BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0074.p (322 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00021081.1| hypothetical protein [Chloroflexus aurantiacus] 199 5e-50 ref|NP_228671.1| glucose-1-phosphate thymidylyltransferase [Ther... 192 1e-47 ref|NP_111418.1| dTDP-glucose pyrophosphorylase [Thermoplasma vo... 180 4e-44 dbj|BAB60061.1| glucose-1-phosphate thymidylyltransferase [Therm... 180 4e-44 dbj|BAC55207.1| glucose-1-phosphate thymidyltransferase [Strepto... 176 7e-43 >ref|ZP_00021081.1| hypothetical protein [Chloroflexus aurantiacus] Length = 355 Score = 199 bits (507), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 120/333 (36%), Positives = 186/333 (55%), Gaps = 28/333 (8%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVNHTQGEIRRTIGDGERWNASLTFLMQEEPMG 60 ++PV + +L +I + AGIT+IGIV+ T EIR +G+G RW ++++ QE P+G Sbjct: 25 LVPVANKPVLFRVIEAIRDAGITDIGIVIGDTGDEIRSAVGNGRRWGVKISYIPQEAPLG 84 Query: 61 LADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGVA 120 LA AVK ++ F+ D FV+ LGDN +G I+PLI + A I+L++V DP FGVA Sbjct: 85 LAHAVKISRDFLGDDRFVMFLGDNCIQGGISPLIEQFGRSDYNAQIVLKKVSDPRSFGVA 144 Query: 121 AV-HAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 + G+++ L EKP+ PKSDLA+VG+Y I+ A++ + PS RGE E+TDAIQ L++ Sbjct: 145 ELDDEGRVVRLVEKPREPKSDLALVGIYMFDHHIFEAVEAIRPSARGELEITDAIQWLVS 204 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQ-DP--QNFVKPSGMHSSVQWIPPVRID 236 G +V + + D G D LEANR + + +P Q +V Q I V I+ Sbjct: 205 NGYNVYPYVHEGWWIDTGKKDDMLEANRLVLEELEPSVQGYVD-----RDSQLIGKVIIE 259 Query: 237 PTATVMNSV-----------------VGPYVYIGPNSVVEDCILSNSILTDQVTLSHV-- 277 P A ++NS VGP+ I + +E+C + +SI+ + T+ + Sbjct: 260 PGAEIINSTIRGPAIIGEHTRIVNAYVGPFTSIYHHCHIEECEIEHSIVLEYCTIRGLPH 319 Query: 278 HAEGSVFGSYVHLAEQEIQEQPARHILGDRASV 310 E S+ G V + ++ + R +LGD +SV Sbjct: 320 RLEDSLIGRNVEIGRSPLRPKAYRFMLGDNSSV 352 >ref|NP_228671.1| glucose-1-phosphate thymidylyltransferase [Thermotoga maritima] pir||G72325 glucose-1-phosphate thymidylyltransferase - Thermotoga maritima (strain MSB8) gb|AAD35944.1| glucose-1-phosphate thymidylyltransferase [Thermotoga maritima] Length = 355 Score = 192 bits (488), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 116/330 (35%), Positives = 179/330 (54%), Gaps = 22/330 (6%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVN-HTQGEIRRTIGDGERWNASLTFLMQEEPM 59 ++P+ + IL + + AGI E+GIVV+ H E + +G GE + +++++QEEP Sbjct: 26 LIPIANKPILFYSLENIARAGIEEVGIVVSPHNAEEFKSIVGTGENFGLRISYIIQEEPK 85 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 GLA AV ++ F+ D F++ LGDNL D+ ++ E + AASILL VKDP +FGV Sbjct: 86 GLAHAVWVSREFLGDEDFMMYLGDNLILEDLGKFVKDFENSDYAASILLSPVKDPTRFGV 145 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A + ++I + EKPK P S+LAIVG+Y I+ + + PS RGE E+TDAI+ LI Sbjct: 146 AVMEGDRVIKVVEKPKTPPSNLAIVGLYLFRNKIFEGIKNIKPSWRGELEITDAIEYLIE 205 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQDPQNFVKPSGMHSSVQWIPPVRIDPTA 239 +G V I + D G + LEANR + + + + SS+Q PVRI + Sbjct: 206 KGEKVRGYIVYGWWKDTGKPEDLLEANRKILMETTEELLGEMDDKSSIQ--GPVRIGKAS 263 Query: 240 TVMNSV-----------------VGPYVYIGPNSVVEDCILSNSILTDQVTLSHVHA--E 280 ++NSV VGPY IG ++EDC + NSI+ D+ +++ V + Sbjct: 264 KIVNSVIRGPAVIGENCFIKNSYVGPYSSIGNRVILEDCEVENSIVMDECSITGVEKRID 323 Query: 281 GSVFGSYVHLAEQEIQEQPARHILGDRASV 310 S+ G V + + + ILG+ + V Sbjct: 324 SSILGKGVSVKGSQKRPASLNLILGNMSRV 353 >ref|NP_111418.1| dTDP-glucose pyrophosphorylase [Thermoplasma volcanium] Length = 349 Score = 180 bits (457), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 103/282 (36%), Positives = 158/282 (56%), Gaps = 12/282 (4%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVNHTQG-EIRRTIGDGERWNASLTFLMQEEPM 59 +LP+ G+ I + L+ GI I IV+ G E+++ GDG RWN ++++ Q EP+ Sbjct: 25 LLPIAGKPISEYALENLIEIGIKNINIVIGSVGGLEVKKFYGDGSRWNVNISYTYQPEPL 