BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0025.p (389 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO65246.1| nattokinase precursor [Bacillus subtilis] 276 4e-73 emb|CAD62180.1| subtilisin precursor [Bacillus subtilis] 276 5e-73 sp|P35835|SUBN_BACNA Subtilisin NAT precursor >gi|322200|pir||JH... 276 6e-73 pir||SUBSI subtilisin (EC 3.4.21.62) E precursor - Bacillus subt... 276 9e-73 sp|P00783|SUBT_BACSA Subtilisin amylosacchariticus precursor >gi... 270 4e-71 >gb|AAO65246.1| nattokinase precursor [Bacillus subtilis] Length = 381 Score = 276 bits (707), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 167/383 (43%), Positives = 230/383 (60%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E ++ + E +SV PYGI Sbjct: 66 VQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDH-----IAHEYAQSV----PYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG Sbjct: 177 TIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPT 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A +GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGSSFYYGKGLINVQAA 379 >emb|CAD62180.1| subtilisin precursor [Bacillus subtilis] Length = 381 Score = 276 bits (706), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 165/383 (43%), Positives = 228/383 (59%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E E +++PYGI Sbjct: 66 VQKQFKYVNAAAATLDAKAVKELKQDPSVAYVE---------EDHIAHQYAQSVPYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 +AA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI +KMD+IN+S GG + Sbjct: 177 TVAALNNSIGVLGVAPNASLYAVKVLDSTGNGQYSWIINGIEWAISNKMDVINMSLGGPS 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMG-DDTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + A +GI+++AAAGN+G G TI YPA+Y + IAVGA++SSN+R FS Sbjct: 237 GSTALKSVVDRAVASGIVVVAAAGNEGTSGSSSTIGYPAKYPSTIAVGAVNSSNQRGSFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + GP+LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SVGPELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 EST LG YG G++ + A Sbjct: 357 ESTTTYLGNSFYYGKGLINVQAA 379 >sp|P35835|SUBN_BACNA Subtilisin NAT precursor pir||JH0778 subtilisin (EC 3.4.21.62) NAT precursor - Bacillus subtilis (strain natto NC2-1) gb|AAC60424.1| subtilisin NAT [Bacillus subtilis var. natto] dbj|BAA04989.1| subtilisin [Bacillus subtilis] gb|AAK54130.1| serkinase-nattokinase [Bacillus subtilis var. natto] prf||1903156A subtilisin:ISOTYPE=NAT Length = 381 Score = 276 bits (706), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 165/383 (43%), Positives = 227/383 (59%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E E +++PYGI Sbjct: 66 VQKQFKYVNAAAATLDEKAVKELKKDPSVAYVE---------EDHIAHEYAQSVPYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG Sbjct: 177 TIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPT 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A +GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGNSFYYGKGLINVQAA 379 >pir||SUBSI subtilisin (EC 3.4.21.62) E precursor - Bacillus subtilis gb|AAA22742.1| subtilisin (gtg start codon) Length = 381 Score = 276 bits (705), Expect = 9e-73, Method: Compositional matrix adjust. Identities = 164/383 (42%), Positives = 227/383 (59%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E E +++PYGI Sbjct: 66 VQKQFKYVNAAAATLDEKAVKELKKDPSVAYVE---------EDHIAHEYAQSVPYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GV+P A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG Sbjct: 177 TIAALNNSIGVLGVSPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPT 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A +GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGNSFYYGKGLINVQAA 379 >sp|P00783|SUBT_BACSA Subtilisin amylosacchariticus precursor pir||SUBSS subtilisin (EC 3.4.21.62) amylosacchariticus precursor - Bacillus subtilis dbj|BAA00186.1| subtilisin amylosacchariticus precursor [Bacillus subtilis] Length = 381 Score = 270 bits (691), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 164/383 (42%), Positives = 228/383 (59%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E E +++PYGI Sbjct: 66 VQKQFKYVNAAAATLDEKAVKELKKDPSVAYVE---------EDHIAHEYAQSVPYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GV+P A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG + Sbjct: 177 TIAALNNSIGVLGVSPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPS 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGDD-TILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A +GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSSSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGNSFYYGKGLINVQAA 379 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 593,348,097 Number of extensions: 25735672 Number of successful extensions: 60875 Number of sequences better than 1.0e-05: 1035 Number of HSP's gapped: 58906 Number of HSP's successfully gapped: 1060 Length of query: 389 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 262 Effective length of database: 412,420,005 Effective search space: 108054041310 Effective search space used: 108054041310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 127 (53.5 bits)