BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0014.p (449 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00020323.1| hypothetical protein [Chloroflexus aurantiacus] 187 5e-46 ref|NP_244547.1| sugar ABC transporter (sugar-binding protein) [... 156 9e-37 ref|ZP_00056970.1| COG1653: ABC-type sugar transport system, per... 132 1e-29 ref|NP_694045.1| sugar ABC transporter sugar-binding protein [Oc... 131 3e-29 ref|NP_623882.1| Sugar-binding periplasmic proteins/domains [The... 123 1e-26 >ref|ZP_00020323.1| hypothetical protein [Chloroflexus aurantiacus] Length = 347 Score = 187 bits (475), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 112/350 (32%), Positives = 173/350 (49%), Gaps = 10/350 (2%) Query: 103 NEMPAMFDTDPDAYASKLVQQGKLVDMKKLLEELGKYQDFRP--VALQYQQFADGSMYTF 160 + +P MF+ + A L GK++++++ +ELG Y P V+L +Y Sbjct: 1 DSLPFMFNYESGAPLRDLAASGKVLELEQTFKELGIYDRLNPGAVSLLKNLVGGSGLYAL 60 Query: 161 PLEYGIEVFWYNIKMFKDLGLEPPKTFDEFMKVAETLKQSGVTPIAVDGKDKWPVLRYLA 220 PLE IE FWYN ++F + L P T+DE + VA+ L Q+G+ P AV G KWP+ R + Sbjct: 61 PLELNIEGFWYNKQIFAEHNLAVPTTWDELLAVADKLHQAGIQPFAVSGLQKWPLTRLIN 120 Query: 221 YKPFRMTGNDFIENVKQGKASFKDPAGMEGIKFVHDLGTKGYFQEGFTATDYTAARDLFL 280 R+ G D + V G+ D + + V D+ KGYF +G DY A+DLFL Sbjct: 121 GYVMRVYGYDVMSRVVNGELKVTDAGFIAAAQIVQDMAMKGYFGQGVNTLDYAPAQDLFL 180 Query: 281 GGKAGMYYMGSWETMNFVNDKVSTEMKDNIGFFRLPMMEGAKTAENDYFINSGIGVAFNQ 340 G+A MYYMGSW +F N +++ DNIGFF +P++EG NDY IN+G+ +F+Q Sbjct: 181 QGRAAMYYMGSWALRDFNNPELNKIGIDNIGFFNIPLVEGGVGTLNDYSINAGLTTSFSQ 240 Query: 341 ETFDDTMK---EFVKFLLEQYP-KAYTEKGQFSPFNYSLDQEQGMPELFYKIQDEIAKSG 396 + F + E++K + Y +A +E G + F S + P + EI Sbjct: 241 KEFSRNPQGYGEWMKAVFSAYGDRAMSELGMITGFTVSQIPDNVSPLTRLTVA-EIDNVK 299 Query: 397 NVFAVPW-DTRLDPATXXXXXXXXXXXXXXXHTPDDFAELMDQAIKENAP 445 N PW + + T +P+++ + +AI E P Sbjct: 300 N--GAPWFEAYFNARTQAVATDNVQLLVTGDMSPEEYMAELQKAIDEQLP 347 >ref|NP_244547.1| sugar ABC transporter (sugar-binding protein) [Bacillus halodurans] pir||H84109 sugar ABC transporter (sugar-binding protein) BH3680 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07399.1| sugar ABC transporter (sugar-binding protein) [Bacillus halodurans] Length = 438 Score = 156 bits (395), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 106/382 (27%), Positives = 184/382 (48%), Gaps = 12/382 (3%) Query: 65 VKEIADKFSQTHPEFELVLEGTGDRPSYLQKLRTLIASNEMPAMFDTDPDAYASKLVQQG 124 V +I +F +P + LE + Y KL+ + +SN++P + T P + V Sbjct: 61 VNQIISEFEADNPHITVELE-VLENEQYKNKLQVISSSNQLPDVGMTWPAGFLEPYVNGK 119 Query: 125 KLVDMKKLLEELGKYQDFRPVALQYQQFADGSMYTFPLEYGIEVFWYNIKMFKDLGLEPP 184 + +LE+ G + F ++ + DG Y PLE I +YN +F + GLE P Sbjct: 120 LFTPLDDILED-GLREQFVAGTVEAYEI-DGQAYALPLELNIAPVFYNKAIFDEYGLEAP 177 Query: 185 KTFDEFMKVAETLKQSGVTPIAVDGKDKWPVLRYLAYKPFRMTGNDFIENVKQGKASFKD 244 +T++EF+ V +TL ++GVTPIA+ +D+W + Y