BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0119.p (236 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAC80682.1| hypothetical protein [Listeria grayi] 291 1e-77 ref|NP_831486.1| Short chain dehydrogenase [Bacillus cereus ATCC... 182 6e-45 ref|ZP_00184430.1| hypothetical protein Exigu013301 [Exiguobacte... 174 1e-42 gb|AAR38179.1| oxidoreductase, short chain dehydrogenase/reducta... 147 3e-34 ref|ZP_00070529.1| COG1028: Dehydrogenases with different specif... 141 1e-32 >emb|CAC80682.1| hypothetical protein [Listeria grayi] Length = 239 Score = 291 bits (744), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 149/239 (62%), Positives = 169/239 (70%), Gaps = 3/239 (1%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 MTK ALVTGANKGIG E R+L GWKV+LGAR E+GK AV EL +QGLDV FL+ID+ Sbjct: 1 MTKSALVTGANKGIGLETVRRLAGKGWKVVLGARDAEKGKQAVTELQAQGLDVAFLEIDL 60 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 ES+E A + YP ++LLINNAGMPG F+ FS T EE LR AFEVNFFGTFRLN Sbjct: 61 VDKESVESAVKIITQEYPDISLLINNAGMPGEFAKGFSKTTEEGLRNAFEVNFFGTFRLN 120 Query: 121 QRLLPLITENDGTIVNVSTDMASLHFM---QNAEYALNAFDYNSSKTANNAMTVAMAIEL 177 Q LLP + N TIVNVS DMASL M Q ALN+FDYN+SKTANNAMTV+MA+EL Sbjct: 121 QLLLPSLKGNGATIVNVSIDMASLSLMMDHQERTAALNSFDYNASKTANNAMTVSMALEL 180 Query: 178 KNSKAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 K+S AQVFAVTPGFT TDLN II+ + DG+RHNG F D NGV AW Sbjct: 181 KDSPAQVFAVTPGFTTTDLNNNAPGGITKEEAAGIILKHTLDGERHNGVFFDKNGVLAW 239 >ref|NP_831486.1| Short chain dehydrogenase [Bacillus cereus ATCC 14579] gb|AAP08687.1| Short chain dehydrogenase [Bacillus cereus ATCC 14579] Length = 236 Score = 182 bits (462), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 102/238 (42%), Positives = 144/238 (60%), Gaps = 4/238 (1%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 M K A +TGANKGIG+E+ RQL E + V LGAR+ + G+ AV L +V ++Q+D+ Sbjct: 1 MKKYAFITGANKGIGYELVRQLAEKDYHVFLGARNEQLGQQAVESLNVS--NVSYIQVDI 58 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 ++ +SI++A + L LLINNAG+ F++ S E LR+ FEVNFFGTF++ Sbjct: 59 SNSQSIQEAIKKIYEMTDHLHLLINNAGIALDFNTLPSELNIETLRQGFEVNFFGTFQMM 118 Query: 121 QRLLPLI-TENDGTIVNVSTDMASLHFMQNAE-YALNAFDYNSSKTANNAMTVAMAIELK 178 Q LPL+ ++ I+NV+TDMASL N E + +N YNSSKTA NA+T+A + E Sbjct: 119 QAFLPLLKNSSNSKIINVTTDMASLTMFANGETHPINTLGYNSSKTAINALTLAFSKEFA 178 Query: 179 NSKAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 + +VF VTPGFT TDLN II+ YA +NG+ L+ +G+ W Sbjct: 179 TNGPEVFGVTPGFTTTDLNGNSPGGHTTTESAKIIIKYALSETNYNGKILNKDGIIPW 236 >ref|ZP_00184430.1| hypothetical protein Exigu013301 [Exiguobacterium sp. 255-15] Length = 234 Score = 174 bits (442), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 100/236 (42%), Positives = 138/236 (58%), Gaps = 5/236 (2%) Query: 2 TKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDMT 61 T+ ALVTGANKGIG EI RQL E + V L AR G++AVA L S V F+Q+D+T Sbjct: 3 TQYALVTGANKGIGLEIVRQLAEQDYHVFLAARDEANGQSAVASLASD--KVTFVQLDVT 