BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0128.p (575 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P08838|PT1_BACSU Phosphoenolpyruvate-protein phosphotransfera... 894 0.0 sp|P42014|PT1_BACST Phosphoenolpyruvate-protein phosphotransfera... 875 0.0 sp|O83018|PT1_BACSP Phosphoenolpyruvate-protein phosphotransfera... 875 0.0 ref|NP_389274.1| phosphotransferase system (PTS) enzyme I [Bacil... 852 0.0 sp|O69251|PT1_BACME Phosphoenolpyruvate-protein phosphotransfera... 848 0.0 >sp|P08838|PT1_BACSU Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase system, enzyme I) pir||C46238 phosphotransferase system enzyme I (EC 2.7.3.9) - Bacillus subtilis gb|AAB52374.1| enzyme I emb|CAB13264.2| phosphotransferase system (PTS) enzyme I [Bacillus subtilis subsp. subtilis str. 168] Length = 570 Score = 894 bits (2310), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/551 (77%), Positives = 487/551 (88%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLEEP+L + KKN+ D EAE R ++AI+RSK ELEKI E+ ELG DKA+I +A LL Sbjct: 19 YRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEHALKELGQDKADIFSAHLL 78 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNPV EKIST+ VNAEFALKE++ FV+M ESMDNEYM+ERA DIRDVT RVT Sbjct: 79 VLSDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDNEYMKERAADIRDVTKRVT 138 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 HLLGV+I +PS+ISEEVII+A DLTPSDTAQLNR +VKGFTTDIGGRTSHSAIMARS+E Sbjct: 139 GHLLGVEIPNPSMISEEVIIVAEDLTPSDTAQLNREFVKGFTTDIGGRTSHSAIMARSLE 198 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK A I+NG+ VIVDG+ G+VIIDPS+E + YE K Y AQKA WAK+V+ Sbjct: 199 IPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYEEKHNAYLAQKAEWAKLVN 258 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 EPTV+KDG HVELAANIGTP DVKGVL NGGEAVGLYRTEFLYMG+D+LPTE+EQF+AYK Sbjct: 259 EPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYK 318 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GK VVVRTLDIGGDKELPYL LPKEMNPFLG+RAIRLCLEEQ+IFRTQLRALL Sbjct: 319 TVLERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALL 378 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA +YGNLKIMFPMIATV+EF+EAKAIL+EEKEKL+ +G +D+IE+GMMVEIPSTAV+ Sbjct: 379 RASTYGNLKIMFPMIATVNEFKEAKAILLEEKEKLVKAGQAVSDDIEVGMMVEIPSTAVI 438 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRL+++VI+AAHKEGKW Sbjct: 439 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLITLVIEAAHKEGKW 498 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIP+L+ LGLDEFSMSATSILPAR QI +LS + A S KE +LSM T+ Sbjct: 499 VGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKQEAESFKEKILSMSTT 558 Query: 560 EEVIEYVKRTF 570 EEV+ +VK TF Sbjct: 559 EEVVAFVKETF 569 >sp|P42014|PT1_BACST Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase system, enzyme I) gb|AAA86049.1| PTS enzyme I Length = 578 Score = 875 bits (2261), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/553 (75%), Positives = 479/553 (86%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLE P+L K+ V D EAE RLE A++++K ELE I ++ +LG DKA I AA LL Sbjct: 25 YRLETPDLAAEKRTVADVEAEIARLEAAVAKAKEELEAIKQHALEKLGEDKAAIFAAHLL 84 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNP+ EKI TE VNAE+AL E+A F+SM E+MDNEYM+ERA DIRDVT RV Sbjct: 85 VLDDPELLNPIKEKIQTERVNAEYALDETALFFISMFEAMDNEYMKERAADIRDVTKRVL 144 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 AHLLGV IS+PSLISEEV+IIA DLTPSDTAQLNR+YVKGF TDIGGRTSHSAIMARS+E Sbjct: 145 AHLLGVTISNPSLISEEVVIIAEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLE 204 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK + ++NG +VIVDGL+G+VII+PS E++A YE K+ +YEAQKA WAK+VH Sbjct: 205 IPAVVGTKTVTAEVKNGDIVIVDGLDGQVIINPSPELLAQYEQKRARYEAQKAEWAKLVH 264 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 