BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0127.p (93 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_389273.1| histidine-containing phosphocarrier protein of ... 103 9e-22 sp|O69250|PTHP_BACME Phosphocarrier protein HPr (Histidine-conta... 103 1e-21 pdb|1SPH|A Chain A, Phosphocarrier Protein (Hpr, Histidine-Conta... 102 3e-21 ref|ZP_00046904.1| COG1925: Phosphotransferase system, HPr-relat... 101 5e-21 ref|NP_964671.1| phosphocarrier protein HPr [Lactobacillus johns... 101 6e-21 >ref|NP_389273.1| histidine-containing phosphocarrier protein of the phosphotransferase system (PTS) (HPr protein) [Bacillus subtilis] sp|P08877|PTHP_BACSU Phosphocarrier protein HPr (Histidine-containing protein) pir||WQBSPH phosphotransferase system phosphohistidine-containing protein [validated] - Bacillus subtilis emb|CAA31317.1| Hpr protein [Bacillus subtilis] emb|CAB13263.1| histidine-containing phosphocarrier protein of the phosphotransferase system (PTS) (HPr protein) [Bacillus subtilis subsp. subtilis str. 168] Length = 88 Score = 103 bits (258), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 48/88 (54%), Positives = 63/88 (71%) Query: 1 MVKKTFTVTDELGIHTRPASILVQAASKYNTEVNIEYNGRTVNLKSIVGVMSLGVPXXXX 60 M +KTF VT + GIH RPA++LVQ ASKY+ +VN+EYNG+TVNLKSI+GVMSLG+ Sbjct: 1 MAQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAE 60 Query: 61 XXXXXXXSDADKAIEGIEEALKKEGLGE 88 +D + A+ +EE +K EGLGE Sbjct: 61 ITISASGADENDALNALEETMKSEGLGE 88 >sp|O69250|PTHP_BACME Phosphocarrier protein HPr (Histidine-containing protein) emb|CAA06331.1| HPr protein [Bacillus megaterium] Length = 88 Score = 103 bits (257), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 47/88 (53%), Positives = 64/88 (72%) Query: 1 MVKKTFTVTDELGIHTRPASILVQAASKYNTEVNIEYNGRTVNLKSIVGVMSLGVPXXXX 60 M +KTFTVT + GIH RPA+ LVQAASK+++++N+E+NG+TVNLKSI+GVMSLG+ Sbjct: 1 MAQKTFTVTADSGIHARPATTLVQAASKFDSDINLEFNGKTVNLKSIMGVMSLGIQKGAT 60 Query: 61 XXXXXXXSDADKAIEGIEEALKKEGLGE 88 SD A+ +E+ + KEGLGE Sbjct: 61 ITISAEGSDEADALAALEDTMSKEGLGE 88 >pdb|1SPH|A Chain A, Phosphocarrier Protein (Hpr, Histidine-Containing Protein) Mutant With Ser A 46 Replaced By Ala, Ser B 46 Replaced By Ala (S(46 A)a,S(46 B)a) pdb|1SPH|B Chain B, Phosphocarrier Protein (Hpr, Histidine-Containing Protein) Mutant With Ser A 46 Replaced By Ala, Ser B 46 Replaced By Ala (S(46 A)a,S(46 B)a) Length = 88 Score = 102 bits (254), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 47/88 (53%), Positives = 62/88 (70%) Query: 1 MVKKTFTVTDELGIHTRPASILVQAASKYNTEVNIEYNGRTVNLKSIVGVMSLGVPXXXX 60 M +KTF VT + GIH RPA++LVQ ASKY+ +VN+EYNG+TVNLK I+GVMSLG+ Sbjct: 1 MAQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKDIMGVMSLGIAKGAE 60 Query: 61 XXXXXXXSDADKAIEGIEEALKKEGLGE 88 +D + A+ +EE +K EGLGE Sbjct: 61 ITISASGADENDALNALEETMKSEGLGE 88 >ref|ZP_00046904.1| COG1925: Phosphotransferase system, HPr-related proteins [Lactobacillus gasseri] Length = 88 Score = 101 bits (252), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 48/88 (54%), Positives = 63/88 (71%) Query: 1 MVKKTFTVTDELGIHTRPASILVQAASKYNTEVNIEYNGRTVNLKSIVGVMSLGVPXXXX 60 M K+ F + E GIH RPA++LVQAASK+N+++N+EYNG++VNLKSI+GVMSLGV Sbjct: 1 MEKREFHIVAETGIHARPATLLVQAASKFNSDINLEYNGKSVNLKSIMGVMSLGVGQGAD 60 Query: 61 XXXXXXXSDADKAIEGIEEALKKEGLGE 88 DA +AIE I + +KKEGL E Sbjct: 61 VTITADGDDAKEAIEAIADTMKKEGLAE 88 >ref|NP_964671.1| phosphocarrier protein HPr [Lactobacillus johnsonii NCC 533] gb|AAS08637.1| phosphocarrier protein HPr [Lactobacillus johnsonii NCC 533] Length = 88 Score = 101 bits (251), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 48/88 (54%), Positives = 63/88 (71%) Query: 1 MVKKTFTVTDELGIHTRPASILVQAASKYNTEVNIEYNGRTVNLKSIVGVMSLGVPXXXX 60 M K+ F + E GIH RPA++LVQAASK+N+++N+EYNG++VNLKSI+GVMSLGV Sbjct: 1 MEKREFHIIAETGIHARPATLLVQAASKFNSDINLEYNGKSVNLKSIMGVMSLGVGQGAD 60 Query: 61 XXXXXXXSDADKAIEGIEEALKKEGLGE 88 DA +AIE I + +KKEGL E Sbjct: 61 VTITADGDDAKEAIEAIADTMKKEGLAE 88 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.310 0.130 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 107,132,418 Number of extensions: 2928439 Number of successful extensions: 4611 Number of sequences better than 1.0e-05: 102 Number of HSP's gapped: 4617 Number of HSP's successfully gapped: 102 Length of query: 93 Length of database: 757,237,578 Length adjustment: 61 Effective length of query: 32 Effective length of database: 591,616,539 Effective search space: 18931729248 Effective search space used: 18931729248 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 120 (50.8 bits)