BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0002.p (526 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5D9.1 B3K5D9_9BACI Glycoside hydrolase family 43 578 e-163 Q65MB7.1 Q65MB7_BACLD Putative uncharacterized protein 531 e-149 A3XP87.1 A3XP87_9FLAO Xylosidase/arabinosidase 531 e-149 C1SB16.1 C1SB16_9SPHI Beta-xylosidase 529 e-148 A4XGG5.1 A4XGG5_CALS8 Glycoside hydrolase, family 43 520 e-145 >B3K5D9.1 B3K5D9_9BACI Glycoside hydrolase family 43 Length = 528 Score = 578 bits (1490), Expect = e-163, Method: Compositional matrix adjust. Identities = 290/530 (54%), Positives = 362/530 (68%), Gaps = 19/530 (3%) Query: 1 MEVIMNTPAATDSGHWIPDQGNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLP 60 M+ + N P W+ DQG+GT++NP+++ADYSDPDVIRVGEDF+MTASSF H+PGLP Sbjct: 8 MQALQNKP-------WLSDQGDGTFVNPVLHADYSDPDVIRVGEDFYMTASSFGHLPGLP 60 Query: 61 ILHSRDLVNWRIVNHALPRLELPGYDKVQHGKGVWAPSLRYHEGQFWIFYATPDEGIFMT 120 ILHS+DL+NW+I+NH LPR++LPGYD QHG GVWAP+LR+H+G++W+FY PD GI M+ Sbjct: 61 ILHSKDLINWKIMNHVLPRMDLPGYDVPQHGNGVWAPALRFHDGKYWVFYGDPDVGILMS 120 Query: 121 KTDDPFGAWEPPHLLKAAKGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHILNLCRMSPDG 180 +DP G+W P HL++ KG IDPCPFWD+DGQAYLVHA+A SR G+KH+L +CRMS DG Sbjct: 121 TAEDPAGSWTPLHLVQEGKGLIDPCPFWDDDGQAYLVHAFAKSRCGIKHLLRMCRMSSDG 180 Query: 181 TTLLDDGTIIVDGTVKHPTLEGPKMYKRNGYYYIFAPAGGVPTGWQAVFRSKSIYGPYED 240 LLD+G I+ DGT +HPT+EGPK+YKRNGYYYIFAPAGGV TGWQ V RS S++GPYED Sbjct: 181 KQLLDEGQIVFDGTERHPTMEGPKLYKRNGYYYIFAPAGGVSTGWQTVLRSTSVFGPYED 240 Query: 241 KIVLQQGETPVNGPHQGGWVELASGESWFVHFQDKGAYGRITHLQPMEWRGDWPVMGRLA 300 KIVL QG+ PVNGPHQGGWVELASGESWF+HFQDKGAYGRITHLQPM W DWP +G Sbjct: 241 KIVLHQGDGPVNGPHQGGWVELASGESWFMHFQDKGAYGRITHLQPMRWVDDWPQIGIDT 300 Query: 301 PDREAGEPVERWKKPDTAAGEVCI--PQTSDDFCGAALGLQWQWQANPASNWHRLLPGGG 358 GEPV R+ KP + GE I P SD+F GA LGLQWQWQANP +W+ Sbjct: 301 NGDGIGEPVLRYPKP-SVLGEHMIQAPDDSDEFEGATLGLQWQWQANPNPDWYAFEKDSH 359 Query: 359 LRLNSAPLPGD--CRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCAERAGLVVLGHRAF 416 LRL S P+P +T+YD P LL+QK A VF A K+ ERAGL++ G Sbjct: 360 LRLFSFPVPEHRPSQTIYDAPHLLLQKFPAEVFHAEAKLDGTSLKPGERAGLIIFGRTFA 419 Query: 417 CIALSLDPAGDLWLAQYESGAADDPSRKDLGVKAAGILIQAESLYLRASFEEANGCRFSF 476 + + + G + E A +++ V + G + +R + CRF + Sbjct: 420 YVGVRMKTDG----SGMEIIQAKGNDKEENIVWSGG--WNDGKVTIRVTVHPGAICRFDY 473 Query: 477 SREGSDYIEFGSP-FRLEEGQWVGAKVGLFALSLTPGEDLGYADFERFTI 525 + Y+ G F E+G WVGAKVGLFA+ D GY D E F + Sbjct: 474 AAADGRYMPCGKKDFVAEKGHWVGAKVGLFAVCKEDHPDRGYVDVEWFRV 523 >Q65MB7.1 Q65MB7_BACLD Putative uncharacterized protein Length = 534 Score = 531 bits (1369), Expect = e-149, Method: Compositional matrix adjust. Identities = 271/516 (52%), Positives = 339/516 (65%), Gaps = 13/516 (2%) Query: 16 WIPDQGNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNH 75 W P+ G + NP+++ DYSDPDVIR G DFFM ASSF+ PG+PILHS DLVNW +++H Sbjct: 16 WNPNIDGGRFCNPVLFMDYSDPDVIRKGSDFFMAASSFSCFPGIPILHSTDLVNWSLISH 75 Query: 76 ALPRLELPGYDKVQHGKGVWAPSLRYHEGQFWIFYATPDEGIFMTKTDDPFGAWEPPHLL 135 L Y+ HGKG WAPS+RYH G+FW+++ATPDEGIFM+KT DPF WEP + Sbjct: 76 VFDELPFGEYEAPAHGKGAWAPSIRYHNGEFWVYFATPDEGIFMSKTSDPFKGWEPLIQV 135 Query: 136 KAAKGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHILNLCRMSPDGTTLLDDGTIIVDGTV 195 K KGWIDPCPFWD+DG AYL+HAYA+SR G+K LNLCRM DGT L DDG I+ DGT+ Sbjct: 136 KKTKGWIDPCPFWDDDGNAYLIHAYANSRIGIKSKLNLCRMKKDGTALEDDGEIVFDGTL 195 Query: 196 KHPTLEGPKMYKRNGYYYIFAPAGGVPTGWQAVFRSKSIYGPYEDKIVLQQGETPVNGPH 255 HPT+EGPK+YK++GYYYIFAPAGGV TGWQ V RSK IYGPYEDK+VL QG T +NGPH Sbjct: 196 NHPTIEGPKLYKKDGYYYIFAPAGGVKTGWQTVLRSKQIYGPYEDKVVLHQGNTVINGPH 255 Query: 256 QGGWVELASGESWFVHFQDKGAYGRITHLQPMEWRGDWPVMGRLAPDREAGEPVERWKKP 315 QGGWVEL SGESWF+HFQD+GA+GR+ LQP+ W WP+MG GEPV W+KP Sbjct: 256 QGGWVELDSGESWFIHFQDRGAFGRVVQLQPVRWFDGWPLMGEDINGDGIGEPVLHWEKP 315 Query: 316 DTAAGEVCIP--QTSDDFCGA-ALGLQWQWQANPASNWHRLLPG-GGLRLNSAPLPGDCR 371 T A EV +P +DDF + L L WQW+ANP W+ L G LRL S + + Sbjct: 316 KTGAKEVKLPFDHGNDDFSSSDRLALHWQWEANPKQEWYSLSASPGRLRLYSRGIAHPFK 375 Query: 372 TLYDTPQLLMQKLAAPVFTATVKITPRFSSCAERAGLVVLGHRAFCIALSLDPAGDLWLA 431 L P LLMQK + P FTATVK++ S AGL V+G + S G L+ Sbjct: 376 -LSRLPNLLMQKFSGPSFTATVKLSLNALSETMMAGLAVMGREYAYLGFSKSENG-YRLS 433 Query: 432 QYESGAADDPSRKDLGVKAAGILIQAESLYLRASFEEANGCRFSFSREGSDYIEFGSPFR 491 YE D ++D+ + G + AE +YLR + +E CRFS S + + + G+ F+ Sbjct: 434 VYEGRIEGD--QEDVKTEERG--VHAEMVYLRVTVDEQAVCRFSCSMDRRHFTQIGNSFQ 489 Query: 492 LEEGQWVGAKVGLFALSLTPGED--LGYADFERFTI 525 G W GAK G++ T GED G+ D E F++ Sbjct: 490 AVPGLWTGAKAGVYCAD-TGGEDGQDGWCDIEWFSV 524 >A3XP87.1 A3XP87_9FLAO Xylosidase/arabinosidase Length = 540 Score = 531 bits (1368), Expect = e-149, Method: Compositional matrix adjust. Identities = 261/517 (50%), Positives = 350/517 (67%), Gaps = 10/517 (1%) Query: 16 WIPDQGNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNH 75 W+ DQG+GTY NPII+ADYSDPDV+++G+D+FMT+SSF PGLPILHS+DLVNW +VN+ Sbjct: 27 WVADQGDGTYKNPIIHADYSDPDVVQLGDDYFMTSSSFNAAPGLPILHSKDLVNWELVNY 86 Query: 76 ALPR-LELPGYDKVQHGKGVWAPSLRYHEGQFWIFYATPDEGIFMTKTDDPFGAWEPPHL 134 ALP+ + + ++ QHG GVWAPS+R H + +I++ PD GI+M KTDDPFG WE P L Sbjct: 87 ALPKQVPVKHFNTPQHGNGVWAPSIRVHNNELYIYWGDPDFGIYMVKTDDPFGNWEEPVL 146 Query: 135 LKAAKGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHILNLCRMSPDGTTLLDDGTIIVDGT 194 + AKG IDP P WDEDG+AYLVHA+A SR+G+K IL + +M+ +GT +LD G + DG Sbjct: 147 MMEAKGAIDPSPLWDEDGKAYLVHAWAGSRAGVKSILTVHKMNAEGTKVLDYGRHVFDGH 206 Query: 195 VKHPTLEGPKMYKRNGYYYIFAPAGGVPTGWQAVFRSKSIYGPYEDKIVLQQGETPVNGP 254 HPT+EG K YKRNGYYYIFAPAGGV TGWQ V RSK+IYGPYE+K+VL+QG T +NGP Sbjct: 207 ENHPTVEGSKFYKRNGYYYIFAPAGGVSTGWQLVLRSKNIYGPYEEKVVLEQGATAINGP 266 Query: 255 HQGGWVELASGESWFVHFQDKGAYGRITHLQPMEWRGDWPVMGRLAPDREAGEPVERWKK 314 HQG WV +GE WF HFQD AYGRI HLQPM W+ DWPVMG+ GEPV + KK Sbjct: 267 HQGAWVTTDTGEDWFFHFQDVDAYGRIVHLQPMHWKNDWPVMGKDQNGNGIGEPVLQHKK 326 Query: 315 PDTAAG-EVCIPQTSDDFCGAALGLQWQWQANPASNWHRLLPGGG-LRLNSAPLPGDCRT 372 P+ + P+ +D+F +LGLQWQW ANP W LPG LRL S P + Sbjct: 327 PNVGEQYAIQTPRETDNFEQDSLGLQWQWNANPNVLWSAKLPGNDFLRLYSIAPPELMNS 386 Query: 373 LYDTPQLLMQKLAAPVFTATVKIT--PRFSSCAERAGLVVLGHRAFCIALSLDPAGDLWL 430 L+D P LL+QK AP F KI+ P + GL+++G + L+ + G +L Sbjct: 387 LWDVPNLLLQKFPAPEFNVATKISLIPGDGKEQRKTGLIIMGRDYATVTLT-EKEGVFYL 445 Query: 431 AQYESGAADDPSRKDLGVKAAGILIQAESLYLRASFEEANG-CRFSFSREGSDYIEFGSP 489 + E+ AD +++++ I +++ ++YL+ + + C+FS+S G ++ +FG+P Sbjct: 446 QRTEAIKADAGAKEEI---LEEITLKSNTVYLKVAVSAPDAMCQFSYSENGRNFKKFGAP 502 Query: 490 FRLEEGQWVGAKVGLFALSLTPGEDLGYADFERFTIS 526 F+ G+W+GAKVGLF++S GYAD E F I+ Sbjct: 503 FKARVGKWIGAKVGLFSISTPEASRGGYADVEYFRIT 539 >C1SB16.1 C1SB16_9SPHI Beta-xylosidase Length = 542 Score = 529 bits (1363), Expect = e-148, Method: Compositional matrix adjust. Identities = 263/514 (51%), Positives = 341/514 (66%), Gaps = 9/514 (1%) Query: 16 WIPDQGNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNH 75 W+ D GNG+Y NP++ ADYSDPDV R G +F+MTASSF PGLPIL S+DLVNW++V H Sbjct: 33 WVADLGNGSYKNPVLNADYSDPDVCRAGNEFYMTASSFNAAPGLPILQSKDLVNWQLVGH 92 Query: 76 ALPRLE-LPGYDKVQHGKGVWAPSLRYHEGQFWIFYATPDEGIFMTKTDDPFGAWEPPHL 134 AL R + KVQHG GVWAPS+RYH+G+F+I+Y PD GI+M + + G W P + Sbjct: 93 ALLRQPPFAHFSKVQHGNGVWAPSIRYHKGEFYIYYPDPDFGIYMIRAKNAAGPWSDPVM 152 Query: 135 LKAAKGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHILNLCRMSPDGTTLLDDGTIIVDGT 194 +KA KG IDPCP WD+DG+AYL+HA+A SR+G+K IL L RM+ GT + D+G ++ DG Sbjct: 153 VKAGKGLIDPCPLWDDDGKAYLIHAWAGSRAGIKSILTLNRMNETGTAVTDEGVLVFDGH 212 Query: 195 VKHPTLEGPKMYKRNGYYYIFAPAGGVPTGWQAVFRSKSIYGPYEDKIVLQQGETPVNGP 254 HPTLEGPK+YKRNGYYYIFAPAGGV TGWQ V RS++IYGPYE+KIV+ QG + VNGP Sbjct: 213 GAHPTLEGPKLYKRNGYYYIFAPAGGVSTGWQLVLRSRNIYGPYEEKIVMDQGRSVVNGP 272 Query: 255 HQGGWVELASGESWFVHFQDKGAYGRITHLQPMEWRGDWPVMGRLAPDREAGEPVERWKK 314 HQG WV++ SG SWF+HFQDKGAYGR+ HLQ M+W DWPV+G A GEPV ++K Sbjct: 273 HQGAWVDMPSGSSWFLHFQDKGAYGRVVHLQTMQWVKDWPVIGMDADGDGKGEPVMTYRK 332 Query: 315 PDTAAGEVCIPQTSDDFCGAALGLQWQWQANPASNWHRLLPGGG-LRLNSAPLPGDCRTL 373 P A V P SD+F A LGLQWQWQANP S W + P G LRL SA +P R Sbjct: 333 P-LAGMPVMTPPESDEFNSATLGLQWQWQANPVSTWMMMYPAKGVLRLFSAKMPDSSRNY 391 Query: 374 YDTPQLLMQKLAAPVFTATVK--ITPRFSSCAERAGLVVLGHRAFCIALSLDPAGDLWLA 431 + P LL+QK A FT T K TP + ER GL+++G I+L + L L Sbjct: 392 WQAPNLLLQKFPADSFTVTAKCTFTPNPALEGERTGLIIMGVDYAGISL-MKKDNQLQLL 450 Query: 432 QYESGAADDPSRKDLGVKAAGILIQAESLYLRASFEEANGCRFSFSREGSDYIEFGSPFR 491 AD + ++ V I + + R ++ C+FS+S +G+ +++ G PF Sbjct: 451 YASCLKADKGAAEEQEVLKT---IDGNTCWFRVKVDKGAVCQFSYSTDGTKFVDAGKPFT 507 Query: 492 LEEGQWVGAKVGLFALSLTPGEDLGYADFERFTI 525 + G+WVGAKVG+F + T D G+AD++ F + Sbjct: 508 AQPGRWVGAKVGIFCMRNTQINDSGFADYDWFRV 541 >A4XGG5.1 A4XGG5_CALS8 Glycoside hydrolase, family 43 Length = 528 Score = 520 bits (1340), Expect = e-145, Method: Compositional matrix adjust. Identities = 261/526 (49%), Positives = 338/526 (64%), Gaps = 26/526 (4%) Query: 16 WIPDQGNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNH 75 W P+ Y NPII+ADYSDPDVIRVG D+F+ ASSF +PGLPILHSRDL+NW+IVN+ Sbjct: 6 