BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0084.p (181 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242572.1| BH1706~unknown conserved protein in B. subtilis... 71 9e-12 ref|NP_391584.1| ywkC [Bacillus subtilis] >gi|1176958|sp|P45870|... 68 1e-10 >ref|NP_242572.1| BH1706~unknown conserved protein in B. subtilis [Bacillus halodurans] pir||B83863 hypothetical protein BH1706 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05425.1| BH1706~unknown conserved protein in B. subtilis [Bacillus halodurans] Length = 174 Score = 71.2 bits (173), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 49/178 (27%), Positives = 96/178 (53%), Gaps = 13/178 (7%) Query: 5 KTKDAAELLSVSQTTIKRWAAMFPDYFPKDRFGHYIFSEQEISLLKSIKERINHGEVLEY 64 KTK+ +E L V+ TT++RW F + GH +F ++ +++LK+I+ ++ G+ + Sbjct: 6 KTKEVSEQLGVNPTTVQRWVKFFGIQCETNEHGHLLFEKEHLTVLKAIQNQLKSGKKMRD 65 Query: 65 MNLGSAAG-IQPPVSQQENPLHHTQDWP--MGDMLSRIDYIERSLDHKADEVVSVQLLQQ 121 + + IQ P +Q P+ T+ + + +I +E+ L KAD+VVS Q+L+ Sbjct: 66 VVIEENEQLIQKPETQ---PMVATEQYEQRTKQFMEKIAELEKQLSQKADDVVSYQILKH 122 Query: 122 RAELEDLRQMIKQLAVSLETIQKPGAPSLSSHDELHPVAAKKLKTPPRKRGLLRNIFS 179 RAE++++ + ++ L + + +++ P+AA + P RKR +R +FS Sbjct: 123 RAEIDEMFKTLQSLELRMAKVEEQLEKQQEE----FPLAAGDV--PKRKRSFMR-LFS 173 >ref|NP_391584.1| ywkC [Bacillus subtilis] sp|P45870|YWKC_BACSU Hypothetical protein ywkC pir||S55435 hypothetical protein ywkC - Bacillus subtilis emb|CAA89882.1| Unknown [Bacillus subtilis] emb|CAB15720.1| ywkC [Bacillus subtilis subsp. subtilis str. 168] Length = 184 Score = 67.8 bits (164), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 50/186 (26%), Positives = 86/186 (46%), Gaps = 24/186 (12%) Query: 4 LKTKDAAELLSVSQTTIKRWAAMFPDYFPKDRFGHYIFSEQEISLLKSIKERINHGEVLE 63 + T A L VS T++RW ++ GHY F+ +++ +LKS++++I+ G ++ Sbjct: 1 MNTNMVASELGVSAKTVQRWVKQLNLPAERNELGHYSFTAEDVKVLKSVQKQISEGTAIQ 60 Query: 64 YMNLGSAAG------IQPPVSQQENPLHH---------TQDWPMGDMLSRIDYIERSLDH 108 ++L +A +Q S E + Q ++++R+ +ER L Sbjct: 61 DIHLPKSAKKRTGFLVQKTSSDTERRIEQLEQKLDTLLQQRQDESELMARMSELERQLKQ 120 Query: 109 KADEVVSVQLLQQRAELEDLRQMIKQLAVSLETIQKPGAPSLSSHDELHPVAAKKLKTPP 168 KADE VS QLLQ R E++D+ ++ L ++ P AA KT Sbjct: 121 KADEGVSYQLLQHRREIDDILADLQSLTSQMKEFTAHSIP---------ETAAASEKTKT 171 Query: 169 RKRGLL 174 RK+ LL Sbjct: 172 RKKPLL 177 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 252,975,824 Number of extensions: 9275566 Number of successful extensions: 28915 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 28912 Number of HSP's successfully gapped: 3 Length of query: 181 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 63 Effective length of database: 436,855,896 Effective search space: 27521921448 Effective search space used: 27521921448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)