BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0027.p (69 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_691392.1| hypothetical protein OB0471 [Oceanobacillus ihe... 75 3e-13 ref|NP_781350.1| conserved protein [Clostridium tetani E88] >gi|... 59 3e-08 ref|NP_349334.1| Hypothetical protein, CF-30 family [Clostridium... 57 1e-07 >ref|NP_691392.1| hypothetical protein OB0471 [Oceanobacillus iheyensis HTE831] dbj|BAC12427.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 246 Score = 75.5 bits (184), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 36/65 (55%), Positives = 42/65 (64%) Query: 5 TFAIDPGGIRRCLFRNTYIWLNNGEQFWFFPVFVGRTSIAXXXXXXXXXXXXXIDLNQIR 64 TFA+DPGGIRRCLFR TYIW G FW+FP FVGR SIA +DL++I Sbjct: 182 TFAVDPGGIRRCLFRFTYIWPERGRGFWYFPTFVGRNSIAGFRWSGFRWVYFGMDLDRIE 241 Query: 65 SFTCF 69 SF C+ Sbjct: 242 SFQCY 246 >ref|NP_781350.1| conserved protein [Clostridium tetani E88] gb|AAO35287.1| conserved protein [Clostridium tetani E88] Length = 120 Score = 59.3 bits (142), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 32/67 (47%), Positives = 34/67 (50%) Query: 3 ATTFAIDPGGIRRCLFRNTYIWLNNGEQFWFFPVFVGRTSIAXXXXXXXXXXXXXIDLNQ 62 A A+DPG IR C FR YIW G FW F FVG SIA IDLN+ Sbjct: 54 AQILAVDPGSIRPCRFRFVYIWPERGRPFWMFITFVGPRSIAGWRWTGFSWSYFGIDLNR 113 Query: 63 IRSFTCF 69 I SF CF Sbjct: 114 IDSFVCF 120 >ref|NP_349334.1| Hypothetical protein, CF-30 family [Clostridium acetobutylicum] pir||G97235 hypothetical protein CAC2728 [imported] - Clostridium acetobutylicum gb|AAK80674.1| Hypothetical protein, CF-30 family [Clostridium acetobutylicum] Length = 210 Score = 57.0 bits (136), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 29/62 (46%), Positives = 34/62 (54%) Query: 7 AIDPGGIRRCLFRNTYIWLNNGEQFWFFPVFVGRTSIAXXXXXXXXXXXXXIDLNQIRSF 66 A+D G IR C ++ TYIWLNN FW + FVG SIA +DL QIRSF Sbjct: 148 AVDSGAIRMCRYKYTYIWLNNRRSFWAYLTFVGPRSIAGYRWNGRRWIYFGLDLRQIRSF 207 Query: 67 TC 68 C Sbjct: 208 VC 209 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.335 0.146 0.508 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 82,462,632 Number of extensions: 1991390 Number of successful extensions: 4439 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 4439 Number of HSP's successfully gapped: 3 Length of query: 69 Length of database: 757,237,578 Length adjustment: 40 Effective length of query: 29 Effective length of database: 648,633,618 Effective search space: 18810374922 Effective search space used: 18810374922 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 120 (50.8 bits)