BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0004.p (428 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3A2.1 B3K3A2_9BACI Enolase EC=4.2.1.11 765 0.0 B1DKZ2.1 B1DKZ2_9BACL Enolase EC=4.2.1.11 699 0.0 Q9K717.1 ENO_BACHD Enolase EC=4.2.1.11 645 0.0 A4J8T7.1 A4J8T7_DESRM Enolase EC=4.2.1.11 644 0.0 Q5WDK9.1 ENO_BACSK Enolase EC=4.2.1.11 639 0.0 >B3K3A2.1 B3K3A2_9BACI Enolase EC=4.2.1.11 Length = 429 Score = 765 bits (1976), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/428 (89%), Positives = 390/428 (91%) Query: 1 MTIISDVYAREVLDSRGNPTVEVDVYLESGAKGRAIVPSGASTGAHEAVELRDEDKNRYL 60 MTIISDVYAREVLDSRGNPTVEV+VYLESGA GRAIVPSGASTG HEAVELRD+DK+RYL Sbjct: 1 MTIISDVYAREVLDSRGNPTVEVEVYLESGAIGRAIVPSGASTGVHEAVELRDDDKSRYL 60 Query: 61 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMITLDGTHNKGKLGANXXXXXXXXXXX 120 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMI LDGT NKGKLGAN Sbjct: 61 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMIALDGTPNKGKLGANAILAVSMAVAR 120 Query: 121 XXXXXXXXXXXXXXGGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR 180 GGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR Sbjct: 121 AAADALDLPLYVYLGGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR 180 Query: 181 TGAEIFHALKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSIMEAIEKAGYKPGVDVFLG 240 GAEIFH LKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSI+EAIEKAGYKPG DV LG Sbjct: 181 MGAEIFHNLKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSIIEAIEKAGYKPGEDVLLG 240 Query: 241 MDVASTEFFKDGKYHLEGEGKSFTPAEFVDLLTSWADKYPIITIEDGCSEDDWEGWKLLT 300 MDVASTEFFKDGKY LEGEGKSFTPAEFVDLL SW DKYPI+TIEDGCSEDDWEGWKLLT Sbjct: 241 MDVASTEFFKDGKYVLEGEGKSFTPAEFVDLLASWVDKYPIVTIEDGCSEDDWEGWKLLT 300 Query: 301 EKLGNRIQLVGDDLFVTNTERLNKGIEEGIGNSILIKVNQIGTLTETFDAIEMAKRAGYT 360 EKLGN+IQLVGDDLFVTNTERL KGI EGIGNSILIKVNQIGTLTETFDAIEMAKRAGYT Sbjct: 301 EKLGNKIQLVGDDLFVTNTERLEKGINEGIGNSILIKVNQIGTLTETFDAIEMAKRAGYT 360 Query: 361 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRIAKYNQLLRIEDELGELAQYNGL 420 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDR+AKYNQLLRIED+LGELAQYNGL Sbjct: 361 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLGELAQYNGL 420 Query: 421 KSFYNLKR 428 KSFYNLK+ Sbjct: 421 KSFYNLKQ 428 >B1DKZ2.1 B1DKZ2_9BACL Enolase EC=4.2.1.11 Length = 430 Score = 699 bits (1804), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/427 (80%), Positives = 372/427 (87%) Query: 1 