BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0021.p (450 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_645128.1| conserved hypothetical protein [Staphylococcus ... 397 e-109 ref|NP_370858.1| conserved hypothetical protein [Staphylococcus ... 397 e-109 ref|NP_470326.1| conserved hypothetical protein [Listeria innocu... 328 2e-88 ref|NP_464515.1| conserved hypothetical protein [Listeria monocy... 316 7e-85 ref|NP_267640.1| hypothetical protein L112499 [Lactococcus lacti... 301 1e-80 >ref|NP_645128.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus MW2] dbj|BAB94176.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus MW2] Length = 451 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 212/450 (47%), Positives = 300/450 (66%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M E LYYFEK+PV KA+ HF++PMM+GT ++VIY ILN YF+G Sbjct: 1 MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIGFLEDSHMISAISLTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 I+M LGNL G+G+GT+ISRLLG K Y K VSSF+ Y + LGLI++ V LP + Sbjct: 61 VFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYGGIALGLIVILVTLPFSDQ 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I LGA ++ T +Y+ VM + +PFV+LFF LE R+ GA M SMIGM+ SV +NI Sbjct: 121 IAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFARAIGAPMISMIGMLASVGLNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILD ++IF F +V+G A T ISN+ A++++ + S +++++K K K++L + Sbjct: 181 ILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMKNSDVVSVNIKLAKPNKEMLSEI 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIG+P F+MSI +G L+ N FL YG+ A+A+YGIS RL+QFPE I+MGLCEGVVPL Sbjct: 241 FKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASYGISFRLVQFPELIIMGLCEGVVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IA++F A+K RMK+ I I +I + VV I V+ I H++GLFT D ++EM ++IL Sbjct: 301 IAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAVFTIGHHMVGLFTTDQDIVEMATFILK 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VT SL + G+ LFTG+ QAT QG A IM+I+QG +IPVL+I N + G GV+WSL+ Sbjct: 361 VTMTSLLLNGIGFLFTGMLQATGQGRGATIMAILQGAIIIPVLFIMNALFGLTGVIWSLL 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IA++ L +++Y+LR++L + L++ Sbjct: 421 IAESLCALAAMLIVYLLRDRLTVDTSELIE 450 >ref|NP_370858.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] ref|NP_373569.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus N315] pir||H89798 conserved hypothetical protein SA0323 [imported] - Staphylococcus aureus (strain N315) dbj|BAB41547.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus N315] dbj|BAB56496.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] Length = 451 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 212/450 (47%), Positives = 300/450 (66%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M E LYYFEK+PV KA+ HF++PMM+GT ++VIY ILN YF+G Sbjct: 1 MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIGFLEDSHMISAISLTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 I+M LGNL G+G+GT+ISRLLG K Y K VSSF+ Y + LGLI++ V LP + Sbjct: 61 VFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYGGIALGLIVILVTLPFSDQ 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I LGA ++ T +Y+ VM + +PFV+LFF LE R+ GA M SMIGM+ SV +NI Sbjct: 121 IAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFARAIGAPMISMIGMLASVGLNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILD ++IF F +V+G A T ISN+ A++++ + S +++++K K K++L + Sbjct: 181 ILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMKNSDVVSVNIKLAKPNKEMLSEI 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIG+P F+MSI +G L+ N FL YG+ A+A+YGIS RL+QFPE I+MGLCEGVVPL Sbjct: 241 FKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASYGISFRLVQFPELIIMGLCEGVVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IA++F A+K RMK+ I I +I + VV I V+ I H++GLFT D ++EM ++IL Sbjct: 301 IAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAVFTIGHHMVGLFTTDQAIVEMATFILK 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VT SL + G+ LFTG+ QAT QG A IM+I+QG +IPVL+I N + G GV+WSL+ Sbjct: 361 VTMASLLLNGIGFLFTGMLQATGQGRGATIMAILQGAIIIPVLFIMNALFGLTGVIWSLL 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IA++ L +++Y+LR++L + L++ Sbjct: 421 IAESLCALAAMLIVYLLRDRLTVDTSELIE 450 >ref|NP_470326.1| conserved hypothetical protein [Listeria innocua] pir||AD1556 conserved hypothetical protein lin0989 [imported] - Listeria innocua (strain Clip11262) emb|CAC96220.1| lin0989 [Listeria innocua] Length = 453 Score = 328 bits (840), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 178/440 (40%), Positives = 274/440 (62%), Gaps = 2/440 (0%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M + YY KA + KA+AH ++PMMLG S+ VIY+I+NA+F+G Sbjct: 1 MKHSDNYYLTKASIPKAIAHLSIPMMLGMSVGVIYNIINAFFIGLLHDTSMLTAVTLGLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 I+MA+GN+ G+G GT+ISRLLG++ K VS+F Y SLVLG+I + LI Sbjct: 61 MFTILMAIGNMFGVGGGTYISRLLGKEDGIKAKQVSAFVLYGSLVLGIICAIILGFLINP 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 + H LGA SF TK+Y +LI SPF++ F LE +VR+EGA+ SM GM++ +VN+ Sbjct: 121 VTHFLGADATSFLHTKNYTLALLICSPFIIANFALEQVVRAEGASRISMNGMLIGTLVNL 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 + D L+I F+++V+G A + I++ V+ IYYA+++ KS +L+I KWFKATK+I+ NV Sbjct: 181 VFDPLLILYFNFNVVGAAVSIGIASTVSLIYYAWYLEKKSDYLSIRFKWFKATKEIVQNV 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIGV ++S+FL +L+ NH+ + YG+ VA +G++ R++Q PEFI MGL G++PL Sbjct: 241 FKIGVSELLLSLFLIVTTLVLNHYSMIYGEGVVAGFGVALRVVQLPEFICMGLYMGIIPL 300 Query: 301 IAFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYIL 359 +A++F+A + R + I FT +I + +V V++ ++ LF++ +I +G +I+ Sbjct: 301 LAYNFSAGNIARFEKAIRFTAVSIGLIVLVISSFVFIFRFQVMHLFSDSQSVISLGVHIM 360 Query: 360 HVTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSL 419 +S +G T LFT FQA + A IMS+ QG+ IPV+ + G GV+WSL Sbjct: 361 VAMLISSLFSGFTGLFTSTFQAIGKAIPATIMSVSQGIIFIPVIILGQHYFGLMGVIWSL 420 Query: 420 VIADAAAFLVGAVMLYVLRN 439 + L+G V+L+ + N Sbjct: 421 TTTEILTCLIG-VILFTIHN 439 >ref|NP_464515.1| conserved hypothetical protein [Listeria monocytogenes EGD-e] pir||AF1198 conserved hypothetical protein lmo0990 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC99068.1| lmo0990 [Listeria monocytogenes] Length = 453 Score = 316 bits (810), Expect = 7e-85, Method: Compositional matrix adjust. Identities = 175/441 (39%), Positives = 274/441 (62%), Gaps = 4/441 (0%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M + YY KA + KA+AH ++PMMLG S+ VIY+I+NA+F+G Sbjct: 1 MKHSDNYYLTKASIPKAIAHLSIPMMLGMSVGVIYNIVNAFFIGLLHDTSMLTAVTLGLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLP-LIE 119 I+MA+GN+ G+G GT+ISRLLG++ K VS+F Y SLVLG IL A+ L LI Sbjct: 61 MFTILMAIGNMFGVGGGTYISRLLGKEEGTKAKQVSAFVLYGSLVLG-ILCAILLGFLIN 119 Query: 120 LIVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVN 179 + H LGA SF TK+Y +LI SPF++ F LE +VR+EGA+ SM GM++ VVN Sbjct: 120 