84 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 G+A A+ K+F+ + FV+ LGDN + I+ L + + L V +P QFG+ Sbjct: 85 GIAHAIGLTKAFVGNDDFVVFLGDNYLQNGISNLYEDFTNAGSDGHLGLVPVDNPSQFGI 144 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A V G+I L EKPK P S+LAIVGVY LT ++ ++DRL PS RGEYE+T+A Q +I Sbjct: 145 AEVDNGKISKLVEKPKTPTSNLAIVGVYFLTPKVFESIDRLKPSKRGEYEITEAYQDMID 204 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRY---KMGQDPQ--NFVKPSGMHSSVQW----- 229 G + YSI + F D GT D +L NR K+G + + N +H +V+ Sbjct: 205 RGLKISYSIISGWFKDTGTVDDFLACNRLILDKLGDNGRRDNVSGRFDIHPTVKISSDSE 264 Query: 230 -IPPVRIDPTATVMNSVVGPYVYIGPNSVVEDCILSNSILTD 270 + P I + +S +GPY IG N ++++ + +SI+ D Sbjct: 265 VVGPCFIGEGTRIEHSYIGPYTSIGSNCIIKNAEIEDSIIMD 306 >dbj|BAB60061.1| glucose-1-phosphate thymidylyltransferase [Thermoplasma volcanium] Length = 351 Score = 180 bits (456), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 103/282 (36%), Positives = 158/282 (56%), Gaps = 12/282 (4%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVNHTQG-EIRRTIGDGERWNASLTFLMQEEPM 59 +LP+ G+ I + L+ GI I IV+ G E+++ GDG RWN ++++ Q EP+ Sbjct: 27 LLPIAGKPISEYALENLIEIGIKNINIVIGSVGGLEVKKFYGDGSRWNVNISYTYQPEPL 86 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 G+A A+ K+F+ + FV+ LGDN + I+ L + + L V +P QFG+ Sbjct: 87 GIAHAIGLTKAFVGNDDFVVFLGDNYLQNGISNLYEDFTNAGSDGHLGLVPVDNPSQFGI 146 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A V G+I L EKPK P S+LAIVGVY LT ++ ++DRL PS RGEYE+T+A Q +I Sbjct: 147 AEVDNGKISKLVEKPKTPTSNLAIVGVYFLTPKVFESIDRLKPSKRGEYEITEAYQDMID 206 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRY---KMGQDPQ--NFVKPSGMHSSVQW----- 229 G + YSI + F D GT D +L NR K+G + + N +H +V+ Sbjct: 207 RGLKISYSIISGWFKDTGTVDDFLACNRLILDKLGDNGRRDNVSGRFDIHPTVKISSDSE 266 Query: 230 -IPPVRIDPTATVMNSVVGPYVYIGPNSVVEDCILSNSILTD 270 + P I + +S +GPY IG N ++++ + +SI+ D Sbjct: 267 VVGPCFIGEGTRIEHSYIGPYTSIGSNCIIKNAEIEDSIIMD 308 >dbj|BAC55207.1| glucose-1-phosphate thymidyltransferase [Streptomyces sp. TP-A0274] Length = 350 Score = 176 bits (446), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 120/329 (36%), Positives = 170/329 (51%), Gaps = 22/329 (6%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVNHTQGEIRRTIGDGERWNASLTFLMQEEPMG 60 ++PV + +L + + AGI E+GIVV T EI +GDG R+ +T+L QE P+G Sbjct: 21 LVPVANKPVLFYGLESIAEAGIREVGIVVGDTAAEIEAAVGDGSRFGLDVTYLPQEAPLG 80 Query: 61 LADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGVA 120 LA AV A+ ++ D FV+ LGDN G I L+ A ILL +V DP FGV Sbjct: 81 LAHAVTIARDYLGDDDFVMYLGDNFIVGGIGALLDRFRVSRPDAQILLTRVSDPRAFGVV 140 Query: 121 AVHA-GQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 + G++IGLEEKP++P+SDLA+VGVY +A + A+ RL PS RGE E+TDAIQ LI Sbjct: 141 ELDTDGRVIGLEEKPEHPRSDLALVGVYMFSARVHDAVSRLKPSWRGELEITDAIQELID 200 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQDPQNFVKPSGMHSSVQWIPPVRIDPTA 239 V ++ + + D G LE NR + + SG+ S + + V I+ A Sbjct: 201 ADCRVESTLVSGYWKDTGNVADMLEVNRLVLDGVEADC---SGVVDSSELVGRVVIEEGA 257 Query: 240 TVMNS-VVGPYV----------YIGP-NSVVEDCILSNSILTDQVTLSHV------HAEG 281 TV S +VGP V YIGP S+ C + +S + + L E Sbjct: 258 TVTGSRIVGPVVVAAGSRIHDSYIGPFTSIDRGCSVIDSEVEYSIVLRGASIVGVRRVER 317 Query: 282 SVFGSYVHLAEQEIQEQPARHILGDRASV 310 S+ G V + + R +LGD + V Sbjct: 318 SLIGREVEVTSTPRILRSHRLVLGDHSKV 346 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 470,836,820 Number of extensions: 19131879 Number of successful extensions: 45265 Number of sequences better than 1.0e-05: 1033 Number of HSP's gapped: 44146 Number of HSP's successfully gapped: 1033 Length of query: 322 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 196 Effective length of database: 415,135,104 Effective search space: 81366480384 Effective search space used: 81366480384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)