MTIISDVYAREVLDSRGNPTVEVDVYLESGAKGRAIVPSGASTGAHEAVELRDEDKNRYL 60 M+II DVYAREVLDSRGNPTVEV+V LESG KGRAIVPSGASTGA+EAVELRD DK RYL Sbjct: 1 MSIIVDVYAREVLDSRGNPTVEVEVSLESGGKGRAIVPSGASTGAYEAVELRDGDKGRYL 60 Query: 61 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMITLDGTHNKGKLGANXXXXXXXXXXX 120 GKGV KAVENVN IIAPE+IG+DALDQ+ ID+ MI LDGT NK KLGAN Sbjct: 61 GKGVEKAVENVNAIIAPEIIGLDALDQVAIDRKMIELDGTPNKAKLGANAILAVSMAVAR 120 Query: 121 XXXXXXXXXXXXXXGGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR 180 GGFNAK LPVPMMNI+NGG HADNN+DVQEFMVLPVGAP+FKEALR Sbjct: 121 AAADALNVSLYTYLGGFNAKTLPVPMMNIINGGEHADNNIDVQEFMVLPVGAPTFKEALR 180 Query: 181 TGAEIFHALKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSIMEAIEKAGYKPGVDVFLG 240 GAEIFH LKSVLK KGLNTAVGDEGGFAPN SNE+A+++I+ AIE AGYKPGVDVFLG Sbjct: 181 IGAEIFHNLKSVLKDKGLNTAVGDEGGFAPNLGSNEEAITTIIAAIELAGYKPGVDVFLG 240 Query: 241 MDVASTEFFKDGKYHLEGEGKSFTPAEFVDLLTSWADKYPIITIEDGCSEDDWEGWKLLT 300 MDVASTEF+KDGKYHLEGEGKSFT AEFVDLL SW +KYPIITIEDGCSEDDWEGWKLLT Sbjct: 241 MDVASTEFYKDGKYHLEGEGKSFTSAEFVDLLASWVEKYPIITIEDGCSEDDWEGWKLLT 300 Query: 301 EKLGNRIQLVGDDLFVTNTERLNKGIEEGIGNSILIKVNQIGTLTETFDAIEMAKRAGYT 360 +KLG ++QLVGDDLFVTNTERL+ GIE+G+GNSIL+KVNQIG+LTETFDAIEMAKRAGYT Sbjct: 301 DKLGGKVQLVGDDLFVTNTERLSDGIEKGVGNSILVKVNQIGSLTETFDAIEMAKRAGYT 360 Query: 361 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRIAKYNQLLRIEDELGELAQYNGL 420 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDR+AKYNQLLRIED+LG +AQY G Sbjct: 361 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLGSVAQYAGK 420 Query: 421 KSFYNLK 427 +FYNLK Sbjct: 421 SAFYNLK 427 >Q9K717.1 ENO_BACHD Enolase EC=4.2.1.11 Length = 429 Score = 645 bits (1664), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/430 (74%), Positives = 361/430 (83%), Gaps = 3/430 (0%) Query: 1 MTIISDVYAREVLDSRGNPTVEVDVYLESGAKGRAIVPSGASTGAHEAVELRDEDKNRYL 60 MTII+DVYAREVLDSRGNPTVEV+VYLESGA GRA+VPSGASTG +EAVELRD + R+L Sbjct: 1 MTIITDVYAREVLDSRGNPTVEVEVYLESGAMGRALVPSGASTGEYEAVELRDGGE-RFL 59 Query: 61 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMITLDGTHNKGKLGANXXXXXXXXXXX 120 GKGVLKAVENVNE+IAPE+IG DALDQ+GID+ MI LDGT NKGKLGAN Sbjct: 60 GKGVLKAVENVNEVIAPELIGFDALDQIGIDQHMIELDGTENKGKLGANAILGVSMAVAR 119 Query: 121 XXXXXXXXXXXXXXGGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR 180 GGFNAKQLPVPMMNI+NGG HADNNVD+QEFM++PVGA SFKEALR Sbjct: 120 AAANALDLPLYVYLGGFNAKQLPVPMMNIINGGEHADNNVDIQEFMIMPVGAESFKEALR 179 Query: 181 TGAEIFHALKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSIMEAIEKAGYKPGVDVFLG 240 TG EIFH+LK VLK KG NTAVGDEGGFAPN +SNE+AL +I+EAIE+AGY PG V L Sbjct: 180 TGTEIFHSLKKVLKSKGYNTAVGDEGGFAPNLSSNEEALQTIIEAIEQAGYTPGEQVKLA 239 Query: 241 MDVASTEFF--KDGKYHLEGEGKSFTPAEFVDLLTSWADKYPIITIEDGCSEDDWEGWKL 298 MDVAS+E + +DGKYHL GEGK + E V KYPII+IEDG E+DWEG K+ Sbjct: 240 MDVASSELYNKEDGKYHLSGEGKVLSSEEMVAFYEELVAKYPIISIEDGLDENDWEGHKM 299 Query: 299 LTEKLGNRIQLVGDDLFVTNTERLNKGIEEGIGNSILIKVNQIGTLTETFDAIEMAKRAG 358 LT++LG+++QLVGDDLFVTNT++L +GIE+G+GNSILIKVNQIGTLTETFDAIEMAKRAG Sbjct: 300 LTDRLGDKVQLVGDDLFVTNTKKLAQGIEQGVGNSILIKVNQIGTLTETFDAIEMAKRAG 359 Query: 359 YTAVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRIAKYNQLLRIEDELGELAQYN 418 YTAVISHRSGE+EDSTIADIAVATNAGQIKTGAPSRTDR+AKYNQLLRIEDELG LAQYN Sbjct: 360 YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDELGNLAQYN 419 Query: 419 GLKSFYNLKR 428 GL+SFYNLK+ Sbjct: 420 GLQSFYNLKK 429 >A4J8T7.1 A4J8T7_DESRM Enolase EC=4.2.1.11 Length = 428 Score = 644 bits (1660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/427 (73%), Positives = 358/427 (83%) Query: 1 MTIISDVYAREVLDSRGNPTVEVDVYLESGAKGRAIVPSGASTGAHEAVELRDEDKNRYL 60 MTIISDV+ARE+LDSRGNPT+E +V+LE G GRA VPSGASTGA+EAVELRD DK RYL Sbjct: 1 MTIISDVFAREILDSRGNPTLEAEVWLEDGTIGRAAVPSGASTGAYEAVELRDGDKGRYL 60 Query: 61 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMITLDGTHNKGKLGANXXXXXXXXXXX 120 GKGV++AVENVNEII PE+IGMDA DQ+GID+LMI LDGTHNKGKLGAN Sbjct: 61 GKGVVQAVENVNEIIGPELIGMDATDQIGIDRLMIELDGTHNKGKLGANAILGVSLAVAK 120 Query: 121 XXXXXXXXXXXXXXGGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR 180 GG NAK+LPVPMMNI+NGGAHADNNVD+QEFMVLPVGA SF E LR Sbjct: 121 AAANFLGLPLYQYIGGVNAKELPVPMMNILNGGAHADNNVDIQEFMVLPVGADSFSEGLR 180 Query: 181 TGAEIFHALKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSIMEAIEKAGYKPGVDVFLG 240 GAEIFH LKSVLK +GLNTAVGDEGGFAPN SNE+AL+ I+EAIEKAGYKPG DVFLG Sbjct: 181 MGAEIFHNLKSVLKARGLNTAVGDEGGFAPNLKSNEEALAVIIEAIEKAGYKPGQDVFLG 240 Query: 241 MDVASTEFFKDGKYHLEGEGKSFTPAEFVDLLTSWADKYPIITIEDGCSEDDWEGWKLLT 300 +DVA+TE +KDGKY LEGEG ++T E + DKYPIITIEDG SEDDWEGW LT Sbjct: 241 LDVAATEMYKDGKYVLEGEGVTYTSEEMIAFYQKLVDKYPIITIEDGLSEDDWEGWAKLT 300 Query: 301 EKLGNRIQLVGDDLFVTNTERLNKGIEEGIGNSILIKVNQIGTLTETFDAIEMAKRAGYT 360 + LG +IQLVGDDLFVTNTERL++GI++G+ NSILIKVNQIGTLTET DAIEMAKRAGYT Sbjct: 301 QALGKKIQLVGDDLFVTNTERLSQGIKKGVANSILIKVNQIGTLTETLDAIEMAKRAGYT 360 Query: 361 AVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRIAKYNQLLRIEDELGELAQYNGL 