BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0138.p (308 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAK56233.1| unknown [environmental sequence] 258 8e-68 ref|NP_520442.1| PROBABLE DEHYDROGENASE/REDUCTASE OXIDOREDUCTASE... 258 1e-67 ref|ZP_00073277.1| COG0300: Short-chain dehydrogenases of variou... 227 2e-58 ref|ZP_00107009.1| COG0300: Short-chain dehydrogenases of variou... 216 5e-55 ref|NP_486931.1| putative dehydrogenase [Nostoc sp. PCC 7120] >g... 202 1e-50 >gb|EAK56233.1| unknown [environmental sequence] Length = 275 Score = 258 bits (660), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 131/258 (50%), Positives = 172/258 (66%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 + GK +L+TGASSGIG FA LAK G+ L+LVARS+ KL LAAEL R Y + Sbjct: 6 DFHGKTALVTGASSGIGRAFAYALAKRGARLLLVARSRDKLHDLAAELRRDYACDADFLT 65 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS A + + +D+LVNNAGFA +G FE I RQ +EV++N A +++ Sbjct: 66 VDLSTADAVHTIDCHLKVTGKVVDVLVNNAGFAAYGRFETIPWTRQRDEVLVNCMAAIEL 125 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 THL LPGM R AGAVINV+STA FQP PYMAVYGATKAF+LSF++A+W ENR RG++ Sbjct: 126 THLLLPGMQARSAGAVINVASTAAFQPDPYMAVYGATKAFLLSFSEAVWAENRHRGIRVV 185 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISR 288 ALCPG+T+T FF+VVG ++A+VG VVE L A+ + + + + G RN L A + R Sbjct: 186 ALCPGATQTAFFDVVGAQEAAVGTPMPVASVVEDALWALDRNRSHRIVGTRNRLLANLQR 245 Query: 289 FMTRRQSLRLAQGMLSPR 306 ++R S RL + +L PR Sbjct: 246 LLSRETSARLVEKILRPR 263 >ref|NP_520442.1| PROBABLE DEHYDROGENASE/REDUCTASE OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum] emb|CAD16028.1| PROBABLE DEHYDROGENASE/REDUCTASE OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum] Length = 275 Score = 258 bits (660), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 128/258 (49%), Positives = 173/258 (67%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 + +GK +LITGASSGIG +FA LAK G+ L+LVARS+ KL L AEL R Y + Sbjct: 6 DFRGKTALITGASSGIGREFAYALAKRGAKLLLVARSRDKLHDLTAELRRDYACDADFLT 65 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS A + + +D+L+NNAGFAT+G FE I RQ +EV++N A +++ Sbjct: 66 VDLSAPDAIPTVAHLLKATGTVVDVLINNAGFATYGRFETIPWTRQRDEVLVNCMAAIEL 125 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 THL LPGM R GAVINV+STA FQP PYMA+YGATKAF+LSF++A+W ENR RG++ Sbjct: 126 THLLLPGMQARSDGAVINVASTAAFQPDPYMAIYGATKAFLLSFSEAVWAENRHRGIRVL 185 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISR 288 ALCPG+T+T FF+VVG ++A+VG VV+ L A+ + + Y + G +N L A + R Sbjct: 186 ALCPGATQTGFFDVVGAKEAAVGTPMPVASVVQDALWALDRNRSYRIVGTQNRLLANLQR 245 Query: 289 FMTRRQSLRLAQGMLSPR 306 ++R S RL + +L PR Sbjct: 246 LLSREMSARLVEKILRPR 263 >ref|ZP_00073277.1| COG0300: Short-chain dehydrogenases of various substrate specificities [Trichodesmium erythraeum IMS101] Length = 264 Score = 227 bits (579), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 122/253 (48%), Positives = 162/253 (64%), Gaps = 5/253 (1%) Query: 53 KWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLS 112 K +LITGASSGIGE FA++LA ++LVLVARS+ KL LA ++ Y IQ V+ QDL+ Sbjct: 2 KTALITGASSGIGETFAKELAARKTNLVLVARSEDKLHKLANQVESKYQIQTNVIVQDLT 61 Query: 113 IEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLF 172 AA +++ + L IDLLVNNAGF +G F Q E+Q + LN+ A+V++T+LF Sbjct: 62 ASAAAKSIFEVVLEQGLTIDLLVNNAGFGDYGNFTQRPLEKQVNMIQLNITALVELTYLF 121 Query: 