BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0072.p (967 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella th... 114 2e-23 sp|P38537|SLAP_BACSH SURFACE-LAYER 125 KD PROTEIN PRECURSOR >gi|... 110 2e-22 ref|ZP_00061713.1| hypothetical protein [Clostridium thermocellu... 109 4e-22 gb|AAD09354.1| s-layer associated multidomain endoglucanase [The... 106 4e-21 gb|AAF22978.1| surface layer protein precursor [Bacillus sphaeri... 105 6e-21 >ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella thermoacetica ATCC 39073] Length = 368 Score = 114 bits (284), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 63/170 (37%), Positives = 98/170 (57%), Gaps = 8/170 (4%) Query: 42 YAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLSLGLKVNGDQAAPFKDVDSK 101 +AQ I++LA AGI++G + + F+P +A++RAE A ++V +LGL+ + PFKDV Sbjct: 202 WAQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASLLVKALGLEGKAGEKNPFKDVKGD 261 Query: 102 SWYRGYVGALVESGITEGTSATTFSPNSNVTREELVVFFIRAFGLEDTAKKLPVDSKLSD 161 +WY G V A +G+ +G TF P++ +TREEL +RA L +KL D Sbjct: 262 AWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELAAMLVRAVNLPAGEEKL----AFKD 317 Query: 162 ILEVSEWAKAQVSLAFKNGFINGVQGSDGTLKFKPKEHAERQALARLAYE 211 +VS WA+ V++A +G + G + DGT F+P + A R A + Y Sbjct: 318 NDKVSTWARKSVAVAAAHGLVKGFE--DGT--FRPGDAASRAECAVMVYR 363 Score = 66.6 bits (161), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 42/130 (32%), Positives = 63/130 (48%), Gaps = 3/130 (2%) Query: 17 ALLVSTLMPHMVFGEAAQFKDIAG-SYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAEL 75 +LLV L GE FKD+ G ++ + + A G++ GYED F+P+ ++R EL Sbjct: 237 SLLVKALGLEGKAGEKNPFKDVKGDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREEL 296 Query: 76 AKIIVLSLGLKVNGDQAAPFKDVDSKS-WYRGYVGALVESGITEGTSATTFSPNSNVTRE 134 A ++V ++ L G++ FKD D S W R V G+ +G TF P +R Sbjct: 297 AAMLVRAVNLPA-GEEKLAFKDNDKVSTWARKSVAVAAAHGLVKGFEDGTFRPGDAASRA 355 Query: 135 ELVVFFIRAF 144 E V R Sbjct: 356 ECAVMVYRVL 365 >sp|P38537|SLAP_BACSH SURFACE-LAYER 125 KD PROTEIN PRECURSOR pir||A33856 surface-layer 125K protein precursor - Bacillus sphaericus gb|AAA50256.1| surface layer protein Length = 1176 Score = 110 bits (274), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 68/184 (36%), Positives = 96/184 (52%), Gaps = 8/184 (4%) Query: 23 LMPHMVFGEAAQFKDIAGSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLS 82 ++P + F I+G YA++ +QSL +AG+I G + F P K +SRAE A I + Sbjct: 24 IVPVASAAQLNDFNKISG-YAKEAVQSLVDAGVIQGDANGNFNPLKTISRAEAATIFTNA 82 Query: 83 LGLKVNGDQAAPFKDVDSKSWYRGYVGALVESGITEGTSATTFSPNSNVTREELVVFFIR 142 L L+ GD FKDV + +WY + A VE+GI EG SAT F+PN +TR E + Sbjct: 83 LELEAEGD--VNFKDVKADAWYYDAIAATVENGIFEGVSATEFAPNKQLTRSEAAKILVD 140 Query: 143 AFGLEDTAKKLPVDSKLSDILEVSEWAKAQVSLAFKNGFINGVQGSDGTLKFKPKEHAER 202 AF LE S+ +D V WAK+ + +A NG I G + ++G P R Sbjct: 141 AFELEGEGDL----SEFADASTVKPWAKSYLEIAVANGVIKGSE-ANGKTNLNPNAPITR 195 Query: 203 QALA 206 Q A Sbjct: 196 QDFA 199 Score = 64.3 bits (155), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 48/151 (31%), Positives = 78/151 (51%), Gaps = 11/151 (7%) Query: 34 QFKDI-AGSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLSLGLKVNGDQA 92 FKD+ A ++ I + E GI G F PNK ++R+E AKI+V + L+ GD Sbjct: 92 NFKDVKADAWYYDAIAATVENGIFEGVSATEFAPNKQLTRSEAAKILVDAFELEGEGD-L 150 Query: 93 APFKDVDS-KSWYRGYVGALVESGITEGTSA---TTFSPNSNVTREELVVFFIRAFGLED 148 + F D + K W + Y+ V +G+ +G+ A T +PN+ +TR++ V F R D Sbjct: 151 SEFADASTVKPWAKSYLEIAVANGVIKGSEANGKTNLNPNAPITRQDFAVVFSRTIENVD 210 Query: 149 TAKKLP----VDSKLSDILEVSEWAKAQVSL 175 K+ VD+K ++ +S+ K V+L Sbjct: 211 ATPKVDKIEVVDAKTLNVT-LSDGTKETVTL 240 >ref|ZP_00061713.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 710 Score = 109 bits (272), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 70/172 (40%), Positives = 100/172 (58%), Gaps = 8/172 (4%) Query: 32 AAQFKDIAGSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLSLGLKVNGDQ 91 AA F DIAG +A++ I LA ++SGY D + KP+ ++RAE+A I+V S GL+ + Sbjct: 518 AAPFNDIAGHWAEEFIAKLAARNVVSGYPDGSVKPDIEITRAEMAVIVVKSAGLEPVENV 577 Query: 92 AAPFKDVDS-KSWYRGYVGALVESGITEGTSATTFSPNSNVTREELVVFFIRAFGLEDTA 150 + FKD D +W GYV A VE+GI G TF P+ N+TREE+VV ++A+ E A Sbjct: 578 SLKFKDADQIPAWAAGYVQAGVEAGIIAGYEDNTFRPSRNLTREEMVVLIMKAY--EFGA 635 Query: 151 KKLPVDSKLSDILEVSEWAKAQVSLAFKNGFINGVQGSDGTLKFKPKEHAER 202 + P + D E+ +W+K V A + GF+ G D T FKPK+ R Sbjct: 636 VENP-EFSFIDASEIGDWSKPFVGKAVELGFVVGY--PDNT--FKPKKSVTR 682 Score = 62.4 bits (150), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 37/108 (34%), Positives = 55/108 (50%), Gaps = 5/108 (4%) Query: 29 FGEAAQFKDIAGSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLSLGLKVN 88 F +A Q A Y +Q+ EAGII+GYEDN F+P++ ++R E+ +I+ + Sbjct: 581 FKDADQIPAWAAGY----VQAGVEAGIIAGYEDNTFRPSRNLTREEMVVLIMKAYEFGAV 636 Query: 89 GDQAAPFKDV-DSKSWYRGYVGALVESGITEGTSATTFSPNSNVTREE 135 + F D + W + +VG VE G G TF P +VTR E Sbjct: 637 ENPEFSFIDASEIGDWSKPFVGKAVELGFVVGYPDNTFKPKKSVTRAE 684 >gb|AAD09354.1| s-layer associated multidomain endoglucanase [Thermoanaerobacterium polysaccharolyticum] Length = 1097 Score = 106 bits (264), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 64/186 (34%), Positives = 91/186 (48%), Gaps = 10/186 (5%) Query: 32 AAQFKDIAGSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLSLGLKVNGDQ 91 A +F DI+ S+A+ EIQ LA IISGY D FKP+K ++RAE ++V +LG+K + Sbjct: 910 APKFTDISSSWAKNEIQVLASKNIISGYPDGTFKPDKRITRAEFVSMLVKALGIKQSIPD 969 Query: 92 AAPFKDVDSKSWYRGYVGALVESGITEGTSATTFSPNSNVTREELVVFFIRAFGLEDTAK 151 F DV+ WY G V A +GI G F P+ +TR+E++V + A + K Sbjct: 970 VPTFSDVNKGDWYYGLVEAAKSTGIASGY-GKQFKPDMQITRQEMMVMVVNALRVNKVEK 1028 Query: 152 -----KLPVDSKLSDILEVSEWAKAQVSLAFKNGFINGVQGSDGTLKFKPKEHAERQALA 206 + V K D +V WAK +++ NG I G G P A R A Sbjct: 1029 FVSKGDVSVLGKFKDGGKVQNWAKDAMAIGVSNGLIKGT----GDEYLSPDGRATRAQAA 1084 Query: 207 RLAYEF 212 + Y Sbjct: 1085 AVIYRM 1090 >gb|AAF22978.1| surface layer protein precursor [Bacillus sphaericus] Length = 1268 Score = 105 bits (262), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 62/184 (33%), Positives = 96/184 (52%), Gaps = 8/184 (4%) Query: 23 LMPHMVFGEAAQFKDIAGSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLS 82 ++P + + I+G YA++ +QSL + G+I G + F P ++RA+ A+I + Sbjct: 24 IVPVASAAQVNDYNKISG-YAKEAVQSLVDQGVIQGDTNGNFNPLNTVTRAQAAEIFTKA 82 Query: 83 LGLKVNGDQAAPFKDVDSKSWYRGYVGALVESGITEGTSATTFSPNSNVTREELVVFFIR 142 L L+ +GD F DV +WY + A+V +GI EG SA F+PN ++TR E + Sbjct: 83 LELEADGD--VNFSDVKKGAWYYNSIAAVVANGIFEGVSANEFAPNKSLTRSEAAKVLVD 140 Query: 143 AFGLEDTAKKLPVDSKLSDILEVSEWAKAQVSLAFKNGFINGVQGSDGTLKFKPKEHAER 202 AFGLE + S+ +D +V WAK+ + A NG G + +G L KP R Sbjct: 141 AFGLEGSESL----SQFADASQVKGWAKSALETAVANGIFTGSE-ENGKLNLKPNAAITR 195 Query: 203 QALA 206 Q A Sbjct: 196 QDFA 199 Score = 57.0 bits (136), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 35/118 (29%), Positives = 58/118 (49%), Gaps = 6/118 (5%) Query: 34 QFKDIA-GSYAQKEIQSLAEAGIISGYEDNAFKPNKAMSRAELAKIIVLSLGLKVNGDQA 92 F D+ G++ I ++ GI G N F PNK+++R+E AK++V + GL+ + Sbjct: 92 NFSDVKKGAWYYNSIAAVVANGIFEGVSANEFAPNKSLTRSEAAKVLVDAFGLE-GSESL 150 Query: 93 APFKDVDS-KSWYRGYVGALVESGITEGTSAT---TFSPNSNVTREELVVFFIRAFGL 146 + F D K W + + V +GI G+ PN+ +TR++ V F R L Sbjct: 151 SQFADASQVKGWAKSALETAVANGIFTGSEENGKLNLKPNAAITRQDFAVVFARTLDL 208 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.130 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,195,076,164 Number of extensions: 45983261 Number of successful extensions: 101953 Number of sequences better than 1.0e-05: 142 Number of HSP's gapped: 101639 Number of HSP's successfully gapped: 182 Length of query: 967 Length of database: 757,237,578 Length adjustment: 135 Effective length of query: 832 Effective length of database: 390,699,213 Effective search space: 325061745216 Effective search space used: 325061745216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)