BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0154.p (227 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061073.1| hypothetical protein [Clostridium thermocellu... 241 1e-62 ref|ZP_00060317.1| hypothetical protein [Clostridium thermocellu... 184 2e-45 ref|NP_617120.1| predicted protein [Methanosarcina acetivorans s... 120 2e-26 ref|ZP_00103966.1| hypothetical protein [Desulfitobacterium hafn... 112 5e-24 gb|EAB10499.1| unknown [environmental sequence] 104 2e-21 >ref|ZP_00061073.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 227 Score = 241 bits (615), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 122/228 (53%), Positives = 156/228 (68%), Gaps = 2/228 (0%) Query: 1 MLDSIFSAALTSAELTFTDAVLTIFISIVLGGLISFTYMKTNPGGY-SQSFTLTMXXXXX 59 MLD +A + S LT A LTI +S LG LIS TYMKT+ GY SQ+FTLT+ Sbjct: 1 MLDIALNANINST-LTLGSAFLTIIVSFALGILISVTYMKTHVKGYYSQNFTLTLILIPA 59 Query: 60 XXXXXXXXXGSNVARAFSLAGAFSIIRFRSAPGDPKDITFVLFTMASGLACGVGSFGYAA 119 GSNVARAFSLAGAFSI RFRSA GDPKDI +VLF+MA+GLACG G FGYAA Sbjct: 60 VIAVIILLVGSNVARAFSLAGAFSIARFRSAAGDPKDIAYVLFSMAAGLACGTGLFGYAA 119 Query: 120 LFTLILCVLMFILNHTGFGARKSQQKTLKVTIPENLGYEEAFAEVFDTFNVRYELKKIRT 179 LFT++LCV M +L FGA+KS +K LK+ IPEN+ Y+ AF ++ + Y+L +++T Sbjct: 120 LFTMLLCVFMVVLCKINFGAKKSCEKILKILIPENMNYQGAFDDILKKYTTNYQLCRVKT 179 Query: 180 TELGSLYELVYEVTIDEHTSQKELLDAVRTRNGNLDLSLTMCPPATDY 227 T+LG+L+ELVY VT+D +KE +D +R RNGNL + L+M D+ Sbjct: 180 TDLGTLFELVYTVTMDNEKDEKEFIDELRCRNGNLSIVLSMNAAPNDF 227 >ref|ZP_00060317.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 206 Score = 184 bits (466), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 94/200 (47%), Positives = 127/200 (63%), Gaps = 1/200 (0%) Query: 22 LTIFISIVLGGLISFTYMKTN-PGGYSQSFTLTMXXXXXXXXXXXXXXGSNVARAFSLAG 80 L + +S LG +IS Y+KT+ YSQ+F L + GSNVARAFSLAG Sbjct: 3 LALAVSFALGIIISLVYIKTHLKEHYSQNFALALAIIPSIIAMMIMLIGSNVARAFSLAG 62 Query: 81 AFSIIRFRSAPGDPKDITFVLFTMASGLACGVGSFGYAALFTLILCVLMFILNHTGFGAR 140 AF+IIRFRS KDI +VLF M +GLACGVG GYA LFTL+LC + +L FGA+ Sbjct: 63 AFAIIRFRSEEATFKDIAYVLFAMGAGLACGVGFLGYATLFTLVLCGFLIVLGKVNFGAK 122 Query: 141 KSQQKTLKVTIPENLGYEEAFAEVFDTFNVRYELKKIRTTELGSLYELVYEVTIDEHTSQ 200 KS K LKVT+ ++ Y+ F EVF+ + + YELK+++ + LY L Y V++ + Sbjct: 123 KSPYKVLKVTVRDDFDYKREFEEVFNKYALSYELKRVKAKDEEGLYVLEYLVSLYYDVDE 182 Query: 201 KELLDAVRTRNGNLDLSLTM 220 KE +DA+R RNGNL+++L M Sbjct: 183 KEFIDALRYRNGNLNIALGM 202 >ref|NP_617120.1| predicted protein [Methanosarcina acetivorans str. C2A] gb|AAM05600.1| predicted protein [Methanosarcina acetivorans str. C2A] Length = 247 Score = 120 bits (302), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 72/226 (31%), Positives = 120/226 (53%), Gaps = 1/226 (0%) Query: 2 LDSIFSAALTSAELTFTDAVLTIFISIVLGGLISFTYMKTNPG-GYSQSFTLTMXXXXXX 60 L+ IF+ S T