BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0006.p (255 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA55.1 B3KA55_9BACI Acyl-ACP thioesterase 229 2e-58 B9M0X0.1 B9M0X0_GEOSF Acyl-ACP thioesterase 103 1e-20 C1QHS4.1 C1QHS4_CAPOC Acyl-ACP thioesterase 100 1e-19 B8I625.1 B8I625_CLOCE Acyl-ACP thioesterase 95 5e-18 Q74KZ2.1 Q74KZ2_LACJO Putative uncharacterized protein 94 9e-18 >B3KA55.1 B3KA55_9BACI Acyl-ACP thioesterase Length = 247 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 112/237 (47%), Positives = 159/237 (67%), Gaps = 2/237 (0%) Query: 16 WTEQYSVHASDTDYRSQGKLSFVLDMMQRAADSAVGGSGVSLEQMLEAGIGWMLITLELE 75 WTE+Y++ + D D++ + S +L ++QRAAD + G+S E+M+E G+GWMLITLELE Sbjct: 8 WTEEYTIQSVDADFKGDCRWSSLLSILQRAADRHIEALGISREEMIERGMGWMLITLELE 67 Query: 76 FKRMPRPNDLLTIHTWSKGTKGALWQRDYRIFDSSQIEIVTARSVWALVDIAKRKILRPS 135 +RMPR + + + TWS+G+KGALW RDYRI + + RSVWALVDI KRKILRPS Sbjct: 68 MRRMPRDMENVYVDTWSRGSKGALWHRDYRIKNGDGELLGEGRSVWALVDIHKRKILRPS 127 Query: 136 ALPVKVEHYTGDSVGSMPQKVTIPPDLSLVEAYRYQVRYSGLDSNSHLNNARYGELCTDT 195 P +V ++VG +P K +P + L +AY Y VRYSG+D+N HLNNARY +LC D Sbjct: 128 MFPYEVP-IGQETVGELPSKAVLPEGVQLDDAYTYSVRYSGIDTNGHLNNARYADLCFDV 186 Query: 196 LSQKEWEQLELASFRISYLQEAIFGDEMVILRTPITDQGMFVRGQSGE-TVFFEACL 251 L ++E + + F+I+YL EA D M+I R+ + ++V+G S + T FFEA + Sbjct: 187 LDEQELREGLVTGFKITYLNEARLKDTMLIKRSAEENNRVYVQGTSPDGTNFFEAAI 243 >B9M0X0.1 B9M0X0_GEOSF Acyl-ACP thioesterase Length = 257 Score = 103 bits (257), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 69/221 (31%), Positives = 105/221 (47%), Gaps = 14/221 (6%) Query: 8 MDNLTHLIWTEQYSVHASDTDYRSQGKLSFVLDMMQRAADSAVGGSGVSLEQMLEAGIGW 67 MDN ++ + V + D + K + + + MQ AA S V G+S+ + + + W Sbjct: 1 MDN----VFEHKLRVRYYEVDCKDSIKPAAIFNFMQDAAASHVRQLGLSVADLGKKNLTW 56 Query: 68 MLITLELEFKRMPRPNDLLTIHTWSKGTKGALWQRDYRIFDSSQIEIVTARSVWALVDIA 127 ++ L PR ND +TIHTW G R+Y I D + I A S WA V+I Sbjct: 57 VISRFHLRLLHYPRGNDRITIHTWPSTKDGLFSCREYTIRDENGKVIALATSSWAAVNIT 116 Query: 128 KRKILRPS----ALPVKVEHYTGDSVGSMPQKVTIPPDLSLVEAYRYQVRYSGLDSNSHL 183 R+ +R P+K E D S+P+ I + R+QVR S LD N H+ Sbjct: 117 TRRPVRLKDHLRDFPLKSERAIEDDFPSLPRPGRIDGE------QRFQVRRSDLDINRHV 170 Query: 184 NNARYGELCTDTLSQKEWEQLELASFRISYLQEAIFGDEMV 224 NNA Y + +T+ E+ LA +S+ EA++GD +V Sbjct: 171 NNAVYVDWALETVPLSIAEKYRLAEIEVSFRGEALYGDNVV 211 >C1QHS4.1 C1QHS4_CAPOC Acyl-ACP thioesterase Length = 253 Score = 100 bits (248), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 62/212 (29%), Positives = 107/212 (50%), Gaps = 9/212 (4%) Query: 15 IWTEQYSVHASDTDYRSQGKLSFVLDMMQRAADSAVGGSGVSLEQMLEAGIGWMLITLEL 74 I+TE Y V ++ + +Q L VL M+Q A G E++++ G W LI +L Sbjct: 6 IFTETYKVRSTQVNLNNQLGLYGVLGMLQDIAAEHASLLGFGYEELVKKGFFWALIQQKL 65 Query: 75 EFKRMPRPNDLLTIHTWSKGTKGALWQRDYRIFDSSQIEIVTARSVWALVDIAKRKILRP 134 + P+ N+ +T+ TWS KG R++ +F + Q +I