BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0013.p (200 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3F3.1 B3K3F3_9BACI Short-chain dehydrogenase/reductase SDR 277 4e-73 A6M088.1 A6M088_CLOB8 Short-chain dehydrogenase/reductase SDR 251 4e-65 B1DD34.1 B1DD34_9BACL Short-chain dehydrogenase/reductase SDR 241 2e-62 B5JM23.1 B5JM23_9BACT KR domain superfamily 218 2e-55 B2J0E4.1 B2J0E4_NOSP7 Short-chain dehydrogenase/reductase SDR EC... 217 4e-55 >B3K3F3.1 B3K3F3_9BACI Short-chain dehydrogenase/reductase SDR Length = 247 Score = 277 bits (708), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 134/183 (73%), Positives = 154/183 (84%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 DIS+ A+IE+L+++T +G IDI+VNNAGIM I TEE FD+QFAINVKGTYF+ Sbjct: 65 DISQVAEIERLFRETIEAYGGIDILVNNAGIMTTKPIAAMTEEDFDQQFAINVKGTYFAI 124 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 QQAF H+ GGRIINFSTSV GQMFP YS+YAGTKGAVEQFTRQLAKEFG KGITINAVA Sbjct: 125 QQAFRHMNSGGRIINFSTSVAGQMFPTYSVYAGTKGAVEQFTRQLAKEFGPKGITINAVA 184 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGPV T+LF GKSE Q+ G+ K NAFGRLG+P+DIAGVV FLA+E+S WITGQTLRVNG Sbjct: 185 PGPVNTELFTVGKSEEQIAGIVKMNAFGRLGEPDDIAGVVLFLASEESKWITGQTLRVNG 244 Query: 198 GFI 200 GF+ Sbjct: 245 GFV 247 >A6M088.1 A6M088_CLOB8 Short-chain dehydrogenase/reductase SDR Length = 246 Score = 251 bits (640), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 109/183 (59%), Positives = 147/183 (80%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 D+S +E+L+ +T FG++DI++NNAG+++ L+ + TEE+FDK F INVKGTYF+C Sbjct: 64 DVSNIKDVEKLFSETITKFGRVDILINNAGVILYKLLSDVTEEEFDKLFNINVKGTYFAC 123 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 QQA H+E GRIINFSTSV G MFP YS+YA TKGAVEQ TRQLAKEFG K ITINAVA Sbjct: 124 QQAMKHMENNGRIINFSTSVVGSMFPTYSVYAATKGAVEQITRQLAKEFGPKKITINAVA 183 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGP+ T+LF GK++ Q+E +++ N+FGR+G+P+DIA + FL ++++ WITGQTLR+NG Sbjct: 184 PGPINTELFNVGKTDEQIEAIRQMNSFGRIGEPDDIANTIEFLVSDKAQWITGQTLRING 243 Query: 198 GFI 200 G++ Sbjct: 244 GYV 246 >B1DD34.1 B1DD34_9BACL Short-chain dehydrogenase/reductase SDR Length = 245 Score = 241 bits (616), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 114/182 (62%), Positives = 138/182 (75%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 DI K + +E L+ +T FG+IDI+VNNAGIM + I TEE FD+QF INVKGTYF+C Sbjct: 63 DIGKVSDVEGLFSETLERFGRIDILVNNAGIMDLAPIMNVTEEMFDRQFMINVKGTYFAC 122 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 QQA H+ GG IINFSTSV+G M P YS+YA TKGAVEQ TRQLAKEFG K I IN +A Sbjct: 123 QQAMKHMASGGTIINFSTSVSGAMLPTYSVYAATKGAVEQLTRQLAKEFGPKDIIINCIA 182 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PG V+T+LFL GKSE ++ ++ NAFGRLG+P DIA V L ++++ WITGQTLRVNG Sbjct: 183 PGQVSTELFLNGKSEELVDSFRRMNAFGRLGEPNDIANAVELLVSDKARWITGQTLRVNG 242 Query: 198 GF 199 GF Sbjct: 243 GF 244 >B5JM23.1 B5JM23_9BACT KR domain superfamily Length = 247 Score = 218 bits (556), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 97/182 (53%), Positives = 131/182 (71%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 D+++ +++ +L+ +T A +GK+D++VNNAGI+ N + TEE+FD+ FA+NVKGT+F+C Sbjct: 65 DVARVSEVTRLFDETIARYGKVDVLVNNAGILFNKPVAAVTEEEFDRIFAVNVKGTFFAC 124 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 QQA L GGRIIN S++ M P Y Y TKGAVEQ TR L+KE GS+GIT+NAV+ Sbjct: 125 QQAALRLSDGGRIINLSSTTTAVMLPTYGAYVATKGAVEQLTRTLSKELGSRGITVNAVS 184 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGP T+LF GKS ++ L AF RLG PE+IA ++FLA + SGWITGQ +R NG Sbjct: 185 PGPTDTELFKLGKSPEMIQRLANGAAFNRLGTPEEIAEAIAFLAGDASGWITGQNIRANG 244 Query: 198 GF 199 GF Sbjct: 245 GF 246 >B2J0E4.1 B2J0E4_NOSP7 Short-chain dehydrogenase/reductase SDR EC=1.1.1.100 Length = 246 Score = 217 bits (553), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 103/182 (56%), Positives = 134/182 (73%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 DISK I++L++ T FGK+DI+VNNAGI I + +EE FD FAINVKGT+F+C Sbjct: 64 DISKVPDIQRLFEQTLEHFGKVDILVNNAGIAFYKPITQVSEEDFDAIFAINVKGTFFAC 123 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 QQA HL +GGRIINFS+S M P YS Y GTKGAVEQ TR LAKE G+K I +N ++ Sbjct: 124 QQAAQHLSEGGRIINFSSSTTVMMLPTYSAYVGTKGAVEQITRVLAKELGAKAIAVNVIS 183 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGP T+LF EGK++ Q++ L + AFG+LG ++IA VV+FLA++++ WITGQ +RVNG Sbjct: 184 PGPTDTELFREGKTQEQIDRLAQMAAFGKLGDVQEIADVVAFLASDEARWITGQNIRVNG 243 Query: 198 GF 199 G Sbjct: 244 GI 245 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,029,645,365 Number of extensions: 39542486 Number of successful extensions: 180602 Number of sequences better than 1.0e-05: 23923 Number of HSP's gapped: 150284 Number of HSP's successfully gapped: 23957 Length of query: 200 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 71 Effective length of database: 1,845,385,268 Effective search space: 131022354028 Effective search space used: 131022354028 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)