BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0008.p (441 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8T8.1 B3K8T8_9BACI Gluconate transporter 569 e-160 C2VBE3.1 C2VBE3_BACCE Gluconate permease 429 e-118 C2UUX2.1 C2UUX2_BACCE Gluconate permease 429 e-118 Q738U0.1 Q738U0_BACC1 Gluconate transporter, permease protein 428 e-118 C2TWZ4.1 C2TWZ4_BACCE Gluconate permease 427 e-118 >B3K8T8.1 B3K8T8_9BACI Gluconate transporter Length = 453 Score = 569 bits (1467), Expect = e-160, Method: Compositional matrix adjust. Identities = 289/441 (65%), Positives = 333/441 (75%) Query: 1 LLIWTLLVIVFLVMLIARFKWNPFVTXXXXXXXXXXXXXMEYPSLIKSXXXXXXXXXXXX 60 L+IWTLL IVFL++LI+++KWNPFVT M+ +I S Sbjct: 13 LMIWTLLTIVFLIVLISKYKWNPFVTLLISALILGLLTGMQPADVITSITGGLGGTLGTI 72 Query: 61 XXXXXXXXMLGKMMAESGGAERIADTLVDRFGEKRVHYAMMLVGFVVGIPVFFEVGLILL 120 MLGKMMAESGGAE+IA TLVDRFGEKRVH+AMM+VGF+VGIPVFFEVG+ILL Sbjct: 73 AIVIALGTMLGKMMAESGGAEQIATTLVDRFGEKRVHWAMMIVGFIVGIPVFFEVGVILL 132 Query: 121 IPVVFMVARKTKMPLLQIGIPILAGLSTVHGLVPPHPAPMIAIGAYGANLGKTILYSMIV 180 IP+VF VARKTKM LL IGIPILAGLSTVHGLVPPHPAPMIAI AY A+LG TILYS +V Sbjct: 133 IPIVFTVARKTKMSLLHIGIPILAGLSTVHGLVPPHPAPMIAIEAYDADLGMTILYSFLV 192 Query: 181 GLPTAIIAGPLFGKFIGKRIRVEPPRELAEQFAAKSVRELPGFGXXXXXXXXXXXXXXXG 240 G+P AI+AGP+FGKFIGKRIR EPP ELAEQF++K+ R LPGFG G Sbjct: 193 GIPAAILAGPVFGKFIGKRIRTEPPEELAEQFSSKAERGLPGFGITLFTILLPVILMLIG 252 Query: 241 SVADIIDPEASSAFTPFAKFIGHEIIALLIAAVFSFFSLGFARGFKKEEISRFTSECLAP 300 S ADIIDPE++S + F KFIGHEIIALLIAAVFSFFSLGFARGF K ++SRFTSECLAP Sbjct: 253 SAADIIDPESTSFISVFCKFIGHEIIALLIAAVFSFFSLGFARGFNKHDLSRFTSECLAP 312 Query: 301 TASXXXXXXXXXAFKQVLIDSGVGGAIAALATQTHVNXXXXXXXXXXXXRVATGSATVAM 360 A+ AFKQVLI+SGVG AIA +AT ++N R+ATGSATVAM Sbjct: 313 IATIILIIGAGGAFKQVLINSGVGNAIAEIATGANINVILFAWLVAALIRIATGSATVAM 372 Query: 361 TTAAGIIAPVLAVTPGVNIELAVLATGAGSLVLSHVNDAGFWMIKEFFGMSVVQTLKSWT 420 TTAAGI+APVLA+TPG N+EL VLATGAGS+VLSHVNDAGFWM+KEFF M+V QTLKSWT Sbjct: 373 TTAAGIVAPVLAMTPGANVELVVLATGAGSIVLSHVNDAGFWMVKEFFNMTVAQTLKSWT 432 Query: 421 VMETILSVVGLTFIMILSIFV 441 V+ETILSV GL ++L++ V Sbjct: 433 VLETILSVTGLVLALVLNVIV 453 >C2VBE3.1 C2VBE3_BACCE Gluconate permease Length = 449 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 224/430 (52%), Positives = 291/430 (67%), Gaps = 9/430 (2%) Query: 20 