BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0102.p (400 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391713.1| alternate gene name: ipa-21r~similar to sugar p... 146 9e-34 sp|Q45632|MALA_BACST Maltose permease >gi|1075815|pir||S43915 ma... 122 1e-26 ref|NP_243142.1| BH2276~unknown conserved protein [Bacillus halo... 116 1e-24 ref|NP_691645.1| hypothetical protein OB0724 [Oceanobacillus ihe... 115 1e-24 ref|NP_624025.1| Permeases of the major facilitator superfamily ... 102 2e-20 >ref|NP_391713.1| alternate gene name: ipa-21r~similar to sugar permease [Bacillus subtilis] sp|P39589|YWBF_BACSU Hypothetical transport protein ywbF pir||S39676 sugar permease homolog ywbF - Bacillus subtilis emb|CAA51577.1| ipa-21r [Bacillus subtilis] emb|CAB15860.1| ywbF [Bacillus subtilis subsp. subtilis str. 168] Length = 399 Score = 146 bits (368), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 108/383 (28%), Positives = 180/383 (46%), Gaps = 19/383 (4%) Query: 6 RKYS-LHVYNYFFYIAYASYLPFIS-YWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGML 63 R Y+ L + F ++ S +P +S Y E LS Q+GLI S+GP+V QP WGML Sbjct: 8 RHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGML 67 Query: 64 IDYYGMAKWLLLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRH 123 DY K LL + T IT + +AY A F L+I+I+ A F S +P+ D++++R+ Sbjct: 68 SDYTQKTKGLLAVCTSITGIIGLAY-IAFDSFPLFILIAACFAAFQSTIIPLSDSISLRY 126 Query: 124 AKHNSLSYGAIRVVGSISFGLAVAVFGQMYEH-FGISTVFAAYILTMTLVCLLTFSIPGD 182 + + +YG IR+ GS+ FG+AV GQ+ + I +F + + +L +PG Sbjct: 127 TQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLYPIHVIFIFGCAFLCIAAILASQVPGQ 186 Query: 183 RKGRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHF 242 +K + + G L+ F+ F+I F NN ++S+++ Sbjct: 187 QK-----------TTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKS 235 Query: 243 GGEASAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFE 302 G SA IG+L+ ++EIP F+ I K G + ++ RW + PS Sbjct: 236 GASLSA-IGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLW 294 Query: 303 ILMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVV 362 I+ A L G+ LF+ A + Y + + TA +++ + + GG++ Sbjct: 295 IIYATVFLQGVAIGLFIPAALQYVKKITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIF 354 Query: 363 ERGGYSLLFQGAAVMSLLGAAGF 385 + Y +F + +L AGF Sbjct: 355 D---YVSIFAVYLLFGILSIAGF 374 >sp|Q45632|MALA_BACST Maltose permease pir||S43915 maltose permease - Bacillus stearothermophilus gb|AAA71980.1| maltose permease Length = 394 Score = 122 bits (307), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 106/396 (26%), Positives = 190/396 (47%), Gaps = 15/396 (3%) Query: 6 RKYSLHVYNYFFYIAYASYLPFISYWFAEDG-LSTQQIGLIFSIGPLVGFLVQPLWGMLI 64 RK ++ + + A+ + P +S + E+ LS IG I S+ P+V QPLWG Sbjct: 12 RKTVFPLFYFLIFFAFGALFPLLSVYLQEEARLSGAAIGWIMSLPPIVTMAAQPLWGTAA 71 Query: 65 DYYGMAKWLLLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHA 124 DY LLL + + + Y AG + L++V++V+L+ SA +P+ D++ +RH Sbjct: 72 DYTRKPVGLLLAALVLAALFGVMYALAGSYR-LFVVLTVLLSAMQSAIVPLSDSLALRHV 130 Query: 125 KHNSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRK 184 +YGAIR+ GS+ F +AV G + +H + +F A+ L + L +P Sbjct: 131 HEQGGNYGAIRLWGSLGFAMAVLAVGWLSDHIAFAVIFYAFSLALLTAAALATRLP---- 186 Query: 185 GRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQM-NNAFYSVYISHFG 243 + M GAP + + LL F L+ FL GP + NN+++ + I G Sbjct: 187 ---RYPM---GAPGALTRQDVRGLLASRPFRLLLVATFLL-FGPILANNSYFGLLIHELG 239 Query: 244 GEASAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEI 303 G + IGL + A+ +E P + +I +FG V++L +L A RW + +P Sbjct: 240 GTLTG-IGLAFLFAAGSEAPFMKAADRLIGRFGMVRLLLLAALISAARWLAYAADPPLWF 298 Query: 304 LMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVE 363 + ++ G + L + + YA + +R ++TA +L+ V + + VGG+++E Sbjct: 299 VYMTTVVQGCSVGLAIPTALQYARRLAPERVQSTAVALYSAVGNGLGAWFCTLVGGYLLE 358 Query: 364 RGGYSLLFQGAAVMSLLGAAGFASLGRVKRREGRLK 399 R ++ ++ +++G L + +R G K Sbjct: 359 RWQIGAVYLFFSICTIVGVLVLLLLAKRERTAGEEK 394 >ref|NP_243142.1| BH2276~unknown conserved protein [Bacillus halodurans] pir||D83934 hypothetical protein BH2276 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05995.1| BH2276~unknown conserved protein [Bacillus halodurans] Length = 392 Score = 116 bits (290), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 97/378 (25%), Positives = 175/378 (46%), Gaps = 15/378 (3%) Query: 8 YSLHVYNYFFYIAYASYLPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYY 67 Y L Y +F Y + ++ +F+ GL+ +IG++ ++GP L QP WG L D Y Sbjct: 8 YRLMAYLFFAYSSMTIVTSYLPVYFSAVGLTAGEIGVLMAVGPFAMILAQPTWGFLSDKY 67 Query: 68 GMAKWLLLISTGITPWVVIAYRFAGH-HFWLYIVISVVLAVFSSATLPVIDAVTVRHAKH 126 K +L I+ + +V + F G F Y ++ VL +F S T + D++ + A + Sbjct: 68 KSIKRMLQIA--LIGMIVAIFAFMGAASFAGYFIMMFVLFLFLSPTTALGDSLAQKTAIN 125 Query: 127 NSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRKGR 186 LS+G IR+ GS+ FG G + G++ + ++ + L+F + Sbjct: 126 RRLSFGKIRMWGSLGFGFTSLAVGYILAAIGVTYIMVPLLVVTVISLWLSFGLE------ 179 Query: 187 PKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHFGGEA 246 + + P + ++A + L +PK +FL+ + + + N+ F S+YI GG Sbjct: 180 ---DFSDTTKPVTLLSA--LKLAIDPKIFFFLVCIVFITVTHRTNDIFLSIYIVELGGPE 234 Query: 247 SAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEILMA 306 S IG + TE V S RKF + + ++ +RW+++S+ +L+ Sbjct: 235 S-YIGWAWFIGVATEAAVLATSTLWFRKFSPIGFVIFAAVFYGVRWWLVSMVAVPWLLLP 293 Query: 307 NQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVERGG 366 Q L G+T+ +F A + + T H LF+ ++GI S GGW+++ Sbjct: 294 LQTLHGMTFGVFYVAAFAFVSHLIPKHLQATGHVLFISTTFGLSGIFGSLFGGWMIQAFS 353 Query: 367 YSLLFQGAAVMSLLGAAG 384 L+ A+ + +GA G Sbjct: 354 IPTLYSYLALSAFIGALG 371 >ref|NP_691645.1| hypothetical protein OB0724 [Oceanobacillus iheyensis HTE831] dbj|BAC12680.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 390 Score = 115 bits (289), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 93/359 (25%), Positives = 166/359 (46%), Gaps = 15/359 (4%) Query: 25 LPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYYGMAKWLLLIS-TGITPW 83 + F+ + GLS QIG + ++ L L QP WG L D + K +L I G+ + Sbjct: 25 ISFLPLYLQSRGLSGTQIGWVLAVESLAAILAQPFWGFLSDKFKTIKRILFICLIGLLIF 84 Query: 84 VVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHAKHNSLSYGAIRVVGSISFG 143 ++ + H ++ V F++ + D+ R A +S+G+IR+ GS+ F Sbjct: 