R+ G D + + SF+D Sbjct: 178 ETYEEFIHVVDTLVENGVTPIALGNRDRWTGSLWYMYMAERIGGADALNSAIDRSGSFED 237 Query: 245 PAGMEGIKFVHDLGTKGYFQEGFTATDYTAARDLFLGGKAGMYYMGSWETMNF-VNDKVS 303 ++ + +L + F GF A+ F+ G A MY +GSW+ N+ N++V Sbjct: 238 ERLIDAAAHIQELIDRDAFIRGFNGLSDQEAKAEFMNGGAAMYLIGSWDLPNYTTNEEVP 297 Query: 304 TEMKDNIGFFRLPMMEGAKTAENDYFINSGIG--VAFNQETFDDTMKEFVKFLLEQYPKA 361 E +D I FF+ P ++G + N + G+G VA N + ++ K+FVK+ +E++ K Sbjct: 298 QEFRDGISFFKFPTIDGGEGDINSWVGGPGVGLFVAENSDVKEEA-KQFVKYFIEEWGKY 356 Query: 362 YTEKGQFSPFNYSLDQEQGMPELFYKIQDEIAKSGNV--FAVPWDTRLDPATXXXXXXXX 419 E P E +P+LF + DE+ + ++ FA D ++ + Sbjct: 357 SVEDAGVIPGTQVNTDEVDLPDLFIDVLDELNHASDITLFA---DVQMSASVAETHLNMI 413 Query: 420 XXXXXXXHTPDDFAELMDQAIK 441 TP+ FAE ++A+ Sbjct: 414 QSLFGHEVTPEAFAEEHEKALS 435 >ref|ZP_00056970.1| COG1653: ABC-type sugar transport system, periplasmic component [Thermobifida fusca] Length = 434 Score = 132 bits (333), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 100/375 (26%), Positives = 167/375 (44%), Gaps = 48/375 (12%) Query: 91 SYLQKLRTLIASNEMPAMFDTDPDAYASKLVQQGKLVDMKKLLEELGKYQD-FRPVALQY 149 +Y Q+++ + S E P +F ++ V+ ++VD+ + +E +++D F P L Sbjct: 77 AYKQRIQVALGSPESPDVFFNWGGGNLAQYVKANQVVDLTEAMEANPEFRDAFLPSVLDV 136 Query: 150 QQFADGSMYTFPLEYGIEV-FWYNIKMFKDLGLEPPKTFDEFMKVAETLKQSGVTPIAVD 208 + DGS Y P++ + V +YN K+FK++G+EPP T+ E + + +T K+ G+TPI + Sbjct: 137 AKI-DGSYYGVPMQGVLPVVLFYNKKVFKEVGVEPPTTYSELLDLVDTFKKEGITPIVLP 195 Query: 209 GKDKWPVLRYLAYKPFRMTGNDFIENVKQGK-ASFKDPAGMEGIKFVHDLGTKGYFQEGF 267 G W L +L Y R+ G + + + +GK ++KDPA ++ ++ DL +G F + F Sbjct: 196 GAQAWTELMWLEYLTDRIGGAEVFQAIAEGKEGAWKDPAIIKALEMCQDLVERGAFGDNF 255 Query: 268 TA--TDYTAARDLFLGGKAGMYYMGSWETMNFVNDKVSTEMKDNIGFFRLPMMEGAKTAE 325 ++ +D +A + GK MY MG+WE N N+GF P +E K Sbjct: 256 SSLTSDNGSASTVLASGKGAMYLMGTWEISNQQEHNPDFLADGNLGFIAFPALEDGKGDP 315 Query: 326 NDYFINSGIGVAFNQETFDDTMKEFVKFLLEQYPK-----------------------AY 362 N + N ++ D + E + FL+E A Sbjct: 316 AAIVGNPSNYFSVNSDS--DNVDEAIDFLVETMASDAYVEDLIEIGHVPAVAGIEDKLAE 373 Query: 363 TEKGQFSPFNYSLDQEQGMPELFYKIQDEIAKSGNVFAVPWDTRLDPATXXXXXXXXXXX 422 TE +F+ F Y L E A S F WD + PA Sbjct: 374 TEHSEFTTFTYQLVAE--------------APS---FTQSWDQAIPPAAAETMLTNLQLV 416 Query: 423 XXXXHTPDDFAELMD 437 TP++FAE M+ Sbjct: 417 FLGDMTPEEFAEAME 431 >ref|NP_694045.1| sugar ABC transporter sugar-binding protein [Oceanobacillus iheyensis HTE831] dbj|BAC15079.1| sugar ABC transporter sugar-binding protein [Oceanobacillus iheyensis HTE831] Length = 435 Score = 131 bits (330), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 94/341 (27%), Positives = 166/341 (48%), Gaps = 12/341 (3%) Query: 64 TVKEIADKFSQTHPEFELVLEGTGDRPSYLQKLRTLIASNEMPAMFDTDPDAYASKLVQQ 123 V +I + F + ++ LE + Y +K++ SNE+P + T Y V Sbjct: 56 VVNQIIEDFETENEGIDIELEVLSNE-QYKEKIKVQSTSNELPDVGMTWAAGYMEPFVDG 114 Query: 124 