E TVT DG+HVELAANIGTP DVKG LANG E +GLYRTEFLYMG+ LPTE+EQF AYK Sbjct: 265 EATVTADGIHVELAANIGTPDDVKGALANGAEGIGLYRTEFLYMGRSELPTEDEQFVAYK 324 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GKPVVVRTLDIGGDKELPYL LPKEMNPFLGFRAIRLCLE QD+FRTQLRALL Sbjct: 325 TVLEQMNGKPVVVRTLDIGGDKELPYLQLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALL 384 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA YGNLKIMFPMIAT+ EFR+AKAIL+EEKE L+ GV AD IE+GMMVEIP+ AVM Sbjct: 385 RASVYGNLKIMFPMIATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVM 444 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERV+YLYQPYNPAILRL+S VIDAAH+EGKW Sbjct: 445 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREGKW 504 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGDPIAIP+L++LGLDEFSMSATSILPARAQ+K+L+ E A +KE VLS+ T+ Sbjct: 505 VGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKKLAKEEAARIKETVLSLGTA 564 Query: 560 EEVIEYVKRTFDI 572 EEV+ +VKRTF + Sbjct: 565 EEVVSFVKRTFSL 577 >sp|O83018|PT1_BACSP Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase system, enzyme I) dbj|BAA31955.1| phosphotransferase system enzyme I [Bacillus sp.] Length = 578 Score = 875 bits (2260), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/553 (75%), Positives = 478/553 (86%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLE P+L K+ V D EAE R E A++++K ELE I ++ +LG DKA I AA LL Sbjct: 25 YRLETPDLAAEKRAVADVEAEVARFEAAVAKAKEELEAIKQHALEKLGEDKAAIFAAHLL 84 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNP+ EKI TE VNAE++L E+A F+SM E+MDNEYM+ERA DIRDVT RV Sbjct: 85 VLDDPELLNPIKEKIQTERVNAEYSLDETASFFISMFEAMDNEYMKERAADIRDVTKRVL 144 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 AHLLGV IS+PSLISEEV+IIA DLTPSDTAQLNR+YVKGF TDIGGRTSHSAIMARS+E Sbjct: 145 AHLLGVTISNPSLISEEVVIIAEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLE 204 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK + ++NG +VIVDGL+G+VII+PS E++A YE K+ +YEAQKA WAK+VH Sbjct: 205 IPAVVGTKTVTAEVKNGDIVIVDGLDGQVIINPSPELLAQYEQKRARYEAQKAEWAKLVH 264 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 E TVT DG+HVELAANIGTP DVKG LANG E +GLYRTEFLYMG+ LPTE+EQF AYK Sbjct: 265 EATVTADGIHVELAANIGTPDDVKGALANGAEGIGLYRTEFLYMGRSELPTEDEQFVAYK 324 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GKPVVVRTLDIGGDKELPYL LPKEMNPFLGFRAIRLCLE QD+FRTQLRALL Sbjct: 325 TVLEQMNGKPVVVRTLDIGGDKELPYLQLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALL 384 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA YGNLKIMFPMIAT+ EFR+AKAIL+EEKE L+ GV AD IE+GMMVEIP+ AVM Sbjct: 385 RASVYGNLKIMFPMIATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVM 444 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERV+YLYQPYNPAILRL+S VIDAAH+EGKW Sbjct: 445 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREGKW 504 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGDPIAIP+L++LGLDEFSMSATSILPARAQ+K+L+ E A +KE VLS+ T+ Sbjct: 505 VGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKQLAKEEAARIKETVLSLGTA 564 Query: 560 EEVIEYVKRTFDI 572 EEV+ +VKRTF + Sbjct: 565 EEVVSFVKRTFSL 577 >ref|NP_389274.1| phosphotransferase system (PTS) enzyme I [Bacillus subtilis] Length = 550 Score = 852 bits (2202), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/551 (74%), Positives = 469/551 (85%), Gaps = 20/551 (3%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLEEP+L + KKN+ D EAE R ++AI+RSK ELEKI E+ ELG Sbjct: 19 YRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEHALKELG------------ 66 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 PV EKIST+ VNAEFALKE++ FV+M ESMDNEYM+ERA DIRDVT RVT Sbjct: 67 --------QPVKEKISTDSVNAEFALKETSSMFVTMFESMDNEYMKERAADIRDVTKRVT 118 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 HLLGV+I +PS+ISEEVII+A DLTPSDTAQLNR +VKGFTTDIGGRTSHSAIMARS+E Sbjct: 119 GHLLGVEIPNPSMISEEVIIVAEDLTPSDTAQLNREFVKGFTTDIGGRTSHSAIMARSLE 178 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK A I+NG+ VIVDG+ G+VIIDPS+E + YE K Y AQKA WAK+V+ Sbjct: 179 IPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYEEKHNAYLAQKAEWAKLVN 238 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 EPTV+KDG HVELAANIGTP DVKGVL NGGEAVGLYRTEFLYMG+D+LPTE+EQF+AYK Sbjct: 239 EPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYK 298 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GK VVVRTLDIGGDKELPYL LPKEMNPFLG+RAIRLCLEEQ+IFRTQLRALL Sbjct: 299 TVLERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALL 358 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA +YGNLKIMFPMIATV+EF+EAKAIL+EEKEKL+ +G +D+IE+GMMVEIPSTAV+ Sbjct: 359 RASTYGNLKIMFPMIATVNEFKEAKAILLEEKEKLVKAGQAVSDDIEVGMMVEIPSTAVI 418 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRL+++VI+AAHKEGKW Sbjct: 419 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLITLVIEAAHKEGKW 478 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIP+L+ LGLDEFSMSATSILPAR QI +LS + A S KE +LSM T+ Sbjct: 479 VGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKQEAESFKEKILSMSTT 538 Query: 560 EEVIEYVKRTF 570 EEV+ +VK TF Sbjct: 539 EEVVAFVKETF 549 >sp|O69251|PT1_BACME Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase system, enzyme I) emb|CAA06332.1| enzyme I [Bacillus megaterium] Length = 573 Score = 848 bits (2192), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/553 (74%), Positives = 471/553 (85%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 +RLE PEL + KK+V + EAE RLE A+ +SK+ELE I E+ ELG DKAEI A LL Sbjct: 20 FRLENPELTVEKKSVTEVEAEVARLEAALEKSKSELEIIREHARKELGDDKAEIFEAHLL 79 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPEL+NP+ +KI+ E VNAE AL E A F++M ESMDNEYM+ERA DIRDVT RV Sbjct: 80 VLSDPELINPIKDKITNENVNAEHALDEVAAMFINMFESMDNEYMKERAADIRDVTKRVL 139 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 AHLLGV +S+PSLISEEV+IIA DLTPSDTAQLNR++VKGFTTDIGGRTSHSAIMARSME Sbjct: 140 AHLLGVNVSNPSLISEEVVIIAEDLTPSDTAQLNRKFVKGFTTDIGGRTSHSAIMARSME 199 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK + I+NG++VIVDGL+GEVI+DPS E + YE K +Y QKA WAK+V+ Sbjct: 200 IPAVVGTKTVMEDIQNGVLVIVDGLDGEVIVDPSEETVKAYEKKAAEYAEQKAEWAKLVN 259 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 E TV+ D HVELAANIGTP DVKGVL NGGE VGLYRTEFLYMG++ LPTEEEQF +YK Sbjct: 260 EKTVSADDHHVELAANIGTPEDVKGVLENGGEGVGLYRTEFLYMGREDLPTEEEQFTSYK 319 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLE QD+FRTQLRALL Sbjct: 320 TVLERMEGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALL 379 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA YGNLKIMFPMIATV EFR+AKAIL+EEK KL GV+ +++IE+GMMVEIPS+AV+ Sbjct: 380 RASVYGNLKIMFPMIATVDEFRQAKAILLEEKAKLQQEGVQVSEDIEVGMMVEIPSSAVI 439 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 AD FAKEVDF SIGTNDLIQYT+AADRMNERVSYLYQPYNPAILRLV+MVI AAHKEGKW Sbjct: 440 ADLFAKEVDFFSIGTNDLIQYTLAADRMNERVSYLYQPYNPAILRLVNMVIKAAHKEGKW 499 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIP+L+ LGLDEFSMSATSIL AR+QI++LS + +LSM ++ Sbjct: 500 VGMCGEMAGDEIAIPILLGLGLDEFSMSATSILKARSQIRQLSKADIEPHLDTILSMSST 559 Query: 560 EEVIEYVKRTFDI 572 EEVIE+VK TF I Sbjct: 560 EEVIEFVKSTFHI 572 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 751,017,420 Number of extensions: 30341098 Number of successful extensions: 105158 Number of sequences better than 1.0e-05: 938 Number of HSP's gapped: 103246 Number of HSP's successfully gapped: 966 Length of query: 575 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 444 Effective length of database: 401,559,609 Effective search space: 178292466396 Effective search space used: 178292466396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)