BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0004.p (217 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKW7.1 B1DKW7_9BACL HAD-superfamily hydrolase, subfamily IA, v... 187 5e-46 Q65EF3.1 Q65EF3_BACLD P-Ser-HPr phosphatase 170 8e-41 C2WEB3.1 C2WEB3_BACCE Pyrophosphatase ppaX 169 2e-40 Q8ENK3.1 PPAX_OCEIH Pyrophosphatase ppaX EC=3.6.1.1 169 2e-40 C3BSE3.1 C3BSE3_9BACI Pyrophosphatase ppaX 168 4e-40 >B1DKW7.1 B1DKW7_9BACL HAD-superfamily hydrolase, subfamily IA, variant 3 Length = 217 Score = 187 bits (475), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 96/209 (45%), Positives = 131/209 (62%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I+ +LFDLDGTI++TNELII SFM +LK RE IIP MG TL Q F+GL D Sbjct: 5 IKTMLFDLDGTIMDTNELIIRSFMESLKGLVPEDFGREHIIPSMGLTLQAQFQNFTGLTD 64 Query: 62 TSALELSYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMFDL 121 L +YR+ N HDEL+ FP+ NE+M+ L +GI++G+VTTKIR +T + ++ L Sbjct: 65 VEHLIKAYRTVNTKLHDELLRPFPYTNEVMKNLHEQGIQIGVVTTKIRMSTERGMKFCGL 124 Query: 122 LKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXXXX 181 Y++ VTV+DV PKPH EPVL A+ L DP T+M+GDS VD+ S Sbjct: 125 YDYVDAFVTVDDVANPKPHPEPVLMALEMLNADPATTIMIGDSTVDMISAKEAGVIPVGV 184 Query: 182 XWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 WSLKG ++L++ YII DM++++ Sbjct: 185 SWSLKGGELLKENGAQYIIDDMRELYAFA 213 >Q65EF3.1 Q65EF3_BACLD P-Ser-HPr phosphatase Length = 215 Score = 170 bits (430), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 88/211 (41%), Positives = 131/211 (62%), Gaps = 5/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 + +LFDLDGT++NTNELII SF+HTL RE ++ ++G +L++ FS ++ Sbjct: 3 VNTVLFDLDGTLINTNELIIASFLHTLDHYAPGRYKREDVLAFIGPSLYE---TFSSIQK 59 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 A E+ YR++N+ HDEL+ + V E +E LA G KLGIVTTK+R T I L++ Sbjct: 60 DKAEEMITMYRTFNHQMHDELVTEYETVYETLERLAEEGYKLGIVTTKLRDTVIMGLKLT 119 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 L + ET+VT++DV +PKP EPVL A+S LG P +M+GD+ DI + Sbjct: 120 GLDAFFETVVTLDDVERPKPDPEPVLLALSKLGSRPEEAIMIGDNYHDILAGKNAGTKTA 179 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 W++KGE+ L K+EPD+++ M D+ I+ Sbjct: 180 GVAWTIKGEESLAKHEPDFMLQQMSDLLHIL 210 >C2WEB3.1 C2WEB3_BACCE Pyrophosphatase ppaX Length = 212 Score = 169 bits (427), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 85/213 (39%), Positives = 129/213 (60%), Gaps = 5/213 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I +LFDLDGT++NTNELII+SF+HTL + RE ++P++G +LH+ FS +++ Sbjct: 3 INTVLFDLDGTLINTNELIISSFLHTLHKYYPNQYKREDVLPFIGPSLHE---TFSSMDE 59 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 E+ YR +N+ HHDEL+ + V E ++EL +G K+GIVTTK R T L++ Sbjct: 60 GKVDEMIQCYRQFNHDHHDELVEEYETVYETVQELKKQGYKIGIVTTKARQTVEMGLKLS 119 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 L ++ + +VT++DV KPH EP+ KA+ L P TLMVGD+ DI Sbjct: 120 KLNEFFDVVVTIDDVQHVKPHPEPIQKALQLLHAKPEETLMVGDNHHDIVGGQNAGTKTA 179 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIVDQ 212 W++KG + L Y+PDY++ M D+ I+ + Sbjct: 180 AVAWTIKGREYLESYKPDYVLDKMSDLLSILSE 212 >Q8ENK3.1 PPAX_OCEIH Pyrophosphatase ppaX EC=3.6.1.1 Length = 214 Score = 169 bits (427), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 92/211 (43%), Positives = 125/211 (59%), Gaps = 6/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I ILFDLDGT+++TN LI SF HT KE NL + E+I+ + G L + F+ +++ Sbjct: 3 IRTILFDLDGTLIDTNTLIKASFEHTFKEYNLN-FSNEEILKFNGPPL---VDTFNKIDE 58 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 T A + ++R +N HD + +FPHV + +EEL R I LGIV+TK+R T LE+ Sbjct: 59 TKADRMITTFREHNIREHDNFVTAFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELT 118 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 + K+ TI+T +DV KPH EPV A+ LG P TLMVGD+ DI S Sbjct: 119 GISKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMVGDNHHDIVSGQRANVQTA 178 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 WSLK + L+ + PDYII D+KDI IV Sbjct: 179 AVAWSLKDTNYLKSFHPDYIIEDIKDIITIV 209 >C3BSE3.1 C3BSE3_9BACI Pyrophosphatase ppaX Length = 212 Score = 168 bits (425), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 85/213 (39%), Positives = 128/213 (60%), Gaps = 5/213 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I +LFDLDGT++NTNELII+SF+HTL + RE I+P++G +LH+ F +++ Sbjct: 3 INTVLFDLDGTLINTNELIISSFLHTLNKYYPDQYKREDILPFIGPSLHE---TFRSMDE 59 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 E+ YR +N+ HHDEL+ + V E ++EL +G K+GIVTTK R T L++ Sbjct: 60 GKVDEMIQCYRQFNHDHHDELVEEYETVYETVQELKKQGYKIGIVTTKARQTVEMGLKLS 119 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 L ++ + +VT++DV KPH EP+ KA+ L P TLMVGD+ DI Sbjct: 120 KLKEFFDVVVTIDDVEHVKPHPEPIQKALKLLDAKPEETLMVGDNHHDIVGGQNAGTKTA 179 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIVDQ 212 W++KG + L Y+PDY++ M D+ I+ + Sbjct: 180 AVAWTIKGREYLESYKPDYVLDRMSDLLSILSE 212 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,218,485,510 Number of extensions: 46002496 Number of successful extensions: 132120 Number of sequences better than 1.0e-05: 1835 Number of HSP's gapped: 131572 Number of HSP's successfully gapped: 1835 Length of query: 217 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 87 Effective length of database: 1,835,964,253 Effective search space: 159728890011 Effective search space used: 159728890011 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)