BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0069.p (322 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00077751.1| COG0596: Predicted hydrolases or acyltransfer... 195 1e-48 ref|NP_687698.1| conserved domain protein [Streptococcus agalact... 156 6e-37 ref|NP_687697.1| protein of unknown function [Streptococcus agal... 150 4e-35 ref|NP_687696.1| protein of unknown function [Streptococcus agal... 140 5e-32 ref|NP_388081.1| ybdG [Bacillus subtilis] >gi|7474896|pir||D6974... 115 2e-24 >ref|ZP_00077751.1| COG0596: Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [Methanosarcina barkeri] Length = 329 Score = 195 bits (496), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 111/322 (34%), Positives = 175/322 (54%), Gaps = 15/322 (4%) Query: 13 RKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKT-YGQLVSVDGKNMNVTIQ 71 RK+F KA+I + + + + N E+ ++ YG V VDGKNM + + Sbjct: 7 RKLFK---KALIGIPLALIIIIVLSATTNSFLCHQESKDLENAYGHRVQVDGKNMCIDVA 63 Query: 72 GKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIVA 131 G G++ VVLL G+G+ +P LD +PL L Y V+ E FGYGLS+ ++ R++ENI Sbjct: 64 GSGDQVVVLLSGWGSPSPVLDLRPLAANLDDKYTVITAEYFGYGLSDDTNSDRSVENITY 123 Query: 132 EIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQGGL-----EE 186 E+H L+++ + YTLM HSI+G+YGL Y N Y +EV +FVGID+S + L E Sbjct: 124 ELHRALKEMGYNNYTLMAHSISGLYGLYYANMYPNEVKSFVGIDTSVANEDDLEASMQES 183 Query: 187 LPAG-AYKLLKNSGFYRLLVKLNPDQIIAPAV----DAETKAQIRMLSLKNMMNPNIISE 241 L G + L G RLL ++P II A E + +R + L +++ N ++E Sbjct: 184 LSYGNTLRTLNKLGIIRLLSVIDPSSIIPEASGYQRSEEEQELLRKMYLNRLLSQNAMNE 243 Query: 242 SENFKHNFQAAESFSFPADLPVIFFLADDNMDVPD-WVPKHKEQIKDSVHGKVMTLEGEH 300 E + N FP ++PV+FF++ +N++ W KH EQ ++ K++ L G H Sbjct: 244 MEMLEENCNKLSGMKFPDNVPVLFFISSENVETTSGWKEKHVEQFGNNGKNKLIVLNGSH 303 Query: 301 YLHHTRSKEIVQNFRSFMAGIQ 322 YL++ + EI F+ + + Q Sbjct: 304 YLYNEYAPEICNTFKEWDSAEQ 325 >ref|NP_687698.1| conserved domain protein [Streptococcus agalactiae 2603V/R] gb|AAM99570.1| conserved domain protein [Streptococcus agalactiae 2603V/R] Length = 353 Score = 156 bits (394), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 93/279 (33%), Positives = 146/279 (52%), Gaps = 26/279 (9%) Query: 52 IKTYGQLVSVDGKNMNVTIQGKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEP 111 IK YGQ V V GK MNV G+G+ +V +PG GT Y L+ LS ++VVV+EP Sbjct: 84 IKEYGQKVEVKGKKMNVYTVGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEP 143 Query: 112 FGYGLSEVSDKARTIENIVAEIHECLQQLNIH-RYTLMGHSIAGIYGLDYVNQYKDEVTA 170 FG GLS+V D+ R + NI ++IHE LQ++ I +Y + HSI G+Y L Y++ Y EV Sbjct: 144 FGSGLSDVIDQPRNLANITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLG 203 Query: 171 FVGIDSSFPTQGGLEELPAGAYKLLKNSGFYRLLVKLNPDQIIAPAVDAETKAQIRMLSL 230 +G+D+S P G +++ A L + L K+ P V + AQ + Sbjct: 204 LIGLDTSTPGMEGGKQVDFAAPVL-------KELPKI-------PKVSDDINAQFFAIGH 249 Query: 231 KNMMNPNIISESENFKHNFQAAESFSFPADLPVIFFLADD--------NMDVP---DWVP 279 K + N N+ E++N + + ++ P +P ++ LA + ++D+P W Sbjct: 250 KILNNSNMKEEAKNSSNMINESANYKIPKGIPAMYLLARESDLGLQLRSLDIPIKGSWQE 309 Query: 280 KHKEQIKDSVHGKVMTLEGEHYLHHTRSKEIVQNFRSFM 318 +HKE +D + TL+G H ++ T KE++ FM Sbjct: 310 QHKELSEDPKEVSIHTLDGTHQIYDTNLKEVIDYTNQFM 348 >ref|NP_687697.1| protein of unknown function [Streptococcus agalactiae 2603V/R] gb|AAM99569.1| protein of unknown function [Streptococcus agalactiae 2603V/R] Length = 339 Score = 150 bits (379), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 87/289 (30%), Positives = 140/289 (48%), Gaps = 31/289 (10%) Query: 45 SKSEAAKIKTYGQLVSV-DGKNMNVTIQGKGEETVVLLPGYGTAAPGLDYQPLVKELSPF 103 +K +A I+ YGQ + V DGK MN+ G+G+ +V+ PG +P Y+ L+ L Sbjct: 62 TKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQAEISPRYAYKNLLDRLGKK 121 Query: 104 YRVVVIEPFGYGLSEVSDKARTIENIVAEIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQ 163 Y++ +EP GYGLS+++DK RTIENI EIH L+++ + + LM HS+ GIYGL+Y Sbjct: 122 YKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGVKNFYLMAHSLGGIYGLNYAKT 181 Query: 164 YKDEVTAFVGIDSSFPT---QGGLEELPAGAYKLLKNSGFYRLLVKLNPDQIIAPAVDAE 220 Y ++V F+G+D+S P G E A K ++ P VD + Sbjct: 182 YSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES----------------IPEVDKK 225 Query: 221 TKAQIRMLSLKNMMNPNIISESENFKHNFQAAESFSFPADLPVIFFLADDNMD------- 273 T Q + K N + N E FP +P +FLA+++ + Sbjct: 226 TNEQYLSIGAKKSGNKTERDKDRRVSDNLHKMEKVKFPKGMPAKYFLANESAEDIKMRKK 285 Query: 274 ----VPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKEIVQNFRSFM 318 + W +H + +D V TL+ H ++HT+ K + + ++ Sbjct: 286 IFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI 334 >ref|NP_687696.1| protein of unknown function [Streptococcus agalactiae 2603V/R] gb|AAM99568.1| protein of unknown function [Streptococcus agalactiae 2603V/R] Length = 343 Score = 140 bits (352), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 85/293 (29%), Positives = 143/293 (48%), Gaps = 32/293 (10%) Query: 45 SKSEAAKIKTYGQLVSV-DGKNMNVTIQGKGEETVVLLPGYGTAAPGLDYQPLVKELSPF 103 +K +A +K YG+ + V DGK MN+ G+G+ +V +PG +P Y+ L++ LS Sbjct: 67 TKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIVFIPGQAEISPRYAYKNLIERLSKK 126 Query: 104 YRVVVIEPFGYGLSEVSDKARTIENIVAEIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQ 163 Y++ +EP GYGLS++ K RT+ENI EIH L ++ + + L HS+ G+Y L+Y Sbjct: 127 YKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGLNKIGVKNFYLAAHSLGGMYSLNYAKN 186 Query: 164 YKDEVTAFVGIDSSFPTQGGLEEL---PAGAYKLLKNSGFYRLLVKLNPDQIIAPAVDAE 220 Y +EV F+G+D+S P G ++ P A + +KL P VD + Sbjct: 187 YPEEVRGFIGMDTSTPWMEGEQKTKYDPESA----------KWAMKL-------PDVDDK 229 Query: 221 TKAQIRMLSLKNMMNPNIISESENFKHNFQAAESFSFPADLPVIFFLADDNMD------- 273 T Q ++ K N + E E N ++ FP +P +FLAD +++ Sbjct: 230 TNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFLADQSVEGIKLRQA 289 Query: 274 ----VPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKEIVQNFRSFMAGIQ 322 + +W +H + +D V +G H + T+ + + + F+ Q Sbjct: 290 DFKGLKNWDQQHIDLSEDPKDASVEIFKGSHNIFQTQYEHMAKAIDGFIQSHQ 342 >ref|NP_388081.1| ybdG [Bacillus subtilis] pir||D69747 hypothetical protein ybdG - Bacillus subtilis emb|CAB11993.1| ybdG [Bacillus subtilis subsp. subtilis str. 168] Length = 296 Score = 115 bits (287), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 79/271 (29%), Positives = 131/271 (48%), Gaps = 11/271 (4%) Query: 12 MRKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKTYGQLVSVDGKNMNVTIQ 71 M+ ++ L +SL L L +V+I + EAA +K G +V VDGK MNV + Sbjct: 1 MKTLWKVLKIVFVSLAALVLLVSVSVFIYHHFQLNKEAALLKGKGTVVDVDGKKMNVYQE 60 Query: 72 GKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIVA 131 G G++T V + G G AAP + + L + S ++ V++ GYG SEVS R I+ ++ Sbjct: 61 GSGKDTFVFMSGSGIAAPAYEMKGLYSKFSKENKIAVVDRAGYGYSEVSHDDRDIDTVLE 120 Query: 132 EIHECLQQL-NIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQ------GGL 184 + + L + N Y LM HSI+GI + + +Y E+ A + +D P Q G+ Sbjct: 121 QTRKALMKSGNKPPYILMPHSISGIEAMYWAQKYPKEIKAIIAMDIGLPQQYVTYKLSGV 180 Query: 185 EELPAGAYKLLKNSGFYRLL--VKLNPDQIIAPAVDAETKAQIRMLSLKNMMNPNIISES 242 + L + LL + GF+R + NP+ I + E K + ++ K N ++ E Sbjct: 181 DRLKVRGFHLLTSIGFHRFIPSAVYNPEVIRQSFLTDEEKEIYKAINFKQFFNADMEHEL 240 Query: 243 ENFKHNFQAAESFSFPADLPVIFF--LADDN 271 N + + P + PV+ ++D N Sbjct: 241 LQSYQNGSKSVNLPAPKETPVLILDAVSDQN 271 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 473,457,967 Number of extensions: 19723022 Number of successful extensions: 67279 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 67392 Number of HSP's successfully gapped: 37 Length of query: 322 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 196 Effective length of database: 415,135,104 Effective search space: 81366480384 Effective search space used: 81366480384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)