KWNPFVTXXXXXXXXXXXXXM-------EYPSLIKSXXXXXXXXXXXXXXXXXXXXMLGK 72 KW+PFV M ++P +I S MLGK Sbjct: 21 KWHPFVALILTAIGVGLAMGMPLVATSPQHPGIIDSIKSGLGSTLGFLAIVLALGTMLGK 80 Query: 73 MMAESGGAERIADTLVDRFGEKRVHYAMMLVGFVVGIPVFFEVGLILLIPVVFMVARKTK 132 MMAESGGAERIA+TL+DRFG+KRVH+AMM V F+VGIPVFF+VG +LLIP+VF +A +T Sbjct: 81 MMAESGGAERIANTLIDRFGKKRVHWAMMFVAFLVGIPVFFQVGFVLLIPLVFTIAIETG 140 Query: 133 MPLLQIGIPILAGLSTVHGLVPPHPAPMIAIGAYGANLGKTILYSMIVGLPTAIIAGPLF 192 + L+ IGIP++AGLS VHGLVPPHPA M A+G + A++GKTILY++IVGLPTAII+GPL+ Sbjct: 141 VSLITIGIPLVAGLSVVHGLVPPHPAAMAAVGIFKADVGKTILYALIVGLPTAIISGPLY 200 Query: 193 GKFIGKRIRVEPPRELAEQFAAKSVR-ELPGFGXXXXXXXXXXXXXXXGSVADIIDPEAS 251 GK+IG RI E P ++AEQF + + ELP FG S+A++ + Sbjct: 201 GKWIGARIHKEVPLDIAEQFIERDKKKELPSFGNTLFTILLPVFLMLGASIAEV-ALHKT 259 Query: 252 SAFTPFAKFIGHEIIALLIAAVFSFFSLGFARGFKKEEISRFTSECLAPTASXXXXXXXX 311 S FIG I+ALLIA ++SFFSLG+A+GF K+++ +FT++CL P A+ Sbjct: 260 SQLAQVLHFIGDPIVALLIATIYSFFSLGYAKGFSKDKVLQFTNDCLGPIANILLVIGAG 319 Query: 312 XAFKQVLIDSGVGGAIAALATQTHVNXXXXXXXXXXXXRVATGSATVAMTTAAGIIAPVL 371 AF +VL+DSG+G IA +A ++H++ RVATGSATV+M TAAGI+AP+ Sbjct: 320 GAFNKVLLDSGIGTTIAEMAKESHISPILLGWGIAALIRVATGSATVSMMTAAGIVAPIA 379 Query: 372 AVTPGVNIELAVLATGAGSLVLSHVNDAGFWMIKEFFGMSVVQTLKSWTVMETILSVVGL 431 A TPGVN+EL LATGAGSL+LSHVND+GFWMIKE+FGM+V +TL +WTVMETILSVV L Sbjct: 380 ASTPGVNVELLALATGAGSLILSHVNDSGFWMIKEYFGMTVKETLLTWTVMETILSVVAL 439 Query: 432 TFIMILSIFV 441 I +L+IFV Sbjct: 440 GLISLLNIFV 449 >C2UUX2.1 C2UUX2_BACCE Gluconate permease Length = 452 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 224/430 (52%), Positives = 290/430 (67%), Gaps = 9/430 (2%) Query: 20 KWNPFVTXXXXXXXXXXXXXM-------EYPSLIKSXXXXXXXXXXXXXXXXXXXXMLGK 72 KW+PFV M ++P +I S MLGK Sbjct: 24 KWHPFVALILTAIGVGLAMGMPLVATSPQHPGIIDSIKSGLGSTLGFLAIVLALGTMLGK 83 Query: 73 MMAESGGAERIADTLVDRFGEKRVHYAMMLVGFVVGIPVFFEVGLILLIPVVFMVARKTK 132 MMAESGGAERIA+TL+DRFG+KRVH+AMM V F+VGIPVFF+VG +LLIP+VF +A +T Sbjct: 84 MMAESGGAERIANTLIDRFGKKRVHWAMMFVAFLVGIPVFFQVGFVLLIPLVFTIAIETG 143 Query: 133 MPLLQIGIPILAGLSTVHGLVPPHPAPMIAIGAYGANLGKTILYSMIVGLPTAIIAGPLF 192 + L+ IGIP++AGLS VHGLVPPHPA M A+G + A++GKTILY++IVGLPTAII+GPL+ Sbjct: 144 VSLITIGIPLVAGLSVVHGLVPPHPAAMAAVGIFKADVGKTILYALIVGLPTAIISGPLY 203 Query: 193 GKFIGKRIRVEPPRELAEQFAAKSVR-ELPGFGXXXXXXXXXXXXXXXGSVADIIDPEAS 251 GK+IG RI E P ++AEQF + + ELP FG S+A++ + Sbjct: 