GKLVDMKKLLEELGKYQDFRPVALQYQQFA-DGSMYTFPLEYGIEVFWYNIKMFKDLGLE 182 + +LEE ++F VA F+ D + Y PLE I +YN +F + GLE Sbjct: 115 NMFASLDDILEEDNLSEEF--VAGTTDAFSIDDTAYGLPLELNISSIYYNKAIFDEYGLE 172 Query: 183 PPKTFDEFMKVAETLKQSGVTPIAVDGKDKWPVLRYLAYKPFRMTGN-DFIENVKQGKAS 241 P T DEF +V +TL ++ V P+A+ +D W + Y R+ G+ D + + S Sbjct: 173 EPTTLDEFNQVVDTLNENDVIPVALGNRDAWTGSMWYMYLANRLGGDEDVLTSAIDRSGS 232 Query: 242 FKDPAGMEGIKFVHDLGTKGYFQEGFTATDYTAARDLFLGGKAGMYYMGSWETMNF-VND 300 F+DPA ++ + + L F GF A+ +F+ +A MY + +W+ N+ N+ Sbjct: 233 FEDPALLKAAEHLQGLVNDNSFVSGFNGLADQEAKSMFMESQAAMYLIATWDLPNYTTNE 292 Query: 301 KVSTEMKDNIGFFRLPMMEGAKTAENDYFINSGIG--VAFNQETFDDTMKEFVKFLLEQY 358 V E +D++G+F P ++G+ N Y G+G V+ N E ++ K FV + ++++ Sbjct: 293 DVPQEFRDSVGYFPFPQVDGSGDF-NSYVGGPGVGLFVSENSEVKEEA-KTFVSYFVKEW 350 Query: 359 -PKAYTEKGQFSPFNYSLDQEQGMPELFYKIQDEIAKSGNV 398 +A TE G P + +PEL+ ++ D++ + N+ Sbjct: 351 GERAVTEVG-IIPATKIDGEALDLPELYVEVLDDLNNASNI 390 >ref|NP_623882.1| Sugar-binding periplasmic proteins/domains [Thermoanaerobacter tengcongensis] gb|AAM25486.1| Sugar-binding periplasmic proteins/domains [Thermoanaerobacter tengcongensis] Length = 453 Score = 123 bits (308), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 89/308 (28%), Positives = 145/308 (47%), Gaps = 21/308 (6%) Query: 68 IADKFSQTHPEFELVLEGTGDRPSYLQKLRTLIASNEMPAMFDT--DPDAYA----SKLV 121 + D++ + HP ++ +E D P Y QK + ASNEMP +F P + + LV Sbjct: 74 LIDEYMKEHPNVKIEVEALQDEP-YKQKFKVYAASNEMPDIFMVWGQPSFFLPVMKAGLV 132 Query: 122 QQGKLVDMKKLLEELGKYQDFRPVALQYQQFADGSMYTFPLEYGIEVFWYNIKMFKDLGL 181 ++ K+ +K + +DF +G +Y P V +YN +F + Sbjct: 133 EEIKMDQIKDYGFKTSSLKDF---------MYEGKLYGLPRNTDFMVLYYNKGLFDKYSV 183 Query: 182 EPPKTFDEFMKVAETLKQSGVTPIAVDGKDKWPVLRYLAYKPFRMTGN-DFIENVKQGKA 240 P TFDE + +T +++ + PI+++GKDKW + + +G+ ++I V K Sbjct: 184 NVPNTFDELLGAVKTFRKNNIAPISINGKDKWSLAILYQDLVLKESGDQEYIYRVLSEKN 243 Query: 241 SF-KDPAGMEGIKFVHDLGTKGYFQEGFTATDYTAARDLFLGGKAGMYYMGSWETMNFVN 299 + KDP ++ K +L G FQ+ F A DY AA +LF KA MYYMGSWE N Sbjct: 244 NISKDPILLKAAKDFVELVNAGGFQDSFIAADYGAANNLFAQEKAAMYYMGSWEVGMATN 303 Query: 300 DKVSTEMKDNIGFFRLPMMEGAKTAENDYFINSGIGVAFNQET-FDDTMKEFVKFLLEQY 358 S K N+ P++ G K D G G A + ++ + + + F++E Sbjct: 304 PNFSDSFKKNLDVTYFPIISGGKAKNTDILAWHGGGYAVSSKSPVKEEAMKLLLFMME-- 361 Query: 359 PKAYTEKG 366 P +T+ G Sbjct: 362 PNRWTKIG 369 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 632,350,601 Number of extensions: 27218277 Number of successful extensions: 60728 Number of sequences better than 1.0e-05: 169 Number of HSP's gapped: 60733 Number of HSP's successfully gapped: 169 Length of query: 449 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 320 Effective length of database: 406,989,807 Effective search space: 130236738240 Effective search space used: 130236738240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)