSST++D Sbjct: 122 PIVCVGEKLEEREAGQTKDVCKVQTEAAFAGVPADQAVHVVIAYEPIWAIGTGKSSTAED 181 Query: 183 ANEVIAYIRTLVKDLYDQATAEAVRIQYGGSVKPENVTEYMSQSDIDGALVGGASLQPAS 242 ANEVIAYIR LVK LY + A+ VRIQYGGSVKPENV EYM QSDIDGALVGGASLQPAS Sbjct: 182 ANEVIAYIRELVKGLYGETVADQVRIQYGGSVKPENVAEYMGQSDIDGALVGGASLQPAS 241 Query: 243 FVSLVEGAK 251 +++LVEGAK Sbjct: 242 YIALVEGAK 250 >C0Z6L5.1 C0Z6L5_BREBN Triosephosphate isomerase EC=5.3.1.1 Length = 252 Score = 340 bits (871), Expect = 8e-92, Method: Compositional matrix adjust. Identities = 165/248 (66%), Positives = 191/248 (77%) Query: 3 RTPIIAGNWKMFKTVSEAEGFFAEVKGRAEVEGVETVICAPFTNLPALVAAAKGTSIKIG 62 RTPIIAGNWKMFKT++EA F E K GV+ VICAPFT L AL GT I IG Sbjct: 2 RTPIIAGNWKMFKTMAEARAFAQEAKASNNTSGVQKVICAPFTALTALKEELAGTDIAIG 61 Query: 63 AQNLHFEDNGAYTGEISGVMLKDLGVDYVIIGHSERRAYFGETDEIVNKKMHAAFRHGIT 122 AQN+HFE+ GAYTGEIS MLK++GV+YVI+GHSERR YF ETDE VNKK+ AA G+ Sbjct: 62 AQNMHFEEQGAYTGEISASMLKEIGVEYVILGHSERRQYFNETDETVNKKVVAALAAGLV 121 Query: 123 PIVCVGEKLEEREADQTKAVCKVQTEGAFAGLSAEQAASVVIAYEPIWAIGTGKSSTSQD 182 PIVCVGE LE+REA +T V + QTEGAFAG+ A Q A VIAYEPIWAIGTGKSST++D Sbjct: 122 PIVCVGESLEQREAGETANVVRTQTEGAFAGVDAAQVADTVIAYEPIWAIGTGKSSTAED 181 Query: 183 ANEVIAYIRTLVKDLYDQATAEAVRIQYGGSVKPENVTEYMSQSDIDGALVGGASLQPAS 242 ANE IA IR+++ + + QATAE VRIQYGGSVKPEN+ YM+Q DIDGALVGGASL S Sbjct: 182 ANETIAIIRSVIGERFGQATAEKVRIQYGGSVKPENIASYMAQPDIDGALVGGASLVANS 241 Query: 243 FVSLVEGA 250 ++ LV GA Sbjct: 242 YLQLVAGA 249 >Q9K715.1 TPIS_BACHD Triosephosphate isomerase EC=5.3.1.1 Length = 251 Score = 330 bits (845), Expect = 9e-89, Method: Compositional matrix adjust. Identities = 159/250 (63%), Positives = 192/250 (76%), Gaps = 1/250 (0%) Query: 3 RTPIIAGNWKMFKTVSEAEGFFAEVKGR-AEVEGVETVICAPFTNLPALVAAAKGTSIKI 61 R PIIAGNWKM KTV EA+ F EVKG + V++V+C+P L LV A+GT ++I Sbjct: 2 RKPIIAGNWKMNKTVGEAKAFVEEVKGAIPSSDKVDSVVCSPALFLEGLVQKAEGTELRI 61 Query: 62 GAQNLHFEDNGAYTGEISGVMLKDLGVDYVIIGHSERRAYFGETDEIVNKKMHAAFRHGI 121 GAQN+HFE++GA+TGEIS V L D+ VDYVI+GHSERR F ETDE+VNKK HAAF HG+ Sbjct: 62 GAQNMHFEESGAFTGEISPVALSDMKVDYVILGHSERRDMFAETDELVNKKTHAAFAHGL 121 Query: 122 TPIVCVGEKLEEREADQTKAVCKVQTEGAFAGLSAEQAASVVIAYEPIWAIGTGKSSTSQ 181 TPIVCVGE LEEREA+QT V K Q E GL+ EQ VIAYEP+WAIGTGKSS+++ Sbjct: 122 TPIVCVGETLEEREANQTYDVVKTQVEKGLEGLTDEQVKVTVIAYEPVWAIGTGKSSSAE 181 Query: 182 DANEVIAYIRTLVKDLYDQATAEAVRIQYGGSVKPENVTEYMSQSDIDGALVGGASLQPA 241 DAN+V +YIR +V + + Q A+AVRIQYGGSVKP N+ EYM+QSDIDGALVGGASL P Sbjct: 182 DANDVCSYIRKVVTEKFSQEAADAVRIQYGGSVKPANIAEYMAQSDIDGALVGGASLDPQ 241 Query: 242 SFVSLVEGAK 251 SF+ L+E K Sbjct: 242 SFLQLLEAVK 251 >A7Z8Y3.1 TPIS_BACA2 Triosephosphate isomerase EC=5.3.1.1 Length = 253 Score = 327 bits (839), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 159/247 (64%), Positives = 189/247 (76%), Gaps = 1/247 (0%) Query: 3 RTPIIAGNWKMFKTVSEAEGFFAEVKGR-AEVEGVETVICAPFTNLPALVAAAKGTSIKI 61 R PIIAGNWKM KT+ EA F EVK + E V+CAP L L +A KGT +K+ Sbjct: 2 RKPIIAGNWKMNKTLGEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLTSAVKGTDLKV 61 Query: 62 GAQNLHFEDNGAYTGEISGVMLKDLGVDYVIIGHSERRAYFGETDEIVNKKMHAAFRHGI 121 GAQN+HFE+NGA+TGEIS V LKDLGVDY +IGHSERR F ETDE VNKK HAAF+HGI Sbjct: 62 GAQNMHFEENGAFTGEISPVALKDLGVDYCVIGHSERREMFAETDETVNKKAHAAFKHGI 121 Query: 122 TPIVCVGEKLEEREADQTKAVCKVQTEGAFAGLSAEQAASVVIAYEPIWAIGTGKSSTSQ 181 PI+CVGE LEEREA +T + Q + AGLS EQ A+ VIAYEPIWAIGTGKSST++ Sbjct: 122 VPIICVGETLEEREAGKTNDLVADQVKKGLAGLSEEQVAASVIAYEPIWAIGTGKSSTAK 181 Query: 182 DANEVIAYIRTLVKDLYDQATAEAVRIQYGGSVKPENVTEYMSQSDIDGALVGGASLQPA 241 DAN+V A+IR +V + + Q TA+ +RIQYGGSVKP N+ EYM++SDIDGALVGGASL+P Sbjct: 182 DANDVCAHIRKVVAESFSQETADKLRIQYGGSVKPANIKEYMAESDIDGALVGGASLEPQ 241 Query: 242 SFVSLVE 248 SFV L+E Sbjct: 242 SFVQLLE 248 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,431,609,617 Number of extensions: 57002332 Number of successful extensions: 120187 Number of sequences better than 1.0e-05: 2823 Number of HSP's gapped: 116271 Number of HSP's successfully gapped: 2823 Length of query: 251 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 119 Effective length of database: 1,817,122,223 Effective search space: 216237544537 Effective search space used: 216237544537 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)