BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0002.p (312 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K335.1 B3K335_9BACI HPr(Ser) kinase/phosphatase 566 e-160 Q50EL5.1 Q50EL5_BACST HPr kinase/phosphorylase 536 e-150 B1DKW5.1 B1DKW5_9BACL HPr kinase 508 e-142 B4AGQ2.1 B4AGQ2_BACPU HPr(Ser) kinase/phosphatase EC=2.7.1.- 446 e-123 A8FHS4.1 HPRK_BACP2 HPr kinase/phosphorylase EC=2.7.11.- EC=2.7.4.- 446 e-123 >B3K335.1 B3K335_9BACI HPr(Ser) kinase/phosphatase Length = 312 Score = 566 bits (1459), Expect = e-160, Method: Compositional matrix adjust. Identities = 271/312 (86%), Positives = 293/312 (93%) Query: 1 MAKKVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTE 60 MAKKVKVSELVQ FQLEV++G EGLKR IT DDLNRPGLEMAGYFEY+P ERVQL+G+TE Sbjct: 1 MAKKVKVSELVQQFQLEVIAGEEGLKRQITTDDLNRPGLEMAGYFEYHPRERVQLMGRTE 60 Query: 61 LAFFSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIF 120 LAFF ML EERK R+R +C + TPCIV+TR+L+VP+EL+++ NE+GLPVLRSSMATTI Sbjct: 61 LAFFGMLKPEERKERMRSLCTEETPCIVVTRSLEVPEELVELGNERGLPVLRSSMATTIL 120 Query: 121 SSRLTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAV 180 +SR TSFLE RLAPT TIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAV Sbjct: 121 TSRFTSFLERRLAPTTTIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAV 180 Query: 181 EIRQTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDK 240 EIRQTSD QLHGTAPELIRHLLEIRGVGIINVMTLFGAGA+RN+KRITLVVRLEAWQQDK Sbjct: 181 EIRQTSDFQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAVRNNKRITLVVRLEAWQQDK 240 Query: 241 QYDRLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKL 300 QYDRLGLDEETTRII+TD+PLVTIPVRPGRNLAVIIEVAAMNYRLKQMG NAALQFTNKL Sbjct: 241 QYDRLGLDEETTRIIETDIPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGLNAALQFTNKL 300 Query: 301 TATISEDMDDLD 312 TATI+EDMDDLD Sbjct: 301 TATIAEDMDDLD 312 >Q50EL5.1 Q50EL5_BACST HPr kinase/phosphorylase Length = 316 Score = 536 bits (1380), Expect = e-150, Method: Compositional matrix adjust. Identities = 261/309 (84%), Positives = 287/309 (92%), Gaps = 1/309 (0%) Query: 1 MAKKVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTE 60 MAKKVKVSELV F LEV+SG +GLKR ITVDDL RPGLEMAGYFEY+P ERVQ+LGKTE Sbjct: 1 MAKKVKVSELVNQFGLEVISGEQGLKRAITVDDLYRPGLEMAGYFEYHPPERVQILGKTE 60 Query: 61 LAFFSMLPDEERKSRIRGICN-DNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTI 119 LAFF MLP++ERK R+ +C+ D TPCI++TR+ VP+ELI+IS+EK +PVLRSSMAT I Sbjct: 61 LAFFEMLPEKERKDRMERLCSSDETPCIIVTRSWKVPEELIEISSEKQIPVLRSSMATAI 120 Query: 120 FSSRLTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDA 179 SSR+TSFLE +LAPTATIHGVL DVYGVGMLITGSSGIGKSETALELVKRGHRLIADDA Sbjct: 121 LSSRITSFLERKLAPTATIHGVLVDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDA 180 Query: 180 VEIRQTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQD 239 VEIRQTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAG+IRN+KRI+LVVRLEAWQQ+ Sbjct: 181 VEIRQTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGSIRNNKRISLVVRLEAWQQE 240 Query: 240 KQYDRLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNK 299 KQYDRLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMN+RLK+MG+NAALQFT K Sbjct: 241 KQYDRLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNFRLKRMGYNAALQFTTK 300 Query: 300 LTATISEDM 308 LT TISED+ Sbjct: 301 LTETISEDI 309 >B1DKW5.1 B1DKW5_9BACL HPr kinase Length = 312 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 243/312 (77%), Positives = 275/312 (88%) Query: 1 MAKKVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTE 60 MAKKVKVSELVQ+F +E+VSG +GLKR I VDDL RPGLEMAGYF+Y+P ERVQLLGKTE Sbjct: 1 MAKKVKVSELVQHFSMEIVSGEDGLKRSILVDDLYRPGLEMAGYFDYHPHERVQLLGKTE 60 Query: 61 LAFFSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIF 120 L F S L EER+SR +C+++TPCI++TR