204 GKWIGARIHKEVPLDIAEQFIERDKKKELPSFGNTLFTILLPVFLMLGASIAEV-ALHKT 262 Query: 252 SAFTPFAKFIGHEIIALLIAAVFSFFSLGFARGFKKEEISRFTSECLAPTASXXXXXXXX 311 S FIG I+ALLIA ++SFFSLG+A+GF K+++ +FT++CL P A+ Sbjct: 263 SQLAQVLHFIGDPIVALLIATIYSFFSLGYAKGFSKDKVLQFTNDCLGPIANILLVIGAG 322 Query: 312 XAFKQVLIDSGVGGAIAALATQTHVNXXXXXXXXXXXXRVATGSATVAMTTAAGIIAPVL 371 AF +VL+DSG+G IA +A ++H++ RVATGSATV+M TAAGI+AP+ Sbjct: 323 GAFNKVLLDSGIGTTIAEMAKESHISPILLGWGIASLIRVATGSATVSMMTAAGIVAPIA 382 Query: 372 AVTPGVNIELAVLATGAGSLVLSHVNDAGFWMIKEFFGMSVVQTLKSWTVMETILSVVGL 431 A TPGVN+EL LATGAGSL+LSHVND+GFWMIKE+FGM+V +TL +WT METILSVV L Sbjct: 383 ASTPGVNVELLALATGAGSLILSHVNDSGFWMIKEYFGMTVKETLLTWTAMETILSVVAL 442 Query: 432 TFIMILSIFV 441 I IL+IFV Sbjct: 443 GLISILNIFV 452 >Q738U0.1 Q738U0_BACC1 Gluconate transporter, permease protein Length = 449 Score = 428 bits (1100), Expect = e-118, Method: Compositional matrix adjust. Identities = 224/430 (52%), Positives = 290/430 (67%), Gaps = 9/430 (2%) Query: 20 KWNPFVTXXXXXXXXXXXXXM-------EYPSLIKSXXXXXXXXXXXXXXXXXXXXMLGK 72 KW+PFV M + P +I S MLGK Sbjct: 21 KWHPFVALILTAIGVGLAMGMPLIGTSPKDPGIIDSIKLGLGNTLGFLAIVLALGTMLGK 80 Query: 73 MMAESGGAERIADTLVDRFGEKRVHYAMMLVGFVVGIPVFFEVGLILLIPVVFMVARKTK 132 MMAESGGAERIA+TL+DRFG+KRVH+AMM V F+VGIPVFF+VG +LLIP+VF +A +T Sbjct: 81 MMAESGGAERIANTLIDRFGKKRVHWAMMFVAFLVGIPVFFQVGFVLLIPLVFTIALETG 140 Query: 133 MPLLQIGIPILAGLSTVHGLVPPHPAPMIAIGAYGANLGKTILYSMIVGLPTAIIAGPLF 192 + L+ IGIP++AGLS VHGLVPPHPA M A+G + A++GKTILY++IVGLPTAII+GPL+ Sbjct: 141 VSLITIGIPLVAGLSVVHGLVPPHPAAMAAVGIFKADVGKTILYALIVGLPTAIISGPLY 200 Query: 193 GKFIGKRIRVEPPRELAEQFAAK-SVRELPGFGXXXXXXXXXXXXXXXGSVADIIDPEAS 251 GK+IG RI E P E+AEQF K + +ELP FG S+A+ + + Sbjct: 201 GKWIGARIHKEVPLEIAEQFVEKDNKKELPSFGNTLFTILLPVFLMLGASIAE-VALNKT 259 Query: 252 SAFTPFAKFIGHEIIALLIAAVFSFFSLGFARGFKKEEISRFTSECLAPTASXXXXXXXX 311 S FIG I+ALLIA ++SFFSLG+A+GF K+++ +FT++CL P A+ Sbjct: 260 SQLAQVLHFIGDPIVALLIATIYSFFSLGYAKGFSKDKVLQFTNDCLGPIANILLVIGAG 319 Query: 312 XAFKQVLIDSGVGGAIAALATQTHVNXXXXXXXXXXXXRVATGSATVAMTTAAGIIAPVL 371 AF +VL+DSG+G IA +A ++H++ R+ATGSATV+M TAAGI+AP+ Sbjct: 320 GAFNKVLLDSGIGTTIADMAKESHISPILLGWGIAALIRIATGSATVSMMTAAGIVAPIA 379 Query: 372 AVTPGVNIELAVLATGAGSLVLSHVNDAGFWMIKEFFGMSVVQTLKSWTVMETILSVVGL 431 A TPGVN+EL LATGAGSL+LSHVND+GFWMIKE+FGM+V +TL +WT METILSVV L Sbjct: 380 