60 Query: 62 SLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLNQ 121 SIE A + L LLINNAG+ F + + LR+ F+VN+FGTF++ Q Sbjct: 61 DPASIEHAKQQILAVTDRLNLLINNAGIALDFGQTPITVDVDVLRQIFDVNYFGTFQVTQ 120 Query: 122 RLLPLITENDGTIVNVSTDMAS-LHFMQNAEYALNAFDYNSSKTANNAMTVAMAIELKNS 180 PL++ +G I+NV+TDM+S F + + LN YNSSKTA NA+T+++ + + + Sbjct: 121 TFYPLLS--NGKIINVTTDMSSQTSFAEGRIHPLNVLGYNSSKTALNAITLSLGTQFQEN 178 Query: 181 KAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 ++F VTPGFT TDLN +IV YA D +NG+ L+ G+ W Sbjct: 179 GPEIFGVTPGFTSTDLNGNAPGGKTTAEGAAVIVRYALDETNYNGKILNDQGIMPW 234 >gb|AAR38179.1| oxidoreductase, short chain dehydrogenase/reductase family [uncultured bacterium 580] Length = 243 Score = 147 bits (370), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 78/203 (38%), Positives = 128/203 (63%), Gaps = 7/203 (3%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 M K+A ++GAN+GIGFE +++L E G KV+LG+R + +GK A+ EL +QG++ + +Q D Sbjct: 1 MEKVAFISGANRGIGFETSKKLAEKGIKVILGSRDMVKGKKALEELAAQGIEADLIQYDA 60 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGM---PGAFSSSFSNTQEEHLREAFEVNFFGTF 117 LE+ ++ + + Y L +L+NNAG+ F ++ + ++ ++E F+ N F Sbjct: 61 ADLEAPQKVYDYILDKYNKLDILVNNAGVLLTGNLFVTNSTTVSDKDIKETFQTNLFSVI 120 Query: 118 RLNQRLLPLITEND-GTIVNVSTDMASLHFMQNAEYALNA---FDYNSSKTANNAMTVAM 173 L Q LLPLI +++ G IVNVST ++SL + + F YN+SKTA NA T+ + Sbjct: 121 SLTQALLPLIKKSEAGRIVNVSTILSSLTLHSAKDSPITPAKEFAYNASKTALNAFTIHL 180 Query: 174 AIELKNSKAQVFAVTPGFTKTDL 196 A+ELK++ +V + PG+ KT+L Sbjct: 181 ALELKDTNIKVNSGHPGWVKTEL 203 >ref|ZP_00070529.1| COG1028: Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) [Oenococcus oeni MCW] Length = 254 Score = 141 bits (355), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 77/199 (38%), Positives = 113/199 (56%), Gaps = 9/199 (4%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 MTKI L+TGANKGIGFE A+Q+G+ GW +L+ R+ ERGK AV +L G+ E+LQID+ Sbjct: 12 MTKIVLITGANKGIGFETAKQIGKNGWTLLISTRNEERGKNAVHQLQQDGITAEWLQIDL 71 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 + SI +AA + YP L LINNAG+ G + + L + +VNF G F + Sbjct: 72 NDIASIHKAARYVEERYPKLDGLINNAGISGDMQKNPLELKSSELEQLAKVNFLGNFEMI 131 Query: 121 QRLLPLITENDGTIVNVSTDMASLHFMQNAEYALNAFDYNSSKTANNAMTVAMAIELKNS 180 + P+++ N G I+NV+ + + N Y +SK + N+M + A+ K Sbjct: 132 KSFTPILSRNHGRILNVTIPATPIG-------SFNPLGYLASKASLNSMIKSFAMYYKKH 184 Query: 181 K--AQVFAVTPGFTKTDLN 197 + ++F V PG TDLN Sbjct: 185 EIPVEIFGVLPGGISTDLN 203 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 299,256,302 Number of extensions: 10583637 Number of successful extensions: 50194 Number of sequences better than 1.0e-05: 4466 Number of HSP's gapped: 48679 Number of HSP's successfully gapped: 4471 Length of query: 236 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 114 Effective length of database: 425,995,500 Effective search space: 48563487000 Effective search space used: 48563487000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)