PVTHFLGADATSFLHTKNYTLALLICSPFIIANFALEQVVRAEGASRVSMNGMLIGTVVN 179 Query: 180 IILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGN 239 ++ D L+I F ++V+G A + +++L + IYYA+++ KS +L+I KWF+A+K+I+ N Sbjct: 180 LVFDPLLILYFDFNVVGAAVSVGLASLFSLIYYAWYLEKKSDYLSIRFKWFQASKEIVQN 239 Query: 240 VFKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVP 299 VFKIGV ++S+FL +L+ NH+ + YG+ VA +G++ R++Q PEFI MGL G++P Sbjct: 240 VFKIGVSELLLSLFLIVTTLVLNHYSMIYGEGVVAGFGVALRVVQLPEFICMGLYMGIIP 299 Query: 300 LIAFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYI 358 L+A+++ + + R + I FT +I + ++ +V++ ++ LF++ +I +G +I Sbjct: 300 LLAYNYGSGNIARFEKAIRFTAISIGLIVLLLSSLVFIFRFQVMHLFSDSQSVITLGVHI 359 Query: 359 LHVTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWS 418 + +S +G T LFT FQA + A IMS+ QG+ IPV+ + G GV+WS Sbjct: 360 MVAMLISSLFSGFTGLFTSTFQAIGKAIPATIMSVSQGIIFIPVIILGQHYFGLMGVIWS 419 Query: 419 LVIADAAAFLVGAVMLYVLRN 439 L + ++G V L+ + N Sbjct: 420 LTATEILTCIIG-VTLFTIHN 439 >ref|NP_267640.1| hypothetical protein L112499 [Lactococcus lactis subsp. lactis] pir||D86810 hypothetical protein ypbC [imported] - Lactococcus lactis subsp. lactis (strain IL1403) gb|AAK05582.1| HYPOTHETICAL PROTEIN [Lactococcus lactis subsp. lactis] Length = 459 Score = 301 bits (772), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 169/453 (37%), Positives = 270/453 (59%), Gaps = 9/453 (1%) Query: 2 DAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXXX 61 N YY ++AP+ KA+AH ++PMM+G S IY+++NAYF+G Sbjct: 3 KKSNSYYLKEAPIRKAIAHLSIPMMIGISATTIYNLINAYFIGLVHDTNMMSTITLGLTI 62 Query: 62 XXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIELI 121 + MA+GNL G+G GT+I+RLLG + K ++ ++FY SL++GL++ V L + I Sbjct: 63 TIVFMAIGNLFGVGGGTYITRLLGSGDKEKGKKIAGYSFYMSLIIGLLIGLVSLFFLSPI 122 Query: 122 VHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNII 181 H LG+ + +TK+Y V+L G ++L + LE LVR+EGA+ SM GM + V +I+ Sbjct: 123 AHILGSDAATLTYTKEYAAVLLGGGFSMILNYGLEQLVRAEGASKESMYGMFVGVATSIV 182 Query: 182 LDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNVF 241 LDAL I V + VIG + VI+NL ++ YY + + +KS+ L +K FK + ++ Sbjct: 183 LDALFILVLNLHVIGAGLSMVIANLASTAYYVWFLENKSESLKGFLKHFKISLKDQFEIY 242 Query: 242 KIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPLI 301 KIG+ S F+ +L+ NHF V+YGD +A++GI+ R+ Q PEF+ MGL G +PL Sbjct: 243 KIGIAEVFKSAFMIVTTLLLNHFSVQYGDNVLASFGIALRITQLPEFLTMGLFLGAIPLF 302 Query: 302 AFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 A+SF ++ R+K + I G++++F ++VYL ++ LFT+D +I++G +L Sbjct: 303 AYSFASNNTQRLKEALKELTLWIGGISILFALIVYLFRVPVMQLFTDDSAVIKVGLIVLA 362 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 +S G + LFTG+F+A+ G +MS IQG+ IP++ I + G G++WS+ Sbjct: 363 AQLVSSVFNGFSGLFTGIFEASGLGAQTGVMSTIQGILYIPIVIILHHFYGLTGLIWSMT 422 Query: 421 IADAAAFLVGAVML--YVLR---NKL---QPEL 445 + + AF+ G +ML Y+L+ KL QPEL Sbjct: 423 LTEGIAFVAGLIMLIPYLLKLNNGKLMENQPEL 455 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.143 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 571,720,054 Number of extensions: 22173199 Number of successful extensions: 106252 Number of sequences better than 1.0e-05: 630 Number of HSP's gapped: 105345 Number of HSP's successfully gapped: 632 Length of query: 450 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 321 Effective length of database: 406,989,807 Effective search space: 130643728047 Effective search space used: 130643728047 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)