ASTPGVNVELLALATGAGSLILSHVNDSGFWMIKEYFGMTVKETLLTWTAMETILSVVAL 439 Query: 432 TFIMILSIFV 441 I +L+IFV Sbjct: 440 GLISLLNIFV 449 >C2TWZ4.1 C2TWZ4_BACCE Gluconate permease Length = 449 Score = 427 bits (1099), Expect = e-118, Method: Compositional matrix adjust. Identities = 223/430 (51%), Positives = 290/430 (67%), Gaps = 9/430 (2%) Query: 20 KWNPFVTXXXXXXXXXXXXXM-------EYPSLIKSXXXXXXXXXXXXXXXXXXXXMLGK 72 KW+PFV M ++P +I S MLGK Sbjct: 21 KWHPFVALILTAIGVGLAMGMPLVATSPQHPGIIDSIKSGLGSTLGFLAIVLALGTMLGK 80 Query: 73 MMAESGGAERIADTLVDRFGEKRVHYAMMLVGFVVGIPVFFEVGLILLIPVVFMVARKTK 132 MMAESGGAERIA+TL+DRFG+KRVH+AMM V F+VGIPVFF+VG +LLIP+VF +A +T Sbjct: 81 MMAESGGAERIANTLIDRFGKKRVHWAMMFVAFLVGIPVFFQVGFVLLIPLVFTIAIETG 140 Query: 133 MPLLQIGIPILAGLSTVHGLVPPHPAPMIAIGAYGANLGKTILYSMIVGLPTAIIAGPLF 192 + L+ IGIP++AGLS VHGLVPPHPA M A+G + A++GKTILY++IVGLPTAII+GPL+ Sbjct: 141 VSLITIGIPLVAGLSVVHGLVPPHPAAMAAVGIFKADVGKTILYALIVGLPTAIISGPLY 200 Query: 193 GKFIGKRIRVEPPRELAEQFAAKSVR-ELPGFGXXXXXXXXXXXXXXXGSVADIIDPEAS 251 GK+IG RI E P ++AEQF + + ELP FG S+A++ + Sbjct: 201 GKWIGARIHKEVPLDIAEQFIERDKKKELPSFGNTLFTILLPVFLMLGASIAEV-ALHKT 259 Query: 252 SAFTPFAKFIGHEIIALLIAAVFSFFSLGFARGFKKEEISRFTSECLAPTASXXXXXXXX 311 S FIG I+ALLIA ++SFFSLG+A+GF K+++ +FT++CL P A+ Sbjct: 260 SQLAQVLHFIGDPIVALLIATIYSFFSLGYAKGFSKDKVLQFTNDCLGPIANILLVIGAG 319 Query: 312 XAFKQVLIDSGVGGAIAALATQTHVNXXXXXXXXXXXXRVATGSATVAMTTAAGIIAPVL 371 AF +VL+DSG+G IA +A ++H++ RVATGSATV+M TAAGI+AP+ Sbjct: 320 GAFNKVLLDSGIGTTIAEMAKESHISPILLGWGIAALIRVATGSATVSMMTAAGIVAPIA 379 Query: 372 AVTPGVNIELAVLATGAGSLVLSHVNDAGFWMIKEFFGMSVVQTLKSWTVMETILSVVGL 431 A TPGVN+EL LATGAGSL+LSHVND+GFWMIKE+FGM+V +TL +WT METILSVV L Sbjct: 380 ASTPGVNVELLALATGAGSLILSHVNDSGFWMIKEYFGMTVKETLLTWTAMETILSVVAL 439 Query: 432 TFIMILSIFV 441 I +L+IFV Sbjct: 440 GLISLLNIFV 449 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,048,321,063 Number of extensions: 74366598 Number of successful extensions: 367332 Number of sequences better than 1.0e-05: 1344 Number of HSP's gapped: 363763 Number of HSP's successfully gapped: 1423 Length of query: 441 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 302 Effective length of database: 1,751,175,118 Effective search space: 528854885636 Effective search space used: 528854885636 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)