S W +DI R RP Sbjct: 66 KMHYWPQWNERITVKTWSLPVKGVYAFREFELFCNDQ-KIGECASTWITLDIQTR---RP 121 Query: 135 SALPVKVEHYTGDSVGSMP---QKVTIPPDLSLVEAYRYQVRYSGLDSNSHLNNARYGEL 191 L + E ++ + G + ++ +P + V ++ VR S LD NSH+NN +Y + Sbjct: 122 IELTDRQEIFSPRTEGGLSFHTDRINLPEGMYQVNDFK--VRVSDLDVNSHVNNVKYTQW 179 Query: 192 CTDTLSQKEWEQLELASFRISYLQEAIFGDEM 223 D ++++ + + + I++L E GDEM Sbjct: 180 ALDMMTERNHREFVIKEYDINFLSETFMGDEM 211 >B8I625.1 B8I625_CLOCE Acyl-ACP thioesterase Length = 252 Score = 95.1 bits (235), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 56/232 (24%), Positives = 119/232 (51%), Gaps = 9/232 (3%) Query: 15 IWTEQYSVHASDTDYRSQGKLSFVLDMMQRAADSAVGGSGVSLEQML-EAGIGWMLITLE 73 I+ + Y V D D+ + KLS++ + Q A + V + ++ + G W++ + Sbjct: 6 IYKKNYHVDYGDADFYKRLKLSYLFNYFQNIAGLHSENTNVGIRKLQNDYGAAWVMTRML 65 Query: 74 LEFKRMPRPNDLLTIHTWSKGTKGALWQRDYRIFDSSQIEIVTARSVWALVDIAKRKILR 133 ++ RMP N+ ++I TW K + R++ + D + +A S W ++D+ KR+++R Sbjct: 66 MDINRMPECNEEISIETWPVEPKKKMIDRNFIVRDMDGSILASAISTWVILDMEKREMVR 125 Query: 134 -PSALPVKVEHYTGDSVGSMPQKV-TIPPDLSLVEAYRYQVRYSGLDSNSHLNNARYGEL 191 S +P + + ++ +K + P L AY+ V +S +D N H+NNA+Y + Sbjct: 126 IDSVIPPQYPEFLKSK--AIDRKFDKLKPSGQLQPAYKKLVGFSDIDINGHVNNAKYIDY 183 Query: 192 CTDTLSQKEWEQLELASFRISYLQEAIFGDEMVILRTPI----TDQGMFVRG 239 D + ++ + + S +I+Y+ EA+ GD + + + + +D+ +++ G Sbjct: 184 ILDCFTVEKHGEYRVKSIQINYVNEAVAGDIISLYKDTVDMDGSDKAVYITG 235 >Q74KZ2.1 Q74KZ2_LACJO Putative uncharacterized protein Length = 244 Score = 94.4 bits (233), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 53/210 (25%), Positives = 103/210 (49%), Gaps = 2/210 (0%) Query: 15 IWTEQYSVHASDTDYRSQGKLSFVLDMMQRAADSAVGGSGVSLEQMLEAGIGWMLITLEL 74 I+ E++ V D D + +L +++ + ++ + G + +L+ +GW+++ L Sbjct: 4 IFKEEHQVSYGDCDETGKIQLPHLIEHFMQVSNDQLTAGGAGIHDLLKQNLGWVVVEYHL 63 Query: 75 EFKRMPRPNDLLTIHTWSKGTKGALWQRDYRIFDSSQIEIVTARSVWALVDIAKRKILRP 134 + R+P + +T+ T G RD+ I DS+ +IV +S W ++D+ RKI Sbjct: 64 DIDRLPEAGEKITVTTNGSGYNRFFEYRDFGIIDSTNKKIVGVKSQWVILDLKNRKI--T 121 Query: 135 SALPVKVEHYTGDSVGSMPQKVTIPPDLSLVEAYRYQVRYSGLDSNSHLNNARYGELCTD 194 A ++ + + MP+ + P + +Y VRY LD+N HL N+ Y + D Sbjct: 122 EADDQMMQKFGNPYLKHMPRFKRLRPLKEYRSSKKYTVRYYDLDTNHHLTNSIYFDWMID 181 Query: 195 TLSQKEWEQLELASFRISYLQEAIFGDEMV 224 TL ++ + S IS+ +E +GD+ + Sbjct: 182 TLPREFLNSHTVKSIDISFKKEVQYGDQAL 211 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,549,795,204 Number of extensions: 60538838 Number of successful extensions: 123001 Number of sequences better than 1.0e-05: 233 Number of HSP's gapped: 122965 Number of HSP's successfully gapped: 233 Length of query: 255 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 122 Effective length of database: 1,807,701,208 Effective search space: 220539547376 Effective search space used: 220539547376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)