L+ P EL++ SN + VLRS++ATTI Sbjct: 61 LTFLSTLTSEERRSRFEQLCHESTPCIILTRGLEAPDELVEYSNMTQIAVLRSNVATTIL 120 Query: 121 SSRLTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAV 180 SR+T FLE +LAPTATIHGVL DVYGVGMLITG SGIGKSETALELVKRGHRL+ADDAV Sbjct: 121 QSRITDFLEKKLAPTATIHGVLVDVYGVGMLITGGSGIGKSETALELVKRGHRLVADDAV 180 Query: 181 EIRQTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDK 240 EIRQTSD QL G+APELIRHLLEIRG+GIINVMTLFGAGA+R KRITLV++LE WQQDK Sbjct: 181 EIRQTSDGQLFGSAPELIRHLLEIRGLGIINVMTLFGAGAVRTSKRITLVIKLENWQQDK 240 Query: 241 QYDRLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKL 300 QYDRLGLDEE TRIIDTDVPLVT+PVRPGRNLAVIIEVAAMN+RLK+MG+NAALQFTNKL Sbjct: 241 QYDRLGLDEEKTRIIDTDVPLVTVPVRPGRNLAVIIEVAAMNFRLKRMGYNAALQFTNKL 300 Query: 301 TATISEDMDDLD 312 T TI+ED DD D Sbjct: 301 TETIAEDTDDFD 312 >B4AGQ2.1 B4AGQ2_BACPU HPr(Ser) kinase/phosphatase EC=2.7.1.- Length = 310 Score = 446 bits (1147), Expect = e-123, Method: Compositional matrix adjust. Identities = 211/307 (68%), Positives = 256/307 (83%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV+ ++++ F LE+VSG EG+ RPIT+ DL+RPG+EMAGYF YYP+ERVQLLGKTEL+F Sbjct: 3 KVRTKDIMEQFHLELVSGEEGINRPITISDLSRPGIEMAGYFTYYPKERVQLLGKTELSF 62 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 F LP+ ERK R+ +C D TP I+++R +VP+ELI+ SNE G+PVLRSS+ TT SSR Sbjct: 63 FEQLPERERKQRMMSLCTDITPAIILSRDREVPKELIEASNENGVPVLRSSLKTTRLSSR 122 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT+FLE +LAPT IHGVL DVYGVG+L+ G SG+GKSETALELVKRGHRL+ADD VEIR Sbjct: 123 LTNFLESKLAPTTAIHGVLVDVYGVGVLLIGKSGVGKSETALELVKRGHRLVADDCVEIR 182 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 Q + L G+APELI HLLEIRG+GIINVMTLFGAGA+R++KRIT+V+ LE W+Q KQYD Sbjct: 183 QEDQDTLVGSAPELIEHLLEIRGLGIINVMTLFGAGAVRSYKRITIVMNLELWEQGKQYD 242 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGL+EE RIIDTDVP +TIPVRPGRNLAVIIEVAAMN+RLK+MG NAA QFT+KL Sbjct: 243 RLGLEEEKMRIIDTDVPKLTIPVRPGRNLAVIIEVAAMNFRLKRMGHNAAEQFTSKLADV 302 Query: 304 ISEDMDD 310 I ++ D Sbjct: 303 IEDNGQD 309 >A8FHS4.1 HPRK_BACP2 HPr kinase/phosphorylase EC=2.7.11.- EC=2.7.4.- Length = 310 Score = 446 bits (1147), Expect = e-123, Method: Compositional matrix adjust. Identities = 211/307 (68%), Positives = 256/307 (83%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV+ ++++ F LE+VSG EG+ RPIT+ DL+RPG+EMAGYF YYP+ERVQLLGKTEL+F Sbjct: 3 KVRTKDIMEQFHLELVSGEEGINRPITISDLSRPGIEMAGYFTYYPKERVQLLGKTELSF 62 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 F LP+ ERK R+ +C D TP I+++R +VP+ELI+ SNE G+PVLRSS+ TT SSR Sbjct: 63 FEQLPERERKQRMMSLCTDITPAIILSRDREVPKELIEASNENGVPVLRSSLKTTRLSSR 122 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT+FLE +LAPT IHGVL DVYGVG+L+ G SG+GKSETALELVKRGHRL+ADD VEIR Sbjct: 123 LTNFLESKLAPTTAIHGVLVDVYGVGVLLIGKSGVGKSETALELVKRGHRLVADDCVEIR 182 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 Q + L G+APELI HLLEIRG+GIINVMTLFGAGA+R++KRIT+V+ LE W+Q KQYD Sbjct: 183 QEDQDTLVGSAPELIEHLLEIRGLGIINVMTLFGAGAVRSYKRITIVMNLELWEQGKQYD 242 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGL+EE RIIDTDVP +TIPVRPGRNLAVIIEVAAMN+RLK+MG NAA QFT+KL Sbjct: 243 RLGLEEEKMRIIDTDVPKLTIPVRPGRNLAVIIEVAAMNFRLKRMGHNAAEQFTSKLADV 302 Query: 304 ISEDMDD 310 I ++ D Sbjct: 303 IEDNGQD 309 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,753,542,120 Number of extensions: 70561665 Number of successful extensions: 216070 Number of sequences better than 1.0e-05: 630 Number of HSP's gapped: 215723 Number of HSP's successfully gapped: 638 Length of query: 312 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 177 Effective length of database: 1,788,859,178 Effective search space: 316628074506 Effective search space used: 316628074506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)