TD V+ + +S +L I + Y +T+ G Y+QS+ T+ Sbjct: 21 LEDIFAFEDLSGTFTITDVVVGLLLSFILLSAIGWLYKRTHRGTSYTQSYVHTLIMMGLV 80 Query: 61 XXXXXXXXGSNVARAFSLAGAFSIIRFRSAPGDPKDITFVLFTMASGLACGVGSFGYAAL 120 GSN+ARAFSL GA SIIRFR+A + +D+ F+ F MA G+A G + A + Sbjct: 81 VDIIMLIVGSNIARAFSLVGALSIIRFRNAVKETRDVGFIFFAMAIGMATGTRFYLLAII 140 Query: 121 FTLILCVLMFILNHTGFGARKSQQKTLKVTIPENLGYEEAFAEVFDTFNVRYELKKIRTT 180 T I+ L+FI+ + +R + + LK+ + + + +E+ F +VF + +L I + Sbjct: 141 ATAIIGTLIFIMYEFDWYSRPAISQILKIQLDKEVDFEKLFGKVFVKYTSSADLIGIDSV 200 Query: 181 ELGSLYELVYEVTIDEHTSQKELLDAVRTRNGNLDLSLTMCPPATD 226 + ELVY V + + ++ E ++A++ NGN + L TD Sbjct: 201 RSDTGTELVYSVILKKKANKHEFIEAIKKLNGNQKVFLITGYNTTD 246 >ref|ZP_00103966.1| hypothetical protein [Desulfitobacterium hafniense] Length = 143 Score = 112 bits (281), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 54/131 (41%), Positives = 90/131 (68%) Query: 86 RFRSAPGDPKDITFVLFTMASGLACGVGSFGYAALFTLILCVLMFILNHTGFGARKSQQK 145 RFRS PG K+I+ + MA GLA G+G G A +FTLIL + +L+ GFG +K+ +K Sbjct: 1 RFRSIPGSAKEISSIFLAMAIGLATGMGFLGIAVMFTLILGLTNLLLDWVGFGEQKNAEK 60 Query: 146 TLKVTIPENLGYEEAFAEVFDTFNVRYELKKIRTTELGSLYELVYEVTIDEHTSQKELLD 205 TL++TIPE L Y F ++F+ + +++L +++TT +GSL++L Y + + + ++EL++ Sbjct: 61 TLRITIPEGLDYSGVFDDLFERYLSKWDLVQVKTTNMGSLFKLDYRILLKDMNKERELIN 120 Query: 206 AVRTRNGNLDL 216 +R RNGNL++ Sbjct: 121 ELRCRNGNLEI 131 >gb|EAB10499.1| unknown [environmental sequence] Length = 221 Score = 104 bits (259), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 60/211 (28%), Positives = 107/211 (50%), Gaps = 1/211 (0%) Query: 18 TDAVLTIFISIVLGGLISFTYMKTNPG-GYSQSFTLTMXXXXXXXXXXXXXXGSNVARAF 76 + + + +S LG LISF Y KT+ G YSQSF +T G+++ARAF Sbjct: 11 NEIIFNLILSFFLGVLISFVYKKTHRGMSYSQSFMITNIFLSLIVCMVIMTIGNSIARAF 70 Query: 77 SLAGAFSIIRFRSAPGDPKDITFVLFTMASGLACGVGSFGYAALFTLILCVLMFILNHTG 136 +L GA SIIRFR+ DPKDI F+ +++A+G+ CG G + A + +LI+ ++ +IL+ T Sbjct: 71 ALVGALSIIRFRTVIKDPKDIVFIFWSLATGMVCGTGQYFLAFVGSLIITIVAYILHKTN 130 Query: 137 FGARKSQQKTLKVTIPENLGYEEAFAEVFDTFNVRYELKKIRTTELGSLYELVYEVTIDE 196 +G+ + L+ ++ E ++ + + F L + + +L +++ + E Sbjct: 131 YGSIYKSEFILQFRYKKSAENETSYLKNLNRFCSVSTLLNSEPSSDNTSLKLTFDIVLKE 190 Query: 197 HTSQKELLDAVRTRNGNLDLSLTMCPPATDY 227 + + + G ++SL DY Sbjct: 191 ENEVNKFVLDISNCEGVSEVSLVAAQSNVDY 221 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 285,170,448 Number of extensions: 10301196 Number of successful extensions: 26733 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 26725 Number of HSP's successfully gapped: 19 Length of query: 227 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 106 Effective length of database: 428,710,599 Effective search space: 45443323494 Effective search space used: 45443323494 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 124 (52.4 bits)