BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0103.p (395 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P16468|MAOX_BACST NAD-dependent malic enzyme (NAD-ME) >gi|659... 503 e-141 ref|ZP_00187153.1| hypothetical protein Rxyl189801 [Rubrobacter ... 435 e-120 gb|EAI09112.1| unknown [environmental sequence] 416 e-115 ref|NP_863831.1| NAD-dependent malic enzyme [Pirellula sp. 1] >g... 413 e-114 ref|NP_627175.1| putative malate oxidoreductase [Streptomyces co... 408 e-113 >sp|P16468|MAOX_BACST NAD-dependent malic enzyme (NAD-ME) pir||DEBSXS malate dehydrogenase (oxaloacetate-decarboxylating) (EC 1.1.1.38) - Bacillus stearothermophilus gb|AAA22585.1| malic enzyme (EC 1.1.1.38) Length = 478 Score = 503 bits (1296), Expect = e-141, Method: Compositional matrix adjust. Identities = 238/387 (61%), Positives = 303/387 (78%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +++++DR FL H GGKI T +P+ DDL+R+YTPGVA+VCTAI EDP + Y+LT+KR Sbjct: 80 IVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKR 139 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVV+DGTAVLGLG+IGPYAA PVMEGKA+LFK+ AG++AFPICLDT DTEEII+ VK Sbjct: 140 NTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLDTKDTEEIIQIVK 199 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 AIAP FGGINLEDIS+PRCF PVFHDDQHGTA+V+LAGL+NALK+ K+ Sbjct: 200 AIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKK 259 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++V+ G+GAAG++ ++LLAAG R+++ VD+ GA+ R Y +P WQ A + + Sbjct: 260 LEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQEYAQLTNPD 319 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + G+L +VIAGADVFIGVS GIL+ +DV++MA PIVFAMANP PEI PELA PY V Sbjct: 320 NLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDPELAEPYVRV 379 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ IN++MKL VV + ELN YII Sbjct: 380 MATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTEDELNETYII 439 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+FN +VV +VRQAV+EAA +G+A+ Sbjct: 440 PSVFNSKVVERVRQAVVEAAYRTGVAR 466 >ref|ZP_00187153.1| hypothetical protein Rxyl189801 [Rubrobacter xylanophilus DSM 9941] Length = 481 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 218/387 (56%), Positives = 284/387 (73%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +++++DR FL H GGKI +P+ DDL+ YTPGVA+VC AI ++PE+ + LT+KR Sbjct: 80 VVNVSDRTFLLHLGGKIEVHSKIPVRTRDDLSMAYTPGVARVCRAIAQEPERAFNLTIKR 139 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVVTDGTAVLGLG+IGPYAA PVMEGKA+LFK+ AG++AFP+CLDT D EEI+RTVK Sbjct: 140 NSVAVVTDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPVCLDTKDPEEIVRTVK 199 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 +AP FGGINLEDIS+PRCF PVFHDDQHGTA+VVLA LINAL++ GK+ Sbjct: 200 YVAPAFGGINLEDISAPRCFEIEERLKQELDIPVFHDDQHGTAVVVLAALINALRIVGKR 259 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++VV GVGAAGV+ ++LLAAG +++V D+ G + RG A W A + E Sbjct: 260 MEELKVVVCGVGAAGVACAKILLAAGVKNIVGCDRYGVVYRGRGDADTSRGWFAENTNPE 319 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + GTL + I GADVFIGVS +L + ++ MA PIVFAMANP+PEI PE+A +A + Sbjct: 320 NIRGTLADAIRGADVFIGVSAPDVLTVEHIESMADEPIVFAMANPDPEIRPEIAYGHARI 379 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPG+F+GAL+ RA+ IN++MKL V+ + L+ YII Sbjct: 380 IATGRSDYPNQINNVLCFPGVFRGALDIRAREINEEMKLAAARAIAGVIPEESLSEDYII 439 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+F+++VV V AV EA V SG+A+ Sbjct: 440 PSVFDERVVPAVAGAVAEAGVESGVAR 466 >gb|EAI09112.1| unknown [environmental sequence] Length = 466 Score = 416 bits (1068), Expect = e-115, Method: Compositional matrix adjust. Identities = 207/378 (54%), Positives = 261/378 (69%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 I+ +DR FLAH GKI LP+ DDL+ YTPGVA+VCT IH++PE Y LT+K Sbjct: 83 FINFSDRTFLAHLQGKIEINNRLPLSTRDDLSIAYTPGVARVCTEIHKNPENAYKLTIKG 142 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVVTDGTAVLGLG+IGP AA PVMEGKA+LFKQ A I+A+PICLDTTDTEEII VK Sbjct: 143 NTVAVVTDGTAVLGLGDIGPKAAMPVMEGKAMLFKQFADIDAWPICLDTTDTEEIISIVK 202 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 A+AP FGGINLEDIS+PRCF PVFHDDQHGTA+V A LINALK+ K+ Sbjct: 203 ALAPGFGGINLEDISAPRCFEIEERLRNELDIPVFHDDQHGTAVVTTAALINALKLVRKK 262 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 +++VV+G GAAG + +M++ G ++L+ D +GA+ + +W A + + Sbjct: 263 PKDIKVVVSGAGAAGTACSKMIMHLGVKNLIGCDSKGAIHNQRTDINQSKRWFADNANPD 322 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + G+L+EV+ GADVF+GVS IL D++ M IVFAMANP+PEI PE A P A V Sbjct: 323 QEQGSLKEVLKGADVFVGVSAPNILDAGDIRNMEKDAIVFAMANPDPEILPEDARPVAAV 382 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ I ++MKL + + LN YII Sbjct: 383 VATGRSDYPNQINNVLCFPGIFRGALDCRAKTITEEMKLAASHAIAETLDEQHLNPDYII 442 Query: 361 PSLFNDQVVIKVRQAVIE 378 P++F+ V K+ A+ E Sbjct: 443 PTVFDKSVTPKIAAAIAE 460 >ref|NP_863831.1| NAD-dependent malic enzyme [Pirellula sp. 1] emb|CAD71504.1| NAD-dependent malic enzyme [Pirellula sp.] Length = 476 Score = 413 bits (1061), Expect = e-114, Method: Compositional matrix adjust. Identities = 215/389 (55%), Positives = 276/389 (70%), Gaps = 3/389 (0%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 ++ +DR+FL H GGKI P +PI DDL+ YTPGVA+VC AI EDP+ +ALT+++ Sbjct: 79 VVHTSDRVFLMHLGGKIEVLPKMPIKTRDDLSMAYTPGVARVCNAIAEDPDSSFALTIRQ 138 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVV+DG+AVLGLGNIGP AA PVMEGKA+LFK+ AG++AFPICLDT DTE II TV+ Sbjct: 139 NTVAVVSDGSAVLGLGNIGPRAAMPVMEGKAMLFKEFAGVDAFPICLDTQDTEAIIETVR 198 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 +AP FGG+NLEDIS+PRC PVFHDDQHGTAIVVLAGL N+L GK Sbjct: 199 QLAPTFGGVNLEDISAPRCIEIEERLDKELEIPVFHDDQHGTAIVVLAGLKNSLLRVGKA 258 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHP--KWQWLAGQSG 238 + ++RIVVNG GAAG +I ++LL +GA+ +V D++GA I G + P QW+A + Sbjct: 259 LPNIRIVVNGAGAAGTAIVKLLLISGAKDIVVCDRDGA-IHAGEPSQPDASKQWIADNTN 317 Query: 239 VEDQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYA 298 G L EVI GADVFIGVS +LQ +DV MA PIVFA+ANP+PEI P A P+ Sbjct: 318 PRGASGKLSEVIQGADVFIGVSAPNVLQTEDVAAMAADPIVFALANPDPEILPANAAPHV 377 Query: 299 GVYATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAY 358 + ATGRSD+PNQINNVL FPG+F+G L+ RA IN++MKL + +++L Y Sbjct: 378 RIMATGRSDFPNQINNVLCFPGLFRGVLDVRATTINNEMKLAAAEAIAATIPESDLLDDY 437 Query: 359 IIPSLFNDQVVIKVRQAVIEAAVSSGIAK 387 +IPS+F+ +V V QAV +AAV +G+A+ Sbjct: 438 VIPSVFDRRVAKVVAQAVAKAAVDTGVAR 466 >ref|NP_627175.1| putative malate oxidoreductase [Streptomyces coelicolor A3(2)] emb|CAB72197.1| putative malate oxidoreductase [Streptomyces coelicolor A3(2)] Length = 471 Score = 408 bits (1049), Expect = e-113, Method: Compositional matrix adjust. Identities = 214/387 (55%), Positives = 270/387 (69%), Gaps = 5/387 (1%) Query: 4 ITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKRNMV 63 ++DR FL H GGKI + PI N DDL+ +YTPGVA+VC AI E+PE LT+KRN V Sbjct: 83 VSDRTFLMHLGGKIEMQSKHPIRNRDDLSMVYTPGVARVCMAIAENPEDARRLTIKRNSV 142 Query: 64 AVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVKAIA 123 AVVTDG+AVLGLGNIGP AA PVMEGKA LFK+ AGI+A+PICLDT DT+ I+ VKAIA Sbjct: 143 AVVTDGSAVLGLGNIGPKAALPVMEGKAALFKRFAGIDAWPICLDTQDTDAIVEIVKAIA 202 Query: 124 PIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQIDH 183 P F GINLEDIS+PRCF PVFHDDQHGTAIVVLA L NAL+V GK I++ Sbjct: 203 PGFAGINLEDISAPRCFEIEARLREALDIPVFHDDQHGTAIVVLAALTNALRVVGKGIEN 262 Query: 184 VRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGN---YAHPK--WQWLAGQSG 238 VR+V++G GAAG +I ++LLAAG ++ V D G + G A P+ +W+A + Sbjct: 263 VRVVMSGAGAAGTAILKLLLAAGVKNAVVADIHGVVHTGRADLVDAAPESALRWIADNTN 322 Query: 239 VEDQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYA 298 E GTL+E + GADVFIGVS +L +DV MA IVFA+ANP+PE+ P +A A Sbjct: 323 PEGLTGTLKEAVHGADVFIGVSAPNVLDGEDVAAMADGAIVFALANPDPEVDPAIARETA 382 Query: 299 GVYATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAY 358 V ATGRSD+PNQINNVL FPG+F+G L+ +++ +N +M L VV + E+N Y Sbjct: 383 AVVATGRSDFPNQINNVLVFPGVFRGLLDAQSRTVNTEMMLAAAHALANVVTEDEINPNY 442 Query: 359 IIPSLFNDQVVIKVRQAVIEAAVSSGI 385 IIPS+FND+V V AV +AA ++G+ Sbjct: 443 IIPSVFNDKVAGAVAGAVRDAAKAAGV 469 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.140 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 555,607,159 Number of extensions: 22231047 Number of successful extensions: 53154 Number of sequences better than 1.0e-05: 821 Number of HSP's gapped: 51840 Number of HSP's successfully gapped: 821 Length of query: 395 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 267 Effective length of database: 409,704,906 Effective search space: 109391209902 Effective search space used: 109391209902 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)