85 GFTFFQMSSLH--TILIFGAVFYFFAAPIGGLNDSFAQRRAGQLGISFGSIRMWGSVGFA 142 Query: 144 LAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRKGRPKPEMAGKGAPRSGMTA 203 L+ + G++ FGI + Y L + LLT D K KP Sbjct: 143 LSSLLVGEVLARFGIGYMVWVY-LAFGTIALLTLIPLKDVKADTKPVKLND--------- 192 Query: 204 GMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHFGGEASAKIGLLYTAASLTEIP 263 + ++K F+ FL + I +MN+ + ++YIS GG +GL + A ++E Sbjct: 193 -ISKIIKSKPFLLFLGVILFITITHRMNDYYMALYISELGGSEDL-VGLAWFAGVISEAA 250 Query: 264 VFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEILMANQMLSGLTYALFLSAGV 323 VF + + RKF + + ++ LRW++ + +++ Q L GLT+ +F +A Sbjct: 251 VFALAAFWFRKFHSLIFIIIAAIIYTLRWFLYATATDPMMIIGLQFLHGLTFGVFYTAAF 310 Query: 324 NYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVERGGYSLLFQGAAVMSLLGA 382 +Y ++T H +F VY ++GI+ S GG +V+ G S ++ ++S +G Sbjct: 311 DYVTRLIPSFLQSTGHLVFFSVYFGLSGIIGSLAGGSLVDVFGGSSMYTLMGILSAIGT 369 >ref|NP_624025.1| Permeases of the major facilitator superfamily [Thermoanaerobacter tengcongensis] gb|AAM25629.1| Permeases of the major facilitator superfamily [Thermoanaerobacter tengcongensis] Length = 382 Score = 102 bits (253), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 79/365 (21%), Positives = 175/365 (47%), Gaps = 20/365 (5%) Query: 17 FYIAYASYLPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYYGMAKWLLLI 76 F++ A Y F+ + G S +IG++ S+GP V + QP WG+ D ++L I Sbjct: 18 FFMGGAIYGTFVPVYLDYIGYSKSEIGILMSLGPFVAIIAQPFWGITSDRARSKNFVLQI 77 Query: 77 STGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHAKHNSLSYGAIRV 136 + +++ + + + ++L VI++ F ++T P+ DA+T+ + S+G IR+ Sbjct: 78 LFLASIFIMALFPLSRNFYYLVAVITL-FTFFQTSTGPISDAITLEYLSGTGQSFGPIRM 136 Query: 137 VGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIP---GDRKGRPKPEMAG 193 G+I + + + G + I ++F I+ M + ++ + +P G + G+ K + Sbjct: 137 AGTIGYAVMSVIAGWFAQR-HIGSIFILNIIVMFIAFMIVYKLPVVKGHQFGKEKISIWK 195 Query: 194 KGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHFGGEASAKIGLL 253 + R+ + ++ L + ++ ++ +Y GG ++ +GL Sbjct: 196 LFSNRNLVVLMLLNFLIQTTLGFYY--------------TYFPIYFRKIGG-SNELLGLA 240 Query: 254 YTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEILMANQMLSGL 313 Y ++++EIP L++ I+++ G L S ALRW ++S P+ +++ Q+L GL Sbjct: 241 YFISAMSEIPFLLYADKILKRIGTRWALIGASGVAALRWLIISFIPNAYLVLPFQLLHGL 300 Query: 314 TYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVERGGYSLLFQG 373 + + + Y K + ++ ++ ++ IV S +GG++ + G ++F Sbjct: 301 IFIVLYYSMATYINQEVPKELKASGQTVNNLIGMGISRIVGSLLGGFLSDLYGIKMVFLY 360 Query: 374 AAVMS 378 +V++ Sbjct: 361 NSVLA 365 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.141 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 578,668,848 Number of extensions: 24319172 Number of successful extensions: 88790 Number of sequences better than 1.0e-05: 125 Number of HSP's gapped: 88730 Number of HSP's successfully gapped: 125 Length of query: 400 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 272 Effective length of database: 409,704,906 Effective search space: 111439734432 Effective search space used: 111439734432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)