420 AV+SHRSGE++D+TIAD++VATNAGQIKTGAPSRTDR+AKYNQLLRIE+ELG++++Y G Sbjct: 361 AVVSHRSGETDDTTIADLSVATNAGQIKTGAPSRTDRVAKYNQLLRIEEELGDMSRYRGK 420 Query: 421 KSFYNLK 427 + FYNL+ Sbjct: 421 EVFYNLQ 427 >Q5WDK9.1 ENO_BACSK Enolase EC=4.2.1.11 Length = 429 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/430 (74%), Positives = 358/430 (83%), Gaps = 3/430 (0%) Query: 1 MTIISDVYAREVLDSRGNPTVEVDVYLESGAKGRAIVPSGASTGAHEAVELRDEDKNRYL 60 MTIISDVYAREVLDSRGNPTVEV+V+LESG GRA+VPSGASTG +EAVELRD +RY+ Sbjct: 1 MTIISDVYAREVLDSRGNPTVEVEVHLESGVMGRALVPSGASTGEYEAVELRD-GGDRYM 59 Query: 61 GKGVLKAVENVNEIIAPEVIGMDALDQLGIDKLMITLDGTHNKGKLGANXXXXXXXXXXX 120 GKGV KAV+NVNE IAPE+IG +ALDQ+GID+LMI LDGT NKG GAN Sbjct: 60 GKGVQKAVDNVNEKIAPELIGENALDQIGIDRLMIELDGTENKGNFGANAILGVSMAVAH 119 Query: 121 XXXXXXXXXXXXXXGGFNAKQLPVPMMNIVNGGAHADNNVDVQEFMVLPVGAPSFKEALR 180 GGFNAKQLPVPMMNI+NGG HADNNVD+QEFMV+PVGA SFKEALR Sbjct: 120 AAANALDIPLYVYLGGFNAKQLPVPMMNIINGGEHADNNVDIQEFMVMPVGAESFKEALR 179 Query: 181 TGAEIFHALKSVLKGKGLNTAVGDEGGFAPNFTSNEDALSSIMEAIEKAGYKPGVDVFLG 240 GAEIFH LKSVLK KG NTAVGDEGGFAPN +SNE+AL++I+EAIEKAGYKPG V L Sbjct: 180 MGAEIFHNLKSVLKAKGYNTAVGDEGGFAPNLSSNEEALATIIEAIEKAGYKPGEQVKLA 239 Query: 241 MDVASTEFF--KDGKYHLEGEGKSFTPAEFVDLLTSWADKYPIITIEDGCSEDDWEGWKL 298 MDVAS+E + +DGKYHL GEGK + E V KYPII+IEDG E+DWEG KL Sbjct: 240 MDVASSELYSKEDGKYHLAGEGKVLSSEEMVSFYEELVSKYPIISIEDGLDENDWEGHKL 299 Query: 299 LTEKLGNRIQLVGDDLFVTNTERLNKGIEEGIGNSILIKVNQIGTLTETFDAIEMAKRAG 358 LTE+LG+++QLVGDDLFVTNT++L +GIE+GIGNSILIKVNQIGTLTETFDAIEMAKRAG Sbjct: 300 LTERLGDKVQLVGDDLFVTNTKKLAEGIEKGIGNSILIKVNQIGTLTETFDAIEMAKRAG 359 Query: 359 YTAVISHRSGESEDSTIADIAVATNAGQIKTGAPSRTDRIAKYNQLLRIEDELGELAQYN 418 YTAVISHRSGE+ED+TIADIAVATNAGQIKTGAPSRTDR+AKYNQLLRIEDEL +LAQYN Sbjct: 360 YTAVISHRSGETEDATIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDELADLAQYN 419 Query: 419 GLKSFYNLKR 428 GLKSFYNL + Sbjct: 420 GLKSFYNLSK 429 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,464,318,146 Number of extensions: 105636505 Number of successful extensions: 218076 Number of sequences better than 1.0e-05: 2886 Number of HSP's gapped: 210275 Number of HSP's successfully gapped: 3524 Length of query: 428 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 289 Effective length of database: 1,751,175,118 Effective search space: 506089609102 Effective search space used: 506089609102 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)