173 LPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCP 232 L M R +G +INV+S GFQPLPYM+VYGATKAFVLSF++ALW ENRD GV+ FALCP Sbjct: 122 LGRMQQRQSGGIINVASIGGFQPLPYMSVYGATKAFVLSFSEALWAENRDSGVKIFALCP 181 Query: 233 GSTETNFFNVVG----TEDASVGAKDTPERVVEVTLRAMKKGKQYVVPG-LRNYLGAQIS 287 G TE++FF + S + + VV L A++K K VV G N + S Sbjct: 182 GPTESDFFKKAEFPEFAREGSEAKLTSAQNVVRDALEALEKDKSNVVTGEFLNQVIVNTS 241 Query: 288 RFMTRRQSLRLAQ 300 RF+ R + L + Sbjct: 242 RFLPRETLVSLVE 254 >ref|ZP_00107009.1| COG0300: Short-chain dehydrogenases of various substrate specificities [Nostoc punctiforme] Length = 258 Score = 216 bits (550), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 113/243 (46%), Positives = 156/243 (64%), Gaps = 4/243 (1%) Query: 55 SLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLSIE 114 +LITGASSGIG+ FA++LA ++LVLVARSK KL LA +L + IQ V+ QDL+ Sbjct: 4 ALITGASSGIGKAFAQELAARNTNLVLVARSKEKLSQLAKQLQEQHKIQVDVIIQDLTEP 63 Query: 115 GAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLFLP 174 AA ++ + L IDLL+NNAGF +G F + GERQ + V LN+ A+VD+TH FLP Sbjct: 64 NAAATVFDATKAKGLTIDLLINNAGFGYYGDFAEGDGERQIKIVQLNILALVDLTHKFLP 123 Query: 175 GMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCPGS 234 M R +G++INVSS FQP+PY++VY A+KAF+LSF++ALW ENR GV+ CPG Sbjct: 124 LMRQRRSGSIINVSSITAFQPIPYLSVYAASKAFILSFSEALWAENRQYGVRILVTCPGP 183 Query: 235 TETNFFNVVGTEDASVGAKD---TPERVVEVTLRAMKKGKQYVVPG-LRNYLGAQISRFM 290 ETNFF A + D + E+VV L A++KG V+ + +++SR + Sbjct: 184 IETNFFAEANFPPALASSTDKVYSSEKVVWECLDALEKGYPTVISSDTSTQIRSKLSRLV 243 Query: 291 TRR 293 R+ Sbjct: 244 PRK 246 >ref|NP_486931.1| putative dehydrogenase [Nostoc sp. PCC 7120] pir||AD2167 hypothetical protein all2891 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB74590.1| ORF_ID:all2891~putative dehydrogenase [Nostoc sp. PCC 7120] Length = 258 Score = 202 bits (513), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 105/248 (42%), Positives = 154/248 (62%), Gaps = 4/248 (1%) Query: 55 SLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLSIE 114 +LITGAS GIG+ FA++LA ++LVLVARS+ KL LA EL + + IQ V+ +DL+ Sbjct: 4 ALITGASGGIGKAFAQELAARQTNLVLVARSQHKLHQLAQELQQQHKIQVDVIAKDLTEI 63 Query: 115 GAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLFLP 174 A +++ + L ID L+NNAGF +G F Q RQ + + LNV A+VD+TH FLP Sbjct: 64 DAVADVFDITKSKGLTIDCLINNAGFGDYGDFAQSDRTRQIKIIQLNVLALVDLTHRFLP 123 Query: 175 GMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCPGS 234 M +G++INV+S AGFQP+PY++VY A+KAF++SF++ALW ENR GV+ CPG Sbjct: 124 LMRQNRSGSIINVASIAGFQPIPYLSVYAASKAFIVSFSEALWAENRQYGVRVLVTCPGP 183 Query: 235 TETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQY----VVPGLRNYLGAQISRFM 290 TET+FF A V + EV L+++K + + ++ L + ++R + Sbjct: 184 TETDFFAEANFPQALVETTNKVMSSEEVVLKSLKALENWEPTVIISDTSTQLRSNVARLV 243 Query: 291 TRRQSLRL 298 R+ L L Sbjct: 244 PRKTLLSL 251 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 418,341,951 Number of extensions: 16649060 Number of successful extensions: 62384 Number of sequences better than 1.0e-05: 5622 Number of HSP's gapped: 58278 Number of HSP's successfully gapped: 5625 Length of query: 308 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 183 Effective length of database: 417,850,203 Effective search space: 76466587149 Effective search space used: 76466587149 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)