WDPNVDEERYKNPIIHADYSDPDVIRVGSDYFLVASSFNCVPGLPILHSRDLINWKIVNY 65 Query: 76 ALPRLELPGYDKVQHGKGVWAPSLRYHEGQFWIFYATPDEGIFMTKTDDPFGAWEPPHLL 135 A+ RL P YD Q GKG+WAP++RYHE +F+I+++ PD+GI++ TDDPFG W HL+ Sbjct: 66 AVERLPSPEYDTPQIGKGIWAPAIRYHEEKFYIYFSMPDDGIYVCYTDDPFGKWSDIHLV 125 Query: 136 KAAKGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHILNLCRMSPDGTTLLDDGTIIVDGTV 195 KAAKGWIDPCPFWDEDG AYLV+A+A SR G L + R+S DG +LD+G I+ DG Sbjct: 126 KAAKGWIDPCPFWDEDGNAYLVNAFAKSRIGFNSKLCVSRLSADGLRVLDEGKIVFDGAK 185 Query: 196 KHPTLEGPKMYKRNGYYYIFAPAGGVPTGWQAVFRSKSIYGPYEDKIVLQQGETPVNGPH 255 PT+EGPK+YKRNGYYYIFAPAGGV GWQ V RSK IYGPYE+KIVL QG+T +NGPH Sbjct: 186 TQPTIEGPKLYKRNGYYYIFAPAGGVKFGWQTVLRSKDIYGPYEEKIVLHQGKTKINGPH 245 Query: 256 QGGWVELASGESWFVHFQDKGAYGRITHLQPMEWRGDWPVMGRLAPDREAGEPVERWKKP 315 QG WVE GE WFVHFQD+G YGR+ HLQPM W +WPV+G GEPVE ++KP Sbjct: 246 QGAWVETEKGEHWFVHFQDRGPYGRVVHLQPMRWEDNWPVIGVDIDGDGIGEPVEEYRKP 305 Query: 316 DTAAGEV--CIPQTSDDFCGAALGLQWQWQANPASNWH---------RLLPGGGLRLNSA 364 +T + SD F + LQWQW ANP NW+ RL G L N+ Sbjct: 306 NTNYKNEYDFYLECSDHFDKPQISLQWQWHANPQDNWYSLEKRKSYLRLYAVGNLTNNNK 365 Query: 365 PLPGDCRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCAERAGLVVLGHRAFCIALSLDP 424 ++++ P LL+QK+ AP F A VK+ F+ AG+++ G R I + Sbjct: 366 -----VKSIFFMPNLLLQKIPAPEFIAIVKLEVNFNKEGSYAGIIMSGSRYSSIGIKRGQ 420 Query: 425 AGDLWLAQYESGAADDPSRKDLGVKAAGILIQAESLYLRASFEEANGCRFSFSREGSDYI 484 L L Q +D + A + I+ ++L+ S +E C+FSFS +G + Sbjct: 421 K-KLSLVQVLGDVKEDVIENTV----AKMPIETSQVFLKISVKEGAVCKFSFSFDGKKFT 475 Query: 485 EFGSPFRLEEGQWVGAKVGLFALSLTPGED----LGYADFERFTIS 526 +FG+ F +E WVG+K+GLF ++ T G++ YADF+ F I Sbjct: 476 DFGTEFIPQEAIWVGSKIGLFCIN-TSGKNGVIQSDYADFDYFKIE 520 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.458 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,850,432,767 Number of extensions: 186633495 Number of successful extensions: 303108 Number of sequences better than 1.0e-05: 595 Number of HSP's gapped: 301197 Number of HSP's successfully gapped: 605 Length of query: 526 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 385 Effective length of database: 1,732,333,088 Effective search space: 666948238880 Effective search space used: 666948238880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)